Population Growth: Experimental Models Using Duckweed (Lemna spp.)
Learn how to study population growth and resource competition of duckweeds (Lemna spp.) in the lab. This resource provides three experiments with protocols, data collection, and analysis.
A Population Study
Part A. Duckweed Population Growth 1. Use a marker to label two Petri dishes with your name, date, and 1 or 2, respectively. ... During the remainder of the experiment, replace any lost water volume with distilled or spring water. 11. Count the number of fronds present each week for the next six weeks. Try to count the number of fronds on the ...
PDF Experiment # Lemna minor (Duckweed) Population Growth
Conduct an experiment on population growth using scientific techniques including observation, data collection, and analysis ... The growth of a population of duckweed can be determined by simply counting the number of thalli that appear over a period of time. 4. Divide the class into groups of three to five, depending on the amount of materials ...
PDF POPULATION GROWTH IN DUCKWEED
Exponential growth means that populations numbers grow at an increasing rate. Mathematically, we could write: Populations at some point in time = starting population size raised to some power. where is Nt is the population at time t, No is the starting population, and r is some power, like 2.
Skimming the surface: duckweed as a model system in ecology and evolution
growth. Duckweeds are ranked among the fastest growing and most productive higher plants (e.g., Ziegler et al., 2015), often completely blanketing the surface of their water body (Fig. 1). This rapid growth is part of what makes duckweed useful for industrial applications, but is also advantageous for fundamental research in ecology and
PDF Experiment # Lemna minor (Duckweed) Population Growth
Experiment # Lemna minor (Duckweed) Population Growth Introduction - Students will grow duckweed (Lemna minor) over a two to three week period to observe what happens to a population of organisms when allowed to grow without predation or competition. Objectives-Understand some of the factors that affect population growth, like method of reproduction (asexual or
Using Duckweed in the Lab
Learn how to grow and investigate duckweed, a tiny but robust plant that can double every three days. Find out how duckweed can be used for bioremediation and eutrophication studies.
Automated imaging of duckweed growth and development
As a proof‐of‐concept experiment, we grew duckweed on semi‐solid media with and without sucrose and monitored its effect on their growth over 3 days. ... Time‐lapse imaging of duckweed growth. We conducted a 67 h growth time‐lapse on duckweed accessions L. minor 9253. In the 12‐well plate, half of the wells were supplemented with 0. ...
American Journal of Botany
Each experiment lasted 15 days, allowing for three to four generations of both duckweeds and aphids. We quantified population growth of the aphids by counting their abundances five times over 15 days. We quantified duckweed performance in two ways, both of which represent multigenerational fitness.
PDF MODELING THE GROWTH OF DUCKWEED POPULATIONS
Logistic Growth. In order to model this, Verhulst modified the exponential growth equation to make the population size proportional to both the original population and a new term K. dN/dt = r x N ...
Duckweed growth model for large-scale applications: Optimizing
Duckweed has emerged as a potential feedstock for the environmentally sustainable and economically viable production of biofuels and protein. The aim of this study was to: (1) enhance an existing intrinsic duckweed growth model and use it to develop a general regression model that enables users to easily predict annual duckweed yield for large scale applications; and (2) determine the optimal ...
Creation of culture media for efficient duckweeds micropropagation
For this, experiments Nos. 1-6, 9, 10, 12, 13 were carried out independently of each other. ... Biomass growth of duckweed cultivated on the maximal yield medium (a) for W. arrhiza ... the equations based on the value of the total water-soluble protein concentration derived from the resulting duckweed population, ...
The Importance and Potential of Duckweeds as a Model and ...
In follow-up experiments they found they could increase duckweed starch content from 9.5% to 40% through 11 days of growth on clean water, and that a hydraulic residence time (HRT) of 6 days achieved their treatment standards and optimized the Landoltia punctata starch yield above maize and wheat to 13.9 t/ha year. For their size, length of ...
