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Hypothesis Testing | A Step-by-Step Guide with Easy Examples

Published on November 8, 2019 by Rebecca Bevans . Revised on June 22, 2023.

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics . It is most often used by scientists to test specific predictions, called hypotheses, that arise from theories.

There are 5 main steps in hypothesis testing:

• State your research hypothesis as a null hypothesis and alternate hypothesis (H o ) and (H a  or H 1 ).
• Collect data in a way designed to test the hypothesis.
• Perform an appropriate statistical test .
• Decide whether to reject or fail to reject your null hypothesis.
• Present the findings in your results and discussion section.

Though the specific details might vary, the procedure you will use when testing a hypothesis will always follow some version of these steps.

Table of contents

Step 1: state your null and alternate hypothesis, step 2: collect data, step 3: perform a statistical test, step 4: decide whether to reject or fail to reject your null hypothesis, step 5: present your findings, other interesting articles, frequently asked questions about hypothesis testing.

After developing your initial research hypothesis (the prediction that you want to investigate), it is important to restate it as a null (H o ) and alternate (H a ) hypothesis so that you can test it mathematically.

The alternate hypothesis is usually your initial hypothesis that predicts a relationship between variables. The null hypothesis is a prediction of no relationship between the variables you are interested in.

• H 0 : Men are, on average, not taller than women. H a : Men are, on average, taller than women.

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For a statistical test to be valid , it is important to perform sampling and collect data in a way that is designed to test your hypothesis. If your data are not representative, then you cannot make statistical inferences about the population you are interested in.

There are a variety of statistical tests available, but they are all based on the comparison of within-group variance (how spread out the data is within a category) versus between-group variance (how different the categories are from one another).

If the between-group variance is large enough that there is little or no overlap between groups, then your statistical test will reflect that by showing a low p -value . This means it is unlikely that the differences between these groups came about by chance.

Alternatively, if there is high within-group variance and low between-group variance, then your statistical test will reflect that with a high p -value. This means it is likely that any difference you measure between groups is due to chance.

Your choice of statistical test will be based on the type of variables and the level of measurement of your collected data .

• an estimate of the difference in average height between the two groups.
• a p -value showing how likely you are to see this difference if the null hypothesis of no difference is true.

Based on the outcome of your statistical test, you will have to decide whether to reject or fail to reject your null hypothesis.

In most cases you will use the p -value generated by your statistical test to guide your decision. And in most cases, your predetermined level of significance for rejecting the null hypothesis will be 0.05 – that is, when there is a less than 5% chance that you would see these results if the null hypothesis were true.

In some cases, researchers choose a more conservative level of significance, such as 0.01 (1%). This minimizes the risk of incorrectly rejecting the null hypothesis ( Type I error ).

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The results of hypothesis testing will be presented in the results and discussion sections of your research paper , dissertation or thesis .

In the results section you should give a brief summary of the data and a summary of the results of your statistical test (for example, the estimated difference between group means and associated p -value). In the discussion , you can discuss whether your initial hypothesis was supported by your results or not.

In the formal language of hypothesis testing, we talk about rejecting or failing to reject the null hypothesis. You will probably be asked to do this in your statistics assignments.

However, when presenting research results in academic papers we rarely talk this way. Instead, we go back to our alternate hypothesis (in this case, the hypothesis that men are on average taller than women) and state whether the result of our test did or did not support the alternate hypothesis.

If your null hypothesis was rejected, this result is interpreted as “supported the alternate hypothesis.”

These are superficial differences; you can see that they mean the same thing.

You might notice that we don’t say that we reject or fail to reject the alternate hypothesis . This is because hypothesis testing is not designed to prove or disprove anything. It is only designed to test whether a pattern we measure could have arisen spuriously, or by chance.

If we reject the null hypothesis based on our research (i.e., we find that it is unlikely that the pattern arose by chance), then we can say our test lends support to our hypothesis . But if the pattern does not pass our decision rule, meaning that it could have arisen by chance, then we say the test is inconsistent with our hypothesis .

If you want to know more about statistics , methodology , or research bias , make sure to check out some of our other articles with explanations and examples.

• Normal distribution
• Descriptive statistics
• Measures of central tendency
• Correlation coefficient

Methodology

• Cluster sampling
• Stratified sampling
• Types of interviews
• Cohort study
• Thematic analysis

Research bias

• Implicit bias
• Cognitive bias
• Survivorship bias
• Availability heuristic
• Nonresponse bias
• Regression to the mean

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

A hypothesis states your predictions about what your research will find. It is a tentative answer to your research question that has not yet been tested. For some research projects, you might have to write several hypotheses that address different aspects of your research question.

A hypothesis is not just a guess — it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Null and alternative hypotheses are used in statistical hypothesis testing . The null hypothesis of a test always predicts no effect or no relationship between variables, while the alternative hypothesis states your research prediction of an effect or relationship.

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Hypothesis Testing

Hypothesis testing is a tool for making statistical inferences about the population data. It is an analysis tool that tests assumptions and determines how likely something is within a given standard of accuracy. Hypothesis testing provides a way to verify whether the results of an experiment are valid.

A null hypothesis and an alternative hypothesis are set up before performing the hypothesis testing. This helps to arrive at a conclusion regarding the sample obtained from the population. In this article, we will learn more about hypothesis testing, its types, steps to perform the testing, and associated examples.

What is Hypothesis Testing in Statistics?

Hypothesis testing uses sample data from the population to draw useful conclusions regarding the population probability distribution . It tests an assumption made about the data using different types of hypothesis testing methodologies. The hypothesis testing results in either rejecting or not rejecting the null hypothesis.

Hypothesis Testing Definition

Hypothesis testing can be defined as a statistical tool that is used to identify if the results of an experiment are meaningful or not. It involves setting up a null hypothesis and an alternative hypothesis. These two hypotheses will always be mutually exclusive. This means that if the null hypothesis is true then the alternative hypothesis is false and vice versa. An example of hypothesis testing is setting up a test to check if a new medicine works on a disease in a more efficient manner.

Null Hypothesis

The null hypothesis is a concise mathematical statement that is used to indicate that there is no difference between two possibilities. In other words, there is no difference between certain characteristics of data. This hypothesis assumes that the outcomes of an experiment are based on chance alone. It is denoted as \(H_{0}\). Hypothesis testing is used to conclude if the null hypothesis can be rejected or not. Suppose an experiment is conducted to check if girls are shorter than boys at the age of 5. The null hypothesis will say that they are the same height.

Alternative Hypothesis

The alternative hypothesis is an alternative to the null hypothesis. It is used to show that the observations of an experiment are due to some real effect. It indicates that there is a statistical significance between two possible outcomes and can be denoted as \(H_{1}\) or \(H_{a}\). For the above-mentioned example, the alternative hypothesis would be that girls are shorter than boys at the age of 5.

Hypothesis Testing P Value

In hypothesis testing, the p value is used to indicate whether the results obtained after conducting a test are statistically significant or not. It also indicates the probability of making an error in rejecting or not rejecting the null hypothesis.This value is always a number between 0 and 1. The p value is compared to an alpha level, \(\alpha\) or significance level. The alpha level can be defined as the acceptable risk of incorrectly rejecting the null hypothesis. The alpha level is usually chosen between 1% to 5%.

Hypothesis Testing Critical region

All sets of values that lead to rejecting the null hypothesis lie in the critical region. Furthermore, the value that separates the critical region from the non-critical region is known as the critical value.

Hypothesis Testing Formula

Depending upon the type of data available and the size, different types of hypothesis testing are used to determine whether the null hypothesis can be rejected or not. The hypothesis testing formula for some important test statistics are given below:

• z = \(\frac{\overline{x}-\mu}{\frac{\sigma}{\sqrt{n}}}\). \(\overline{x}\) is the sample mean, \(\mu\) is the population mean, \(\sigma\) is the population standard deviation and n is the size of the sample.
• t = \(\frac{\overline{x}-\mu}{\frac{s}{\sqrt{n}}}\). s is the sample standard deviation.
• \(\chi ^{2} = \sum \frac{(O_{i}-E_{i})^{2}}{E_{i}}\). \(O_{i}\) is the observed value and \(E_{i}\) is the expected value.

We will learn more about these test statistics in the upcoming section.

Types of Hypothesis Testing

Selecting the correct test for performing hypothesis testing can be confusing. These tests are used to determine a test statistic on the basis of which the null hypothesis can either be rejected or not rejected. Some of the important tests used for hypothesis testing are given below.

Hypothesis Testing Z Test

A z test is a way of hypothesis testing that is used for a large sample size (n ≥ 30). It is used to determine whether there is a difference between the population mean and the sample mean when the population standard deviation is known. It can also be used to compare the mean of two samples. It is used to compute the z test statistic. The formulas are given as follows:

• One sample: z = \(\frac{\overline{x}-\mu}{\frac{\sigma}{\sqrt{n}}}\).
• Two samples: z = \(\frac{(\overline{x_{1}}-\overline{x_{2}})-(\mu_{1}-\mu_{2})}{\sqrt{\frac{\sigma_{1}^{2}}{n_{1}}+\frac{\sigma_{2}^{2}}{n_{2}}}}\).

Hypothesis Testing t Test

The t test is another method of hypothesis testing that is used for a small sample size (n < 30). It is also used to compare the sample mean and population mean. However, the population standard deviation is not known. Instead, the sample standard deviation is known. The mean of two samples can also be compared using the t test.

• One sample: t = \(\frac{\overline{x}-\mu}{\frac{s}{\sqrt{n}}}\).
• Two samples: t = \(\frac{(\overline{x_{1}}-\overline{x_{2}})-(\mu_{1}-\mu_{2})}{\sqrt{\frac{s_{1}^{2}}{n_{1}}+\frac{s_{2}^{2}}{n_{2}}}}\).

Hypothesis Testing Chi Square

The Chi square test is a hypothesis testing method that is used to check whether the variables in a population are independent or not. It is used when the test statistic is chi-squared distributed.

One Tailed Hypothesis Testing

One tailed hypothesis testing is done when the rejection region is only in one direction. It can also be known as directional hypothesis testing because the effects can be tested in one direction only. This type of testing is further classified into the right tailed test and left tailed test.

Right Tailed Hypothesis Testing

The right tail test is also known as the upper tail test. This test is used to check whether the population parameter is greater than some value. The null and alternative hypotheses for this test are given as follows:

\(H_{0}\): The population parameter is ≤ some value

\(H_{1}\): The population parameter is > some value.

If the test statistic has a greater value than the critical value then the null hypothesis is rejected

Left Tailed Hypothesis Testing

The left tail test is also known as the lower tail test. It is used to check whether the population parameter is less than some value. The hypotheses for this hypothesis testing can be written as follows:

\(H_{0}\): The population parameter is ≥ some value

\(H_{1}\): The population parameter is < some value.

The null hypothesis is rejected if the test statistic has a value lesser than the critical value.

Two Tailed Hypothesis Testing

In this hypothesis testing method, the critical region lies on both sides of the sampling distribution. It is also known as a non - directional hypothesis testing method. The two-tailed test is used when it needs to be determined if the population parameter is assumed to be different than some value. The hypotheses can be set up as follows:

\(H_{0}\): the population parameter = some value

\(H_{1}\): the population parameter ≠ some value

The null hypothesis is rejected if the test statistic has a value that is not equal to the critical value.

Hypothesis Testing Steps

Hypothesis testing can be easily performed in five simple steps. The most important step is to correctly set up the hypotheses and identify the right method for hypothesis testing. The basic steps to perform hypothesis testing are as follows:

• Step 1: Set up the null hypothesis by correctly identifying whether it is the left-tailed, right-tailed, or two-tailed hypothesis testing.
• Step 2: Set up the alternative hypothesis.
• Step 3: Choose the correct significance level, \(\alpha\), and find the critical value.
• Step 4: Calculate the correct test statistic (z, t or \(\chi\)) and p-value.
• Step 5: Compare the test statistic with the critical value or compare the p-value with \(\alpha\) to arrive at a conclusion. In other words, decide if the null hypothesis is to be rejected or not.

Hypothesis Testing Example

The best way to solve a problem on hypothesis testing is by applying the 5 steps mentioned in the previous section. Suppose a researcher claims that the mean average weight of men is greater than 100kgs with a standard deviation of 15kgs. 30 men are chosen with an average weight of 112.5 Kgs. Using hypothesis testing, check if there is enough evidence to support the researcher's claim. The confidence interval is given as 95%.

Step 1: This is an example of a right-tailed test. Set up the null hypothesis as \(H_{0}\): \(\mu\) = 100.

Step 2: The alternative hypothesis is given by \(H_{1}\): \(\mu\) > 100.

Step 3: As this is a one-tailed test, \(\alpha\) = 100% - 95% = 5%. This can be used to determine the critical value.

1 - \(\alpha\) = 1 - 0.05 = 0.95

0.95 gives the required area under the curve. Now using a normal distribution table, the area 0.95 is at z = 1.645. A similar process can be followed for a t-test. The only additional requirement is to calculate the degrees of freedom given by n - 1.

Step 4: Calculate the z test statistic. This is because the sample size is 30. Furthermore, the sample and population means are known along with the standard deviation.

z = \(\frac{\overline{x}-\mu}{\frac{\sigma}{\sqrt{n}}}\).

\(\mu\) = 100, \(\overline{x}\) = 112.5, n = 30, \(\sigma\) = 15

z = \(\frac{112.5-100}{\frac{15}{\sqrt{30}}}\) = 4.56

Step 5: Conclusion. As 4.56 > 1.645 thus, the null hypothesis can be rejected.

Hypothesis Testing and Confidence Intervals

Confidence intervals form an important part of hypothesis testing. This is because the alpha level can be determined from a given confidence interval. Suppose a confidence interval is given as 95%. Subtract the confidence interval from 100%. This gives 100 - 95 = 5% or 0.05. This is the alpha value of a one-tailed hypothesis testing. To obtain the alpha value for a two-tailed hypothesis testing, divide this value by 2. This gives 0.05 / 2 = 0.025.

Related Articles:

• Probability and Statistics
• Data Handling

Important Notes on Hypothesis Testing

• Hypothesis testing is a technique that is used to verify whether the results of an experiment are statistically significant.
• It involves the setting up of a null hypothesis and an alternate hypothesis.
• There are three types of tests that can be conducted under hypothesis testing - z test, t test, and chi square test.
• Hypothesis testing can be classified as right tail, left tail, and two tail tests.

Examples on Hypothesis Testing

• Example 1: The average weight of a dumbbell in a gym is 90lbs. However, a physical trainer believes that the average weight might be higher. A random sample of 5 dumbbells with an average weight of 110lbs and a standard deviation of 18lbs. Using hypothesis testing check if the physical trainer's claim can be supported for a 95% confidence level. Solution: As the sample size is lesser than 30, the t-test is used. \(H_{0}\): \(\mu\) = 90, \(H_{1}\): \(\mu\) > 90 \(\overline{x}\) = 110, \(\mu\) = 90, n = 5, s = 18. \(\alpha\) = 0.05 Using the t-distribution table, the critical value is 2.132 t = \(\frac{\overline{x}-\mu}{\frac{s}{\sqrt{n}}}\) t = 2.484 As 2.484 > 2.132, the null hypothesis is rejected. Answer: The average weight of the dumbbells may be greater than 90lbs
• Example 2: The average score on a test is 80 with a standard deviation of 10. With a new teaching curriculum introduced it is believed that this score will change. On random testing, the score of 38 students, the mean was found to be 88. With a 0.05 significance level, is there any evidence to support this claim? Solution: This is an example of two-tail hypothesis testing. The z test will be used. \(H_{0}\): \(\mu\) = 80, \(H_{1}\): \(\mu\) ≠ 80 \(\overline{x}\) = 88, \(\mu\) = 80, n = 36, \(\sigma\) = 10. \(\alpha\) = 0.05 / 2 = 0.025 The critical value using the normal distribution table is 1.96 z = \(\frac{\overline{x}-\mu}{\frac{\sigma}{\sqrt{n}}}\) z = \(\frac{88-80}{\frac{10}{\sqrt{36}}}\) = 4.8 As 4.8 > 1.96, the null hypothesis is rejected. Answer: There is a difference in the scores after the new curriculum was introduced.
• Example 3: The average score of a class is 90. However, a teacher believes that the average score might be lower. The scores of 6 students were randomly measured. The mean was 82 with a standard deviation of 18. With a 0.05 significance level use hypothesis testing to check if this claim is true. Solution: The t test will be used. \(H_{0}\): \(\mu\) = 90, \(H_{1}\): \(\mu\) < 90 \(\overline{x}\) = 110, \(\mu\) = 90, n = 6, s = 18 The critical value from the t table is -2.015 t = \(\frac{\overline{x}-\mu}{\frac{s}{\sqrt{n}}}\) t = \(\frac{82-90}{\frac{18}{\sqrt{6}}}\) t = -1.088 As -1.088 > -2.015, we fail to reject the null hypothesis. Answer: There is not enough evidence to support the claim.

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FAQs on Hypothesis Testing

What is hypothesis testing.

Hypothesis testing in statistics is a tool that is used to make inferences about the population data. It is also used to check if the results of an experiment are valid.

What is the z Test in Hypothesis Testing?

The z test in hypothesis testing is used to find the z test statistic for normally distributed data . The z test is used when the standard deviation of the population is known and the sample size is greater than or equal to 30.

What is the t Test in Hypothesis Testing?

The t test in hypothesis testing is used when the data follows a student t distribution . It is used when the sample size is less than 30 and standard deviation of the population is not known.

What is the formula for z test in Hypothesis Testing?

The formula for a one sample z test in hypothesis testing is z = \(\frac{\overline{x}-\mu}{\frac{\sigma}{\sqrt{n}}}\) and for two samples is z = \(\frac{(\overline{x_{1}}-\overline{x_{2}})-(\mu_{1}-\mu_{2})}{\sqrt{\frac{\sigma_{1}^{2}}{n_{1}}+\frac{\sigma_{2}^{2}}{n_{2}}}}\).

What is the p Value in Hypothesis Testing?

The p value helps to determine if the test results are statistically significant or not. In hypothesis testing, the null hypothesis can either be rejected or not rejected based on the comparison between the p value and the alpha level.

What is One Tail Hypothesis Testing?

When the rejection region is only on one side of the distribution curve then it is known as one tail hypothesis testing. The right tail test and the left tail test are two types of directional hypothesis testing.

What is the Alpha Level in Two Tail Hypothesis Testing?

To get the alpha level in a two tail hypothesis testing divide \(\alpha\) by 2. This is done as there are two rejection regions in the curve.

Hypothesis Testing (cont...)

Hypothesis testing, the null and alternative hypothesis.

In order to undertake hypothesis testing you need to express your research hypothesis as a null and alternative hypothesis. The null hypothesis and alternative hypothesis are statements regarding the differences or effects that occur in the population. You will use your sample to test which statement (i.e., the null hypothesis or alternative hypothesis) is most likely (although technically, you test the evidence against the null hypothesis). So, with respect to our teaching example, the null and alternative hypothesis will reflect statements about all statistics students on graduate management courses.

The null hypothesis is essentially the "devil's advocate" position. That is, it assumes that whatever you are trying to prove did not happen ( hint: it usually states that something equals zero). For example, the two different teaching methods did not result in different exam performances (i.e., zero difference). Another example might be that there is no relationship between anxiety and athletic performance (i.e., the slope is zero). The alternative hypothesis states the opposite and is usually the hypothesis you are trying to prove (e.g., the two different teaching methods did result in different exam performances). Initially, you can state these hypotheses in more general terms (e.g., using terms like "effect", "relationship", etc.), as shown below for the teaching methods example:

Depending on how you want to "summarize" the exam performances will determine how you might want to write a more specific null and alternative hypothesis. For example, you could compare the mean exam performance of each group (i.e., the "seminar" group and the "lectures-only" group). This is what we will demonstrate here, but other options include comparing the distributions , medians , amongst other things. As such, we can state:

Now that you have identified the null and alternative hypotheses, you need to find evidence and develop a strategy for declaring your "support" for either the null or alternative hypothesis. We can do this using some statistical theory and some arbitrary cut-off points. Both these issues are dealt with next.

