what is a hypothesis biology

Hypothesis n., plural: hypotheses [/haɪˈpɑːθəsɪs/] Definition: Testable scientific prediction

Table of Contents

What Is Hypothesis?

A scientific hypothesis is a foundational element of the scientific method . It’s a testable statement proposing a potential explanation for natural phenomena. The term hypothesis means “little theory” . A hypothesis is a short statement that can be tested and gives a possible reason for a phenomenon or a possible link between two variables . In the setting of scientific research, a hypothesis is a tentative explanation or statement that can be proven wrong and is used to guide experiments and empirical research.

It is an important part of the scientific method because it gives a basis for planning tests, gathering data, and judging evidence to see if it is true and could help us understand how natural things work. Several hypotheses can be tested in the real world, and the results of careful and systematic observation and analysis can be used to support, reject, or improve them.

Researchers and scientists often use the word hypothesis to refer to this educated guess . These hypotheses are firmly established based on scientific principles and the rigorous testing of new technology and experiments .

For example, in astrophysics, the Big Bang Theory is a working hypothesis that explains the origins of the universe and considers it as a natural phenomenon. It is among the most prominent scientific hypotheses in the field.

“The scientific method: steps, terms, and examples” by Scishow:

Biology definition: A hypothesis  is a supposition or tentative explanation for (a group of) phenomena, (a set of) facts, or a scientific inquiry that may be tested, verified or answered by further investigation or methodological experiment. It is like a scientific guess . It’s an idea or prediction that scientists make before they do experiments. They use it to guess what might happen and then test it to see if they were right. It’s like a smart guess that helps them learn new things. A scientific hypothesis that has been verified through scientific experiment and research may well be considered a scientific theory .

Etymology: The word “hypothesis” comes from the Greek word “hupothesis,” which means “a basis” or “a supposition.” It combines “hupo” (under) and “thesis” (placing). Synonym:   proposition; assumption; conjecture; postulate Compare:   theory See also: null hypothesis

Characteristics Of Hypothesis

A useful hypothesis must have the following qualities:

• It should never be written as a question.
• You should be able to test it in the real world to see if it’s right or wrong.
• It needs to be clear and exact.
• It should list the factors that will be used to figure out the relationship.
• It should only talk about one thing. You can make a theory in either a descriptive or form of relationship.
• It shouldn’t go against any natural rule that everyone knows is true. Verification will be done well with the tools and methods that are available.
• It should be written in as simple a way as possible so that everyone can understand it.
• It must explain what happened to make an answer necessary.
• It should be testable in a fair amount of time.
• It shouldn’t say different things.

Sources Of Hypothesis

Sources of hypothesis are:

• Patterns of similarity between the phenomenon under investigation and existing hypotheses.
• Insights derived from prior research, concurrent observations, and insights from opposing perspectives.
• The formulations are derived from accepted scientific theories and proposed by researchers.
• In research, it’s essential to consider hypothesis as different subject areas may require various hypotheses (plural form of hypothesis). Researchers also establish a significance level to determine the strength of evidence supporting a hypothesis.
• Individual cognitive processes also contribute to the formation of hypotheses.

One hypothesis is a tentative explanation for an observation or phenomenon. It is based on prior knowledge and understanding of the world, and it can be tested by gathering and analyzing data. Observed facts are the data that are collected to test a hypothesis. They can support or refute the hypothesis.

For example, the hypothesis that “eating more fruits and vegetables will improve your health” can be tested by gathering data on the health of people who eat different amounts of fruits and vegetables. If the people who eat more fruits and vegetables are healthier than those who eat less fruits and vegetables, then the hypothesis is supported.

Hypotheses are essential for scientific inquiry. They help scientists to focus their research, to design experiments, and to interpret their results. They are also essential for the development of scientific theories.

Types Of Hypothesis

In research, you typically encounter two types of hypothesis: the alternative hypothesis (which proposes a relationship between variables) and the null hypothesis (which suggests no relationship).

Simple Hypothesis

It illustrates the association between one dependent variable and one independent variable. For instance, if you consume more vegetables, you will lose weight more quickly. Here, increasing vegetable consumption is the independent variable, while weight loss is the dependent variable.

Complex Hypothesis

It exhibits the relationship between at least two dependent variables and at least two independent variables. Eating more vegetables and fruits results in weight loss, radiant skin, and a decreased risk of numerous diseases, including heart disease.

Directional Hypothesis

It shows that a researcher wants to reach a certain goal. The way the factors are related can also tell us about their nature. For example, four-year-old children who eat well over a time of five years have a higher IQ than children who don’t eat well. This shows what happened and how it happened.

Non-directional Hypothesis

When there is no theory involved, it is used. It is a statement that there is a connection between two variables, but it doesn’t say what that relationship is or which way it goes.

Null Hypothesis

It says something that goes against the theory. It’s a statement that says something is not true, and there is no link between the independent and dependent factors. “H 0 ” represents the null hypothesis.

Associative and Causal Hypothesis

When a change in one variable causes a change in the other variable, this is called the associative hypothesis . The causal hypothesis, on the other hand, says that there is a cause-and-effect relationship between two or more factors.

Examples Of Hypothesis

Examples of simple hypotheses:

• Students who consume breakfast before taking a math test will have a better overall performance than students who do not consume breakfast.
• Students who experience test anxiety before an English examination will get lower scores than students who do not experience test anxiety.
• Motorists who talk on the phone while driving will be more likely to make errors on a driving course than those who do not talk on the phone, is a statement that suggests that drivers who talk on the phone while driving are more likely to make mistakes.

Examples of a complex hypothesis:

• Individuals who consume a lot of sugar and don’t get much exercise are at an increased risk of developing depression.
• Younger people who are routinely exposed to green, outdoor areas have better subjective well-being than older adults who have limited exposure to green spaces, according to a new study.
• Increased levels of air pollution led to higher rates of respiratory illnesses, which in turn resulted in increased costs for healthcare for the affected communities.

Examples of Directional Hypothesis:

• The crop yield will go up a lot if the amount of fertilizer is increased.
• Patients who have surgery and are exposed to more stress will need more time to get better.
• Increasing the frequency of brand advertising on social media will lead to a significant increase in brand awareness among the target audience.

Examples of Non-Directional Hypothesis (or Two-Tailed Hypothesis):

• The test scores of two groups of students are very different from each other.
• There is a link between gender and being happy at work.
• There is a correlation between the amount of caffeine an individual consumes and the speed with which they react.

Examples of a null hypothesis:

• Children who receive a new reading intervention will have scores that are different than students who do not receive the intervention.
• The results of a memory recall test will not reveal any significant gap in performance between children and adults.
• There is not a significant relationship between the number of hours spent playing video games and academic performance.

Examples of Associative Hypothesis:

• There is a link between how many hours you spend studying and how well you do in school.
• Drinking sugary drinks is bad for your health as a whole.
• There is an association between socioeconomic status and access to quality healthcare services in urban neighborhoods.

Functions Of Hypothesis

The research issue can be understood better with the help of a hypothesis, which is why developing one is crucial. The following are some of the specific roles that a hypothesis plays: (Rashid, Apr 20, 2022)

• A hypothesis gives a study a point of concentration. It enlightens us as to the specific characteristics of a study subject we need to look into.
• It instructs us on what data to acquire as well as what data we should not collect, giving the study a focal point .
• The development of a hypothesis improves objectivity since it enables the establishment of a focal point.
• A hypothesis makes it possible for us to contribute to the development of the theory. Because of this, we are in a position to definitively determine what is true and what is untrue .

