what is a null hypothesis in psychology

Research Hypothesis In Psychology: Types, & Examples

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BSc (Hons) Psychology, MRes, PhD, University of Manchester

Saul McLeod, PhD., is a qualified psychology teacher with over 18 years of experience in further and higher education. He has been published in peer-reviewed journals, including the Journal of Clinical Psychology.

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A research hypothesis, in its plural form “hypotheses,” is a specific, testable prediction about the anticipated results of a study, established at its outset. It is a key component of the scientific method .

Hypotheses connect theory to data and guide the research process towards expanding scientific understanding

Some key points about hypotheses:

• A hypothesis expresses an expected pattern or relationship. It connects the variables under investigation.
• It is stated in clear, precise terms before any data collection or analysis occurs. This makes the hypothesis testable.
• A hypothesis must be falsifiable. It should be possible, even if unlikely in practice, to collect data that disconfirms rather than supports the hypothesis.
• Hypotheses guide research. Scientists design studies to explicitly evaluate hypotheses about how nature works.
• For a hypothesis to be valid, it must be testable against empirical evidence. The evidence can then confirm or disprove the testable predictions.
• Hypotheses are informed by background knowledge and observation, but go beyond what is already known to propose an explanation of how or why something occurs.

Predictions typically arise from a thorough knowledge of the research literature, curiosity about real-world problems or implications, and integrating this to advance theory. They build on existing literature while providing new insight.

Types of Research Hypotheses

Alternative hypothesis.

The research hypothesis is often called the alternative or experimental hypothesis in experimental research.

It typically suggests a potential relationship between two key variables: the independent variable, which the researcher manipulates, and the dependent variable, which is measured based on those changes.

The alternative hypothesis states a relationship exists between the two variables being studied (one variable affects the other).

A hypothesis is a testable statement or prediction about the relationship between two or more variables. It is a key component of the scientific method. Some key points about hypotheses:

• Important hypotheses lead to predictions that can be tested empirically. The evidence can then confirm or disprove the testable predictions.

In summary, a hypothesis is a precise, testable statement of what researchers expect to happen in a study and why. Hypotheses connect theory to data and guide the research process towards expanding scientific understanding.

An experimental hypothesis predicts what change(s) will occur in the dependent variable when the independent variable is manipulated.

It states that the results are not due to chance and are significant in supporting the theory being investigated.

The alternative hypothesis can be directional, indicating a specific direction of the effect, or non-directional, suggesting a difference without specifying its nature. It’s what researchers aim to support or demonstrate through their study.

Null Hypothesis

The null hypothesis states no relationship exists between the two variables being studied (one variable does not affect the other). There will be no changes in the dependent variable due to manipulating the independent variable.

It states results are due to chance and are not significant in supporting the idea being investigated.

The null hypothesis, positing no effect or relationship, is a foundational contrast to the research hypothesis in scientific inquiry. It establishes a baseline for statistical testing, promoting objectivity by initiating research from a neutral stance.

Many statistical methods are tailored to test the null hypothesis, determining the likelihood of observed results if no true effect exists.

This dual-hypothesis approach provides clarity, ensuring that research intentions are explicit, and fosters consistency across scientific studies, enhancing the standardization and interpretability of research outcomes.

Nondirectional Hypothesis

A non-directional hypothesis, also known as a two-tailed hypothesis, predicts that there is a difference or relationship between two variables but does not specify the direction of this relationship.

It merely indicates that a change or effect will occur without predicting which group will have higher or lower values.

For example, “There is a difference in performance between Group A and Group B” is a non-directional hypothesis.

Directional Hypothesis

A directional (one-tailed) hypothesis predicts the nature of the effect of the independent variable on the dependent variable. It predicts in which direction the change will take place. (i.e., greater, smaller, less, more)

It specifies whether one variable is greater, lesser, or different from another, rather than just indicating that there’s a difference without specifying its nature.

For example, “Exercise increases weight loss” is a directional hypothesis.

Falsifiability

The Falsification Principle, proposed by Karl Popper , is a way of demarcating science from non-science. It suggests that for a theory or hypothesis to be considered scientific, it must be testable and irrefutable.

Falsifiability emphasizes that scientific claims shouldn’t just be confirmable but should also have the potential to be proven wrong.

It means that there should exist some potential evidence or experiment that could prove the proposition false.

However many confirming instances exist for a theory, it only takes one counter observation to falsify it. For example, the hypothesis that “all swans are white,” can be falsified by observing a black swan.