Significance levels

The level of statistical significance is often expressed as the so-called p -value . Depending on the statistical test you have chosen, you will calculate a probability (i.e., the p -value) of observing your sample results (or more extreme) given that the null hypothesis is true . Another way of phrasing this is to consider the probability that a difference in a mean score (or other statistic) could have arisen based on the assumption that there really is no difference. Let us consider this statement with respect to our example where we are interested in the difference in mean exam performance between two different teaching methods. If there really is no difference between the two teaching methods in the population (i.e., given that the null hypothesis is true), how likely would it be to see a difference in the mean exam performance between the two teaching methods as large as (or larger than) that which has been observed in your sample?

So, you might get a p -value such as 0.03 (i.e., p = .03). This means that there is a 3% chance of finding a difference as large as (or larger than) the one in your study given that the null hypothesis is true. However, you want to know whether this is "statistically significant". Typically, if there was a 5% or less chance (5 times in 100 or less) that the difference in the mean exam performance between the two teaching methods (or whatever statistic you are using) is as different as observed given the null hypothesis is true, you would reject the null hypothesis and accept the alternative hypothesis. Alternately, if the chance was greater than 5% (5 times in 100 or more), you would fail to reject the null hypothesis and would not accept the alternative hypothesis. As such, in this example where p = .03, we would reject the null hypothesis and accept the alternative hypothesis. We reject it because at a significance level of 0.03 (i.e., less than a 5% chance), the result we obtained could happen too frequently for us to be confident that it was the two teaching methods that had an effect on exam performance.

Whilst there is relatively little justification why a significance level of 0.05 is used rather than 0.01 or 0.10, for example, it is widely used in academic research. However, if you want to be particularly confident in your results, you can set a more stringent level of 0.01 (a 1% chance or less; 1 in 100 chance or less).

One- and two-tailed predictions

When considering whether we reject the null hypothesis and accept the alternative hypothesis, we need to consider the direction of the alternative hypothesis statement. For example, the alternative hypothesis that was stated earlier is:

The alternative hypothesis tells us two things. First, what predictions did we make about the effect of the independent variable(s) on the dependent variable(s)? Second, what was the predicted direction of this effect? Let's use our example to highlight these two points.

Sarah predicted that her teaching method (independent variable: teaching method), whereby she not only required her students to attend lectures, but also seminars, would have a positive effect (that is, increased) students' performance (dependent variable: exam marks). If an alternative hypothesis has a direction (and this is how you want to test it), the hypothesis is one-tailed. That is, it predicts direction of the effect. If the alternative hypothesis has stated that the effect was expected to be negative, this is also a one-tailed hypothesis.

Alternatively, a two-tailed prediction means that we do not make a choice over the direction that the effect of the experiment takes. Rather, it simply implies that the effect could be negative or positive. If Sarah had made a two-tailed prediction, the alternative hypothesis might have been:

In other words, we simply take out the word "positive", which implies the direction of our effect. In our example, making a two-tailed prediction may seem strange. After all, it would be logical to expect that "extra" tuition (going to seminar classes as well as lectures) would either have a positive effect on students' performance or no effect at all, but certainly not a negative effect. However, this is just our opinion (and hope) and certainly does not mean that we will get the effect we expect. Generally speaking, making a one-tail prediction (i.e., and testing for it this way) is frowned upon as it usually reflects the hope of a researcher rather than any certainty that it will happen. Notable exceptions to this rule are when there is only one possible way in which a change could occur. This can happen, for example, when biological activity/presence in measured. That is, a protein might be "dormant" and the stimulus you are using can only possibly "wake it up" (i.e., it cannot possibly reduce the activity of a "dormant" protein). In addition, for some statistical tests, one-tailed tests are not possible.

Rejecting or failing to reject the null hypothesis

Let's return finally to the question of whether we reject or fail to reject the null hypothesis.

If our statistical analysis shows that the significance level is below the cut-off value we have set (e.g., either 0.05 or 0.01), we reject the null hypothesis and accept the alternative hypothesis. Alternatively, if the significance level is above the cut-off value, we fail to reject the null hypothesis and cannot accept the alternative hypothesis. You should note that you cannot accept the null hypothesis, but only find evidence against it.

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S.3 hypothesis testing.

In reviewing hypothesis tests, we start first with the general idea. Then, we keep returning to the basic procedures of hypothesis testing, each time adding a little more detail.

The general idea of hypothesis testing involves:

• Making an initial assumption.
• Collecting evidence (data).
• Based on the available evidence (data), deciding whether to reject or not reject the initial assumption.

Every hypothesis test — regardless of the population parameter involved — requires the above three steps.

Example S.3.1

Is normal body temperature really 98.6 degrees f section  .

Consider the population of many, many adults. A researcher hypothesized that the average adult body temperature is lower than the often-advertised 98.6 degrees F. That is, the researcher wants an answer to the question: "Is the average adult body temperature 98.6 degrees? Or is it lower?" To answer his research question, the researcher starts by assuming that the average adult body temperature was 98.6 degrees F.

Then, the researcher went out and tried to find evidence that refutes his initial assumption. In doing so, he selects a random sample of 130 adults. The average body temperature of the 130 sampled adults is 98.25 degrees.

Then, the researcher uses the data he collected to make a decision about his initial assumption. It is either likely or unlikely that the researcher would collect the evidence he did given his initial assumption that the average adult body temperature is 98.6 degrees:

• If it is likely , then the researcher does not reject his initial assumption that the average adult body temperature is 98.6 degrees. There is not enough evidence to do otherwise.
• either the researcher's initial assumption is correct and he experienced a very unusual event;
• or the researcher's initial assumption is incorrect.

In statistics, we generally don't make claims that require us to believe that a very unusual event happened. That is, in the practice of statistics, if the evidence (data) we collected is unlikely in light of the initial assumption, then we reject our initial assumption.

Example S.3.2

Criminal trial analogy section  .

One place where you can consistently see the general idea of hypothesis testing in action is in criminal trials held in the United States. Our criminal justice system assumes "the defendant is innocent until proven guilty." That is, our initial assumption is that the defendant is innocent.

In the practice of statistics, we make our initial assumption when we state our two competing hypotheses -- the null hypothesis ( H 0 ) and the alternative hypothesis ( H A ). Here, our hypotheses are:

• H 0 : Defendant is not guilty (innocent)
• H A : Defendant is guilty

In statistics, we always assume the null hypothesis is true . That is, the null hypothesis is always our initial assumption.

The prosecution team then collects evidence — such as finger prints, blood spots, hair samples, carpet fibers, shoe prints, ransom notes, and handwriting samples — with the hopes of finding "sufficient evidence" to make the assumption of innocence refutable.

In statistics, the data are the evidence.

The jury then makes a decision based on the available evidence:

• If the jury finds sufficient evidence — beyond a reasonable doubt — to make the assumption of innocence refutable, the jury rejects the null hypothesis and deems the defendant guilty. We behave as if the defendant is guilty.
• If there is insufficient evidence, then the jury does not reject the null hypothesis . We behave as if the defendant is innocent.

In statistics, we always make one of two decisions. We either "reject the null hypothesis" or we "fail to reject the null hypothesis."

Errors in Hypothesis Testing Section  

Did you notice the use of the phrase "behave as if" in the previous discussion? We "behave as if" the defendant is guilty; we do not "prove" that the defendant is guilty. And, we "behave as if" the defendant is innocent; we do not "prove" that the defendant is innocent.

This is a very important distinction! We make our decision based on evidence not on 100% guaranteed proof. Again:

• If we reject the null hypothesis, we do not prove that the alternative hypothesis is true.
• If we do not reject the null hypothesis, we do not prove that the null hypothesis is true.

We merely state that there is enough evidence to behave one way or the other. This is always true in statistics! Because of this, whatever the decision, there is always a chance that we made an error .

Let's review the two types of errors that can be made in criminal trials:

Table S.3.2 shows how this corresponds to the two types of errors in hypothesis testing.

Note that, in statistics, we call the two types of errors by two different  names -- one is called a "Type I error," and the other is called  a "Type II error." Here are the formal definitions of the two types of errors:

There is always a chance of making one of these errors. But, a good scientific study will minimize the chance of doing so!

Making the Decision Section  

Recall that it is either likely or unlikely that we would observe the evidence we did given our initial assumption. If it is likely , we do not reject the null hypothesis. If it is unlikely , then we reject the null hypothesis in favor of the alternative hypothesis. Effectively, then, making the decision reduces to determining "likely" or "unlikely."

In statistics, there are two ways to determine whether the evidence is likely or unlikely given the initial assumption:

• We could take the " critical value approach " (favored in many of the older textbooks).
• Or, we could take the " P -value approach " (what is used most often in research, journal articles, and statistical software).

In the next two sections, we review the procedures behind each of these two approaches. To make our review concrete, let's imagine that μ is the average grade point average of all American students who major in mathematics. We first review the critical value approach for conducting each of the following three hypothesis tests about the population mean $\mu$:

In Practice

• We would want to conduct the first hypothesis test if we were interested in concluding that the average grade point average of the group is more than 3.
• We would want to conduct the second hypothesis test if we were interested in concluding that the average grade point average of the group is less than 3.
• And, we would want to conduct the third hypothesis test if we were only interested in concluding that the average grade point average of the group differs from 3 (without caring whether it is more or less than 3).

Upon completing the review of the critical value approach, we review the P -value approach for conducting each of the above three hypothesis tests about the population mean \(\mu\). The procedures that we review here for both approaches easily extend to hypothesis tests about any other population parameter.

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Introduction to Hypothesis Testing

A statistical hypothesis is an assumption about a population parameter .

For example, we may assume that the mean height of a male in the U.S. is 70 inches.

The assumption about the height is the statistical hypothesis and the true mean height of a male in the U.S. is the population parameter .

A hypothesis test is a formal statistical test we use to reject or fail to reject a statistical hypothesis.

The Two Types of Statistical Hypotheses

To test whether a statistical hypothesis about a population parameter is true, we obtain a random sample from the population and perform a hypothesis test on the sample data.

There are two types of statistical hypotheses:

The null hypothesis , denoted as H 0 , is the hypothesis that the sample data occurs purely from chance.

The alternative hypothesis , denoted as H 1 or H a , is the hypothesis that the sample data is influenced by some non-random cause.

Hypothesis Tests

A hypothesis test consists of five steps:

1. State the hypotheses. 

State the null and alternative hypotheses. These two hypotheses need to be mutually exclusive, so if one is true then the other must be false.

2. Determine a significance level to use for the hypothesis.

Decide on a significance level. Common choices are .01, .05, and .1. 

3. Find the test statistic.

Find the test statistic and the corresponding p-value. Often we are analyzing a population mean or proportion and the general formula to find the test statistic is: (sample statistic – population parameter) / (standard deviation of statistic)

4. Reject or fail to reject the null hypothesis.

Using the test statistic or the p-value, determine if you can reject or fail to reject the null hypothesis based on the significance level.

The p-value  tells us the strength of evidence in support of a null hypothesis. If the p-value is less than the significance level, we reject the null hypothesis.

5. Interpret the results. 

Interpret the results of the hypothesis test in the context of the question being asked. 

The Two Types of Decision Errors

There are two types of decision errors that one can make when doing a hypothesis test:

Type I error: You reject the null hypothesis when it is actually true. The probability of committing a Type I error is equal to the significance level, often called  alpha , and denoted as α.

Type II error: You fail to reject the null hypothesis when it is actually false. The probability of committing a Type II error is called the Power of the test or  Beta , denoted as β.

One-Tailed and Two-Tailed Tests

A statistical hypothesis can be one-tailed or two-tailed.

A one-tailed hypothesis involves making a “greater than” or “less than ” statement.

For example, suppose we assume the mean height of a male in the U.S. is greater than or equal to 70 inches. The null hypothesis would be H0: µ ≥ 70 inches and the alternative hypothesis would be Ha: µ < 70 inches.

A two-tailed hypothesis involves making an “equal to” or “not equal to” statement.

For example, suppose we assume the mean height of a male in the U.S. is equal to 70 inches. The null hypothesis would be H0: µ = 70 inches and the alternative hypothesis would be Ha: µ ≠ 70 inches.

Note: The “equal” sign is always included in the null hypothesis, whether it is =, ≥, or ≤.

Related:   What is a Directional Hypothesis?

Types of Hypothesis Tests

There are many different types of hypothesis tests you can perform depending on the type of data you’re working with and the goal of your analysis.

The following tutorials provide an explanation of the most common types of hypothesis tests:

Introduction to the One Sample t-test Introduction to the Two Sample t-test Introduction to the Paired Samples t-test Introduction to the One Proportion Z-Test Introduction to the Two Proportion Z-Test

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In today’s data-driven world , decisions are based on data all the time. Hypothesis plays a crucial role in that process, whether it may be making business decisions, in the health sector, academia, or in quality improvement. Without hypothesis & hypothesis tests, you risk drawing the wrong conclusions and making bad decisions. In this tutorial, you will look at Hypothesis Testing in Statistics.

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What Is Hypothesis Testing in Statistics?

Hypothesis Testing is a type of statistical analysis in which you put your assumptions about a population parameter to the test. It is used to estimate the relationship between 2 statistical variables.

Let's discuss few examples of statistical hypothesis from real-life - 

• A teacher assumes that 60% of his college's students come from lower-middle-class families.
• A doctor believes that 3D (Diet, Dose, and Discipline) is 90% effective for diabetic patients.

Now that you know about hypothesis testing, look at the two types of hypothesis testing in statistics.

Hypothesis Testing Formula

Z = ( x̅ – μ0 ) / (σ /√n)

• Here, x̅ is the sample mean,
• μ0 is the population mean,
• σ is the standard deviation,
• n is the sample size.

How Hypothesis Testing Works?

An analyst performs hypothesis testing on a statistical sample to present evidence of the plausibility of the null hypothesis. Measurements and analyses are conducted on a random sample of the population to test a theory. Analysts use a random population sample to test two hypotheses: the null and alternative hypotheses.

The null hypothesis is typically an equality hypothesis between population parameters; for example, a null hypothesis may claim that the population means return equals zero. The alternate hypothesis is essentially the inverse of the null hypothesis (e.g., the population means the return is not equal to zero). As a result, they are mutually exclusive, and only one can be correct. One of the two possibilities, however, will always be correct.

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Null Hypothesis and Alternate Hypothesis

The Null Hypothesis is the assumption that the event will not occur. A null hypothesis has no bearing on the study's outcome unless it is rejected.

H0 is the symbol for it, and it is pronounced H-naught.

The Alternate Hypothesis is the logical opposite of the null hypothesis. The acceptance of the alternative hypothesis follows the rejection of the null hypothesis. H1 is the symbol for it.

Let's understand this with an example.

A sanitizer manufacturer claims that its product kills 95 percent of germs on average. 

To put this company's claim to the test, create a null and alternate hypothesis.

H0 (Null Hypothesis): Average = 95%.

Alternative Hypothesis (H1): The average is less than 95%.

Another straightforward example to understand this concept is determining whether or not a coin is fair and balanced. The null hypothesis states that the probability of a show of heads is equal to the likelihood of a show of tails. In contrast, the alternate theory states that the probability of a show of heads and tails would be very different.

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Hypothesis Testing Calculation With Examples

Let's consider a hypothesis test for the average height of women in the United States. Suppose our null hypothesis is that the average height is 5'4". We gather a sample of 100 women and determine that their average height is 5'5". The standard deviation of population is 2.

To calculate the z-score, we would use the following formula:

z = ( x̅ – μ0 ) / (σ /√n)

z = (5'5" - 5'4") / (2" / √100)

z = 0.5 / (0.045)

We will reject the null hypothesis as the z-score of 11.11 is very large and conclude that there is evidence to suggest that the average height of women in the US is greater than 5'4".

Steps of Hypothesis Testing

Hypothesis testing is a statistical method to determine if there is enough evidence in a sample of data to infer that a certain condition is true for the entire population. Here’s a breakdown of the typical steps involved in hypothesis testing:

Formulate Hypotheses

• Null Hypothesis (H0): This hypothesis states that there is no effect or difference, and it is the hypothesis you attempt to reject with your test.
• Alternative Hypothesis (H1 or Ha): This hypothesis is what you might believe to be true or hope to prove true. It is usually considered the opposite of the null hypothesis.

Choose the Significance Level (α)

The significance level, often denoted by alpha (α), is the probability of rejecting the null hypothesis when it is true. Common choices for α are 0.05 (5%), 0.01 (1%), and 0.10 (10%).

Select the Appropriate Test

Choose a statistical test based on the type of data and the hypothesis. Common tests include t-tests, chi-square tests, ANOVA, and regression analysis . The selection depends on data type, distribution, sample size, and whether the hypothesis is one-tailed or two-tailed.

Collect Data

Gather the data that will be analyzed in the test. This data should be representative of the population to infer conclusions accurately.

Calculate the Test Statistic

Based on the collected data and the chosen test, calculate a test statistic that reflects how much the observed data deviates from the null hypothesis.

Determine the p-value

The p-value is the probability of observing test results at least as extreme as the results observed, assuming the null hypothesis is correct. It helps determine the strength of the evidence against the null hypothesis.

Make a Decision

Compare the p-value to the chosen significance level:

• If the p-value ≤ α: Reject the null hypothesis, suggesting sufficient evidence in the data supports the alternative hypothesis.
• If the p-value > α: Do not reject the null hypothesis, suggesting insufficient evidence to support the alternative hypothesis.

Report the Results

Present the findings from the hypothesis test, including the test statistic, p-value, and the conclusion about the hypotheses.

Perform Post-hoc Analysis (if necessary)

Depending on the results and the study design, further analysis may be needed to explore the data more deeply or to address multiple comparisons if several hypotheses were tested simultaneously.

Types of Hypothesis Testing

To determine whether a discovery or relationship is statistically significant, hypothesis testing uses a z-test. It usually checks to see if two means are the same (the null hypothesis). Only when the population standard deviation is known and the sample size is 30 data points or more, can a z-test be applied.

A statistical test called a t-test is employed to compare the means of two groups. To determine whether two groups differ or if a procedure or treatment affects the population of interest, it is frequently used in hypothesis testing.

Chi-Square 

You utilize a Chi-square test for hypothesis testing concerning whether your data is as predicted. To determine if the expected and observed results are well-fitted, the Chi-square test analyzes the differences between categorical variables from a random sample. The test's fundamental premise is that the observed values in your data should be compared to the predicted values that would be present if the null hypothesis were true.

Hypothesis Testing and Confidence Intervals

Both confidence intervals and hypothesis tests are inferential techniques that depend on approximating the sample distribution. Data from a sample is used to estimate a population parameter using confidence intervals. Data from a sample is used in hypothesis testing to examine a given hypothesis. We must have a postulated parameter to conduct hypothesis testing.

Bootstrap distributions and randomization distributions are created using comparable simulation techniques. The observed sample statistic is the focal point of a bootstrap distribution, whereas the null hypothesis value is the focal point of a randomization distribution.

A variety of feasible population parameter estimates are included in confidence ranges. In this lesson, we created just two-tailed confidence intervals. There is a direct connection between these two-tail confidence intervals and these two-tail hypothesis tests. The results of a two-tailed hypothesis test and two-tailed confidence intervals typically provide the same results. In other words, a hypothesis test at the 0.05 level will virtually always fail to reject the null hypothesis if the 95% confidence interval contains the predicted value. A hypothesis test at the 0.05 level will nearly certainly reject the null hypothesis if the 95% confidence interval does not include the hypothesized parameter.

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Simple and Composite Hypothesis Testing

Depending on the population distribution, you can classify the statistical hypothesis into two types.

Simple Hypothesis: A simple hypothesis specifies an exact value for the parameter.

Composite Hypothesis: A composite hypothesis specifies a range of values.

A company is claiming that their average sales for this quarter are 1000 units. This is an example of a simple hypothesis.

Suppose the company claims that the sales are in the range of 900 to 1000 units. Then this is a case of a composite hypothesis.