How will Hypothesis help in the Scientific Method?

• The scientific method begins with observation and inquiry about the natural world when formulating research questions. Researchers can refine their observations and queries into specific, testable research questions with the aid of hypothesis. They provide an investigation with a focused starting point.
• Hypothesis generate specific predictions regarding the expected outcomes of experiments or observations. These forecasts are founded on the researcher’s current knowledge of the subject. They elucidate what researchers anticipate observing if the hypothesis is true.
• Hypothesis direct the design of experiments and data collection techniques. Researchers can use them to determine which variables to measure or manipulate, which data to obtain, and how to conduct systematic and controlled research.
• Following the formulation of a hypothesis and the design of an experiment, researchers collect data through observation, measurement, or experimentation. The collected data is used to verify the hypothesis’s predictions.
• Hypothesis establish the criteria for evaluating experiment results. The observed data are compared to the predictions generated by the hypothesis. This analysis helps determine whether empirical evidence supports or refutes the hypothesis.
• The results of experiments or observations are used to derive conclusions regarding the hypothesis. If the data support the predictions, then the hypothesis is supported. If this is not the case, the hypothesis may be revised or rejected, leading to the formulation of new queries and hypothesis.
• The scientific approach is iterative, resulting in new hypothesis and research issues from previous trials. This cycle of hypothesis generation, testing, and refining drives scientific progress.

Importance Of Hypothesis

• Hypothesis are testable statements that enable scientists to determine if their predictions are accurate. This assessment is essential to the scientific method, which is based on empirical evidence.
• Hypothesis serve as the foundation for designing experiments or data collection techniques. They can be used by researchers to develop protocols and procedures that will produce meaningful results.
• Hypothesis hold scientists accountable for their assertions. They establish expectations for what the research should reveal and enable others to assess the validity of the findings.
• Hypothesis aid in identifying the most important variables of a study. The variables can then be measured, manipulated, or analyzed to determine their relationships.
• Hypothesis assist researchers in allocating their resources efficiently. They ensure that time, money, and effort are spent investigating specific concerns, as opposed to exploring random concepts.
• Testing hypothesis contribute to the scientific body of knowledge. Whether or not a hypothesis is supported, the results contribute to our understanding of a phenomenon.
• Hypothesis can result in the creation of theories. When supported by substantive evidence, hypothesis can serve as the foundation for larger theoretical frameworks that explain complex phenomena.
• Beyond scientific research, hypothesis play a role in the solution of problems in a variety of domains. They enable professionals to make educated assumptions about the causes of problems and to devise solutions.

Research Hypotheses: Did you know that a hypothesis refers to an educated guess or prediction about the outcome of a research study?

It’s like a roadmap guiding researchers towards their destination of knowledge. Just like a compass points north, a well-crafted hypothesis points the way to valuable discoveries in the world of science and inquiry.

Choose the best answer. 

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Further reading.

• RNA-DNA World Hypothesis
• BYJU’S. (2023). Hypothesis. Retrieved 01 Septermber 2023, from https://byjus.com/physics/hypothesis/#sources-of-hypothesis
• Collegedunia. (2023). Hypothesis. Retrieved 1 September 2023, from https://collegedunia.com/exams/hypothesis-science-articleid-7026#d
• Hussain, D. J. (2022). Hypothesis. Retrieved 01 September 2023, from https://mmhapu.ac.in/doc/eContent/Management/JamesHusain/Research%20Hypothesis%20-Meaning,%20Nature%20&%20Importance-Characteristics%20of%20Good%20%20Hypothesis%20Sem2.pdf
• Media, D. (2023). Hypothesis in the Scientific Method. Retrieved 01 September 2023, from https://www.verywellmind.com/what-is-a-hypothesis-2795239#toc-hypotheses-examples
• Rashid, M. H. A. (Apr 20, 2022). Research Methodology. Retrieved 01 September 2023, from https://limbd.org/hypothesis-definitions-functions-characteristics-types-errors-the-process-of-testing-a-hypothesis-hypotheses-in-qualitative-research/#:~:text=Functions%20of%20a%20Hypothesis%3A&text=Specifically%2C%20a%20hypothesis%20serves%20the,providing%20focus%20to%20the%20study.

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Last updated on September 8th, 2023

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How to Write a Great Hypothesis

Hypothesis Definition, Format, Examples, and Tips

Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

Amy Morin, LCSW, is a psychotherapist and international bestselling author. Her books, including "13 Things Mentally Strong People Don't Do," have been translated into more than 40 languages. Her TEDx talk,  "The Secret of Becoming Mentally Strong," is one of the most viewed talks of all time.

Verywell / Alex Dos Diaz

• The Scientific Method

Hypothesis Format

Falsifiability of a hypothesis.

• Operationalization

Hypothesis Types

Hypotheses examples.

• Collecting Data

A hypothesis is a tentative statement about the relationship between two or more variables. It is a specific, testable prediction about what you expect to happen in a study. It is a preliminary answer to your question that helps guide the research process.

Consider a study designed to examine the relationship between sleep deprivation and test performance. The hypothesis might be: "This study is designed to assess the hypothesis that sleep-deprived people will perform worse on a test than individuals who are not sleep-deprived."

At a Glance

A hypothesis is crucial to scientific research because it offers a clear direction for what the researchers are looking to find. This allows them to design experiments to test their predictions and add to our scientific knowledge about the world. This article explores how a hypothesis is used in psychology research, how to write a good hypothesis, and the different types of hypotheses you might use.

The Hypothesis in the Scientific Method

In the scientific method , whether it involves research in psychology, biology, or some other area, a hypothesis represents what the researchers think will happen in an experiment. The scientific method involves the following steps:

• Forming a question
• Performing background research
• Creating a hypothesis
• Designing an experiment
• Collecting data
• Analyzing the results
• Drawing conclusions
• Communicating the results

The hypothesis is a prediction, but it involves more than a guess. Most of the time, the hypothesis begins with a question which is then explored through background research. At this point, researchers then begin to develop a testable hypothesis.

Unless you are creating an exploratory study, your hypothesis should always explain what you  expect  to happen.

In a study exploring the effects of a particular drug, the hypothesis might be that researchers expect the drug to have some type of effect on the symptoms of a specific illness. In psychology, the hypothesis might focus on how a certain aspect of the environment might influence a particular behavior.

Remember, a hypothesis does not have to be correct. While the hypothesis predicts what the researchers expect to see, the goal of the research is to determine whether this guess is right or wrong. When conducting an experiment, researchers might explore numerous factors to determine which ones might contribute to the ultimate outcome.

In many cases, researchers may find that the results of an experiment  do not  support the original hypothesis. When writing up these results, the researchers might suggest other options that should be explored in future studies.

In many cases, researchers might draw a hypothesis from a specific theory or build on previous research. For example, prior research has shown that stress can impact the immune system. So a researcher might hypothesize: "People with high-stress levels will be more likely to contract a common cold after being exposed to the virus than people who have low-stress levels."

