- Biology Article
- Study Of Osmosis By Potato Osmometer
Understanding Osmosis Using Potato Osmometer
To study by demonstrating the osmosis process by potato osmometer.
What is Osmosis?
Osmosis is the phenomena in which solvent molecules pass through a semi-permeable membrane from an area of higher concentration to an area of lower concentration. The process continues until the quantity of fluid is balanced or equalized in both regions, the region of higher concentration and the region of lower concentration of the semipermeable membrane. In other words, osmosis is the diffusion or movement of water from a region of higher water potential to a region of lower water potential.
In osmosis, what are solvent and solute?
The fluid that permeates through the semipermeable membrane is called the solvent, whereas the solute is the dissolved particles in the fluid.
What is the solution?
The mixture of solute and solvent form the solution.
List the different types of solutions.
The following are the types of solutions:
- Hypertonic solution – It is a solution with a high solute level. If living cells are placed in a hypertonic solution, because of lower concentration water moves out of the cell causing it to shrink and becomes plasmolyzed.
- Hypotonic solution – It is a solution with low concentration levels of solute. If living cells are placed in this solution, water passes into the cells because of higher water concentration in comparison to the cell causing the cells to swell and turn turgid.
- Isotonic solution – A solution is said to be isotonic if both solutions have an equal concentration of solute. If living cells are placed in an isotonic solution, no change is shown as there is the equal concentration on both the regions hence the cell retains its original shape.
Material Required
- A fresh large-sized potato tuber
- 20% sucrose solution
- Scalpel/blade
- A Bell pin needle that is labelled with a waterproof ink
- Slice the potato tuber into two equal halves with the help of a scalpel or a blade. The outer skin is to be peeled off. Since the tuber shape is irregular, slice the halves into squares
- From the mid-region of the tuber, scoop from the soft parenchyma, so as to form a tiny cavity of a square or a circular shape. At the base, the cavity prepared should have a minimum thickness.
- Fill up half the cavity with the freshly prepared 20% sugar solution. Into the cavity, fix a pin in a way that the mark is in the same line with the layer of the sucrose solution.
- Set up the osmometer in a Petri dish/beaker that is filled with water in a way such that 75% of the potato osmometer is immersed in water
- The set up should remain uninterrupted for close to 1 hour.
- Notice the sugar solution in the osmometer towards the end of the experiment
- Carry out the experiment with the help of water in the cavity and the sucrose solution in the petri dish/beaker.
Observation
After a period of time, within the osmoscope, the sugar solution rises and is seen coloured.
- An increase in the level of sucrose solution is observed in the osmometer. It is because of the entrance of water due to endosmosis from the beaker.
- Also, a water potential gradient is built between the sucrose solution in the external water and the osmometer.
- Though both the liquids are divided by living cells of the potato tuber, they allow the entrance of water into the sugar solution.
- This demonstrates the entrance of water into the sugar solution through the tissues of potato serving as a selectively permeable membrane.
Viva Questions
Q.1. What is plasmolysis?
A.1. It is a process, wherein the protoplasm of the plant cell turns round as a result of contraction when placed in a hypertonic solution due to exosmosis resulting in the decline in the tension of the cell wall.
Q.2. What is the significance of osmosis?
A.2. Osmosis maintains cell turgidity. It causes the transportation of nutrients and discharge of metabolic waste products. It preserves the interior environment of a living entity to maintain an equilibrium between the intracellular fluid levels and water.
Q.3. What is diffusion?
A.3. The movement of molecules from a region of higher concentration to a region of lower concentration. Osmosis is a type of diffusion.
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Study of Osmosis by Potato Osmometer
A study of osmosis can be done using a potato osmometer. Osmosis is a phenomenon in which water moves from high solvent to low solvent concentration. The movement of water occurs between two compartments, separated by a semipermeable membrane .
The cell membrane of living organisms behaves as a semipermeable or selective membrane. The permeability of a selective membrane differs based on the size, charge and mass of different molecules.
Biological membranes are impermeable to large biomolecules and polar molecules like ions. But, non-polar molecules (lipids) and small molecules (oxygen, carbon dioxide etc.) can cross the selective barrier.
Water is the solvent that travels down or up the cell concentration gradient through osmosis. We can study water diffusion by creating two compartments and a semipermeable membrane in between.