One-Tailed and Two-Tailed Hypothesis Testing

The One-Tailed test, also called a directional test, considers a critical region of data that would result in the null hypothesis being rejected if the test sample falls into it, inevitably meaning the acceptance of the alternate hypothesis.

In a one-tailed test, the critical distribution area is one-sided, meaning the test sample is either greater or lesser than a specific value.

In two tails, the test sample is checked to be greater or less than a range of values in a Two-Tailed test, implying that the critical distribution area is two-sided.

If the sample falls within this range, the alternate hypothesis will be accepted, and the null hypothesis will be rejected.

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Right Tailed Hypothesis Testing

If the larger than (>) sign appears in your hypothesis statement, you are using a right-tailed test, also known as an upper test. Or, to put it another way, the disparity is to the right. For instance, you can contrast the battery life before and after a change in production. Your hypothesis statements can be the following if you want to know if the battery life is longer than the original (let's say 90 hours):

• The null hypothesis is (H0 <= 90) or less change.
• A possibility is that battery life has risen (H1) > 90.

The crucial point in this situation is that the alternate hypothesis (H1), not the null hypothesis, decides whether you get a right-tailed test.

Left Tailed Hypothesis Testing

Alternative hypotheses that assert the true value of a parameter is lower than the null hypothesis are tested with a left-tailed test; they are indicated by the asterisk "<".

Suppose H0: mean = 50 and H1: mean not equal to 50

According to the H1, the mean can be greater than or less than 50. This is an example of a Two-tailed test.

In a similar manner, if H0: mean >=50, then H1: mean <50

Here the mean is less than 50. It is called a One-tailed test.

Type 1 and Type 2 Error

A hypothesis test can result in two types of errors.

Type 1 Error: A Type-I error occurs when sample results reject the null hypothesis despite being true.

Type 2 Error: A Type-II error occurs when the null hypothesis is not rejected when it is false, unlike a Type-I error.

Suppose a teacher evaluates the examination paper to decide whether a student passes or fails.

H0: Student has passed

H1: Student has failed

Type I error will be the teacher failing the student [rejects H0] although the student scored the passing marks [H0 was true]. 

Type II error will be the case where the teacher passes the student [do not reject H0] although the student did not score the passing marks [H1 is true].

Level of Significance

The alpha value is a criterion for determining whether a test statistic is statistically significant. In a statistical test, Alpha represents an acceptable probability of a Type I error. Because alpha is a probability, it can be anywhere between 0 and 1. In practice, the most commonly used alpha values are 0.01, 0.05, and 0.1, which represent a 1%, 5%, and 10% chance of a Type I error, respectively (i.e. rejecting the null hypothesis when it is in fact correct).

A p-value is a metric that expresses the likelihood that an observed difference could have occurred by chance. As the p-value decreases the statistical significance of the observed difference increases. If the p-value is too low, you reject the null hypothesis.

Here you have taken an example in which you are trying to test whether the new advertising campaign has increased the product's sales. The p-value is the likelihood that the null hypothesis, which states that there is no change in the sales due to the new advertising campaign, is true. If the p-value is .30, then there is a 30% chance that there is no increase or decrease in the product's sales.  If the p-value is 0.03, then there is a 3% probability that there is no increase or decrease in the sales value due to the new advertising campaign. As you can see, the lower the p-value, the chances of the alternate hypothesis being true increases, which means that the new advertising campaign causes an increase or decrease in sales.

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Why Is Hypothesis Testing Important in Research Methodology?

Hypothesis testing is crucial in research methodology for several reasons:

• Provides evidence-based conclusions: It allows researchers to make objective conclusions based on empirical data, providing evidence to support or refute their research hypotheses.
• Supports decision-making: It helps make informed decisions, such as accepting or rejecting a new treatment, implementing policy changes, or adopting new practices.
• Adds rigor and validity: It adds scientific rigor to research using statistical methods to analyze data, ensuring that conclusions are based on sound statistical evidence.
• Contributes to the advancement of knowledge: By testing hypotheses, researchers contribute to the growth of knowledge in their respective fields by confirming existing theories or discovering new patterns and relationships.

When Did Hypothesis Testing Begin?

Hypothesis testing as a formalized process began in the early 20th century, primarily through the work of statisticians such as Ronald A. Fisher, Jerzy Neyman, and Egon Pearson. The development of hypothesis testing is closely tied to the evolution of statistical methods during this period.

• Ronald A. Fisher (1920s): Fisher was one of the key figures in developing the foundation for modern statistical science. In the 1920s, he introduced the concept of the null hypothesis in his book "Statistical Methods for Research Workers" (1925). Fisher also developed significance testing to examine the likelihood of observing the collected data if the null hypothesis were true. He introduced p-values to determine the significance of the observed results.
• Neyman-Pearson Framework (1930s): Jerzy Neyman and Egon Pearson built on Fisher’s work and formalized the process of hypothesis testing even further. In the 1930s, they introduced the concepts of Type I and Type II errors and developed a decision-making framework widely used in hypothesis testing today. Their approach emphasized the balance between these errors and introduced the concepts of the power of a test and the alternative hypothesis.

The dialogue between Fisher's and Neyman-Pearson's approaches shaped the methods and philosophy of statistical hypothesis testing used today. Fisher emphasized the evidential interpretation of the p-value. At the same time, Neyman and Pearson advocated for a decision-theoretical approach in which hypotheses are either accepted or rejected based on pre-determined significance levels and power considerations.

The application and methodology of hypothesis testing have since become a cornerstone of statistical analysis across various scientific disciplines, marking a significant statistical development.

Limitations of Hypothesis Testing

Hypothesis testing has some limitations that researchers should be aware of:

• It cannot prove or establish the truth: Hypothesis testing provides evidence to support or reject a hypothesis, but it cannot confirm the absolute truth of the research question.
• Results are sample-specific: Hypothesis testing is based on analyzing a sample from a population, and the conclusions drawn are specific to that particular sample.
• Possible errors: During hypothesis testing, there is a chance of committing type I error (rejecting a true null hypothesis) or type II error (failing to reject a false null hypothesis).
• Assumptions and requirements: Different tests have specific assumptions and requirements that must be met to accurately interpret results.

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After reading this tutorial, you would have a much better understanding of hypothesis testing, one of the most important concepts in the field of Data Science . The majority of hypotheses are based on speculation about observed behavior, natural phenomena, or established theories.

If you are interested in statistics of data science and skills needed for such a career, you ought to explore the Post Graduate Program in Data Science.

If you have any questions regarding this ‘Hypothesis Testing In Statistics’ tutorial, do share them in the comment section. Our subject matter expert will respond to your queries. Happy learning!

1. What is hypothesis testing in statistics with example?

Hypothesis testing is a statistical method used to determine if there is enough evidence in a sample data to draw conclusions about a population. It involves formulating two competing hypotheses, the null hypothesis (H0) and the alternative hypothesis (Ha), and then collecting data to assess the evidence. An example: testing if a new drug improves patient recovery (Ha) compared to the standard treatment (H0) based on collected patient data.

2. What is H0 and H1 in statistics?

In statistics, H0​ and H1​ represent the null and alternative hypotheses. The null hypothesis, H0​, is the default assumption that no effect or difference exists between groups or conditions. The alternative hypothesis, H1​, is the competing claim suggesting an effect or a difference. Statistical tests determine whether to reject the null hypothesis in favor of the alternative hypothesis based on the data.

3. What is a simple hypothesis with an example?

A simple hypothesis is a specific statement predicting a single relationship between two variables. It posits a direct and uncomplicated outcome. For example, a simple hypothesis might state, "Increased sunlight exposure increases the growth rate of sunflowers." Here, the hypothesis suggests a direct relationship between the amount of sunlight (independent variable) and the growth rate of sunflowers (dependent variable), with no additional variables considered.

4. What are the 2 types of hypothesis testing?

• One-tailed (or one-sided) test: Tests for the significance of an effect in only one direction, either positive or negative.
• Two-tailed (or two-sided) test: Tests for the significance of an effect in both directions, allowing for the possibility of a positive or negative effect.

The choice between one-tailed and two-tailed tests depends on the specific research question and the directionality of the expected effect.

5. What are the 3 major types of hypothesis?

The three major types of hypotheses are:

• Null Hypothesis (H0): Represents the default assumption, stating that there is no significant effect or relationship in the data.
• Alternative Hypothesis (Ha): Contradicts the null hypothesis and proposes a specific effect or relationship that researchers want to investigate.
• Nondirectional Hypothesis: An alternative hypothesis that doesn't specify the direction of the effect, leaving it open for both positive and negative possibilities.

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About the Author

Avijeet is a Senior Research Analyst at Simplilearn. Passionate about Data Analytics, Machine Learning, and Deep Learning, Avijeet is also interested in politics, cricket, and football.

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What Is Hypothesis Testing?

• How It Works

4 Step Process

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• Fundamental Analysis

Hypothesis Testing: 4 Steps and Example

Hypothesis testing, sometimes called significance testing, is an act in statistics whereby an analyst tests an assumption regarding a population parameter. The methodology employed by the analyst depends on the nature of the data used and the reason for the analysis.

Hypothesis testing is used to assess the plausibility of a hypothesis by using sample data. Such data may come from a larger population or a data-generating process. The word "population" will be used for both of these cases in the following descriptions.

Key Takeaways

• Hypothesis testing is used to assess the plausibility of a hypothesis by using sample data.
• The test provides evidence concerning the plausibility of the hypothesis, given the data.
• Statistical analysts test a hypothesis by measuring and examining a random sample of the population being analyzed.
• The four steps of hypothesis testing include stating the hypotheses, formulating an analysis plan, analyzing the sample data, and analyzing the result.

How Hypothesis Testing Works

In hypothesis testing, an  analyst  tests a statistical sample, intending to provide evidence on the plausibility of the null hypothesis. Statistical analysts measure and examine a random sample of the population being analyzed. All analysts use a random population sample to test two different hypotheses: the null hypothesis and the alternative hypothesis.

The null hypothesis is usually a hypothesis of equality between population parameters; e.g., a null hypothesis may state that the population mean return is equal to zero. The alternative hypothesis is effectively the opposite of a null hypothesis. Thus, they are mutually exclusive , and only one can be true. However, one of the two hypotheses will always be true.

The null hypothesis is a statement about a population parameter, such as the population mean, that is assumed to be true.

• State the hypotheses.
• Formulate an analysis plan, which outlines how the data will be evaluated.
• Carry out the plan and analyze the sample data.
• Analyze the results and either reject the null hypothesis, or state that the null hypothesis is plausible, given the data.

Example of Hypothesis Testing

If an individual wants to test that a penny has exactly a 50% chance of landing on heads, the null hypothesis would be that 50% is correct, and the alternative hypothesis would be that 50% is not correct. Mathematically, the null hypothesis is represented as Ho: P = 0.5. The alternative hypothesis is shown as "Ha" and is identical to the null hypothesis, except with the equal sign struck-through, meaning that it does not equal 50%.

A random sample of 100 coin flips is taken, and the null hypothesis is tested. If it is found that the 100 coin flips were distributed as 40 heads and 60 tails, the analyst would assume that a penny does not have a 50% chance of landing on heads and would reject the null hypothesis and accept the alternative hypothesis.

If there were 48 heads and 52 tails, then it is plausible that the coin could be fair and still produce such a result. In cases such as this where the null hypothesis is "accepted," the analyst states that the difference between the expected results (50 heads and 50 tails) and the observed results (48 heads and 52 tails) is "explainable by chance alone."

When Did Hypothesis Testing Begin?

Some statisticians attribute the first hypothesis tests to satirical writer John Arbuthnot in 1710, who studied male and female births in England after observing that in nearly every year, male births exceeded female births by a slight proportion. Arbuthnot calculated that the probability of this happening by chance was small, and therefore it was due to “divine providence.”

What are the Benefits of Hypothesis Testing?

Hypothesis testing helps assess the accuracy of new ideas or theories by testing them against data. This allows researchers to determine whether the evidence supports their hypothesis, helping to avoid false claims and conclusions. Hypothesis testing also provides a framework for decision-making based on data rather than personal opinions or biases. By relying on statistical analysis, hypothesis testing helps to reduce the effects of chance and confounding variables, providing a robust framework for making informed conclusions.

What are the Limitations of Hypothesis Testing?

Hypothesis testing relies exclusively on data and doesn’t provide a comprehensive understanding of the subject being studied. Additionally, the accuracy of the results depends on the quality of the available data and the statistical methods used. Inaccurate data or inappropriate hypothesis formulation may lead to incorrect conclusions or failed tests. Hypothesis testing can also lead to errors, such as analysts either accepting or rejecting a null hypothesis when they shouldn’t have. These errors may result in false conclusions or missed opportunities to identify significant patterns or relationships in the data.

Hypothesis testing refers to a statistical process that helps researchers determine the reliability of a study. By using a well-formulated hypothesis and set of statistical tests, individuals or businesses can make inferences about the population that they are studying and draw conclusions based on the data presented. All hypothesis testing methods have the same four-step process, which includes stating the hypotheses, formulating an analysis plan, analyzing the sample data, and analyzing the result.

Sage. " Introduction to Hypothesis Testing ," Page 4.

Elder Research. " Who Invented the Null Hypothesis? "

Formplus. " Hypothesis Testing: Definition, Uses, Limitations and Examples ."

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• Hypothesis Testing: Definition, Uses, Limitations + Examples

Hypothesis testing is as old as the scientific method and is at the heart of the research process. 

Research exists to validate or disprove assumptions about various phenomena. The process of validation involves testing and it is in this context that we will explore hypothesis testing. 

What is a Hypothesis? 

A hypothesis is a calculated prediction or assumption about a population parameter based on limited evidence. The whole idea behind hypothesis formulation is testing—this means the researcher subjects his or her calculated assumption to a series of evaluations to know whether they are true or false. 

Typically, every research starts with a hypothesis—the investigator makes a claim and experiments to prove that this claim is true or false . For instance, if you predict that students who drink milk before class perform better than those who don’t, then this becomes a hypothesis that can be confirmed or refuted using an experiment.  

Read: What is Empirical Research Study? [Examples & Method]

What are the Types of Hypotheses? 

1. simple hypothesis.

Also known as a basic hypothesis, a simple hypothesis suggests that an independent variable is responsible for a corresponding dependent variable. In other words, an occurrence of the independent variable inevitably leads to an occurrence of the dependent variable. 

Typically, simple hypotheses are considered as generally true, and they establish a causal relationship between two variables. 

Examples of Simple Hypothesis  

• Drinking soda and other sugary drinks can cause obesity. 
• Smoking cigarettes daily leads to lung cancer.

2. Complex Hypothesis

A complex hypothesis is also known as a modal. It accounts for the causal relationship between two independent variables and the resulting dependent variables. This means that the combination of the independent variables leads to the occurrence of the dependent variables . 

Examples of Complex Hypotheses  

• Adults who do not smoke and drink are less likely to develop liver-related conditions.
• Global warming causes icebergs to melt which in turn causes major changes in weather patterns.

3. Null Hypothesis

As the name suggests, a null hypothesis is formed when a researcher suspects that there’s no relationship between the variables in an observation. In this case, the purpose of the research is to approve or disapprove this assumption. 

Examples of Null Hypothesis

• This is no significant change in a student’s performance if they drink coffee or tea before classes. 
• There’s no significant change in the growth of a plant if one uses distilled water only or vitamin-rich water. 

Read: Research Report: Definition, Types + [Writing Guide]

4. Alternative Hypothesis 

To disapprove a null hypothesis, the researcher has to come up with an opposite assumption—this assumption is known as the alternative hypothesis. This means if the null hypothesis says that A is false, the alternative hypothesis assumes that A is true. 

An alternative hypothesis can be directional or non-directional depending on the direction of the difference. A directional alternative hypothesis specifies the direction of the tested relationship, stating that one variable is predicted to be larger or smaller than the null value while a non-directional hypothesis only validates the existence of a difference without stating its direction. 

Examples of Alternative Hypotheses  

• Starting your day with a cup of tea instead of a cup of coffee can make you more alert in the morning. 
• The growth of a plant improves significantly when it receives distilled water instead of vitamin-rich water. 

5. Logical Hypothesis

Logical hypotheses are some of the most common types of calculated assumptions in systematic investigations. It is an attempt to use your reasoning to connect different pieces in research and build a theory using little evidence. In this case, the researcher uses any data available to him, to form a plausible assumption that can be tested. 

Examples of Logical Hypothesis

• Waking up early helps you to have a more productive day. 
• Beings from Mars would not be able to breathe the air in the atmosphere of the Earth. 

6. Empirical Hypothesis  

After forming a logical hypothesis, the next step is to create an empirical or working hypothesis. At this stage, your logical hypothesis undergoes systematic testing to prove or disprove the assumption. An empirical hypothesis is subject to several variables that can trigger changes and lead to specific outcomes. 

Examples of Empirical Testing 

• People who eat more fish run faster than people who eat meat.
• Women taking vitamin E grow hair faster than those taking vitamin K.

7. Statistical Hypothesis

When forming a statistical hypothesis, the researcher examines the portion of a population of interest and makes a calculated assumption based on the data from this sample. A statistical hypothesis is most common with systematic investigations involving a large target audience. Here, it’s impossible to collect responses from every member of the population so you have to depend on data from your sample and extrapolate the results to the wider population. 

Examples of Statistical Hypothesis  

• 45% of students in Louisiana have middle-income parents. 
• 80% of the UK’s population gets a divorce because of irreconcilable differences.

What is Hypothesis Testing? 

Hypothesis testing is an assessment method that allows researchers to determine the plausibility of a hypothesis. It involves testing an assumption about a specific population parameter to know whether it’s true or false. These population parameters include variance, standard deviation, and median. 

Typically, hypothesis testing starts with developing a null hypothesis and then performing several tests that support or reject the null hypothesis. The researcher uses test statistics to compare the association or relationship between two or more variables. 

Explore: Research Bias: Definition, Types + Examples

Researchers also use hypothesis testing to calculate the coefficient of variation and determine if the regression relationship and the correlation coefficient are statistically significant.

How Hypothesis Testing Works

The basis of hypothesis testing is to examine and analyze the null hypothesis and alternative hypothesis to know which one is the most plausible assumption. Since both assumptions are mutually exclusive, only one can be true. In other words, the occurrence of a null hypothesis destroys the chances of the alternative coming to life, and vice-versa. 

Interesting: 21 Chrome Extensions for Academic Researchers in 2021

What Are The Stages of Hypothesis Testing?  

To successfully confirm or refute an assumption, the researcher goes through five (5) stages of hypothesis testing; 

• Determine the null hypothesis
• Specify the alternative hypothesis
• Set the significance level
• Calculate the test statistics and corresponding P-value
• Draw your conclusion
• Determine the Null Hypothesis

Like we mentioned earlier, hypothesis testing starts with creating a null hypothesis which stands as an assumption that a certain statement is false or implausible. For example, the null hypothesis (H0) could suggest that different subgroups in the research population react to a variable in the same way. 

• Specify the Alternative Hypothesis

Once you know the variables for the null hypothesis, the next step is to determine the alternative hypothesis. The alternative hypothesis counters the null assumption by suggesting the statement or assertion is true. Depending on the purpose of your research, the alternative hypothesis can be one-sided or two-sided. 

Using the example we established earlier, the alternative hypothesis may argue that the different sub-groups react differently to the same variable based on several internal and external factors. 

• Set the Significance Level

Many researchers create a 5% allowance for accepting the value of an alternative hypothesis, even if the value is untrue. This means that there is a 0.05 chance that one would go with the value of the alternative hypothesis, despite the truth of the null hypothesis. 

Something to note here is that the smaller the significance level, the greater the burden of proof needed to reject the null hypothesis and support the alternative hypothesis.

Explore: What is Data Interpretation? + [Types, Method & Tools]

• Calculate the Test Statistics and Corresponding P-Value 

Test statistics in hypothesis testing allow you to compare different groups between variables while the p-value accounts for the probability of obtaining sample statistics if your null hypothesis is true. In this case, your test statistics can be the mean, median and similar parameters. 