In other instances, researchers might look at commonly held beliefs or folk wisdom. "Birds of a feather flock together" is one example of folk adage that a psychologist might try to investigate. The researcher might pose a specific hypothesis that "People tend to select romantic partners who are similar to them in interests and educational level."

Elements of a Good Hypothesis

So how do you write a good hypothesis? When trying to come up with a hypothesis for your research or experiments, ask yourself the following questions:

• Is your hypothesis based on your research on a topic?
• Can your hypothesis be tested?
• Does your hypothesis include independent and dependent variables?

Before you come up with a specific hypothesis, spend some time doing background research. Once you have completed a literature review, start thinking about potential questions you still have. Pay attention to the discussion section in the  journal articles you read . Many authors will suggest questions that still need to be explored.

How to Formulate a Good Hypothesis

To form a hypothesis, you should take these steps:

• Collect as many observations about a topic or problem as you can.
• Evaluate these observations and look for possible causes of the problem.
• Create a list of possible explanations that you might want to explore.
• After you have developed some possible hypotheses, think of ways that you could confirm or disprove each hypothesis through experimentation. This is known as falsifiability.

In the scientific method ,  falsifiability is an important part of any valid hypothesis. In order to test a claim scientifically, it must be possible that the claim could be proven false.

Students sometimes confuse the idea of falsifiability with the idea that it means that something is false, which is not the case. What falsifiability means is that  if  something was false, then it is possible to demonstrate that it is false.

One of the hallmarks of pseudoscience is that it makes claims that cannot be refuted or proven false.

The Importance of Operational Definitions

A variable is a factor or element that can be changed and manipulated in ways that are observable and measurable. However, the researcher must also define how the variable will be manipulated and measured in the study.

Operational definitions are specific definitions for all relevant factors in a study. This process helps make vague or ambiguous concepts detailed and measurable.

For example, a researcher might operationally define the variable " test anxiety " as the results of a self-report measure of anxiety experienced during an exam. A "study habits" variable might be defined by the amount of studying that actually occurs as measured by time.

These precise descriptions are important because many things can be measured in various ways. Clearly defining these variables and how they are measured helps ensure that other researchers can replicate your results.

Replicability

One of the basic principles of any type of scientific research is that the results must be replicable.

Replication means repeating an experiment in the same way to produce the same results. By clearly detailing the specifics of how the variables were measured and manipulated, other researchers can better understand the results and repeat the study if needed.

Some variables are more difficult than others to define. For example, how would you operationally define a variable such as aggression ? For obvious ethical reasons, researchers cannot create a situation in which a person behaves aggressively toward others.

To measure this variable, the researcher must devise a measurement that assesses aggressive behavior without harming others. The researcher might utilize a simulated task to measure aggressiveness in this situation.

Hypothesis Checklist

• Does your hypothesis focus on something that you can actually test?
• Does your hypothesis include both an independent and dependent variable?
• Can you manipulate the variables?
• Can your hypothesis be tested without violating ethical standards?

The hypothesis you use will depend on what you are investigating and hoping to find. Some of the main types of hypotheses that you might use include:

• Simple hypothesis : This type of hypothesis suggests there is a relationship between one independent variable and one dependent variable.
• Complex hypothesis : This type suggests a relationship between three or more variables, such as two independent and dependent variables.
• Null hypothesis : This hypothesis suggests no relationship exists between two or more variables.
• Alternative hypothesis : This hypothesis states the opposite of the null hypothesis.
• Statistical hypothesis : This hypothesis uses statistical analysis to evaluate a representative population sample and then generalizes the findings to the larger group.
• Logical hypothesis : This hypothesis assumes a relationship between variables without collecting data or evidence.

A hypothesis often follows a basic format of "If {this happens} then {this will happen}." One way to structure your hypothesis is to describe what will happen to the  dependent variable  if you change the  independent variable .

The basic format might be: "If {these changes are made to a certain independent variable}, then we will observe {a change in a specific dependent variable}."

A few examples of simple hypotheses:

• "Students who eat breakfast will perform better on a math exam than students who do not eat breakfast."
• "Students who experience test anxiety before an English exam will get lower scores than students who do not experience test anxiety."​
• "Motorists who talk on the phone while driving will be more likely to make errors on a driving course than those who do not talk on the phone."
• "Children who receive a new reading intervention will have higher reading scores than students who do not receive the intervention."

Examples of a complex hypothesis include:

• "People with high-sugar diets and sedentary activity levels are more likely to develop depression."
• "Younger people who are regularly exposed to green, outdoor areas have better subjective well-being than older adults who have limited exposure to green spaces."

Examples of a null hypothesis include:

• "There is no difference in anxiety levels between people who take St. John's wort supplements and those who do not."
• "There is no difference in scores on a memory recall task between children and adults."
• "There is no difference in aggression levels between children who play first-person shooter games and those who do not."

Examples of an alternative hypothesis:

• "People who take St. John's wort supplements will have less anxiety than those who do not."
• "Adults will perform better on a memory task than children."
• "Children who play first-person shooter games will show higher levels of aggression than children who do not." 

Collecting Data on Your Hypothesis

Once a researcher has formed a testable hypothesis, the next step is to select a research design and start collecting data. The research method depends largely on exactly what they are studying. There are two basic types of research methods: descriptive research and experimental research.

Descriptive Research Methods

Descriptive research such as  case studies ,  naturalistic observations , and surveys are often used when  conducting an experiment is difficult or impossible. These methods are best used to describe different aspects of a behavior or psychological phenomenon.

Once a researcher has collected data using descriptive methods, a  correlational study  can examine how the variables are related. This research method might be used to investigate a hypothesis that is difficult to test experimentally.

Experimental Research Methods

Experimental methods  are used to demonstrate causal relationships between variables. In an experiment, the researcher systematically manipulates a variable of interest (known as the independent variable) and measures the effect on another variable (known as the dependent variable).

Unlike correlational studies, which can only be used to determine if there is a relationship between two variables, experimental methods can be used to determine the actual nature of the relationship—whether changes in one variable actually  cause  another to change.

The hypothesis is a critical part of any scientific exploration. It represents what researchers expect to find in a study or experiment. In situations where the hypothesis is unsupported by the research, the research still has value. Such research helps us better understand how different aspects of the natural world relate to one another. It also helps us develop new hypotheses that can then be tested in the future.

Thompson WH, Skau S. On the scope of scientific hypotheses .  R Soc Open Sci . 2023;10(8):230607. doi:10.1098/rsos.230607

Taran S, Adhikari NKJ, Fan E. Falsifiability in medicine: what clinicians can learn from Karl Popper [published correction appears in Intensive Care Med. 2021 Jun 17;:].  Intensive Care Med . 2021;47(9):1054-1056. doi:10.1007/s00134-021-06432-z

Eyler AA. Research Methods for Public Health . 1st ed. Springer Publishing Company; 2020. doi:10.1891/9780826182067.0004

Nosek BA, Errington TM. What is replication ?  PLoS Biol . 2020;18(3):e3000691. doi:10.1371/journal.pbio.3000691

Aggarwal R, Ranganathan P. Study designs: Part 2 - Descriptive studies .  Perspect Clin Res . 2019;10(1):34-36. doi:10.4103/picr.PICR_154_18

Nevid J. Psychology: Concepts and Applications. Wadworth, 2013.

By Kendra Cherry, MSEd Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

What is a scientific hypothesis?