PDF Polyploidy impacts population growth and competition with diploids
33 polyploidy at the population level remain largely untested even though establishment is 34 an intrinsically population-level process. 35 • We conducted population-level experiments where investment in current and future 36 growth was tracked in four lineage pairs of diploids and synthetic neopolyploids of the
Researchers reveal growth characteristics of duckweed in
Citation: Researchers reveal growth characteristics of duckweed in photoautotrophic, heterotrophic and mixotrophic conditions (2020, October 15) retrieved 5 September 2024 from https://phys.org ...
Influence of Light Intensity and Spectrum on Duckweed Growth and
2.1. Indoor Vertical Farm. Two duckweed species, Lemna minor L. (clone 9441; Germany) and Wolffiella hyalina Delile Monod (clone 9525; India), were chosen for the experiments due to their fast growth rates and high protein contents [].The plant material was obtained from the Duckweed Stock Collection of the Department of Plant Physiology, University of Jena, Germany.
The effect of pH on the population growth of three species of duckweed
Rates of population growth are similar for all species. In decreasing order they are: Wolffia, Lemna, Spirodela. However, in biomass units Lemna grew more than six and Spirodela seventeen times ...
Light intensity drives different growth strategies in two duckweed
In duckweed growth inhibition tests, 100 µmol photons m −2 s −1 of continuous light is recommended (e.g., ISO 20079, 2005 cited in Ziegler et al., 2015), which corresponds to the low light intensity applied in our experiment. Our clones of both species performed better under the higher light level and a 16:8 photoperiod, but multiplicated ...
PDF TIEE
Created Date: 5/20/1999 1:56:30 PM
Duckweeds: Current Biology
When duckweeds face growth-limiting conditions in the environment (e.g., over-wintering), some species develop starch-rich vegetative progeny called turions that sink to the bottom of the aquatic environment and remain dormant until the conditions are favorable again. ... therefore making genetic experiments difficult, duckweed fell out of ...
Duckweed Lab Report (1)
However, the growth rate of Duckweed in a smaller space, 0.106, was significantly different than the growth rate in a larger space, 0.138. Population growth rates were affected by the consumption of space, a limiting resource. Duckweed populations did not reach the carrying capacity in the duration of the experiment.
Population Growth
Students are introduced to the ecology of population growth by growing duckweed and learning about invasive species.
Duckweeds: their utilization, metabolites and cultivation
Light intensity and duckweed growth rate show a direct relationship, unless the light intensity is too high. The lowest growth rate was at a low intensity of 6 μmol m-2 s −1, and the growth rate increased with increasing light intensity to 1000 μmol m-2 s −1 . Duckweeds accumulate antioxidants to prevent damage when exposed to excessive ...
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Learn how to study population growth and resource competition of duckweeds (Lemna spp.) in the lab. This resource provides three experiments with protocols, data collection, and analysis.
Part A. Duckweed Population Growth 1. Use a marker to label two Petri dishes with your name, date, and 1 or 2, respectively. ... During the remainder of the experiment, replace any lost water volume with distilled or spring water. 11. Count the number of fronds present each week for the next six weeks. Try to count the number of fronds on the ...
Conduct an experiment on population growth using scientific techniques including observation, data collection, and analysis ... The growth of a population of duckweed can be determined by simply counting the number of thalli that appear over a period of time. 4. Divide the class into groups of three to five, depending on the amount of materials ...
Exponential growth means that populations numbers grow at an increasing rate. Mathematically, we could write: Populations at some point in time = starting population size raised to some power. where is Nt is the population at time t, No is the starting population, and r is some power, like 2.
growth. Duckweeds are ranked among the fastest growing and most productive higher plants (e.g., Ziegler et al., 2015), often completely blanketing the surface of their water body (Fig. 1). This rapid growth is part of what makes duckweed useful for industrial applications, but is also advantageous for fundamental research in ecology and
Experiment # Lemna minor (Duckweed) Population Growth Introduction - Students will grow duckweed (Lemna minor) over a two to three week period to observe what happens to a population of organisms when allowed to grow without predation or competition. Objectives-Understand some of the factors that affect population growth, like method of reproduction (asexual or
Learn how to grow and investigate duckweed, a tiny but robust plant that can double every three days. Find out how duckweed can be used for bioremediation and eutrophication studies.