If your p-value is 0.65, for example, then it means that the variable in your hypothesis will happen 65 in100 times by pure chance. Use this formula to determine the p-value for your data: 

• Draw Your Conclusions

After conducting a series of tests, you should be able to agree or refute the hypothesis based on feedback and insights from your sample data.  

Applications of Hypothesis Testing in Research

Hypothesis testing isn’t only confined to numbers and calculations; it also has several real-life applications in business, manufacturing, advertising, and medicine. 

In a factory or other manufacturing plants, hypothesis testing is an important part of quality and production control before the final products are approved and sent out to the consumer. 

During ideation and strategy development, C-level executives use hypothesis testing to evaluate their theories and assumptions before any form of implementation. For example, they could leverage hypothesis testing to determine whether or not some new advertising campaign, marketing technique, etc. causes increased sales. 

In addition, hypothesis testing is used during clinical trials to prove the efficacy of a drug or new medical method before its approval for widespread human usage. 

What is an Example of Hypothesis Testing?

An employer claims that her workers are of above-average intelligence. She takes a random sample of 20 of them and gets the following results: 

Mean IQ Scores: 110

Standard Deviation: 15 

Mean Population IQ: 100

Step 1: Using the value of the mean population IQ, we establish the null hypothesis as 100.

Step 2: State that the alternative hypothesis is greater than 100.

Step 3: State the alpha level as 0.05 or 5% 

Step 4: Find the rejection region area (given by your alpha level above) from the z-table. An area of .05 is equal to a z-score of 1.645.

Step 5: Calculate the test statistics using this formula

Z = (110–100) ÷ (15÷√20) 

10 ÷ 3.35 = 2.99 

If the value of the test statistics is higher than the value of the rejection region, then you should reject the null hypothesis. If it is less, then you cannot reject the null. 

In this case, 2.99 > 1.645 so we reject the null. 

Importance/Benefits of Hypothesis Testing 

The most significant benefit of hypothesis testing is it allows you to evaluate the strength of your claim or assumption before implementing it in your data set. Also, hypothesis testing is the only valid method to prove that something “is or is not”. Other benefits include: 

• Hypothesis testing provides a reliable framework for making any data decisions for your population of interest. 
• It helps the researcher to successfully extrapolate data from the sample to the larger population. 
• Hypothesis testing allows the researcher to determine whether the data from the sample is statistically significant. 
• Hypothesis testing is one of the most important processes for measuring the validity and reliability of outcomes in any systematic investigation. 
• It helps to provide links to the underlying theory and specific research questions.

Criticism and Limitations of Hypothesis Testing

Several limitations of hypothesis testing can affect the quality of data you get from this process. Some of these limitations include: 

• The interpretation of a p-value for observation depends on the stopping rule and definition of multiple comparisons. This makes it difficult to calculate since the stopping rule is subject to numerous interpretations, plus “multiple comparisons” are unavoidably ambiguous. 
• Conceptual issues often arise in hypothesis testing, especially if the researcher merges Fisher and Neyman-Pearson’s methods which are conceptually distinct. 
• In an attempt to focus on the statistical significance of the data, the researcher might ignore the estimation and confirmation by repeated experiments.
• Hypothesis testing can trigger publication bias, especially when it requires statistical significance as a criterion for publication.
• When used to detect whether a difference exists between groups, hypothesis testing can trigger absurd assumptions that affect the reliability of your observation.

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StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2024 Jan-.

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Hypothesis testing, p values, confidence intervals, and significance.

Jacob Shreffler ; Martin R. Huecker .

Affiliations

Last Update: March 13, 2023 .

• Definition/Introduction

Medical providers often rely on evidence-based medicine to guide decision-making in practice. Often a research hypothesis is tested with results provided, typically with p values, confidence intervals, or both. Additionally, statistical or research significance is estimated or determined by the investigators. Unfortunately, healthcare providers may have different comfort levels in interpreting these findings, which may affect the adequate application of the data.

• Issues of Concern

Without a foundational understanding of hypothesis testing, p values, confidence intervals, and the difference between statistical and clinical significance, it may affect healthcare providers' ability to make clinical decisions without relying purely on the research investigators deemed level of significance. Therefore, an overview of these concepts is provided to allow medical professionals to use their expertise to determine if results are reported sufficiently and if the study outcomes are clinically appropriate to be applied in healthcare practice.

Hypothesis Testing

Investigators conducting studies need research questions and hypotheses to guide analyses. Starting with broad research questions (RQs), investigators then identify a gap in current clinical practice or research. Any research problem or statement is grounded in a better understanding of relationships between two or more variables. For this article, we will use the following research question example:

Research Question: Is Drug 23 an effective treatment for Disease A?

Research questions do not directly imply specific guesses or predictions; we must formulate research hypotheses. A hypothesis is a predetermined declaration regarding the research question in which the investigator(s) makes a precise, educated guess about a study outcome. This is sometimes called the alternative hypothesis and ultimately allows the researcher to take a stance based on experience or insight from medical literature. An example of a hypothesis is below.

Research Hypothesis: Drug 23 will significantly reduce symptoms associated with Disease A compared to Drug 22.

The null hypothesis states that there is no statistical difference between groups based on the stated research hypothesis.

Researchers should be aware of journal recommendations when considering how to report p values, and manuscripts should remain internally consistent.

Regarding p values, as the number of individuals enrolled in a study (the sample size) increases, the likelihood of finding a statistically significant effect increases. With very large sample sizes, the p-value can be very low significant differences in the reduction of symptoms for Disease A between Drug 23 and Drug 22. The null hypothesis is deemed true until a study presents significant data to support rejecting the null hypothesis. Based on the results, the investigators will either reject the null hypothesis (if they found significant differences or associations) or fail to reject the null hypothesis (they could not provide proof that there were significant differences or associations).

To test a hypothesis, researchers obtain data on a representative sample to determine whether to reject or fail to reject a null hypothesis. In most research studies, it is not feasible to obtain data for an entire population. Using a sampling procedure allows for statistical inference, though this involves a certain possibility of error. [1]  When determining whether to reject or fail to reject the null hypothesis, mistakes can be made: Type I and Type II errors. Though it is impossible to ensure that these errors have not occurred, researchers should limit the possibilities of these faults. [2]

Significance

Significance is a term to describe the substantive importance of medical research. Statistical significance is the likelihood of results due to chance. [3]  Healthcare providers should always delineate statistical significance from clinical significance, a common error when reviewing biomedical research. [4]  When conceptualizing findings reported as either significant or not significant, healthcare providers should not simply accept researchers' results or conclusions without considering the clinical significance. Healthcare professionals should consider the clinical importance of findings and understand both p values and confidence intervals so they do not have to rely on the researchers to determine the level of significance. [5]  One criterion often used to determine statistical significance is the utilization of p values.

P values are used in research to determine whether the sample estimate is significantly different from a hypothesized value. The p-value is the probability that the observed effect within the study would have occurred by chance if, in reality, there was no true effect. Conventionally, data yielding a p<0.05 or p<0.01 is considered statistically significant. While some have debated that the 0.05 level should be lowered, it is still universally practiced. [6]  Hypothesis testing allows us to determine the size of the effect.

An example of findings reported with p values are below:

Statement: Drug 23 reduced patients' symptoms compared to Drug 22. Patients who received Drug 23 (n=100) were 2.1 times less likely than patients who received Drug 22 (n = 100) to experience symptoms of Disease A, p<0.05.

Statement:Individuals who were prescribed Drug 23 experienced fewer symptoms (M = 1.3, SD = 0.7) compared to individuals who were prescribed Drug 22 (M = 5.3, SD = 1.9). This finding was statistically significant, p= 0.02.

For either statement, if the threshold had been set at 0.05, the null hypothesis (that there was no relationship) should be rejected, and we should conclude significant differences. Noticeably, as can be seen in the two statements above, some researchers will report findings with < or > and others will provide an exact p-value (0.000001) but never zero [6] . When examining research, readers should understand how p values are reported. The best practice is to report all p values for all variables within a study design, rather than only providing p values for variables with significant findings. [7]  The inclusion of all p values provides evidence for study validity and limits suspicion for selective reporting/data mining.  

While researchers have historically used p values, experts who find p values problematic encourage the use of confidence intervals. [8] . P-values alone do not allow us to understand the size or the extent of the differences or associations. [3]  In March 2016, the American Statistical Association (ASA) released a statement on p values, noting that scientific decision-making and conclusions should not be based on a fixed p-value threshold (e.g., 0.05). They recommend focusing on the significance of results in the context of study design, quality of measurements, and validity of data. Ultimately, the ASA statement noted that in isolation, a p-value does not provide strong evidence. [9]

When conceptualizing clinical work, healthcare professionals should consider p values with a concurrent appraisal study design validity. For example, a p-value from a double-blinded randomized clinical trial (designed to minimize bias) should be weighted higher than one from a retrospective observational study [7] . The p-value debate has smoldered since the 1950s [10] , and replacement with confidence intervals has been suggested since the 1980s. [11]

Confidence Intervals

A confidence interval provides a range of values within given confidence (e.g., 95%), including the accurate value of the statistical constraint within a targeted population. [12]  Most research uses a 95% CI, but investigators can set any level (e.g., 90% CI, 99% CI). [13]  A CI provides a range with the lower bound and upper bound limits of a difference or association that would be plausible for a population. [14]  Therefore, a CI of 95% indicates that if a study were to be carried out 100 times, the range would contain the true value in 95, [15]  confidence intervals provide more evidence regarding the precision of an estimate compared to p-values. [6]

In consideration of the similar research example provided above, one could make the following statement with 95% CI:

Statement: Individuals who were prescribed Drug 23 had no symptoms after three days, which was significantly faster than those prescribed Drug 22; there was a mean difference between the two groups of days to the recovery of 4.2 days (95% CI: 1.9 – 7.8).

It is important to note that the width of the CI is affected by the standard error and the sample size; reducing a study sample number will result in less precision of the CI (increase the width). [14]  A larger width indicates a smaller sample size or a larger variability. [16]  A researcher would want to increase the precision of the CI. For example, a 95% CI of 1.43 – 1.47 is much more precise than the one provided in the example above. In research and clinical practice, CIs provide valuable information on whether the interval includes or excludes any clinically significant values. [14]

Null values are sometimes used for differences with CI (zero for differential comparisons and 1 for ratios). However, CIs provide more information than that. [15]  Consider this example: A hospital implements a new protocol that reduced wait time for patients in the emergency department by an average of 25 minutes (95% CI: -2.5 – 41 minutes). Because the range crosses zero, implementing this protocol in different populations could result in longer wait times; however, the range is much higher on the positive side. Thus, while the p-value used to detect statistical significance for this may result in "not significant" findings, individuals should examine this range, consider the study design, and weigh whether or not it is still worth piloting in their workplace.

Similarly to p-values, 95% CIs cannot control for researchers' errors (e.g., study bias or improper data analysis). [14]  In consideration of whether to report p-values or CIs, researchers should examine journal preferences. When in doubt, reporting both may be beneficial. [13]  An example is below:

Reporting both: Individuals who were prescribed Drug 23 had no symptoms after three days, which was significantly faster than those prescribed Drug 22, p = 0.009. There was a mean difference between the two groups of days to the recovery of 4.2 days (95% CI: 1.9 – 7.8).

• Clinical Significance

Recall that clinical significance and statistical significance are two different concepts. Healthcare providers should remember that a study with statistically significant differences and large sample size may be of no interest to clinicians, whereas a study with smaller sample size and statistically non-significant results could impact clinical practice. [14]  Additionally, as previously mentioned, a non-significant finding may reflect the study design itself rather than relationships between variables.

Healthcare providers using evidence-based medicine to inform practice should use clinical judgment to determine the practical importance of studies through careful evaluation of the design, sample size, power, likelihood of type I and type II errors, data analysis, and reporting of statistical findings (p values, 95% CI or both). [4]  Interestingly, some experts have called for "statistically significant" or "not significant" to be excluded from work as statistical significance never has and will never be equivalent to clinical significance. [17]

The decision on what is clinically significant can be challenging, depending on the providers' experience and especially the severity of the disease. Providers should use their knowledge and experiences to determine the meaningfulness of study results and make inferences based not only on significant or insignificant results by researchers but through their understanding of study limitations and practical implications.

• Nursing, Allied Health, and Interprofessional Team Interventions

All physicians, nurses, pharmacists, and other healthcare professionals should strive to understand the concepts in this chapter. These individuals should maintain the ability to review and incorporate new literature for evidence-based and safe care. 

• Review Questions
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• Comment on this article.

Disclosure: Jacob Shreffler declares no relevant financial relationships with ineligible companies.

Disclosure: Martin Huecker declares no relevant financial relationships with ineligible companies.

This book is distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) ( http://creativecommons.org/licenses/by-nc-nd/4.0/ ), which permits others to distribute the work, provided that the article is not altered or used commercially. You are not required to obtain permission to distribute this article, provided that you credit the author and journal.

• Cite this Page Shreffler J, Huecker MR. Hypothesis Testing, P Values, Confidence Intervals, and Significance. [Updated 2023 Mar 13]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2024 Jan-.

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Hypothesis Testing

About hypothesis testing.

Contents (Click to skip to the section):

What is a Hypothesis?

What is hypothesis testing.

• Hypothesis Testing Examples (One Sample Z Test).
• Hypothesis Test on a Mean (TI 83).

Bayesian Hypothesis Testing.

• More Hypothesis Testing Articles
• Hypothesis Tests in One Picture
• Critical Values

What is the Null Hypothesis?

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A hypothesis is an educated guess about something in the world around you. It should be testable, either by experiment or observation. For example:

• A new medicine you think might work.
• A way of teaching you think might be better.
• A possible location of new species.
• A fairer way to administer standardized tests.

It can really be anything at all as long as you can put it to the test.

What is a Hypothesis Statement?

If you are going to propose a hypothesis, it’s customary to write a statement. Your statement will look like this: “If I…(do this to an independent variable )….then (this will happen to the dependent variable ).” For example:

• If I (decrease the amount of water given to herbs) then (the herbs will increase in size).
• If I (give patients counseling in addition to medication) then (their overall depression scale will decrease).
• If I (give exams at noon instead of 7) then (student test scores will improve).
• If I (look in this certain location) then (I am more likely to find new species).

A good hypothesis statement should:

• Include an “if” and “then” statement (according to the University of California).
• Include both the independent and dependent variables.
• Be testable by experiment, survey or other scientifically sound technique.
• Be based on information in prior research (either yours or someone else’s).
• Have design criteria (for engineering or programming projects).

Hypothesis testing can be one of the most confusing aspects for students, mostly because before you can even perform a test, you have to know what your null hypothesis is. Often, those tricky word problems that you are faced with can be difficult to decipher. But it’s easier than you think; all you need to do is:

• Figure out your null hypothesis,
• State your null hypothesis,
• Choose what kind of test you need to perform,
• Either support or reject the null hypothesis .

If you trace back the history of science, the null hypothesis is always the accepted fact. Simple examples of null hypotheses that are generally accepted as being true are:

• DNA is shaped like a double helix.
• There are 8 planets in the solar system (excluding Pluto).
• Taking Vioxx can increase your risk of heart problems (a drug now taken off the market).

How do I State the Null Hypothesis?

You won’t be required to actually perform a real experiment or survey in elementary statistics (or even disprove a fact like “Pluto is a planet”!), so you’ll be given word problems from real-life situations. You’ll need to figure out what your hypothesis is from the problem. This can be a little trickier than just figuring out what the accepted fact is. With word problems, you are looking to find a fact that is nullifiable (i.e. something you can reject).

Hypothesis Testing Examples #1: Basic Example

A researcher thinks that if knee surgery patients go to physical therapy twice a week (instead of 3 times), their recovery period will be longer. Average recovery times for knee surgery patients is 8.2 weeks.

The hypothesis statement in this question is that the researcher believes the average recovery time is more than 8.2 weeks. It can be written in mathematical terms as: H 1 : μ > 8.2

Next, you’ll need to state the null hypothesis .  That’s what will happen if the researcher is wrong . In the above example, if the researcher is wrong then the recovery time is less than or equal to 8.2 weeks. In math, that’s: H 0 μ ≤ 8.2

Rejecting the null hypothesis

Ten or so years ago, we believed that there were 9 planets in the solar system. Pluto was demoted as a planet in 2006. The null hypothesis of “Pluto is a planet” was replaced by “Pluto is not a planet.” Of course, rejecting the null hypothesis isn’t always that easy— the hard part is usually figuring out what your null hypothesis is in the first place.

Hypothesis Testing Examples (One Sample Z Test)

The one sample z test isn’t used very often (because we rarely know the actual population standard deviation ). However, it’s a good idea to understand how it works as it’s one of the simplest tests you can perform in hypothesis testing. In English class you got to learn the basics (like grammar and spelling) before you could write a story; think of one sample z tests as the foundation for understanding more complex hypothesis testing. This page contains two hypothesis testing examples for one sample z-tests .

One Sample Hypothesis Testing Example: One Tailed Z Test

A principal at a certain school claims that the students in his school are above average intelligence. A random sample of thirty students IQ scores have a mean score of 112.5. Is there sufficient evidence to support the principal’s claim? The mean population IQ is 100 with a standard deviation of 15.

Step 1: State the Null hypothesis . The accepted fact is that the population mean is 100, so: H 0 : μ = 100.

Step 2: State the Alternate Hypothesis . The claim is that the students have above average IQ scores, so: H 1 : μ > 100. The fact that we are looking for scores “greater than” a certain point means that this is a one-tailed test.

Step 4: State the alpha level . If you aren’t given an alpha level , use 5% (0.05).

Step 5: Find the rejection region area (given by your alpha level above) from the z-table . An area of .05 is equal to a z-score of 1.645.

Step 6: If Step 6 is greater than Step 5, reject the null hypothesis. If it’s less than Step 5, you cannot reject the null hypothesis. In this case, it is more (4.56 > 1.645), so you can reject the null.

One Sample Hypothesis Testing Examples: #3

Blood glucose levels for obese patients have a mean of 100 with a standard deviation of 15. A researcher thinks that a diet high in raw cornstarch will have a positive or negative effect on blood glucose levels. A sample of 30 patients who have tried the raw cornstarch diet have a mean glucose level of 140. Test the hypothesis that the raw cornstarch had an effect.

• State the null hypothesis : H 0 :μ=100
• State the alternate hypothesis : H 1 :≠100
• State your alpha level. We’ll use 0.05 for this example. As this is a two-tailed test, split the alpha into two. 0.05/2=0.025
• Find the z-score associated with your alpha level . You’re looking for the area in one tail only . A z-score for 0.75(1-0.025=0.975) is 1.96. As this is a two-tailed test, you would also be considering the left tail (z = 1.96)
•   If Step 5 is less than -1.96 or greater than 1.96 (Step 3), reject the null hypothesis . In this case, it is greater, so you can reject the null.

*This process is made much easier if you use a TI-83 or Excel to calculate the z-score (the “critical value”). See:

• Critical z value TI 83
• Z Score in Excel

Hypothesis Testing Examples: Mean (Using TI 83)

You can use the TI 83 calculator for hypothesis testing, but the calculator won’t figure out the null and alternate hypotheses; that’s up to you to read the question and input it into the calculator.

Example problem : A sample of 200 people has a mean age of 21 with a population standard deviation (σ) of 5. Test the hypothesis that the population mean is 18.9 at α = 0.05.

Step 1: State the null hypothesis. In this case, the null hypothesis is that the population mean is 18.9, so we write: H 0 : μ = 18.9

Step 2: State the alternative hypothesis. We want to know if our sample, which has a mean of 21 instead of 18.9, really is different from the population, therefore our alternate hypothesis: H 1 : μ ≠ 18.9

Step 3: Press Stat then press the right arrow twice to select TESTS.

Step 4: Press 1 to select 1:Z-Test… . Press ENTER.

Step 5: Use the right arrow to select Stats .

Step 6: Enter the data from the problem: μ 0 : 18.9 σ: 5 x : 21 n: 200 μ: ≠μ 0

Step 7: Arrow down to Calculate and press ENTER. The calculator shows the p-value: p = 2.87 × 10 -9

This is smaller than our alpha value of .05. That means we should reject the null hypothesis .