It's the initial building block in the scientific method.

Hypothesis basics

What makes a hypothesis testable.

• Types of hypotheses
• Hypothesis versus theory

Additional resources

Bibliography.

A scientific hypothesis is a tentative, testable explanation for a phenomenon in the natural world. It's the initial building block in the scientific method . Many describe it as an "educated guess" based on prior knowledge and observation. While this is true, a hypothesis is more informed than a guess. While an "educated guess" suggests a random prediction based on a person's expertise, developing a hypothesis requires active observation and background research. 

The basic idea of a hypothesis is that there is no predetermined outcome. For a solution to be termed a scientific hypothesis, it has to be an idea that can be supported or refuted through carefully crafted experimentation or observation. This concept, called falsifiability and testability, was advanced in the mid-20th century by Austrian-British philosopher Karl Popper in his famous book "The Logic of Scientific Discovery" (Routledge, 1959).

A key function of a hypothesis is to derive predictions about the results of future experiments and then perform those experiments to see whether they support the predictions.

A hypothesis is usually written in the form of an if-then statement, which gives a possibility (if) and explains what may happen because of the possibility (then). The statement could also include "may," according to California State University, Bakersfield .

Here are some examples of hypothesis statements:

• If garlic repels fleas, then a dog that is given garlic every day will not get fleas.
• If sugar causes cavities, then people who eat a lot of candy may be more prone to cavities.
• If ultraviolet light can damage the eyes, then maybe this light can cause blindness.

A useful hypothesis should be testable and falsifiable. That means that it should be possible to prove it wrong. A theory that can't be proved wrong is nonscientific, according to Karl Popper's 1963 book " Conjectures and Refutations ."

An example of an untestable statement is, "Dogs are better than cats." That's because the definition of "better" is vague and subjective. However, an untestable statement can be reworded to make it testable. For example, the previous statement could be changed to this: "Owning a dog is associated with higher levels of physical fitness than owning a cat." With this statement, the researcher can take measures of physical fitness from dog and cat owners and compare the two.

Types of scientific hypotheses

In an experiment, researchers generally state their hypotheses in two ways. The null hypothesis predicts that there will be no relationship between the variables tested, or no difference between the experimental groups. The alternative hypothesis predicts the opposite: that there will be a difference between the experimental groups. This is usually the hypothesis scientists are most interested in, according to the University of Miami .

For example, a null hypothesis might state, "There will be no difference in the rate of muscle growth between people who take a protein supplement and people who don't." The alternative hypothesis would state, "There will be a difference in the rate of muscle growth between people who take a protein supplement and people who don't."

If the results of the experiment show a relationship between the variables, then the null hypothesis has been rejected in favor of the alternative hypothesis, according to the book " Research Methods in Psychology " (​​BCcampus, 2015). 

There are other ways to describe an alternative hypothesis. The alternative hypothesis above does not specify a direction of the effect, only that there will be a difference between the two groups. That type of prediction is called a two-tailed hypothesis. If a hypothesis specifies a certain direction — for example, that people who take a protein supplement will gain more muscle than people who don't — it is called a one-tailed hypothesis, according to William M. K. Trochim , a professor of Policy Analysis and Management at Cornell University.

Sometimes, errors take place during an experiment. These errors can happen in one of two ways. A type I error is when the null hypothesis is rejected when it is true. This is also known as a false positive. A type II error occurs when the null hypothesis is not rejected when it is false. This is also known as a false negative, according to the University of California, Berkeley . 

A hypothesis can be rejected or modified, but it can never be proved correct 100% of the time. For example, a scientist can form a hypothesis stating that if a certain type of tomato has a gene for red pigment, that type of tomato will be red. During research, the scientist then finds that each tomato of this type is red. Though the findings confirm the hypothesis, there may be a tomato of that type somewhere in the world that isn't red. Thus, the hypothesis is true, but it may not be true 100% of the time.

Scientific theory vs. scientific hypothesis

The best hypotheses are simple. They deal with a relatively narrow set of phenomena. But theories are broader; they generally combine multiple hypotheses into a general explanation for a wide range of phenomena, according to the University of California, Berkeley . For example, a hypothesis might state, "If animals adapt to suit their environments, then birds that live on islands with lots of seeds to eat will have differently shaped beaks than birds that live on islands with lots of insects to eat." After testing many hypotheses like these, Charles Darwin formulated an overarching theory: the theory of evolution by natural selection.

"Theories are the ways that we make sense of what we observe in the natural world," Tanner said. "Theories are structures of ideas that explain and interpret facts." 

• Read more about writing a hypothesis, from the American Medical Writers Association.
• Find out why a hypothesis isn't always necessary in science, from The American Biology Teacher.
• Learn about null and alternative hypotheses, from Prof. Essa on YouTube .

Encyclopedia Britannica. Scientific Hypothesis. Jan. 13, 2022. https://www.britannica.com/science/scientific-hypothesis

Karl Popper, "The Logic of Scientific Discovery," Routledge, 1959.

California State University, Bakersfield, "Formatting a testable hypothesis." https://www.csub.edu/~ddodenhoff/Bio100/Bio100sp04/formattingahypothesis.htm  

Karl Popper, "Conjectures and Refutations," Routledge, 1963.

Price, P., Jhangiani, R., & Chiang, I., "Research Methods of Psychology — 2nd Canadian Edition," BCcampus, 2015.‌

University of Miami, "The Scientific Method" http://www.bio.miami.edu/dana/161/evolution/161app1_scimethod.pdf  

William M.K. Trochim, "Research Methods Knowledge Base," https://conjointly.com/kb/hypotheses-explained/  

University of California, Berkeley, "Multiple Hypothesis Testing and False Discovery Rate" https://www.stat.berkeley.edu/~hhuang/STAT141/Lecture-FDR.pdf  

University of California, Berkeley, "Science at multiple levels" https://undsci.berkeley.edu/article/0_0_0/howscienceworks_19

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Module 1: Introduction to Biology

Experiments and hypotheses, learning outcomes.

• Form a hypothesis and use it to design a scientific experiment

Now we’ll focus on the methods of scientific inquiry. Science often involves making observations and developing hypotheses. Experiments and further observations are often used to test the hypotheses.

A scientific experiment is a carefully organized procedure in which the scientist intervenes in a system to change something, then observes the result of the change. Scientific inquiry often involves doing experiments, though not always. For example, a scientist studying the mating behaviors of ladybugs might begin with detailed observations of ladybugs mating in their natural habitats. While this research may not be experimental, it is scientific: it involves careful and verifiable observation of the natural world. The same scientist might then treat some of the ladybugs with a hormone hypothesized to trigger mating and observe whether these ladybugs mated sooner or more often than untreated ones. This would qualify as an experiment because the scientist is now making a change in the system and observing the effects.

Forming a Hypothesis

When conducting scientific experiments, researchers develop hypotheses to guide experimental design. A hypothesis is a suggested explanation that is both testable and falsifiable. You must be able to test your hypothesis through observations and research, and it must be possible to prove your hypothesis false.

For example, Michael observes that maple trees lose their leaves in the fall. He might then propose a possible explanation for this observation: “cold weather causes maple trees to lose their leaves in the fall.” This statement is testable. He could grow maple trees in a warm enclosed environment such as a greenhouse and see if their leaves still dropped in the fall. The hypothesis is also falsifiable. If the leaves still dropped in the warm environment, then clearly temperature was not the main factor in causing maple leaves to drop in autumn.