As a proof‐of‐concept experiment, we grew duckweed on semi‐solid media with and without sucrose and monitored its effect on their growth over 3 days. ... Time‐lapse imaging of duckweed growth. We conducted a 67 h growth time‐lapse on duckweed accessions L. minor 9253. In the 12‐well plate, half of the wells were supplemented with 0. ...
Each experiment lasted 15 days, allowing for three to four generations of both duckweeds and aphids. We quantified population growth of the aphids by counting their abundances five times over 15 days. We quantified duckweed performance in two ways, both of which represent multigenerational fitness.
Logistic Growth. In order to model this, Verhulst modified the exponential growth equation to make the population size proportional to both the original population and a new term K. dN/dt = r x N ...
Duckweed has emerged as a potential feedstock for the environmentally sustainable and economically viable production of biofuels and protein. The aim of this study was to: (1) enhance an existing intrinsic duckweed growth model and use it to develop a general regression model that enables users to easily predict annual duckweed yield for large scale applications; and (2) determine the optimal ...
For this, experiments Nos. 1-6, 9, 10, 12, 13 were carried out independently of each other. ... Biomass growth of duckweed cultivated on the maximal yield medium (a) for W. arrhiza ... the equations based on the value of the total water-soluble protein concentration derived from the resulting duckweed population, ...
In follow-up experiments they found they could increase duckweed starch content from 9.5% to 40% through 11 days of growth on clean water, and that a hydraulic residence time (HRT) of 6 days achieved their treatment standards and optimized the Landoltia punctata starch yield above maize and wheat to 13.9 t/ha year. For their size, length of ...
33 polyploidy at the population level remain largely untested even though establishment is 34 an intrinsically population-level process. 35 • We conducted population-level experiments where investment in current and future 36 growth was tracked in four lineage pairs of diploids and synthetic neopolyploids of the
Citation: Researchers reveal growth characteristics of duckweed in photoautotrophic, heterotrophic and mixotrophic conditions (2020, October 15) retrieved 5 September 2024 from https://phys.org ...
2.1. Indoor Vertical Farm. Two duckweed species, Lemna minor L. (clone 9441; Germany) and Wolffiella hyalina Delile Monod (clone 9525; India), were chosen for the experiments due to their fast growth rates and high protein contents [].The plant material was obtained from the Duckweed Stock Collection of the Department of Plant Physiology, University of Jena, Germany.
Rates of population growth are similar for all species. In decreasing order they are: Wolffia, Lemna, Spirodela. However, in biomass units Lemna grew more than six and Spirodela seventeen times ...
In duckweed growth inhibition tests, 100 µmol photons m −2 s −1 of continuous light is recommended (e.g., ISO 20079, 2005 cited in Ziegler et al., 2015), which corresponds to the low light intensity applied in our experiment. Our clones of both species performed better under the higher light level and a 16:8 photoperiod, but multiplicated ...
Created Date: 5/20/1999 1:56:30 PM
When duckweeds face growth-limiting conditions in the environment (e.g., over-wintering), some species develop starch-rich vegetative progeny called turions that sink to the bottom of the aquatic environment and remain dormant until the conditions are favorable again. ... therefore making genetic experiments difficult, duckweed fell out of ...
However, the growth rate of Duckweed in a smaller space, 0.106, was significantly different than the growth rate in a larger space, 0.138. Population growth rates were affected by the consumption of space, a limiting resource. Duckweed populations did not reach the carrying capacity in the duration of the experiment.
Students are introduced to the ecology of population growth by growing duckweed and learning about invasive species.
Light intensity and duckweed growth rate show a direct relationship, unless the light intensity is too high. The lowest growth rate was at a low intensity of 6 μmol m-2 s −1, and the growth rate increased with increasing light intensity to 1000 μmol m-2 s −1 . Duckweeds accumulate antioxidants to prevent damage when exposed to excessive ...