Bayesian Hypothesis Testing: What is it?

Bayesian hypothesis testing helps to answer the question: Can the results from a test or survey be repeated? Why do we care if a test can be repeated? Let’s say twenty people in the same village came down with leukemia. A group of researchers find that cell-phone towers are to blame. However, a second study found that cell-phone towers had nothing to do with the cancer cluster in the village. In fact, they found that the cancers were completely random. If that sounds impossible, it actually can happen! Clusters of cancer can happen simply by chance . There could be many reasons why the first study was faulty. One of the main reasons could be that they just didn’t take into account that sometimes things happen randomly and we just don’t know why.

It’s good science to let people know if your study results are solid, or if they could have happened by chance. The usual way of doing this is to test your results with a p-value . A p value is a number that you get by running a hypothesis test on your data. A P value of 0.05 (5%) or less is usually enough to claim that your results are repeatable. However, there’s another way to test the validity of your results: Bayesian Hypothesis testing. This type of testing gives you another way to test the strength of your results.

Traditional testing (the type you probably came across in elementary stats or AP stats) is called Non-Bayesian. It is how often an outcome happens over repeated runs of the experiment. It’s an objective view of whether an experiment is repeatable. Bayesian hypothesis testing is a subjective view of the same thing. It takes into account how much faith you have in your results. In other words, would you wager money on the outcome of your experiment?

Differences Between Traditional and Bayesian Hypothesis Testing.

Traditional testing (Non Bayesian) requires you to repeat sampling over and over, while Bayesian testing does not. The main different between the two is in the first step of testing: stating a probability model. In Bayesian testing you add prior knowledge to this step. It also requires use of a posterior probability , which is the conditional probability given to a random event after all the evidence is considered.

Arguments for Bayesian Testing.

Many researchers think that it is a better alternative to traditional testing, because it:

• Includes prior knowledge about the data.
• Takes into account personal beliefs about the results.

Arguments against.

• Including prior data or knowledge isn’t justifiable.
• It is difficult to calculate compared to non-Bayesian testing.

Back to top

Hypothesis Testing Articles

• What is Ad Hoc Testing?
• Composite Hypothesis Test
• What is a Rejection Region?
• What is a Two Tailed Test?
• How to Decide if a Hypothesis Test is a One Tailed Test or a Two Tailed Test.
• How to Decide if a Hypothesis is a Left Tailed Test or a Right-Tailed Test.
• How to State the Null Hypothesis in Statistics.
• How to Find a Critical Value .
• How to Support or Reject a Null Hypothesis.

Specific Tests:

• Brunner Munzel Test (Generalized Wilcoxon Test).
• Chi Square Test for Normality.
• Cochran-Mantel-Haenszel Test.
• Granger Causality Test .
• Hotelling’s T-Squared.
• KPSS Test .
• What is a Likelihood-Ratio Test?
• Log rank test .
• MANCOVA Assumptions.
• MANCOVA Sample Size.
• Marascuilo Procedure
• Rao’s Spacing Test
• Rayleigh test of uniformity.
• Sequential Probability Ratio Test.
• How to Run a Sign Test.
• T Test: one sample.
• T-Test: Two sample .
• Welch’s ANOVA .
• Welch’s Test for Unequal Variances .
• Z-Test: one sample .
• Z Test: Two Proportion.
• Wald Test .

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• False Discovery Rate
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• The Multiple Testing Problem .
• What is the Neyman-Pearson Lemma?
• What is an Omnibus Test?
• One Sample Median Test .
• How to Find a Sample Size (General Instructions).
• Sig 2(Tailed) meaning in results
• What is a Standardized Test Statistic?
• How to Find Standard Error
• Standardized values: Example.
• How to Calculate a T-Score.
• T-Score Vs. a Z.Score.
• Testing a Single Mean.
• Unequal Sample Sizes.
• Uniformly Most Powerful Tests.
• How to Calculate a Z-Score.

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Hypothesis testing is a form of statistical inference that uses data from a sample to draw conclusions about a population parameter or a population probability distribution . First, a tentative assumption is made about the parameter or distribution. This assumption is called the null hypothesis and is denoted by H 0 . An alternative hypothesis (denoted H a ), which is the opposite of what is stated in the null hypothesis, is then defined. The hypothesis-testing procedure involves using sample data to determine whether or not H 0 can be rejected. If H 0 is rejected, the statistical conclusion is that the alternative hypothesis H a is true.

For example, assume that a radio station selects the music it plays based on the assumption that the average age of its listening audience is 30 years. To determine whether this assumption is valid, a hypothesis test could be conducted with the null hypothesis given as H 0 : μ = 30 and the alternative hypothesis given as H a : μ ≠ 30. Based on a sample of individuals from the listening audience, the sample mean age, x̄ , can be computed and used to determine whether there is sufficient statistical evidence to reject H 0 . Conceptually, a value of the sample mean that is “close” to 30 is consistent with the null hypothesis, while a value of the sample mean that is “not close” to 30 provides support for the alternative hypothesis. What is considered “close” and “not close” is determined by using the sampling distribution of x̄ .

Ideally, the hypothesis-testing procedure leads to the acceptance of H 0 when H 0 is true and the rejection of H 0 when H 0 is false. Unfortunately, since hypothesis tests are based on sample information, the possibility of errors must be considered. A type I error corresponds to rejecting H 0 when H 0 is actually true, and a type II error corresponds to accepting H 0 when H 0 is false. The probability of making a type I error is denoted by α, and the probability of making a type II error is denoted by β.

In using the hypothesis-testing procedure to determine if the null hypothesis should be rejected, the person conducting the hypothesis test specifies the maximum allowable probability of making a type I error, called the level of significance for the test. Common choices for the level of significance are α = 0.05 and α = 0.01. Although most applications of hypothesis testing control the probability of making a type I error, they do not always control the probability of making a type II error. A graph known as an operating-characteristic curve can be constructed to show how changes in the sample size affect the probability of making a type II error.

A concept known as the p -value provides a convenient basis for drawing conclusions in hypothesis-testing applications. The p -value is a measure of how likely the sample results are, assuming the null hypothesis is true; the smaller the p -value, the less likely the sample results. If the p -value is less than α, the null hypothesis can be rejected; otherwise, the null hypothesis cannot be rejected. The p -value is often called the observed level of significance for the test.

A hypothesis test can be performed on parameters of one or more populations as well as in a variety of other situations. In each instance, the process begins with the formulation of null and alternative hypotheses about the population. In addition to the population mean, hypothesis-testing procedures are available for population parameters such as proportions, variances , standard deviations , and medians .

Hypothesis tests are also conducted in regression and correlation analysis to determine if the regression relationship and the correlation coefficient are statistically significant (see below Regression and correlation analysis ). A goodness-of-fit test refers to a hypothesis test in which the null hypothesis is that the population has a specific probability distribution, such as a normal probability distribution. Nonparametric statistical methods also involve a variety of hypothesis-testing procedures.

The methods of statistical inference previously described are often referred to as classical methods. Bayesian methods (so called after the English mathematician Thomas Bayes ) provide alternatives that allow one to combine prior information about a population parameter with information contained in a sample to guide the statistical inference process. A prior probability distribution for a parameter of interest is specified first. Sample information is then obtained and combined through an application of Bayes’s theorem to provide a posterior probability distribution for the parameter. The posterior distribution provides the basis for statistical inferences concerning the parameter.

A key, and somewhat controversial, feature of Bayesian methods is the notion of a probability distribution for a population parameter. According to classical statistics, parameters are constants and cannot be represented as random variables. Bayesian proponents argue that, if a parameter value is unknown, then it makes sense to specify a probability distribution that describes the possible values for the parameter as well as their likelihood . The Bayesian approach permits the use of objective data or subjective opinion in specifying a prior distribution. With the Bayesian approach, different individuals might specify different prior distributions. Classical statisticians argue that for this reason Bayesian methods suffer from a lack of objectivity. Bayesian proponents argue that the classical methods of statistical inference have built-in subjectivity (through the choice of a sampling plan) and that the advantage of the Bayesian approach is that the subjectivity is made explicit.

Bayesian methods have been used extensively in statistical decision theory (see below Decision analysis ). In this context , Bayes’s theorem provides a mechanism for combining a prior probability distribution for the states of nature with sample information to provide a revised (posterior) probability distribution about the states of nature. These posterior probabilities are then used to make better decisions.

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Unit 12: Significance tests (hypothesis testing)

About this unit.

Significance tests give us a formal process for using sample data to evaluate the likelihood of some claim about a population value. Learn how to conduct significance tests and calculate p-values to see how likely a sample result is to occur by random chance. You'll also see how we use p-values to make conclusions about hypotheses.

The idea of significance tests

• Simple hypothesis testing (Opens a modal)
• Idea behind hypothesis testing (Opens a modal)
• Examples of null and alternative hypotheses (Opens a modal)
• P-values and significance tests (Opens a modal)
• Comparing P-values to different significance levels (Opens a modal)
• Estimating a P-value from a simulation (Opens a modal)
• Using P-values to make conclusions (Opens a modal)
• Simple hypothesis testing Get 3 of 4 questions to level up!
• Writing null and alternative hypotheses Get 3 of 4 questions to level up!
• Estimating P-values from simulations Get 3 of 4 questions to level up!

Error probabilities and power

• Introduction to Type I and Type II errors (Opens a modal)
• Type 1 errors (Opens a modal)
• Examples identifying Type I and Type II errors (Opens a modal)
• Introduction to power in significance tests (Opens a modal)
• Examples thinking about power in significance tests (Opens a modal)
• Consequences of errors and significance (Opens a modal)
• Type I vs Type II error Get 3 of 4 questions to level up!
• Error probabilities and power Get 3 of 4 questions to level up!

Tests about a population proportion

• Constructing hypotheses for a significance test about a proportion (Opens a modal)
• Conditions for a z test about a proportion (Opens a modal)
• Reference: Conditions for inference on a proportion (Opens a modal)
• Calculating a z statistic in a test about a proportion (Opens a modal)
• Calculating a P-value given a z statistic (Opens a modal)
• Making conclusions in a test about a proportion (Opens a modal)
• Writing hypotheses for a test about a proportion Get 3 of 4 questions to level up!
• Conditions for a z test about a proportion Get 3 of 4 questions to level up!
• Calculating the test statistic in a z test for a proportion Get 3 of 4 questions to level up!
• Calculating the P-value in a z test for a proportion Get 3 of 4 questions to level up!
• Making conclusions in a z test for a proportion Get 3 of 4 questions to level up!

Tests about a population mean

• Writing hypotheses for a significance test about a mean (Opens a modal)
• Conditions for a t test about a mean (Opens a modal)
• Reference: Conditions for inference on a mean (Opens a modal)
• When to use z or t statistics in significance tests (Opens a modal)
• Example calculating t statistic for a test about a mean (Opens a modal)
• Using TI calculator for P-value from t statistic (Opens a modal)
• Using a table to estimate P-value from t statistic (Opens a modal)
• Comparing P-value from t statistic to significance level (Opens a modal)
• Free response example: Significance test for a mean (Opens a modal)
• Writing hypotheses for a test about a mean Get 3 of 4 questions to level up!
• Conditions for a t test about a mean Get 3 of 4 questions to level up!
• Calculating the test statistic in a t test for a mean Get 3 of 4 questions to level up!
• Calculating the P-value in a t test for a mean Get 3 of 4 questions to level up!
• Making conclusions in a t test for a mean Get 3 of 4 questions to level up!

More significance testing videos

• Hypothesis testing and p-values (Opens a modal)
• One-tailed and two-tailed tests (Opens a modal)
• Z-statistics vs. T-statistics (Opens a modal)
• Small sample hypothesis test (Opens a modal)
• Large sample proportion hypothesis testing (Opens a modal)

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Hypothesis testing involves formulating assumptions about population parameters based on sample statistics and rigorously evaluating these assumptions against empirical evidence. This article sheds light on the significance of hypothesis testing and the critical steps involved in the process.

What is Hypothesis Testing?

Hypothesis testing is a statistical method that is used to make a statistical decision using experimental data. Hypothesis testing is basically an assumption that we make about a population parameter. It evaluates two mutually exclusive statements about a population to determine which statement is best supported by the sample data. 

Example: You say an average height in the class is 30 or a boy is taller than a girl. All of these is an assumption that we are assuming, and we need some statistical way to prove these. We need some mathematical conclusion whatever we are assuming is true.

Defining Hypotheses

Key Terms of Hypothesis Testing

• P-value: The P value , or calculated probability, is the probability of finding the observed/extreme results when the null hypothesis(H0) of a study-given problem is true. If your P-value is less than the chosen significance level then you reject the null hypothesis i.e. accept that your sample claims to support the alternative hypothesis.
• Test Statistic: The test statistic is a numerical value calculated from sample data during a hypothesis test, used to determine whether to reject the null hypothesis. It is compared to a critical value or p-value to make decisions about the statistical significance of the observed results.
• Critical value : The critical value in statistics is a threshold or cutoff point used to determine whether to reject the null hypothesis in a hypothesis test.
• Degrees of freedom: Degrees of freedom are associated with the variability or freedom one has in estimating a parameter. The degrees of freedom are related to the sample size and determine the shape.

Why do we use Hypothesis Testing?

Hypothesis testing is an important procedure in statistics. Hypothesis testing evaluates two mutually exclusive population statements to determine which statement is most supported by sample data. When we say that the findings are statistically significant, thanks to hypothesis testing. 

One-Tailed and Two-Tailed Test

One tailed test focuses on one direction, either greater than or less than a specified value. We use a one-tailed test when there is a clear directional expectation based on prior knowledge or theory. The critical region is located on only one side of the distribution curve. If the sample falls into this critical region, the null hypothesis is rejected in favor of the alternative hypothesis.

One-Tailed Test

There are two types of one-tailed test:

Two-Tailed Test

A two-tailed test considers both directions, greater than and less than a specified value.We use a two-tailed test when there is no specific directional expectation, and want to detect any significant difference.

What are Type 1 and Type 2 errors in Hypothesis Testing?

In hypothesis testing, Type I and Type II errors are two possible errors that researchers can make when drawing conclusions about a population based on a sample of data. These errors are associated with the decisions made regarding the null hypothesis and the alternative hypothesis.

How does Hypothesis Testing work?

Step 1: define null and alternative hypothesis.

We first identify the problem about which we want to make an assumption keeping in mind that our assumption should be contradictory to one another, assuming Normally distributed data.

Step 2 – Choose significance level

Step 3 – Collect and Analyze data.

Gather relevant data through observation or experimentation. Analyze the data using appropriate statistical methods to obtain a test statistic.

Step 4-Calculate Test Statistic

The data for the tests are evaluated in this step we look for various scores based on the characteristics of data. The choice of the test statistic depends on the type of hypothesis test being conducted.

There are various hypothesis tests, each appropriate for various goal to calculate our test. This could be a Z-test , Chi-square , T-test , and so on.

• Z-test : If population means and standard deviations are known. Z-statistic is commonly used.
• t-test : If population standard deviations are unknown. and sample size is small than t-test statistic is more appropriate.
• Chi-square test : Chi-square test is used for categorical data or for testing independence in contingency tables
• F-test : F-test is often used in analysis of variance (ANOVA) to compare variances or test the equality of means across multiple groups.

We have a smaller dataset, So, T-test is more appropriate to test our hypothesis.

T-statistic is a measure of the difference between the means of two groups relative to the variability within each group. It is calculated as the difference between the sample means divided by the standard error of the difference. It is also known as the t-value or t-score.

Step 5 – Comparing Test Statistic:

In this stage, we decide where we should accept the null hypothesis or reject the null hypothesis. There are two ways to decide where we should accept or reject the null hypothesis.

Method A: Using Crtical values

Comparing the test statistic and tabulated critical value we have,

• If Test Statistic>Critical Value: Reject the null hypothesis.
• If Test Statistic≤Critical Value: Fail to reject the null hypothesis.

Note: Critical values are predetermined threshold values that are used to make a decision in hypothesis testing. To determine critical values for hypothesis testing, we typically refer to a statistical distribution table , such as the normal distribution or t-distribution tables based on.

Method B: Using P-values

We can also come to an conclusion using the p-value,

Note : The p-value is the probability of obtaining a test statistic as extreme as, or more extreme than, the one observed in the sample, assuming the null hypothesis is true. To determine p-value for hypothesis testing, we typically refer to a statistical distribution table , such as the normal distribution or t-distribution tables based on.

Step 7- Interpret the Results

At last, we can conclude our experiment using method A or B.

Calculating test statistic

To validate our hypothesis about a population parameter we use statistical functions . We use the z-score, p-value, and level of significance(alpha) to make evidence for our hypothesis for normally distributed data .

1. Z-statistics:

When population means and standard deviations are known.

• μ represents the population mean, 
• σ is the standard deviation
• and n is the size of the sample.

2. T-Statistics

T test is used when n<30,

t-statistic calculation is given by:

• t = t-score,
• x̄ = sample mean
• μ = population mean,
• s = standard deviation of the sample,
• n = sample size

3. Chi-Square Test

Chi-Square Test for Independence categorical Data (Non-normally distributed) using:

• i,j are the rows and columns index respectively.

Real life Hypothesis Testing example

Let’s examine hypothesis testing using two real life situations,

Case A: D oes a New Drug Affect Blood Pressure?

Imagine a pharmaceutical company has developed a new drug that they believe can effectively lower blood pressure in patients with hypertension. Before bringing the drug to market, they need to conduct a study to assess its impact on blood pressure.

• Before Treatment: 120, 122, 118, 130, 125, 128, 115, 121, 123, 119
• After Treatment: 115, 120, 112, 128, 122, 125, 110, 117, 119, 114

Step 1 : Define the Hypothesis

• Null Hypothesis : (H 0 )The new drug has no effect on blood pressure.
• Alternate Hypothesis : (H 1 )The new drug has an effect on blood pressure.

Step 2: Define the Significance level

Let’s consider the Significance level at 0.05, indicating rejection of the null hypothesis.

If the evidence suggests less than a 5% chance of observing the results due to random variation.

Step 3 : Compute the test statistic

Using paired T-test analyze the data to obtain a test statistic and a p-value.

The test statistic (e.g., T-statistic) is calculated based on the differences between blood pressure measurements before and after treatment.

t = m/(s/√n)

• m  = mean of the difference i.e X after, X before
• s  = standard deviation of the difference (d) i.e d i ​= X after, i ​− X before,
• n  = sample size,

then, m= -3.9, s= 1.8 and n= 10

we, calculate the , T-statistic = -9 based on the formula for paired t test

Step 4: Find the p-value

The calculated t-statistic is -9 and degrees of freedom df = 9, you can find the p-value using statistical software or a t-distribution table.

thus, p-value = 8.538051223166285e-06

Step 5: Result

• If the p-value is less than or equal to 0.05, the researchers reject the null hypothesis.
• If the p-value is greater than 0.05, they fail to reject the null hypothesis.

Conclusion: Since the p-value (8.538051223166285e-06) is less than the significance level (0.05), the researchers reject the null hypothesis. There is statistically significant evidence that the average blood pressure before and after treatment with the new drug is different.

Python Implementation of Hypothesis Testing

Let’s create hypothesis testing with python, where we are testing whether a new drug affects blood pressure. For this example, we will use a paired T-test. We’ll use the scipy.stats library for the T-test.

Scipy is a mathematical library in Python that is mostly used for mathematical equations and computations.

We will implement our first real life problem via python,

In the above example, given the T-statistic of approximately -9 and an extremely small p-value, the results indicate a strong case to reject the null hypothesis at a significance level of 0.05. 

• The results suggest that the new drug, treatment, or intervention has a significant effect on lowering blood pressure.
• The negative T-statistic indicates that the mean blood pressure after treatment is significantly lower than the assumed population mean before treatment.

Case B : Cholesterol level in a population

Data: A sample of 25 individuals is taken, and their cholesterol levels are measured.