In the Try It below, you can practice recognizing scientific hypotheses. As you consider each statement, try to think as a scientist would: can I test this hypothesis with observations or experiments? Is the statement falsifiable? If the answer to either of these questions is “no,” the statement is not a valid scientific hypothesis.

Practice Questions

Determine whether each following statement is a scientific hypothesis.

Air pollution from automobile exhaust can trigger symptoms in people with asthma.

• No. This statement is not testable or falsifiable.
• No. This statement is not testable.
• No. This statement is not falsifiable.
• Yes. This statement is testable and falsifiable.

Natural disasters, such as tornadoes, are punishments for bad thoughts and behaviors.

a: No. This statement is not testable or falsifiable. “Bad thoughts and behaviors” are excessively vague and subjective variables that would be impossible to measure or agree upon in a reliable way. The statement might be “falsifiable” if you came up with a counterexample: a “wicked” place that was not punished by a natural disaster. But some would question whether the people in that place were really wicked, and others would continue to predict that a natural disaster was bound to strike that place at some point. There is no reason to suspect that people’s immoral behavior affects the weather unless you bring up the intervention of a supernatural being, making this idea even harder to test.

Testing a Vaccine

Let’s examine the scientific process by discussing an actual scientific experiment conducted by researchers at the University of Washington. These researchers investigated whether a vaccine may reduce the incidence of the human papillomavirus (HPV). The experimental process and results were published in an article titled, “ A controlled trial of a human papillomavirus type 16 vaccine .”

Preliminary observations made by the researchers who conducted the HPV experiment are listed below:

• Human papillomavirus (HPV) is the most common sexually transmitted virus in the United States.
• There are about 40 different types of HPV. A significant number of people that have HPV are unaware of it because many of these viruses cause no symptoms.
• Some types of HPV can cause cervical cancer.
• About 4,000 women a year die of cervical cancer in the United States.

Practice Question

Researchers have developed a potential vaccine against HPV and want to test it. What is the first testable hypothesis that the researchers should study?

• HPV causes cervical cancer.
• People should not have unprotected sex with many partners.
• People who get the vaccine will not get HPV.
• The HPV vaccine will protect people against cancer.

Experimental Design

You’ve successfully identified a hypothesis for the University of Washington’s study on HPV: People who get the HPV vaccine will not get HPV.

The next step is to design an experiment that will test this hypothesis. There are several important factors to consider when designing a scientific experiment. First, scientific experiments must have an experimental group. This is the group that receives the experimental treatment necessary to address the hypothesis.

The experimental group receives the vaccine, but how can we know if the vaccine made a difference? Many things may change HPV infection rates in a group of people over time. To clearly show that the vaccine was effective in helping the experimental group, we need to include in our study an otherwise similar control group that does not get the treatment. We can then compare the two groups and determine if the vaccine made a difference. The control group shows us what happens in the absence of the factor under study.

However, the control group cannot get “nothing.” Instead, the control group often receives a placebo. A placebo is a procedure that has no expected therapeutic effect—such as giving a person a sugar pill or a shot containing only plain saline solution with no drug. Scientific studies have shown that the “placebo effect” can alter experimental results because when individuals are told that they are or are not being treated, this knowledge can alter their actions or their emotions, which can then alter the results of the experiment.

Moreover, if the doctor knows which group a patient is in, this can also influence the results of the experiment. Without saying so directly, the doctor may show—through body language or other subtle cues—their views about whether the patient is likely to get well. These errors can then alter the patient’s experience and change the results of the experiment. Therefore, many clinical studies are “double blind.” In these studies, neither the doctor nor the patient knows which group the patient is in until all experimental results have been collected.

Both placebo treatments and double-blind procedures are designed to prevent bias. Bias is any systematic error that makes a particular experimental outcome more or less likely. Errors can happen in any experiment: people make mistakes in measurement, instruments fail, computer glitches can alter data. But most such errors are random and don’t favor one outcome over another. Patients’ belief in a treatment can make it more likely to appear to “work.” Placebos and double-blind procedures are used to level the playing field so that both groups of study subjects are treated equally and share similar beliefs about their treatment.

The scientists who are researching the effectiveness of the HPV vaccine will test their hypothesis by separating 2,392 young women into two groups: the control group and the experimental group. Answer the following questions about these two groups.

• This group is given a placebo.
• This group is deliberately infected with HPV.
• This group is given nothing.
• This group is given the HPV vaccine.
• a: This group is given a placebo. A placebo will be a shot, just like the HPV vaccine, but it will have no active ingredient. It may change peoples’ thinking or behavior to have such a shot given to them, but it will not stimulate the immune systems of the subjects in the same way as predicted for the vaccine itself.
• d: This group is given the HPV vaccine. The experimental group will receive the HPV vaccine and researchers will then be able to see if it works, when compared to the control group.

Experimental Variables

A variable is a characteristic of a subject (in this case, of a person in the study) that can vary over time or among individuals. Sometimes a variable takes the form of a category, such as male or female; often a variable can be measured precisely, such as body height. Ideally, only one variable is different between the control group and the experimental group in a scientific experiment. Otherwise, the researchers will not be able to determine which variable caused any differences seen in the results. For example, imagine that the people in the control group were, on average, much more sexually active than the people in the experimental group. If, at the end of the experiment, the control group had a higher rate of HPV infection, could you confidently determine why? Maybe the experimental subjects were protected by the vaccine, but maybe they were protected by their low level of sexual contact.

To avoid this situation, experimenters make sure that their subject groups are as similar as possible in all variables except for the variable that is being tested in the experiment. This variable, or factor, will be deliberately changed in the experimental group. The one variable that is different between the two groups is called the independent variable. An independent variable is known or hypothesized to cause some outcome. Imagine an educational researcher investigating the effectiveness of a new teaching strategy in a classroom. The experimental group receives the new teaching strategy, while the control group receives the traditional strategy. It is the teaching strategy that is the independent variable in this scenario. In an experiment, the independent variable is the variable that the scientist deliberately changes or imposes on the subjects.

Dependent variables are known or hypothesized consequences; they are the effects that result from changes or differences in an independent variable. In an experiment, the dependent variables are those that the scientist measures before, during, and particularly at the end of the experiment to see if they have changed as expected. The dependent variable must be stated so that it is clear how it will be observed or measured. Rather than comparing “learning” among students (which is a vague and difficult to measure concept), an educational researcher might choose to compare test scores, which are very specific and easy to measure.

In any real-world example, many, many variables MIGHT affect the outcome of an experiment, yet only one or a few independent variables can be tested. Other variables must be kept as similar as possible between the study groups and are called control variables . For our educational research example, if the control group consisted only of people between the ages of 18 and 20 and the experimental group contained people between the ages of 30 and 35, we would not know if it was the teaching strategy or the students’ ages that played a larger role in the results. To avoid this problem, a good study will be set up so that each group contains students with a similar age profile. In a well-designed educational research study, student age will be a controlled variable, along with other possibly important factors like gender, past educational achievement, and pre-existing knowledge of the subject area.

What is the independent variable in this experiment?