The difference in the concentration of solutes or solvents between two compartments is the driving force responsible for water movement. Here, we need to note that only solvents can pass the selective barrier, not solutes.
Thus, the diffusion or distribution of water is related to osmosis . This post describes the meaning, requirements, procedure and results of the potato osmometer experiment.
Content: Study of Osmosis by Potato Osmometer
Potato osmometer, materials required, precautions.
It is a common experiment to demonstrate both endosmosis and exosmosis using a potato. Using a potato Osmoscope, we can study osmosis in a living system.
Here, a potato is used because the porous outer surface of the potato acts as a selective membrane .
- The contents within the cell form one compartment.
- The solution surrounding the cell forms another compartment.
Thus, a selective membrane separates two compartments and allows the process of osmosis .
- High solvent concentration in the cell surrounding.
- Low solvent concentration in the cavity of potato tuber.
Following the rule of osmosis, water in the cell surrounding enters the tuber cavity via the cell membrane.
- High solvent concentration in the cavity of potato tuber.
- Low solvent concentration in the cell surrounding.
Following the rule of osmosis, water in the potato cavity enters the surrounding solution via the cell membrane.
- Peeled off potato
- Concentrated sugar solution
- Petri plate
Video: Study of Osmosis
To perform the potato osmometer experiment, we need to follow the given procedure:
- First, peel off the large-sized potato using a peeler or knife.
- Then cut the upper and lower portions of the peeled potato using a knife. Through this step, we can easily place the potato on the Petri plate.
- Using a knife, make a cavity from the centre of the potato deep into the bottom, leaving some space. Here, the bottom of the potato will function as a selective membrane.
- Then, keep the potato on the Petri plate.
- To study endosmosis , pour water into half of the Petri plate. Next, pour the concentrated sugar solution into half of the cavity created in the potato.
- To study exosmosis , add concentrated sugar solution on the Petri plate and water into the cavity of the potato tuber.
- Then, fix a pin into the potato tuber-A and B to mark the level of sugar solution and water added into the cavity.
- Leave the plate undisturbed for some time until you notice any change.
Observation
- Observe the level of sugar solution in the cavity of potato tuber-A.
- Notice the level of water in the cavity of the potato tuber-B.
Potato Osmosis Experiment Results
- The level of sugar solution in the cavity of potato tuber-A increases . It occurs because the water in the Petri plate will move towards the cell with a high solute or low solvent concentration. This experiment shows endosmosis , as water goes into the cell or potato tuber.
- In contrast, the level of water in the cavity of potato tuber-B decreases . Here, water in the cavity moves toward the solution in the Petri plate due to the high solute concentration in the surrounding. This experiment shows exosmosis as water leaves the cell or potato tuber.
- The cavity should be deep enough by leaving a minimum thickness at the bottom.
- The sugar solution should have a high osmotic concentration.
The water movement from the Petri plate to the potato cavity or vice versa is due to the difference in the solvent or solute concentration between the two compartments.
Related Topics:
- Germination of Plant
- Difference Between Root and Stem
- Nerve Impulse
- Ozone Formation
- Examples of Adsorption in Daily Life
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Science Experiments on the Osmosis of a Potato
Osmosis Experiments With Potatoes for Kids
Osmosis, the process in which solvent molecules move from an area of lower solute concentration to an area of higher solute concentration, can easily be demonstrated with potato experiments. Potatoes are full of both water and starch, and will gain water when immersed in watery solutions. Conversely, they will lose water when in concentrated solutions, such as those containing a great deal of starch. You can use potatoes to set up osmosis experiments for students of all ages and levels.
Potatoes in Saltwater
Cut a potato in two, and immerse one of the halves in a very salty solution of water — one containing a quarter cup of salt in a cup of water. Immerse the other piece in tap water containing no added salt. Leave both in their respective solutions for half an hour, then remove the potato halves from their solutions and observe their differences. The one in the salty solution will have shrunk, indicating that water is diffusing from a less concentrated solution to a more concentrated solution. The one in the tap water solution, in contrast, will actually swell slightly, indicating that it is taking in water.
Salt, Sugar and Pure Water
This experiment helps students to differentiate between different degrees of concentration gradients. Make one salt water solution, one sugar water solution, and for the third solution, simply use tap water. Make three thin potato slices — 1/2 cm thick. Place each potato slice into each of the solutions, and leave the slices in the solutions for a half hour.