Cholesterol Levels (mg/dL): 205, 198, 210, 190, 215, 205, 200, 192, 198, 205, 198, 202, 208, 200, 205, 198, 205, 210, 192, 205, 198, 205, 210, 192, 205.

Populations Mean = 200

Population Standard Deviation (σ): 5 mg/dL(given for this problem)

Step 1: Define the Hypothesis

• Null Hypothesis (H 0 ): The average cholesterol level in a population is 200 mg/dL.
• Alternate Hypothesis (H 1 ): The average cholesterol level in a population is different from 200 mg/dL.

As the direction of deviation is not given , we assume a two-tailed test, and based on a normal distribution table, the critical values for a significance level of 0.05 (two-tailed) can be calculated through the z-table and are approximately -1.96 and 1.96.

Step 4: Result

Since the absolute value of the test statistic (2.04) is greater than the critical value (1.96), we reject the null hypothesis. And conclude that, there is statistically significant evidence that the average cholesterol level in the population is different from 200 mg/dL

Limitations of Hypothesis Testing

• Although a useful technique, hypothesis testing does not offer a comprehensive grasp of the topic being studied. Without fully reflecting the intricacy or whole context of the phenomena, it concentrates on certain hypotheses and statistical significance.
• The accuracy of hypothesis testing results is contingent on the quality of available data and the appropriateness of statistical methods used. Inaccurate data or poorly formulated hypotheses can lead to incorrect conclusions.
• Relying solely on hypothesis testing may cause analysts to overlook significant patterns or relationships in the data that are not captured by the specific hypotheses being tested. This limitation underscores the importance of complimenting hypothesis testing with other analytical approaches.

Hypothesis testing stands as a cornerstone in statistical analysis, enabling data scientists to navigate uncertainties and draw credible inferences from sample data. By systematically defining null and alternative hypotheses, choosing significance levels, and leveraging statistical tests, researchers can assess the validity of their assumptions. The article also elucidates the critical distinction between Type I and Type II errors, providing a comprehensive understanding of the nuanced decision-making process inherent in hypothesis testing. The real-life example of testing a new drug’s effect on blood pressure using a paired T-test showcases the practical application of these principles, underscoring the importance of statistical rigor in data-driven decision-making.

Frequently Asked Questions (FAQs)

1. what are the 3 types of hypothesis test.

There are three types of hypothesis tests: right-tailed, left-tailed, and two-tailed. Right-tailed tests assess if a parameter is greater, left-tailed if lesser. Two-tailed tests check for non-directional differences, greater or lesser.

2.What are the 4 components of hypothesis testing?

Null Hypothesis ( ): No effect or difference exists. Alternative Hypothesis ( ): An effect or difference exists. Significance Level ( ): Risk of rejecting null hypothesis when it’s true (Type I error). Test Statistic: Numerical value representing observed evidence against null hypothesis.

3.What is hypothesis testing in ML?

Statistical method to evaluate the performance and validity of machine learning models. Tests specific hypotheses about model behavior, like whether features influence predictions or if a model generalizes well to unseen data.

4.What is the difference between Pytest and hypothesis in Python?

Pytest purposes general testing framework for Python code while Hypothesis is a Property-based testing framework for Python, focusing on generating test cases based on specified properties of the code.

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Descriptive statistics, inferential statistics, stat reference, statistics - hypothesis testing a mean.

A population mean is an average of value a population.

Hypothesis tests are used to check a claim about the size of that population mean.

Hypothesis Testing a Mean

The following steps are used for a hypothesis test:

• Check the conditions
• Define the claims
• Decide the significance level
• Calculate the test statistic

For example:

• Population : Nobel Prize winners
• Category : Age when they received the prize.

And we want to check the claim:

"The average age of Nobel Prize winners when they received the prize is more than 55"

By taking a sample of 30 randomly selected Nobel Prize winners we could find that:

The mean age in the sample (\(\bar{x}\)) is 62.1

The standard deviation of age in the sample (\(s\)) is 13.46

From this sample data we check the claim with the steps below.

1. Checking the Conditions

The conditions for calculating a confidence interval for a proportion are:

• The sample is randomly selected
• The population data is normally distributed
• Sample size is large enough

A moderately large sample size, like 30, is typically large enough.

In the example, the sample size was 30 and it was randomly selected, so the conditions are fulfilled.

Note: Checking if the data is normally distributed can be done with specialized statistical tests.

2. Defining the Claims

We need to define a null hypothesis (\(H_{0}\)) and an alternative hypothesis (\(H_{1}\)) based on the claim we are checking.

The claim was:

In this case, the parameter is the mean age of Nobel Prize winners when they received the prize (\(\mu\)).

The null and alternative hypothesis are then:

Null hypothesis : The average age was 55.

Alternative hypothesis : The average age was more than 55.

Which can be expressed with symbols as:

\(H_{0}\): \(\mu = 55 \)

\(H_{1}\): \(\mu > 55 \)

This is a ' right tailed' test, because the alternative hypothesis claims that the proportion is more than in the null hypothesis.

If the data supports the alternative hypothesis, we reject the null hypothesis and accept the alternative hypothesis.

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3. Deciding the Significance Level

The significance level (\(\alpha\)) is the uncertainty we accept when rejecting the null hypothesis in a hypothesis test.

The significance level is a percentage probability of accidentally making the wrong conclusion.

Typical significance levels are:

• \(\alpha = 0.1\) (10%)
• \(\alpha = 0.05\) (5%)
• \(\alpha = 0.01\) (1%)

A lower significance level means that the evidence in the data needs to be stronger to reject the null hypothesis.

There is no "correct" significance level - it only states the uncertainty of the conclusion.

Note: A 5% significance level means that when we reject a null hypothesis:

We expect to reject a true null hypothesis 5 out of 100 times.

4. Calculating the Test Statistic

The test statistic is used to decide the outcome of the hypothesis test.

The test statistic is a standardized value calculated from the sample.

The formula for the test statistic (TS) of a population mean is:

\(\displaystyle \frac{\bar{x} - \mu}{s} \cdot \sqrt{n} \)

\(\bar{x}-\mu\) is the difference between the sample mean (\(\bar{x}\)) and the claimed population mean (\(\mu\)).

\(s\) is the sample standard deviation .

\(n\) is the sample size.

In our example:

The claimed (\(H_{0}\)) population mean (\(\mu\)) was \( 55 \)

The sample mean (\(\bar{x}\)) was \(62.1\)

The sample standard deviation (\(s\)) was \(13.46\)

The sample size (\(n\)) was \(30\)

So the test statistic (TS) is then:

\(\displaystyle \frac{62.1-55}{13.46} \cdot \sqrt{30} = \frac{7.1}{13.46} \cdot \sqrt{30} \approx 0.528 \cdot 5.477 = \underline{2.889}\)

You can also calculate the test statistic using programming language functions:

With Python use the scipy and math libraries to calculate the test statistic.

With R use built-in math and statistics functions to calculate the test statistic.

5. Concluding

There are two main approaches for making the conclusion of a hypothesis test:

• The critical value approach compares the test statistic with the critical value of the significance level.
• The P-value approach compares the P-value of the test statistic and with the significance level.

Note: The two approaches are only different in how they present the conclusion.

The Critical Value Approach

For the critical value approach we need to find the critical value (CV) of the significance level (\(\alpha\)).

For a population mean test, the critical value (CV) is a T-value from a student's t-distribution .

This critical T-value (CV) defines the rejection region for the test.

The rejection region is an area of probability in the tails of the standard normal distribution.

Because the claim is that the population mean is more than 55, the rejection region is in the right tail:

The student's t-distribution is adjusted for the uncertainty from smaller samples.

This adjustment is called degrees of freedom (df), which is the sample size \((n) - 1\)

In this case the degrees of freedom (df) is: \(30 - 1 = \underline{29} \)

Choosing a significance level (\(\alpha\)) of 0.01, or 1%, we can find the critical T-value from a T-table , or with a programming language function:

With Python use the Scipy Stats library t.ppf() function find the T-Value for an \(\alpha\) = 0.01 at 29 degrees of freedom (df).

With R use the built-in qt() function to find the t-value for an \(\alpha\) = 0.01 at 29 degrees of freedom (df).

Using either method we can find that the critical T-Value is \(\approx \underline{2.462}\)

For a right tailed test we need to check if the test statistic (TS) is bigger than the critical value (CV).

If the test statistic is bigger than the critical value, the test statistic is in the rejection region .

When the test statistic is in the rejection region, we reject the null hypothesis (\(H_{0}\)).

Here, the test statistic (TS) was \(\approx \underline{2.889}\) and the critical value was \(\approx \underline{2.462}\)

Here is an illustration of this test in a graph:

Since the test statistic was bigger than the critical value we reject the null hypothesis.

This means that the sample data supports the alternative hypothesis.

And we can summarize the conclusion stating:

The sample data supports the claim that "The average age of Nobel Prize winners when they received the prize is more than 55" at a 1% significance level .

The P-Value Approach

For the P-value approach we need to find the P-value of the test statistic (TS).

If the P-value is smaller than the significance level (\(\alpha\)), we reject the null hypothesis (\(H_{0}\)).

The test statistic was found to be \( \approx \underline{2.889} \)

For a population proportion test, the test statistic is a T-Value from a student's t-distribution .

Because this is a right tailed test, we need to find the P-value of a t-value bigger than 2.889.

The student's t-distribution is adjusted according to degrees of freedom (df), which is the sample size \((30) - 1 = \underline{29}\)

We can find the P-value using a T-table , or with a programming language function:

With Python use the Scipy Stats library t.cdf() function find the P-value of a T-value bigger than 2.889 at 29 degrees of freedom (df):

With R use the built-in pt() function find the P-value of a T-Value bigger than 2.889 at 29 degrees of freedom (df):

Using either method we can find that the P-value is \(\approx \underline{0.0036}\)

This tells us that the significance level (\(\alpha\)) would need to be bigger than 0.0036, or 0.36%, to reject the null hypothesis.

This P-value is smaller than any of the common significance levels (10%, 5%, 1%).

So the null hypothesis is rejected at all of these significance levels.

The sample data supports the claim that "The average age of Nobel Prize winners when they received the prize is more than 55" at a 10%, 5%, or 1% significance level .

Note: An outcome of an hypothesis test that rejects the null hypothesis with a p-value of 0.36% means:

For this p-value, we only expect to reject a true null hypothesis 36 out of 10000 times.

Calculating a P-Value for a Hypothesis Test with Programming

Many programming languages can calculate the P-value to decide outcome of a hypothesis test.

Using software and programming to calculate statistics is more common for bigger sets of data, as calculating manually becomes difficult.

The P-value calculated here will tell us the lowest possible significance level where the null-hypothesis can be rejected.

With Python use the scipy and math libraries to calculate the P-value for a right tailed hypothesis test for a mean.

Here, the sample size is 30, the sample mean is 62.1, the sample standard deviation is 13.46, and the test is for a mean bigger than 55.

With R use built-in math and statistics functions find the P-value for a right tailed hypothesis test for a mean.

Left-Tailed and Two-Tailed Tests

This was an example of a right tailed test, where the alternative hypothesis claimed that parameter is bigger than the null hypothesis claim.

You can check out an equivalent step-by-step guide for other types here:

• Left-Tailed Test
• Two-Tailed Test

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Teach yourself statistics

Hypothesis Test for a Mean

This lesson explains how to conduct a hypothesis test of a mean, when the following conditions are met:

• The sampling method is simple random sampling .
• The sampling distribution is normal or nearly normal.

Generally, the sampling distribution will be approximately normally distributed if any of the following conditions apply.

• The population distribution is normal.
• The population distribution is symmetric , unimodal , without outliers , and the sample size is 15 or less.
• The population distribution is moderately skewed , unimodal, without outliers, and the sample size is between 16 and 40.
• The sample size is greater than 40, without outliers.

This approach consists of four steps: (1) state the hypotheses, (2) formulate an analysis plan, (3) analyze sample data, and (4) interpret results.

State the Hypotheses

Every hypothesis test requires the analyst to state a null hypothesis and an alternative hypothesis . The hypotheses are stated in such a way that they are mutually exclusive. That is, if one is true, the other must be false; and vice versa.

The table below shows three sets of hypotheses. Each makes a statement about how the population mean μ is related to a specified value M . (In the table, the symbol ≠ means " not equal to ".)

The first set of hypotheses (Set 1) is an example of a two-tailed test , since an extreme value on either side of the sampling distribution would cause a researcher to reject the null hypothesis. The other two sets of hypotheses (Sets 2 and 3) are one-tailed tests , since an extreme value on only one side of the sampling distribution would cause a researcher to reject the null hypothesis.

Formulate an Analysis Plan

The analysis plan describes how to use sample data to accept or reject the null hypothesis. It should specify the following elements.

• Significance level. Often, researchers choose significance levels equal to 0.01, 0.05, or 0.10; but any value between 0 and 1 can be used.
• Test method. Use the one-sample t-test to determine whether the hypothesized mean differs significantly from the observed sample mean.

Analyze Sample Data

Using sample data, conduct a one-sample t-test. This involves finding the standard error, degrees of freedom, test statistic, and the P-value associated with the test statistic.

SE = s * sqrt{ ( 1/n ) * [ ( N - n ) / ( N - 1 ) ] }

SE = s / sqrt( n )

• Degrees of freedom. The degrees of freedom (DF) is equal to the sample size (n) minus one. Thus, DF = n - 1.

t = ( x - μ) / SE

• P-value. The P-value is the probability of observing a sample statistic as extreme as the test statistic. Since the test statistic is a t statistic, use the t Distribution Calculator to assess the probability associated with the t statistic, given the degrees of freedom computed above. (See sample problems at the end of this lesson for examples of how this is done.)

Sample Size Calculator

As you probably noticed, the process of hypothesis testing can be complex. When you need to test a hypothesis about a mean score, consider using the Sample Size Calculator. The calculator is fairly easy to use, and it is free. You can find the Sample Size Calculator in Stat Trek's main menu under the Stat Tools tab. Or you can tap the button below.

Interpret Results

If the sample findings are unlikely, given the null hypothesis, the researcher rejects the null hypothesis. Typically, this involves comparing the P-value to the significance level , and rejecting the null hypothesis when the P-value is less than the significance level.

Test Your Understanding

In this section, two sample problems illustrate how to conduct a hypothesis test of a mean score. The first problem involves a two-tailed test; the second problem, a one-tailed test.

Problem 1: Two-Tailed Test

An inventor has developed a new, energy-efficient lawn mower engine. He claims that the engine will run continuously for 5 hours (300 minutes) on a single gallon of regular gasoline. From his stock of 2000 engines, the inventor selects a simple random sample of 50 engines for testing. The engines run for an average of 295 minutes, with a standard deviation of 20 minutes. Test the null hypothesis that the mean run time is 300 minutes against the alternative hypothesis that the mean run time is not 300 minutes. Use a 0.05 level of significance. (Assume that run times for the population of engines are normally distributed.)

Solution: The solution to this problem takes four steps: (1) state the hypotheses, (2) formulate an analysis plan, (3) analyze sample data, and (4) interpret results. We work through those steps below:

Null hypothesis: μ = 300

Alternative hypothesis: μ ≠ 300

• Formulate an analysis plan . For this analysis, the significance level is 0.05. The test method is a one-sample t-test .

SE = s / sqrt(n) = 20 / sqrt(50) = 20/7.07 = 2.83

DF = n - 1 = 50 - 1 = 49

t = ( x - μ) / SE = (295 - 300)/2.83 = -1.77

where s is the standard deviation of the sample, x is the sample mean, μ is the hypothesized population mean, and n is the sample size.

Since we have a two-tailed test , the P-value is the probability that the t statistic having 49 degrees of freedom is less than -1.77 or greater than 1.77. We use the t Distribution Calculator to find P(t < -1.77) is about 0.04.

• If you enter 1.77 as the sample mean in the t Distribution Calculator, you will find the that the P(t < 1.77) is about 0.04. Therefore, P(t >  1.77) is 1 minus 0.96 or 0.04. Thus, the P-value = 0.04 + 0.04 = 0.08.
• Interpret results . Since the P-value (0.08) is greater than the significance level (0.05), we cannot reject the null hypothesis.

Note: If you use this approach on an exam, you may also want to mention why this approach is appropriate. Specifically, the approach is appropriate because the sampling method was simple random sampling, the population was normally distributed, and the sample size was small relative to the population size (less than 5%).

Problem 2: One-Tailed Test

Bon Air Elementary School has 1000 students. The principal of the school thinks that the average IQ of students at Bon Air is at least 110. To prove her point, she administers an IQ test to 20 randomly selected students. Among the sampled students, the average IQ is 108 with a standard deviation of 10. Based on these results, should the principal accept or reject her original hypothesis? Assume a significance level of 0.01. (Assume that test scores in the population of engines are normally distributed.)

Null hypothesis: μ >= 110

Alternative hypothesis: μ < 110

• Formulate an analysis plan . For this analysis, the significance level is 0.01. The test method is a one-sample t-test .

SE = s / sqrt(n) = 10 / sqrt(20) = 10/4.472 = 2.236

DF = n - 1 = 20 - 1 = 19

t = ( x - μ) / SE = (108 - 110)/2.236 = -0.894

Here is the logic of the analysis: Given the alternative hypothesis (μ < 110), we want to know whether the observed sample mean is small enough to cause us to reject the null hypothesis.

The observed sample mean produced a t statistic test statistic of -0.894. We use the t Distribution Calculator to find P(t < -0.894) is about 0.19.

• This means we would expect to find a sample mean of 108 or smaller in 19 percent of our samples, if the true population IQ were 110. Thus the P-value in this analysis is 0.19.
• Interpret results . Since the P-value (0.19) is greater than the significance level (0.01), we cannot reject the null hypothesis.

Science and the scientific method: Definitions and examples

Here's a look at the foundation of doing science — the scientific method.

The scientific method

Hypothesis, theory and law, a brief history of science, additional resources, bibliography.

Science is a systematic and logical approach to discovering how things in the universe work. It is also the body of knowledge accumulated through the discoveries about all the things in the universe. 

The word "science" is derived from the Latin word "scientia," which means knowledge based on demonstrable and reproducible data, according to the Merriam-Webster dictionary . True to this definition, science aims for measurable results through testing and analysis, a process known as the scientific method. Science is based on fact, not opinion or preferences. The process of science is designed to challenge ideas through research. One important aspect of the scientific process is that it focuses only on the natural world, according to the University of California, Berkeley . Anything that is considered supernatural, or beyond physical reality, does not fit into the definition of science.

When conducting research, scientists use the scientific method to collect measurable, empirical evidence in an experiment related to a hypothesis (often in the form of an if/then statement) that is designed to support or contradict a scientific theory .

"As a field biologist, my favorite part of the scientific method is being in the field collecting the data," Jaime Tanner, a professor of biology at Marlboro College, told Live Science. "But what really makes that fun is knowing that you are trying to answer an interesting question. So the first step in identifying questions and generating possible answers (hypotheses) is also very important and is a creative process. Then once you collect the data you analyze it to see if your hypothesis is supported or not."

The steps of the scientific method go something like this, according to Highline College :

• Make an observation or observations.
• Form a hypothesis — a tentative description of what's been observed, and make predictions based on that hypothesis.
• Test the hypothesis and predictions in an experiment that can be reproduced.
• Analyze the data and draw conclusions; accept or reject the hypothesis or modify the hypothesis if necessary.
• Reproduce the experiment until there are no discrepancies between observations and theory. "Replication of methods and results is my favorite step in the scientific method," Moshe Pritsker, a former post-doctoral researcher at Harvard Medical School and CEO of JoVE, told Live Science. "The reproducibility of published experiments is the foundation of science. No reproducibility — no science."

Some key underpinnings to the scientific method:

• The hypothesis must be testable and falsifiable, according to North Carolina State University . Falsifiable means that there must be a possible negative answer to the hypothesis.
• Research must involve deductive reasoning and inductive reasoning . Deductive reasoning is the process of using true premises to reach a logical true conclusion while inductive reasoning uses observations to infer an explanation for those observations.
• An experiment should include a dependent variable (which does not change) and an independent variable (which does change), according to the University of California, Santa Barbara .
• An experiment should include an experimental group and a control group. The control group is what the experimental group is compared against, according to Britannica .