• Sex (all of the subjects will be female)
• Presence or absence of the HPV vaccine
• Presence or absence of HPV (the virus)

List three control variables other than age.

What is the dependent variable in this experiment?

• Sex (male or female)
• Rates of HPV infection
• Age (years)
• Revision and adaptation. Authored by : Shelli Carter and Lumen Learning. Provided by : Lumen Learning. License : CC BY-NC-SA: Attribution-NonCommercial-ShareAlike
• Scientific Inquiry. Provided by : Open Learning Initiative. Located at : https://oli.cmu.edu/jcourse/workbook/activity/page?context=434a5c2680020ca6017c03488572e0f8 . Project : Introduction to Biology (Open + Free). License : CC BY-NC-SA: Attribution-NonCommercial-ShareAlike

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Three Famous Hypotheses and How They Were Tested

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Key Takeaways

• Ivan Pavlov's experiment demonstrated conditioned responses in dogs.
• Pavlov's work exemplifies the scientific method, starting with a hypothesis about conditioned responses and testing it through controlled experiments.
• Pavlov's findings not only advanced an understanding of animal physiology but also laid foundational principles for behaviorism, a major school of thought in psychology that emphasizes the study of observable behaviors.

Coho salmon ( Oncorhynchus kisutch ) are amazing fish. Indigenous to the Pacific Northwest, they begin their lives in freshwater streams and then relocate to the open ocean. But when a Coho salmon reaches breeding age, it'll return to the waterway of its birth , sometimes traveling 400 miles (644 kilometers) to get there.

Enter the late Arthur Davis Hasler. While an ecologist and biologist at the University of Wisconsin, he was intrigued by the question of how these creatures find their home streams. And in 1960, he used a Hypothesis-Presentation.pdf">basic tenet of science — the hypothesis — to find out.

So what is a hypothesis? A hypothesis is a tentative, testable explanation for an observed phenomenon in nature. Hypotheses are narrow in scope — unlike theories , which cover a broad range of observable phenomena and draw from many different lines of evidence. Meanwhile, a prediction is a result you'd expect to get if your hypothesis or theory is accurate.

So back to 1960 and Hasler and those salmon. One unverified idea was that Coho salmon used eyesight to locate their home streams. Hasler set out to test this notion (or hypothesis). First, he rounded up several fish who'd already returned to their native streams. Next, he blindfolded some of the captives — but not all of them — before dumping his salmon into a faraway stretch of water. If the eyesight hypothesis was correct, then Hasler could expect fewer of the blindfolded fish to return to their home streams.

Things didn't work out that way. The fish without blindfolds came back at the same rate as their blindfolded counterparts. (Other experiments demonstrated that smell, and not sight, is the key to the species' homing ability.)

Although Hasler's blindfold hypothesis was disproven, others have fared better. Today, we're looking at three of the best-known experiments in history — and the hypotheses they tested.

Ivan Pavlov and His Dogs (1903-1935)

Isaac newton's radiant prisms (1665), robert paine's revealing starfish (1963-1969).

The Hypothesis : If dogs are susceptible to conditioned responses (drooling), then a dog who is regularly exposed to the same neutral stimulus (metronome/bell) before it receives food will associate this neutral stimulus with the act of eating. Eventually, the dog should begin to drool at a predictable rate when it encounters said stimulus — even before any actual food is offered.

The Experiment : A Nobel Prize-winner and outspoken critic of Soviet communism, Ivan Pavlov is synonymous with man's best friend . In 1903, the Russian-born scientist kicked off a decades-long series of experiments involving dogs and conditioned responses .

Offer a plate of food to a hungry dog and it'll salivate. In this context, the stimulus (the food) will automatically trigger a particular response (the drooling). The latter is an innate, unlearned reaction to the former.

By contrast, the rhythmic sound of a metronome or bell is a neutral stimulus. To a dog, the noise has no inherent meaning and if the animal has never heard it before, the sound won't provoke an instinctive reaction. But the sight of food sure will .

So when Pavlov and his lab assistants played the sound of the metronome/bell before feeding sessions, the researchers conditioned test dogs to mentally link metronomes/bells with mealtime. Due to repeated exposure, the noise alone started to make the dogs' mouths water before they were given food.

According to " Ivan Pavlov: A Russian Life in Science " by biographer Daniel P. Todes, Pavlov's big innovation here was his discovery that he could quantify the reaction of each pooch by measuring the amount of saliva it generated. Every canine predictably drooled at its own consistent rate when he or she encountered a personalized (and artificial) food-related cue.

Pavlov and his assistants used conditioned responses to look at other hypotheses about animal physiology, as well. In one notable experiment, a dog was tested on its ability to tell time . This particular pooch always received food when it heard a metronome click at the rate of 60 strokes per minute. But it never got any food after listening to a slower, 40-strokes-per-minute beat. Lo and behold, Pavlov's animal began to salivate in response to the faster rhythm — but not the slower one . So clearly, it could tell the two rhythmic beats apart.

The Verdict : With the right conditioning — and lots of patience — you can make a hungry dog respond to neutral stimuli by salivating on cue in a way that's both predictable and scientifically quantifiable.

The Hypothesis : If white sunlight is a mixture of all the colors in the visible spectrum — and these travel at varying wavelengths — then each color will refract at a different angle when a beam of sunlight passes through a glass prism.

The Experiments : Color was a scientific mystery before Isaac Newton came along. During the summer of 1665, he started experimenting with glass prisms from the safety of a darkened room in Cambridge, England.

He cut a quarter-inch (0.63-centimeter) circular hole into one of the window shutters, allowing a single beam of sunlight to enter the place. When Newton held up a prism to this ray, an oblong patch of multicolored light was projected onto the opposite wall.

This contained segregated layers of red, orange, yellow, green, blue, indigo and violet light. From top to bottom, this patch measured 13.5 inches (33.65 centimeters) tall, yet it was only 2.6 inches (6.6 centimeters) across.

Newton deduced that these vibrant colors had been hiding within the sunlight itself, but the prism bent (or "refracted") them at different angles, which separated the colors out.

Still, he wasn't 100 percent sure. So Newton replicated the experiment with one small change. This time, he took a second prism and had it intercept the rainbow-like patch of light. Once the refracted colors entered the new prism, they recombined into a circular white sunbeam. In other words, Newton took a ray of white light, broke it apart into a bunch of different colors and then reassembled it. What a neat party trick!

The Verdict : Sunlight really is a blend of all the colors in the rainbow — and yes, these can be individually separated via light refraction.

The Hypothesis : If predators limit the populations of the organisms they attack, then we'd expect the prey species to become more common after the eradication of a major predator.

The Experiment : Meet Pisaster ochraceus , also known as the purple sea star (or the purple starfish if you prefer).

Using an extendable stomach , the creature feeds on mussels, limpets, barnacles, snails and other hapless victims. On some seaside rocks (and tidal pools) along the coast of Washington state, this starfish is the apex predator.

The animal made Robert Paine a scientific celebrity. An ecologist by trade, Paine was fascinated by the environmental roles of top predators. In June 1963, he kicked off an ambitious experiment along Washington state's Mukkaw Bay. For years on end, Paine kept a rocky section of this shoreline completely starfish-free.

It was hard work. Paine had to regularly pry wayward sea stars off "his" outcrop — sometimes with a crowbar. Then he'd chuck them into the ocean.