Observe that the slice placed in salt is very flexible, while the slice placed in sugar is flexible, but less so. Since potatoes already contain sugar, less water will diffuse out of the potato placed in sugar water. The slice placed in water will be rigid, since it will absorb water.
Potato Lengths in Saline Solutions
Give your students potato "cylinders" that are uniform in length and size: for instance, you could cut them to be 70 mm in length and 7 mm in diameter. Make solutions of saline in three different concentrations, 20 percent, 0.9 percent and 0.1 percent. Have the students measure the lengths and diameters of the potato cylinders before and after soaking them in the saline solutions for half an hour. Then, have them calculate the changes in the lengths and diameters of the cylinders, and plot the saline concentrations versus the changes.
Potato Cube Weights
Cut potatoes into four groups of small, uniform cubes measuring 1/2 cm by 1/2 cm. Make four different solutions of sucrose: 10 percent, 5 percent, 1 percent and 0.01 percent. Weigh each group, on a mass balance, before immersing it in the appropriate sucrose solution for half an hour. After immersion, weigh each group again and have your students calculate the changes in the potato masses. Ask them to comment on why a group gained mass, lost mass or retained the same mass.
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- The Teachers Corner: Science Experiment--Osmosis
About the Author
Tricia Lobo has been writing since 2006. Her biomedical engineering research, "Biocompatible and pH sensitive PLGA encapsulated MnO nanocrystals for molecular and cellular MRI," was accepted in 2010 for publication in the journal "Nanoletters." Lobo earned her Bachelor of Science in biomedical engineering, with distinction, from Yale in 2010.
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Potato Osmosis Experiment
Osmosis is the movement of water from an area of its lower concentration to its higher concentration across a semi-permeable cell membrane. The addition of salt can change the concentration of water and the osmotic gradient. Salt can help us cook. In this osmosis experiment you will see how salt affects potato sticks.
WHAT YOU NEED
What you will do.
Ask an adult to help you cut the potato into even, rectangular sticks.
Do not consume any of the materials used in the experiment!
RECORD YOUR OBSERVATIONS AFTER 6-8 HOURS
CONCLUSIONS
What happened to the potato in the salt water? Why? Which way was the water moving?
What happened to the potato in the regular water? Why? Which way was the water moving?
Copyright @smorescience . All rights reserved. Do not copy, cite, publish, or distribute this content without permission.
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To study and analyze the process of osmosis using potato osmometer. Explore more in detail about Osmosis and Diffusion of water only at BYJU'S.
A study of osmosis can be done using a potato osmometer. This post describes the meaning, requirements, procedure and results of the potato osmometer experiment.
Introduction. Have you ever wondered how plants take up water from the soil? Water uptake in plants is quite complex and involves a process called osmosis. Osmosis makes the water from the soil move into the roots of the plant. But what drives the water from the soil into the plant cells?
Osmosis refers to the movement of water molecules across a membrane trying to achieve equilibrium. Because there are no salts in distilled water, there is a higher concentration of water molecules in the distilled water compared to inside the potato. Therefore water moves INTO the potato.
The paper considers the process of osmosis using a potato osmometer, an apparatus that clearly shows this particular process. In the experiment, we will see how water is moved from high to low water potential across a semipermeable membrane and how different solutions affect this process.
Potato osmometer showing osmosis. • Place the osmometer in a beaker/petridish filled with water in such a way that 2/3rd of the potato osmometer is dipped in water. • Leave the set up undisturbed for about an hour. • Observe the level of sugar solution in the osmometer at the end of experiment.
Osmosis, the process in which solvent molecules move from an area of lower solute concentration to an area of higher solute concentration, can easily be demonstrated with potato experiments. Potatoes are full of both water and starch, and will gain water when immersed in watery solutions.
Potato Osmosis Experiment. Osmosis is the movement of water from an area of its lower concentration to its higher concentration across a semi-permeable cell membrane. The addition of salt can change the concentration of water and the osmotic gradient. Salt can help us cook.
This experiment shows the effect of osmosis on plant tissue. The cylinders will decrease or increase in mass if they lose or gain water by osmosis.
Explanation of the potato osmometer experiment. This animation shows the changes in liquid content within the first two potato halves.The graphics below explain the non-appearance of liquid in the other two cases: In the case of the 3rd potato half, there is no solute to start the process off so the cavity remains dry.