The process of generating and testing a hypothesis forms the backbone of the scientific method. When an idea has been confirmed over many experiments, it can be called a scientific theory. While a theory provides an explanation for a phenomenon, a scientific law provides a description of a phenomenon, according to The University of Waikato . One example would be the law of conservation of energy, which is the first law of thermodynamics that says that energy can neither be created nor destroyed. 

A law describes an observed phenomenon, but it doesn't explain why the phenomenon exists or what causes it. "In science, laws are a starting place," said Peter Coppinger, an associate professor of biology and biomedical engineering at the Rose-Hulman Institute of Technology. "From there, scientists can then ask the questions, 'Why and how?'"

Laws are generally considered to be without exception, though some laws have been modified over time after further testing found discrepancies. For instance, Newton's laws of motion describe everything we've observed in the macroscopic world, but they break down at the subatomic level.

This does not mean theories are not meaningful. For a hypothesis to become a theory, scientists must conduct rigorous testing, typically across multiple disciplines by separate groups of scientists. Saying something is "just a theory" confuses the scientific definition of "theory" with the layperson's definition. To most people a theory is a hunch. In science, a theory is the framework for observations and facts, Tanner told Live Science.

The earliest evidence of science can be found as far back as records exist. Early tablets contain numerals and information about the solar system , which were derived by using careful observation, prediction and testing of those predictions. Science became decidedly more "scientific" over time, however.

1200s: Robert Grosseteste developed the framework for the proper methods of modern scientific experimentation, according to the Stanford Encyclopedia of Philosophy. His works included the principle that an inquiry must be based on measurable evidence that is confirmed through testing.

1400s: Leonardo da Vinci began his notebooks in pursuit of evidence that the human body is microcosmic. The artist, scientist and mathematician also gathered information about optics and hydrodynamics.

1500s: Nicolaus Copernicus advanced the understanding of the solar system with his discovery of heliocentrism. This is a model in which Earth and the other planets revolve around the sun, which is the center of the solar system.

1600s: Johannes Kepler built upon those observations with his laws of planetary motion. Galileo Galilei improved on a new invention, the telescope, and used it to study the sun and planets. The 1600s also saw advancements in the study of physics as Isaac Newton developed his laws of motion.

1700s: Benjamin Franklin discovered that lightning is electrical. He also contributed to the study of oceanography and meteorology. The understanding of chemistry also evolved during this century as Antoine Lavoisier, dubbed the father of modern chemistry , developed the law of conservation of mass.

1800s: Milestones included Alessandro Volta's discoveries regarding electrochemical series, which led to the invention of the battery. John Dalton also introduced atomic theory, which stated that all matter is composed of atoms that combine to form molecules. The basis of modern study of genetics advanced as Gregor Mendel unveiled his laws of inheritance. Later in the century, Wilhelm Conrad Röntgen discovered X-rays , while George Ohm's law provided the basis for understanding how to harness electrical charges.

1900s: The discoveries of Albert Einstein , who is best known for his theory of relativity, dominated the beginning of the 20th century. Einstein's theory of relativity is actually two separate theories. His special theory of relativity, which he outlined in a 1905 paper, " The Electrodynamics of Moving Bodies ," concluded that time must change according to the speed of a moving object relative to the frame of reference of an observer. His second theory of general relativity, which he published as " The Foundation of the General Theory of Relativity ," advanced the idea that matter causes space to curve.

In 1952, Jonas Salk developed the polio vaccine , which reduced the incidence of polio in the United States by nearly 90%, according to Britannica . The following year, James D. Watson and Francis Crick discovered the structure of DNA , which is a double helix formed by base pairs attached to a sugar-phosphate backbone, according to the National Human Genome Research Institute .

2000s: The 21st century saw the first draft of the human genome completed, leading to a greater understanding of DNA. This advanced the study of genetics, its role in human biology and its use as a predictor of diseases and other disorders, according to the National Human Genome Research Institute .

• This video from City University of New York delves into the basics of what defines science.
• Learn about what makes science science in this book excerpt from Washington State University .
• This resource from the University of Michigan — Flint explains how to design your own scientific study.

Merriam-Webster Dictionary, Scientia. 2022. https://www.merriam-webster.com/dictionary/scientia

University of California, Berkeley, "Understanding Science: An Overview." 2022. ​​ https://undsci.berkeley.edu/article/0_0_0/intro_01  

Highline College, "Scientific method." July 12, 2015. https://people.highline.edu/iglozman/classes/astronotes/scimeth.htm  

North Carolina State University, "Science Scripts." https://projects.ncsu.edu/project/bio183de/Black/science/science_scripts.html  

University of California, Santa Barbara. "What is an Independent variable?" October 31,2017. http://scienceline.ucsb.edu/getkey.php?key=6045  

Encyclopedia Britannica, "Control group." May 14, 2020. https://www.britannica.com/science/control-group  

The University of Waikato, "Scientific Hypothesis, Theories and Laws." https://sci.waikato.ac.nz/evolution/Theories.shtml  

Stanford Encyclopedia of Philosophy, Robert Grosseteste. May 3, 2019. https://plato.stanford.edu/entries/grosseteste/  

Encyclopedia Britannica, "Jonas Salk." October 21, 2021. https://www.britannica.com/ biography /Jonas-Salk

National Human Genome Research Institute, "​Phosphate Backbone." https://www.genome.gov/genetics-glossary/Phosphate-Backbone  

National Human Genome Research Institute, "What is the Human Genome Project?" https://www.genome.gov/human-genome-project/What  

‌ Live Science contributor Ashley Hamer updated this article on Jan. 16, 2022.

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Statistics By Jim

Making statistics intuitive

Ordinal Data: Definition, Examples & Analysis

By Jim Frost 5 Comments

What is Ordinal Data?

Ordinal data have at least three categories that have a natural rank order. The categories are ranked, but the differences between ranks may not be equal. These data indicate the order of values but not the degree of difference between them. For example, first, second, and third places in a race are ordinal data. You can clearly understand the order of finishes. However, the time difference between first and second place might not be the same as between second and third place.

Ordinal data are prevalent in social science and survey research. These variables are relatively convenient for respondents to choose even when the underlying variable is complex, allowing you to compare the participants. For example, subject-area expertise can be tricky to measure using a continuous scale. However, ordinal data can make this evaluation much easier by using Beginner, Intermediate, and Expert ranking choices in a survey.

Likert scale items in a survey are ordinal data. These items typically have 5 or 7 possible responses.

While this data type is expedient, it has downsides that limit the valid summary values and analyses you can use. More on this later!

Learn more about Likert Scale: Survey Use & Examples .

Ordinal Data Examples

The key concept behind ordinal data is that it ranks observations. However, these ranks don’t indicate the relative degree of difference between two observations. For instance, you know that a high-income person earns more than a middle-income individual, but you don’t know how much more they make. Keep that in mind as you consider the following examples.

Comparisons to Other Types of Variables

Ordinal data share properties with both nominal and continuous variables yet are distinct from either.

Nominal vs Ordinal Data

Ordinal and nominal data are discrete variables that define categories. Consequently, statisticians consider both types to be qualitative data.

However, you can rank ordinal data, which is impossible with nominal data.

For example, college major is nominal data; you can’t rank those categories using that variable alone. They’re simply names of distinct groups, such as statistics, political science, and psychology.

Conversely, ordinal data form groups that you can inherently rank. For example, the relative size of college majors at an institution can be small, medium, or large.

Related post : Discrete vs Continuous Variables

Continuous vs Ordinal

Ordinal and continuous data (both interval and ratio scale) can rank observations on a scale. In other words, you can record that one observation has more of a characteristic than another observation. However, as discussed earlier, ordinal data can’t describe the degree of difference between values, while a continuous variable can.

For example, the size of a college major at an institution can be small, medium, or large. If one major is large and another medium, you see that the former is larger than the latter. However, you don’t know the degree of difference.

Conversely, if you measure size using a continuous variable such as the number of students or budget, you can determine the degree of difference between two observations.

In some cases, you can choose to measure a variable either as continuous or ordinal data. Whenever practical, choose the continuous form because it retains more information and gives you more options during the analysis.

Amongst the various measurement scales, ordinal data fall between the nominal and interval scales. For more information, read Nominal, Ordinal, Interval, and Ratio Scales .

Ordinal Data Limitations

The inability to know the precise differences between observations limits the mathematical functions and summary statistics you can calculate for ordinal data.

While analysts often record values for these variables using numbers, such as 1-5 for a Likert scale of agreement, that doesn’t indicate all numeric calculations are valid.

You cannot meaningfully add and subtract values. For example, if you take ordinal data values of 1 and 2, you can’t trust that summing them to 3 is a valid result. Why?

When adding 1 and 2 to get 3, you’re assuming the difference between 1 and 2 equals the difference between 2 and 3 because they’re both one unit apart. However, that is not a safe assumption with this data type.

Because addition isn’t valid, you can’t subtract because it’s the inverse function. Also, calculating the mean is invalid because it involves addition and division (also invalid). Division is valid only for continuous variables using a ratio scale.

Analyzing Ordinal Data

So, what can you do with these variables? Which summary statistics are valid? And what kind of analyses can you perform?

Bar graphs are great for displaying discrete variables. Consequently, they’re an excellent choice for visually understanding ordinal data.

The bar chart below displays a Likert scale item for service ratings from Very Poor to Very Good.

It’s easy to see that most patrons rated the service as Good.

Learn more about Bar Charts and Data Types and How to Graph Them .

Summary Statistics

Measures of central tendency and variability are two standard summary statistics to report with your results. However, the mean and standard deviation are questionable for ordinal data. Consequently, consider using the following alternatives:

• Mode and Median for the central tendency.
• Range , interquartile range , and interval for two percentiles  for variability.

Click the links to learn more about these concepts and statistics.

Hypothesis Testing

Similarly, the standard hypothesis tests for the mean (e.g., t-tests and ANOVA) are questionable for this type of variable. Means tests are parametric hypothesis tests.

Instead, consider using nonparametric hypothesis tests as an alternative. They assess medians and ranks, making them perfect for ordinal data. These tests include Mood’s Median, Mann-Whitney, Wilcoxon, Friedman’s Test, and Spearman’s rho.

For more information, read my post about Parametric vs Nonparametric Hypothesis Tests and Spearman’s rho .

Finally, statisticians have some disputes over using hypothesis tests for the mean with Likert scale items. I discussed the critical reason in this post—the mean is not valid. However, some make the case that for specific Likert scales the differences between values are equal by design. If that is true, then the mean might be valid.

However, for parametric hypothesis testing, there are additional concerns for ordinal data. Specifically, these variables are less likely to satisfy the analysis’ assumptions. To learn more about this issue, including some answers about what to do, read my post about Analyzing Likert Scale Data .

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Reader Interactions

November 29, 2023 at 10:37 am

Hi Jim, I have a question regarding a variable I am working on. What type of variable would you consider “grade average in sience” to be? I can see it being both ordinal and continious (interval). In my study I am going to investigate whether students confidence in science, and the amount of time they spend reading can predict their grade average at the end of the school year.

October 18, 2022 at 3:23 pm

Hi, thank you for the great post! I also have a question. You say that mean and standard deviation are questionable for ordinal data. Although my groups are statistically different, the median for all groups is equal (=2), meaning that all my graph bars, which show the median, look the same. What should I do instead? I would have preferred to use the mean and SD since it’s easier for the reader to see a difference among the groups.

October 19, 2022 at 1:30 am

When you say that your groups are statistically significant, what do you mean? How are they different? That’ll help me answer your question.

If the frequency distributions for both groups look similar, they might not be significantly different. You can perform a nonparametric test to see test whether the means are different . If the distribution looks the same as you say, the means might not be different either.

If you can safely assume that the differences between each value in your ordinal scale are spaced equidistantly, some analysts think it’s ok to assess the mean. There is some debate among analysts over that, but some do that. So, if you can make that assumption, you might be ok with the mean and standard deviation.

September 6, 2022 at 2:22 am

Great post! Question. I have several ordinal variables which theoretically are all associated with well-being. They are measured on different scales – some 5 point and some 7 points. I want to create a composite variable. Your post made me wonder about the use of z scores for ordinal variables? I had thought about creating z scores for each variable and summing to create one score.

October 3, 2022 at 4:15 pm

Sorry about the delay in replying. Sometimes things slip through the cracks!

Z-scores are specifically for continuous data that follow a normal distribution. So, it’s not appropriate for ordinal data. I suppose if your ordinal data followed a normal distribution, you might be able to use it for that purpose, but they are frequently non-normal.

Frequently, analysts will add a set of ordinal/Likert scores together, or average them, to create a composite variable. I’d probably go with that approach.

Although there is debate about whether an average is appropriate with ordinal data unless you’re sure that the values are equidistant. If the values are not equidistant, the average can be meaningless. The median is universally accepted as appropriate for ordinal data.

The technical details of ordinal/Likert can be a bit complicated and there is some debate about which measures and analyses are acceptable. But often analysts use a composite sum, average, or median of a set of Likert/ordinal values.

Please read my post about analyzing Likert scale data for a comparison between parametric vs. nonparametric analyses. Although, that post applies to individual items rather than a sum.

One of the reasons I point out these complications is because what is considered acceptable for ordinal data can vary by subject area and analysts. So, I’d also look into finding the acceptable norms for your situation.

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What is Amazon?

Amazon (Amazon.com) is the world's largest online retailer and a prominent cloud service provider .

Originally started as an online bookselling company, Amazon has morphed into an internet-based business enterprise that is largely focused on providing e-commerce, cloud computing, digital streaming and artificial intelligence ( AI ) services.

Following an Amazon-to-buyer sales approach, the company offers a monumental product range and inventory, enabling consumers to buy just about anything, including clothing, beauty supplies, gourmet food, jewelry, books, movies, electronics, pet supplies, furniture, toys, garden supplies and household goods.

Headquartered in Seattle, Amazon has individual websites, software development centers, customer service centers, data centers and fulfillment centers around the world.

History and timeline of Amazon

Amazon has come a long way since it was founded by Jeff Bezos in his garage in Bellevue, Wash., on July 5, 1994.

The following is a brief history and timeline of events that have evolved Amazon from its humble beginnings to a multinational business empire.

Amazon officially opened for business as an online bookseller on July 16, 1995. Originally, Bezos had incorporated the company as Cadabra but later changed the name to Amazon. Bezos is said to have browsed a dictionary for a word beginning with A for the value of alphabetic placement. He selected the name Amazon because it was exotic and different and as a reference to his plan for the company's size to reflect that of the Amazon River, one of the largest rivers in the world. Since its inception, the company's motto has always been "get big fast."

In 2005, Amazon Prime This membership-based service for Amazon customers offers free two-day shipping within the contiguous U.S., as well as streaming, shopping and reading benefits. According to Amazon's website, current Amazon Prime membership rates are $14.99 a month or $139 per year.

Amazon Web Services

This comprehensive and evolving cloud computing platform was also born in the 2000s. The first Amazon Web Services ( AWS ) offerings were launched in 2006 to provide online services for websites and client-side applications. Amazon Elastic Compute Cloud ( EC2 ) and Simple Storage Service ( S3 ) are the backbones of the company's growing collection of web services. The same year, Amazon also launched a cloud computing and video-on-demand service known at the time as Unbox. By changing the way people bought books, Amazon also shaped how they read them with the launch of its first Kindle e-reader in 2007. This device helps users browse, buy and read e-books, magazines and newspapers from the Kindle Store.

From the 2010s to present

Amazon debuted its first tablet computer, the Kindle Fire, in 2011 and the Amazon Fire TV Stick, which is part of Amazon's extensive line of streaming media devices, in 2014. Amazon also started an online Amazon Art marketplace for fine arts in 2013, which has featured original works by famous artists such as Claude Monet and Norman Rockwell. The popular in-home virtual assistant Amazon Alexa was rolled out to consumers in 2015 and was followed by the Alexa-equipped Echo Dot in 2016. Amazon acquired the organic grocery store Whole Foods in 2017 and launched Amazon Go, a chain of cashierless grocery stores in 2018. The rise of in-home shopping during the COVID-19 pandemic made consumers rely on Amazon even more, and the trend is likely to keep growing.

Notable Amazon products and services

Amazon offers an ever-expanding portfolio of services and products. Following is a list of its noteworthy offerings.

• Amazon Marketplace. Amazon's e-commerce platform enables third-party retailers to showcase and sell their products alongside Amazon items.
• Amazon Fresh. Amazon's grocery pickup and delivery service is currently available in nearly two dozen U.S. cities and a few international locations. A grocery order can be placed through the Amazon Fresh website or the Amazon mobile app. Customers can either get their groceries delivered or visit the store for pickup.
• Amazon Vine. Launched in 2007, Amazon Vine helps manufacturers and publishers get reviews for their products to help shoppers make informed purchases.
• Woot. Acquired by Amazon in 2010, Woot offers limited time offers and special deals that rotate daily. This shop features refurbished items, as well as new items that are low in stock. Prime members get free shipping.
• Zappos. Amazon bought Zappos in 2009. This online retailer of shoes and clothing carries a wide range of brands, including Nike, Sperry, Adidas and Uggs.
• Merch by Amazon. This on-demand T-shirt printing service enables sellers to create and upload their T-shirt designs for free and earn royalties on each sale. Amazon does the rest -- from printing the T-shirts to delivering them to customers.
• Amazon Handmade. This platform enables artisans to sell handcrafted products to customers around the world.

Consumer technology

• Amazon Kindle. Amazon's first e-reader, Kindle, enables users to browse, buy and read e-books, magazines and newspapers from the Kindle Store.
• Amazon Fire tablet. Previously known as Kindle Fire, Amazon's popular and high-profile Fire tablet competes with Apple's iPad.
• Amazon Fire TV. This line of Amazon's streaming media players and digital devices delivers streamed video content over the internet to a paired high-definition television.
• Amazon Alexa. This cloud-based, AI-powered, voice-controlled personal assistant is designed to answer queries, interact with users, and perform other tasks and commands.
• Amazon Echo. This is one of Amazon's smart home devices that comes equipped with a speaker and connects to Alexa. Amazon Echo can perform several functions, including talking about the weather, creating shopping lists and controlling other smart products, such as lights, switches and televisions.
• Amazon Echo Dot. A smaller, puck-shaped version of the original Amazon Echo, an Echo Dot can be placed in any room and can answer questions, play music, and read news and other stories.
• Amazon Echo Show. As part of the Amazon Echo line of speakers, the Amazon Echo Show works similarly through Alexa but also offers a 7-inch touchscreen display to play videos and music and conduct video calls with other Echo users.
• Amazon Astro. This is Amazon's first home monitoring robot that works with Alexa. It is designed to help with various household tasks, such as home monitoring, caring for the elderly through notifications and alerts, and following owners from room to room to play TV shows, music or podcasts.

Subscription services

• Amazon Prime. This subscription service provides members access to exclusive shopping and entertainment services, discounts and more. As an example, all Amazon Prime members enjoy free one-day or two-day shipping on qualifying orders.
• Amazon Prime Video. This is Amazon's on-demand video streaming service that offers a selection of about 24,000 movies and over 2,100 TV shows. This service is included with an Amazon Prime membership.
• Amazon Drive. Previously known as Amazon Cloud Drive, Amazon Drive is a cloud storage app that offers 5 gigabytes (GB) of free and secure online storage for photos, videos and files for Amazon customers. Amazon Prime members get free, unlimited, full-resolution photo storage, along with 5 GB of video storage.
• Twitch Prime. A monthly subscription service, Twitch Prime is a subsidiary of Amazon Prime. It gives members premium access to Twitch -- a video streaming platform that offers a fun and social way to watch people play games.
• Amazon Music Prime. This is Amazon's music streaming service that is free for Prime members.