Before the experiment, Paine observed 15 different species of animals and algae inhabiting the area he decided to test. By June 1964 — one year after his starfish purge started — that number had dropped to eight .

Unchecked by purple sea stars, the barnacle population skyrocketed. Subsequently, these were replaced by California mussels , which came to dominate the terrain. By anchoring themselves to rocks in great numbers, the mussels edged out other life-forms. That made the outcrop uninhabitable to most former residents: Even sponges, anemones and algae — organisms that Pisaster ochraceus doesn't eat — were largely evicted.

All those species continued to thrive on another piece of shoreline that Paine left untouched. Later experiments convinced him that Pisaster ochraceus is a " keystone species ," a creature who exerts disproportionate influence over its environment. Eliminate the keystone and the whole system gets disheveled.

The Verdict : Apex predators don't just affect the animals that they hunt. Removing a top predator sets off a chain reaction that can fundamentally transform an entire ecosystem.

Contrary to popular belief, Pavlov almost never used bells in his dog experiments. Instead, he preferred metronomes, buzzers, harmoniums and electric shocks.

Frequently Asked Questions

How can a hypothesis become a theory, what's the difference between a hypothesis and a prediction.

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A hypothesis is an explanation that is proposed for a phenomenon. Formulating a hypothesis is a step of the scientific method .

Alternate Spellings: plural: hypotheses

Examples: Upon observing that a lake appears blue under a blue sky, you might propose the hypothesis that the lake is blue because it is reflecting the sky. One alternate hypothesis would be that the lake is blue because water is blue.

Hypothesis Versus Theory

Although in common usage the terms hypothesis and theory are used interchangeably, the two words mean something different from each other in science. Like a hypothesis, a theory is testable and may be used to make predictions. However, a theory has been tested using the scientific method many times. Testing a hypothesis may, over time, lead to the formulation of a theory.

• Theory Definition in Science
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Scientific Method: Step 3: HYPOTHESIS

• Step 1: QUESTION
• Step 2: RESEARCH
• Step 3: HYPOTHESIS
• Step 4: EXPERIMENT
• Step 5: DATA
• Step 6: CONCLUSION

Step 3: State your hypothesis

Now it's time to state your hypothesis . The hypothesis is an educated guess as to what will happen during your experiment. 

The hypothesis is often written using the words "IF" and "THEN." For example, " If I do not study, then I will fail the test." The "if' and "then" statements reflect your independent and dependent variables . 

The hypothesis should relate back to your original question and must be testable .

A word about variables...

Your experiment will include variables to measure and to explain any cause and effect. Below you will find some useful links describing the different types of variables.

• "What are independent and dependent variables" NCES
• [VIDEO] Biology: Independent vs. Dependent Variables (Nucleus Medical Media) Video explaining independent and dependent variables, with examples.

Resource Links

• What is and How to Write a Good Hypothesis in Research? (Elsevier)
• Hypothesis brochure from Penn State/Berks

• << Previous: Step 2: RESEARCH
• Next: Step 4: EXPERIMENT >>
• Last Updated: Aug 26, 2024 10:04 AM
• URL: https://harford.libguides.com/scientific_method

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Hypothesis | Definition, Meaning and Examples

Hypothesis is a hypothesis is fundamental concept in the world of research and statistics. It is a testable statement that explains what is happening or observed. It proposes the relation between the various participating variables.

Hypothesis is also called Theory, Thesis, Guess, Assumption, or Suggestion . Hypothesis creates a structure that guides the search for knowledge.

In this article, we will learn what hypothesis is, its characteristics, types, and examples. We will also learn how hypothesis helps in scientific research.

Table of Content

What is Hypothesis?

Characteristics of hypothesis, sources of hypothesis, types of hypothesis, functions of hypothesis, how hypothesis help in scientific research.

Hypothesis is a suggested idea or an educated guess or a proposed explanation made based on limited evidence, serving as a starting point for further study. They are meant to lead to more investigation.

It's mainly a smart guess or suggested answer to a problem that can be checked through study and trial. In science work, we make guesses called hypotheses to try and figure out what will happen in tests or watching. These are not sure things but rather ideas that can be proved or disproved based on real-life proofs. A good theory is clear and can be tested and found wrong if the proof doesn't support it.

Hypothesis Meaning

A hypothesis is a proposed statement that is testable and is given for something that happens or observed.

• It is made using what we already know and have seen, and it's the basis for scientific research.
• A clear guess tells us what we think will happen in an experiment or study.
• It's a testable clue that can be proven true or wrong with real-life facts and checking it out carefully.
• It usually looks like a "if-then" rule, showing the expected cause and effect relationship between what's being studied.

Here are some key characteristics of a hypothesis:

• Testable: An idea (hypothesis) should be made so it can be tested and proven true through doing experiments or watching. It should show a clear connection between things.
• Specific: It needs to be easy and on target, talking about a certain part or connection between things in a study.
• Falsifiable: A good guess should be able to show it's wrong. This means there must be a chance for proof or seeing something that goes against the guess.
• Logical and Rational: It should be based on things we know now or have seen, giving a reasonable reason that fits with what we already know.
• Predictive: A guess often tells what to expect from an experiment or observation. It gives a guide for what someone might see if the guess is right.
• Concise: It should be short and clear, showing the suggested link or explanation simply without extra confusion.
• Grounded in Research: A guess is usually made from before studies, ideas or watching things. It comes from a deep understanding of what is already known in that area.
• Flexible: A guess helps in the research but it needs to change or fix when new information comes up.
• Relevant: It should be related to the question or problem being studied, helping to direct what the research is about.
• Empirical: Hypotheses come from observations and can be tested using methods based on real-world experiences.

Hypotheses can come from different places based on what you're studying and the kind of research. Here are some common sources from which hypotheses may originate:

• Existing Theories: Often, guesses come from well-known science ideas. These ideas may show connections between things or occurrences that scientists can look into more.
• Observation and Experience: Watching something happen or having personal experiences can lead to guesses. We notice odd things or repeat events in everyday life and experiments. This can make us think of guesses called hypotheses.
• Previous Research: Using old studies or discoveries can help come up with new ideas. Scientists might try to expand or question current findings, making guesses that further study old results.
• Literature Review: Looking at books and research in a subject can help make guesses. Noticing missing parts or mismatches in previous studies might make researchers think up guesses to deal with these spots.
• Problem Statement or Research Question: Often, ideas come from questions or problems in the study. Making clear what needs to be looked into can help create ideas that tackle certain parts of the issue.
• Analogies or Comparisons: Making comparisons between similar things or finding connections from related areas can lead to theories. Understanding from other fields could create new guesses in a different situation.
• Hunches and Speculation: Sometimes, scientists might get a gut feeling or make guesses that help create ideas to test. Though these may not have proof at first, they can be a beginning for looking deeper.
• Technology and Innovations: New technology or tools might make guesses by letting us look at things that were hard to study before.
• Personal Interest and Curiosity: People's curiosity and personal interests in a topic can help create guesses. Scientists could make guesses based on their own likes or love for a subject.

Here are some common types of hypotheses:

Simple Hypothesis

Complex hypothesis, directional hypothesis.

• Non-directional Hypothesis

Null Hypothesis (H0)

Alternative hypothesis (h1 or ha), statistical hypothesis, research hypothesis, associative hypothesis, causal hypothesis.

Simple Hypothesis guesses a connection between two things. It says that there is a connection or difference between variables, but it doesn't tell us which way the relationship goes. Example: Studying more can help you do better on tests. Getting more sun makes people have higher amounts of vitamin D.

Complex Hypothesis tells us what will happen when more than two things are connected. It looks at how different things interact and may be linked together. Example: How rich you are, how easy it is to get education and healthcare greatly affects the number of years people live. A new medicine's success relies on the amount used, how old a person is who takes it and their genes.

Directional Hypothesis says how one thing is related to another. For example, it guesses that one thing will help or hurt another thing. Example: Drinking more sweet drinks is linked to a higher body weight score. Too much stress makes people less productive at work.

Non-Directional Hypothesis

Non-Directional Hypothesis are the one that don't say how the relationship between things will be. They just say that there is a connection, without telling which way it goes. Example: Drinking caffeine can affect how well you sleep. People often like different kinds of music based on their gender.

Null hypothesis is a statement that says there's no connection or difference between different things. It implies that any seen impacts are because of luck or random changes in the information. Example: The average test scores of Group A and Group B are not much different. There is no connection between using a certain fertilizer and how much it helps crops grow.

Alternative Hypothesis is different from the null hypothesis and shows that there's a big connection or gap between variables. Scientists want to say no to the null hypothesis and choose the alternative one. Example: Patients on Diet A have much different cholesterol levels than those following Diet B. Exposure to a certain type of light can change how plants grow compared to normal sunlight.

Statistical Hypothesis are used in math testing and include making ideas about what groups or bits of them look like. You aim to get information or test certain things using these top-level, common words only. Example: The average smarts score of kids in a certain school area is 100. The usual time it takes to finish a job using Method A is the same as with Method B.

Research Hypothesis comes from the research question and tells what link is expected between things or factors. It leads the study and chooses where to look more closely. Example: Having more kids go to early learning classes helps them do better in school when they get older. Using specific ways of talking affects how much customers get involved in marketing activities.

Associative Hypothesis guesses that there is a link or connection between things without really saying it caused them. It means that when one thing changes, it is connected to another thing changing. Example: Regular exercise helps to lower the chances of heart disease. Going to school more can help people make more money.

Causal Hypothesis are different from other ideas because they say that one thing causes another. This means there's a cause and effect relationship between variables involved in the situation. They say that when one thing changes, it directly makes another thing change. Example: Playing violent video games makes teens more likely to act aggressively. Less clean air directly impacts breathing health in city populations.

Hypotheses have many important jobs in the process of scientific research. Here are the key functions of hypotheses:

• Guiding Research: Hypotheses give a clear and exact way for research. They act like guides, showing the predicted connections or results that scientists want to study.
• Formulating Research Questions: Research questions often create guesses. They assist in changing big questions into particular, checkable things. They guide what the study should be focused on.
• Setting Clear Objectives: Hypotheses set the goals of a study by saying what connections between variables should be found. They set the targets that scientists try to reach with their studies.
• Testing Predictions: Theories guess what will happen in experiments or observations. By doing tests in a planned way, scientists can check if what they see matches the guesses made by their ideas.
• Providing Structure: Theories give structure to the study process by arranging thoughts and ideas. They aid scientists in thinking about connections between things and plan experiments to match.
• Focusing Investigations: Hypotheses help scientists focus on certain parts of their study question by clearly saying what they expect links or results to be. This focus makes the study work better.
• Facilitating Communication: Theories help scientists talk to each other effectively. Clearly made guesses help scientists to tell others what they plan, how they will do it and the results expected. This explains things well with colleagues in a wide range of audiences.
• Generating Testable Statements: A good guess can be checked, which means it can be looked at carefully or tested by doing experiments. This feature makes sure that guesses add to the real information used in science knowledge.
• Promoting Objectivity: Guesses give a clear reason for study that helps guide the process while reducing personal bias. They motivate scientists to use facts and data as proofs or disprovals for their proposed answers.
• Driving Scientific Progress: Making, trying out and adjusting ideas is a cycle. Even if a guess is proven right or wrong, the information learned helps to grow knowledge in one specific area.

Researchers use hypotheses to put down their thoughts directing how the experiment would take place. Following are the steps that are involved in the scientific method:

• Initiating Investigations: Hypotheses are the beginning of science research. They come from watching, knowing what's already known or asking questions. This makes scientists make certain explanations that need to be checked with tests.
• Formulating Research Questions: Ideas usually come from bigger questions in study. They help scientists make these questions more exact and testable, guiding the study's main point.
• Setting Clear Objectives: Hypotheses set the goals of a study by stating what we think will happen between different things. They set the goals that scientists want to reach by doing their studies.
• Designing Experiments and Studies: Assumptions help plan experiments and watchful studies. They assist scientists in knowing what factors to measure, the techniques they will use and gather data for a proposed reason.
• Testing Predictions: Ideas guess what will happen in experiments or observations. By checking these guesses carefully, scientists can see if the seen results match up with what was predicted in each hypothesis.
• Analysis and Interpretation of Data: Hypotheses give us a way to study and make sense of information. Researchers look at what they found and see if it matches the guesses made in their theories. They decide if the proof backs up or disagrees with these suggested reasons why things are happening as expected.
• Encouraging Objectivity: Hypotheses help make things fair by making sure scientists use facts and information to either agree or disagree with their suggested reasons. They lessen personal preferences by needing proof from experience.
• Iterative Process: People either agree or disagree with guesses, but they still help the ongoing process of science. Findings from testing ideas make us ask new questions, improve those ideas and do more tests. It keeps going on in the work of science to keep learning things.

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Hypothesis is a testable statement serving as an initial explanation for phenomena, based on observations, theories, or existing knowledge . It acts as a guiding light for scientific research, proposing potential relationships between variables that can be empirically tested through experiments and observations.

The hypothesis must be specific, testable, falsifiable, and grounded in prior research or observation, laying out a predictive, if-then scenario that details a cause-and-effect relationship. It originates from various sources including existing theories, observations, previous research, and even personal curiosity, leading to different types, such as simple, complex, directional, non-directional, null, and alternative hypotheses, each serving distinct roles in research methodology .

The hypothesis not only guides the research process by shaping objectives and designing experiments but also facilitates objective analysis and interpretation of data , ultimately driving scientific progress through a cycle of testing, validation, and refinement.

Hypothesis - FAQs

What is a hypothesis.

A guess is a possible explanation or forecast that can be checked by doing research and experiments.

What are Components of a Hypothesis?

The components of a Hypothesis are Independent Variable, Dependent Variable, Relationship between Variables, Directionality etc.

What makes a Good Hypothesis?

Testability, Falsifiability, Clarity and Precision, Relevance are some parameters that makes a Good Hypothesis

Can a Hypothesis be Proven True?

You cannot prove conclusively that most hypotheses are true because it's generally impossible to examine all possible cases for exceptions that would disprove them.

How are Hypotheses Tested?

Hypothesis testing is used to assess the plausibility of a hypothesis by using sample data

Can Hypotheses change during Research?

Yes, you can change or improve your ideas based on new information discovered during the research process.

What is the Role of a Hypothesis in Scientific Research?

Hypotheses are used to support scientific research and bring about advancements in knowledge.

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