Digital content

• Amazon Pay. An online transaction processing platform, Amazon Pay enables Amazon account holders to use their Amazon accounts to pay external online merchants.
• Amazon Music Unlimited. Amazon's premium music service costs $8.99 a month for Prime members and $9.99 for non-Prime members.
• Kindle Store. Part of Amazon's retail website, the Kindle Store can be accessed from any Kindle device to purchase e-books.
• Amazon Appstore for Android. Amazon's app store for the Android operating system enables users to download games and mobile apps to supported devices.
• S3. This is Amazon's scalable, cloud-based object storage. Files are referred to as objects in S3 and are stored in containers called buckets .
• Amazon Simple Queue Service (SQS). SQS is a pay-per-use web service that is designed to provide access to a waiting message queue where messages can reside until a computer processes them.
• Amazon EC2. This web service interface provides scalability with resizable compute capacity in the AWS cloud. Users can run virtual servers or instances, commonly known as EC2 instances, that can be scaled up or down, depending on the network requirements.
• Amazon S3 Glacier. Amazon S3 Glacier is a low-cost cloud storage service for data that might be associated with longer retrieval times. It also offers data archiving and backup of cold data.
• AWS Identity and Access Management (IAM). IAM provides secure and controlled access to resources.
• Amazon Redshift. This data warehouse in the cloud enables users to query petabytes of both structured and semistructured data using standard Structured Query Language queries. For example, to address the expanding volume of transactions, Nasdaq moved from a legacy on-premises data center to the AWS cloud, which is powered through the Amazon Redshift cluster.

Amazon AI services

• Amazon SageMaker. A fully managed cloud machine learning platform, Amazon SageMaker enables developers and data scientists to build, train and deploy machine learning models for predictive analytics applications.
• Amazon Lex. This service for building conversational interfaces into any application using voice and text is powered by the same technology as Alexa.
• Amazon Polly. A text-to-speech service, Amazon Polly uses deep learning technology to convert text into spoken audio. It includes 60 voices across 29 languages.
• Amazon Rekognition. This software-as-a-service facial recognition and analysis platform uses a deep learning algorithm to process images and extract information from them.
• AWS DeepLens. This programmable video camera enables developers to easily experiment withmachine learning, AI and the internet of things .
• Alexa Voice Service. This programming interface provides developers with a set of C++ libraries to add Amazon Alexa's speech and other capabilities into their applications and devices.
• Amazon Transcribe. This service converts speech to text quickly and accurately by using a deep learning process called automatic speech recognition .
• Amazon Translate. Amazon Translate is a cloud service that can convert large amounts of text written in one language to another language.
• Alexa Skills Kit. This software development kit enables developers to build skills or conversational applications on Amazon Alexa.

Amazon privately owned brands

• AmazonBasics. This is Amazon's privately labeled, low-budget brand that mainly sells kitchen, tech and household products.
• Amazon Elements. This line of domestic products includes health and personal care items, as well as nutritional supplements.
• Mama Bear. This private label of Amazon sells baby wipes, newborn through size 6 diapers, baby food, diaper pail refills and baby laundry detergent.
• Presto!. This brand started as a laundry detergent in 2016 but has added household paper towels and toilet paper to its product line.
• Amazon Essentials. A Prime-exclusive program, this clothing line offers basic wear for men, women, babies and kids, with additional options for family, big and tall, and athletic activity.
• Happy Belly. This private label of Amazon was introduced in 2016 and sells snack food items. In February 2019, the brand also began offering milk delivery services.
• Goodthreads. This menswear apparel line is available exclusively to Amazon Prime members. The label offers both casual and professional pieces and is deemed a bit higher quality and more stylish than the Amazon Essentials brand.

Notable Amazon subsidiaries and acquisitions

From healthcare to entertainment, Amazon has acquired multiple companies by tapping into a variety of sectors over time.

Following is a list of Amazon's notable acquisitions and subsidiary companies:

• IMDb. The world's most popular database for movies, TV, celebrity, video games and streaming online content was acquired by Amazon in 1998.
• Audible. Audible, a book and spoken audio content provider, was acquired by Amazon in 2008 for $300 million.
• Zappos. Amazon acquired this online shoe and clothing retailer in an all-stock deal worth $1.2 billion in 2009.
• Twitch. A social media and video game streaming platform, Twitch was purchased by Amazon for $970 million in 2014.
• Whole Foods. Food, beverage and organic grocery store chain Whole Foods was acquired by Amazon for $13.7 billion in 2017.
• Ring. Amazon took ownership of this home security and smart home company in 2018 for $1 billion.
• Zoox. An autonomous vehicles , robotics and transportation company was acquired as a wholly owned subsidiary by Amazon for $1.2 billion in 2020.
• Metro-Goldwyn-Mayer. Amazon acquired this film and TV studio for $8.5 billion in March 2022.

Amazon controversies and criticisms

Amazon has suffered a massive backlash over the years from multiple sources. The tech giant is also being held responsible for creating the Amazon effect -- the evolution and disruption of the retail market due to the company exhibiting monopolistic behaviors.

Following are a few concerns and allegations that Amazon has faced over time:

• Monopolistic and anticompetitive behavior. Due to Amazon's size and economies of scale, it has been outpricing local and small shopkeepers and is accused of displacing an open market with a privately controlled one. This is leading to the slow death of the brick-and-mortar store model built by companies such as Sears and J.C. Penney.
• Unfair treatment of workers. Amazon is frequently under the microscope for providing unfair work conditions in its warehouses, including treating workers as robots, providing low wages and creating unsafe work conditions.
• Huge carbon footprint. Over the past two decades, Amazon has been accused by environmental activists of having a staggering carbon footprint . Transport of any merchandise relies on oil, and since Amazon delivers anything everywhere, it leaves a long-lasting carbon footprint that automatically falls on its shoulders.
• E-waste. A recent investigation conducted by British television network ITV uncovered how Amazon is contributing to the world's e-waste crisis by destroying millions of unused or returned products. This also includes millions of electronics, such as phones, computers and TVs that are toxic to soil, water, air and wildlife.
• Counterfeit product listings. Amazon has been under scrutiny by brands, shoppers and lawmakers as counterfeiters have been listing and selling fake products on Amazon through its third-party marketplace. To crack down on counterfeit products on its site, Amazon destroyed 2 million counterfeit products sent to its warehouses and blocked 10 billion fake listings in 2021.
• Avoiding taxation. Edging fast toward a monopoly status, Amazon has been criticized for often avoiding tax payments despite making huge profits. According to a report by the Institute on Taxation and Economic Policy, the company avoided around $5.2 billion in corporate federal income taxes in 2021.

Amazon finances

According to a news release posted on Amazon's investor relations website , Amazon experienced a significant increase in net sales but a decrease in operating income in the first quarter of 2022.

Following are some notable statistics from the release:

• Net sales increased 7% to $116.4 billion in the first quarter, compared with $108.5 billion in the first quarter of 2021. Excluding the $1.8 billion unfavorable impacts from year-over-year changes in foreign exchange rates throughout the quarter, net sales increased 9% compared with the first quarter of 2021.
• Operating income decreased to $3.7 billion in the first quarter, compared with $8.9 billion in the first quarter of 2021.
• Net loss was $3.8 billion in the first quarter compared with net income of $8.1 billion in the first quarter of 2021.

Besides being recognized as a company with business interests in e-commerce, cloud computing and AI services, Amazon also offers an extensive list of subscription services . Learn about these services and the perks they offer.

Continue Reading About Amazon

• Break down the components of Amazon's retail business
• MSP business trends, from e-waste to virtual warehouse
• Amazon's impact on publishing transforms the book industry
• New Amazon grocery stores run on computer vision, apps
• Twitch confirms data breach following massive leak

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• Patient Care & Health Information
• Tests & Procedures
• C-reactive protein test

C-reactive protein (CRP) is a protein made by the liver. The level of CRP increases when there's inflammation in the body. A simple blood test can check your C-reactive protein level.

A high-sensitivity C-reactive protein (hs-CRP) test is more sensitive than a standard C-reactive protein test. That means the high-sensitivity test can find smaller increases in C-reactive protein than a standard test can.

The hs-CRP test can help show the risk of getting coronary artery disease. In coronary artery disease, the arteries of the heart narrow. Narrowed arteries can lead to a heart attack.

Why it's done

Your health care provider might order a C-reactive protein test to:

• Check for infection.
• Help diagnose a chronic inflammatory disease, such as rheumatoid arthritis or lupus.
• Learn your risk of heart disease.
• Learn your the risk of a second heart attack.

A note about CRP tests for heart disease

A high level of hs-CRP in the blood has been linked to an increased risk of heart attacks. Also, people who have had a heart attack are more likely to have another heart attack if they have a high hs-CRP level. But their risk goes down when their hs-CRP level is in the typical range.

An hs-CRP test isn't for everyone. The test doesn't show the cause of inflammation. So it's possible to have a high hs-CRP level without it affecting the heart.

An hs-CRP test may be most useful for people who have a 10% to 20% chance of having a heart attack within the next 10 years. This is known as intermediate risk. A health care provider can determine your risk using tests that look at your lifestyle choices, family history and overall health.

More Information

• Adult Still disease
• Giant cell arteritis
• Juvenile idiopathic arthritis
• Polymyalgia rheumatica
• Takayasu's arteritis

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How you prepare

Hard exercise, such as intense weight training or a long run, can cause a sudden jump in the C-reactive protein level. Your health care provider might ask you to avoid such activities before the test.

Some medicines can affect CRP level. Tell your care provider about the medicines you take, including those you bought without a prescription.

If your blood sample will be used for other tests, you may need to avoid food or drink for a period before the test. For example, if you're having an hs-CRP test to check for heart disease, you might have a cholesterol test, which requires fasting, at the same time.

Your health care provider tells you how to prepare for your test.

What you can expect

To take a sample of your blood, a health care provider places a needle into a vein in your arm, usually at the bend of the elbow. The blood sample goes to a lab for analysis. You can return to your usual activities right away.

It can take a few days to get results. Your health care provider can explain what the test results mean.

C-reactive protein is measured in milligrams per liter (mg/L). Results equal to or greater than 8 mg/L or 10 mg/L are considered high. Range values vary depending on the lab doing the test.

A high test result is a sign of inflammation. It may be due to serious infection, injury or chronic disease. Your health care provider may recommend other tests to determine the cause.

Results for an hs-CRP test are usually given as follows:

• Lower risk of heart disease: Less than 2.0 mg/L
• Higher risk of heart disease: Equal to or greater than 2.0 mg/L

A person's CRP levels vary over time. A coronary artery disease risk assessment should be based on the average of two hs-CRP tests. It's best if they're taken two weeks apart. Values above 2.0 mg/L may mean an increased risk of heart attacks or risk of a repeat heart attack.

Hs-CRP level is only one risk factor for coronary artery disease. Having a high hs-CRP level doesn't always mean a higher risk of developing heart disease. Other tests results can help determine the risk.

Talk to your health care provider about your risk factors for heart disease and ways to try to prevent it. Lifestyle changes or medicines might help lower the risk of a heart attack.

• Arnett DK, et al. 2019 ACC/AHA guideline on the primary prevention of cardiovascular disease: A report of the American College of Cardiology/American Heart Association Task Force on Clinical Practice Guidelines. Circulation. 2019; doi:10.1161/CIR.0000000000000678.
• Filippo C, et al. C-reactive protein in cardiovascular disease. https://www.uptodate.com/contents/search. Accessed Nov. 18, 2022.
• Wilson PWF, et al. Overview of established risk factors for cardiovascular disease. https://www.uptodate.com/contents/search. Accessed April 13, 2021.
• C-reactive protein, high sensitivity, serum. Mayo Clinic Laboratories. https://www.mayocliniclabs.com/test-catalog/Clinical+and+Interpretive/82047. Accessed Nov. 15, 2022.
• Ferri FF. C-reactive protein. In: Ferri's Best Test: A Practical Guide to Clinical Laboratory Medicine and Diagnostic Imaging. 5th ed. Elsevier; 2023. https://www.clinicalkey.com. Accessed Nov. 15, 2022.
• Lopez-Jimenez F (expert opinion). Mayo Clinic. Nov. 16, 2022.
• C-reactive protein (CRP), serum. Mayo Clinic Laboratories. https://www.mayocliniclabs.com/test-catalog/Clinical+and+Interpretive/82047. Accessed Nov. 15, 2022.
• Rheumatoid arthritis

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10.26: Hypothesis Test for a Population Mean (5 of 5)

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Learning Objectives

• Interpret the P-value as a conditional probability.

We finish our discussion of the hypothesis test for a population mean with a review of the meaning of the P-value, along with a review of type I and type II errors.

Review of the Meaning of the P-value

At this point, we assume you know how to use a P-value to make a decision in a hypothesis test. The logic is always the same. If we pick a level of significance (α), then we compare the P-value to α.

• If the P-value ≤ α, reject the null hypothesis. The data supports the alternative hypothesis.
• If the P-value > α, do not reject the null hypothesis. The data is not strong enough to support the alternative hypothesis.

In fact, we find that we treat these as “rules” and apply them without thinking about what the P-value means. So let’s pause here and review the meaning of the P-value, since it is the connection between probability and decision-making in inference.

Birth Weights in a Town

Let’s return to the familiar context of birth weights for babies in a town. Suppose that babies in the town had a mean birth weight of 3,500 grams in 2010. This year, a random sample of 50 babies has a mean weight of about 3,400 grams with a standard deviation of about 500 grams. Here is the distribution of birth weights in the sample.

Obviously, this sample weighs less on average than the population of babies in the town in 2010. A decrease in the town’s mean birth weight could indicate a decline in overall health of the town. But does this sample give strong evidence that the town’s mean birth weight is less than 3,500 grams this year?

We now know how to answer this question with a hypothesis test. Let’s use a significance level of 5%.

Let μ = mean birth weight in the town this year. The null hypothesis says there is “no change from 2010.”

• H 0 : μ < 3,500
• H a : μ = 3,500

Since the sample is large, we can conduct the T-test (without worrying about the shape of the distribution of birth weights for individual babies.)

Statistical software tells us the P-value is 0.082 = 8.2%. Since the P-value is greater than 0.05, we fail to reject the null hypothesis.

Our conclusion: This sample does not suggest that the mean birth weight this year is less than 3,500 grams ( P -value = 0.082). The sample from this year has a mean of 3,400 grams, which is 100 grams lower than the mean in 2010. But this difference is not statistically significant. It can be explained by the chance fluctuation we expect to see in random sampling.

What Does the P-Value of 0.082 Tell Us?

A simulation can help us understand the P-value. In a simulation, we assume that the population mean is 3,500 grams. This is the null hypothesis. We assume the null hypothesis is true and select 1,000 random samples from a population with a mean of 3,500 grams. The mean of the sampling distribution is at 3,500 (as predicted by the null hypothesis.) We see this in the simulated sampling distribution.

In the simulation, we can see that about 8.6% of the samples have a mean less than 3,400. Since probability is the relative frequency of an event in the long run, we say there is an 8.6% chance that a random sample of 500 babies has a mean less than 3,400 if the population mean is 3,500. We can see that the corresponding area to the left of T = −1.41 in the T-model (with df = 49) also gives us a good estimate of the probability. This area is the P-value, about 8.2%.

If we generalize this statement, we say the P-value is the probability that random samples have results more extreme than the data if the null hypothesis is true. (By more extreme, we mean further from value of the parameter, in the direction of the alternative hypothesis.) We can also describe the P-value in terms of T-scores. The P-value is the probability that the test statistic from a random sample has a value more extreme than that associated with the data if the null hypothesis is true.

What Does a P-Value Mean?

Do women who smoke run the risk of shorter pregnancy and premature birth? The mean pregnancy length is 266 days. We test the following hypotheses.

• H 0 : μ = 266
• H a : μ < 266

Suppose a random sample of 40 women who smoke during their pregnancy have a mean pregnancy length of 260 days with a standard deviation of 21 days. The P-value is 0.04.

What probability does the P-value of 0.04 describe? Label each of the following interpretations as valid or invalid.

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Review of Type I and Type II Errors

We know that statistical inference is based on probability, so there is always some chance of making a wrong decision. Recall that there are two types of wrong decisions that can be made in hypothesis testing. When we reject a null hypothesis that is true, we commit a type I error. When we fail to reject a null hypothesis that is false, we commit a type II error.

The following table summarizes the logic behind type I and type II errors.

It is possible to have some influence over the likelihoods of committing these errors. But decreasing the chance of a type I error increases the chance of a type II error. We have to decide which error is more serious for a given situation. Sometimes a type I error is more serious. Other times a type II error is more serious. Sometimes neither is serious.

Recall that if the null hypothesis is true, the probability of committing a type I error is α. Why is this? Well, when we choose a level of significance (α), we are choosing a benchmark for rejecting the null hypothesis. If the null hypothesis is true, then the probability that we will reject a true null hypothesis is α. So the smaller α is, the smaller the probability of a type I error.

It is more complicated to calculate the probability of a type II error. The best way to reduce the probability of a type II error is to increase the sample size. But once the sample size is set, larger values of α will decrease the probability of a type II error (while increasing the probability of a type I error).

General Guidelines for Choosing a Level of Significance

• If the consequences of a type I error are more serious, choose a small level of significance (α).
• If the consequences of a type II error are more serious, choose a larger level of significance (α). But remember that the level of significance is the probability of committing a type I error.
• In general, we pick the largest level of significance that we can tolerate as the chance of a type I error.

Let’s return to the investigation of the impact of smoking on pregnancy length.

Recap of the hypothesis test: The mean human pregnancy length is 266 days. We test the following hypotheses.

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Let’s Summarize

In this “Hypothesis Test for a Population Mean,” we looked at the four steps of a hypothesis test as they relate to a claim about a population mean.

Step 1: Determine the hypotheses.

• The hypotheses are claims about the population mean, µ.
• The null hypothesis is a hypothesis that the mean equals a specific value, µ 0 .

Step 2: Collect the data.

Since the hypothesis test is based on probability, random selection or assignment is essential in data production. Additionally, we need to check whether the t-model is a good fit for the sampling distribution of sample means. To use the t-model, the variable must be normally distributed in the population or the sample size must be more than 30. In practice, it is often impossible to verify that the variable is normally distributed in the population. If this is the case and the sample size is not more than 30, researchers often use the t-model if the sample is not strongly skewed and does not have outliers.

Step 3: Assess the evidence.

• If a t-model is appropriate, determine the t-test statistic for the data’s sample mean.
• Use the test statistic, together with the alternative hypothesis, to determine the P-value.
• The P-value is the probability of finding a random sample with a mean at least as extreme as our sample mean, assuming that the null hypothesis is true.
• As in all hypothesis tests, if the alternative hypothesis is greater than, the P-value is the area to the right of the test statistic. If the alternative hypothesis is less than, the P-value is the area to the left of the test statistic. If the alternative hypothesis is not equal to, the P-value is equal to double the tail area beyond the test statistic.

Step 4: Give the conclusion.

The logic of the hypothesis test is always the same. To state a conclusion about H 0 , we compare the P-value to the significance level, α.

• If P ≤ α, we reject H 0 . We conclude there is significant evidence in favor of H a .
• If P > α, we fail to reject H 0 . We conclude the sample does not provide significant evidence in favor of H a .
• We write the conclusion in the context of the research question. Our conclusion is usually a statement about the alternative hypothesis (we accept H a or fail to acceptH a ) and should include the P-value.

Other Hypothesis Testing Notes

• Remember that the P-value is the probability of seeing a sample mean at least as extreme as the one from the data if the null hypothesis is true. The probability is about the random sample; it is not a “chance” statement about the null or alternative hypothesis.
• If our test results in rejecting a null hypothesis that is actually true, then it is called a type I error.
• If our test results in failing to reject a null hypothesis that is actually false, then it is called a type II error.
• If rejecting a null hypothesis would be very expensive, controversial, or dangerous, then we really want to avoid a type I error. In this case, we would set a strict significance level (a small value of α, such as 0.01).
• Finally, remember the phrase “garbage in, garbage out.” If the data collection methods are poor, then the results of a hypothesis test are meaningless.

Contributors and Attributions

• Concepts in Statistics. Provided by : Open Learning Initiative. Located at : http://oli.cmu.edu . License : CC BY: Attribution