For Popper, science should attempt to disprove a theory rather than attempt to continually provide evidence to support a research hypothesis.

Can a Hypothesis be Proven?

Hypotheses make probabilistic predictions. They state the expected outcome if a particular relationship exists. However, a study result supporting a hypothesis does not definitively prove it is true.

All studies have limitations. There may be unknown confounding factors or issues that limit the certainty of conclusions. Additional studies may yield different results.

In science, hypotheses can realistically only be supported with some degree of confidence, not proven. The process of science is to incrementally accumulate evidence for and against hypothesized relationships in an ongoing pursuit of better models and explanations that best fit the empirical data. But hypotheses remain open to revision and rejection if that is where the evidence leads.

• Disproving a hypothesis is definitive. Solid disconfirmatory evidence will falsify a hypothesis and require altering or discarding it based on the evidence.
• However, confirming evidence is always open to revision. Other explanations may account for the same results, and additional or contradictory evidence may emerge over time.

We can never 100% prove the alternative hypothesis. Instead, we see if we can disprove, or reject the null hypothesis.

If we reject the null hypothesis, this doesn’t mean that our alternative hypothesis is correct but does support the alternative/experimental hypothesis.

Upon analysis of the results, an alternative hypothesis can be rejected or supported, but it can never be proven to be correct. We must avoid any reference to results proving a theory as this implies 100% certainty, and there is always a chance that evidence may exist which could refute a theory.

How to Write a Hypothesis

• Identify variables . The researcher manipulates the independent variable and the dependent variable is the measured outcome.
• Operationalized the variables being investigated . Operationalization of a hypothesis refers to the process of making the variables physically measurable or testable, e.g. if you are about to study aggression, you might count the number of punches given by participants.
• Decide on a direction for your prediction . If there is evidence in the literature to support a specific effect of the independent variable on the dependent variable, write a directional (one-tailed) hypothesis. If there are limited or ambiguous findings in the literature regarding the effect of the independent variable on the dependent variable, write a non-directional (two-tailed) hypothesis.
• Make it Testable : Ensure your hypothesis can be tested through experimentation or observation. It should be possible to prove it false (principle of falsifiability).
• Clear & concise language . A strong hypothesis is concise (typically one to two sentences long), and formulated using clear and straightforward language, ensuring it’s easily understood and testable.

Consider a hypothesis many teachers might subscribe to: students work better on Monday morning than on Friday afternoon (IV=Day, DV= Standard of work).

Now, if we decide to study this by giving the same group of students a lesson on a Monday morning and a Friday afternoon and then measuring their immediate recall of the material covered in each session, we would end up with the following:

• The alternative hypothesis states that students will recall significantly more information on a Monday morning than on a Friday afternoon.
• The null hypothesis states that there will be no significant difference in the amount recalled on a Monday morning compared to a Friday afternoon. Any difference will be due to chance or confounding factors.

More Examples

• Memory : Participants exposed to classical music during study sessions will recall more items from a list than those who studied in silence.
• Social Psychology : Individuals who frequently engage in social media use will report higher levels of perceived social isolation compared to those who use it infrequently.
• Developmental Psychology : Children who engage in regular imaginative play have better problem-solving skills than those who don’t.
• Clinical Psychology : Cognitive-behavioral therapy will be more effective in reducing symptoms of anxiety over a 6-month period compared to traditional talk therapy.
• Cognitive Psychology : Individuals who multitask between various electronic devices will have shorter attention spans on focused tasks than those who single-task.
• Health Psychology : Patients who practice mindfulness meditation will experience lower levels of chronic pain compared to those who don’t meditate.
• Organizational Psychology : Employees in open-plan offices will report higher levels of stress than those in private offices.
• Behavioral Psychology : Rats rewarded with food after pressing a lever will press it more frequently than rats who receive no reward.

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Chapter 13: Inferential Statistics

Understanding Null Hypothesis Testing

Learning Objectives

• Explain the purpose of null hypothesis testing, including the role of sampling error.
• Describe the basic logic of null hypothesis testing.
• Describe the role of relationship strength and sample size in determining statistical significance and make reasonable judgments about statistical significance based on these two factors.

The Purpose of Null Hypothesis Testing

As we have seen, psychological research typically involves measuring one or more variables for a sample and computing descriptive statistics for that sample. In general, however, the researcher’s goal is not to draw conclusions about that sample but to draw conclusions about the population that the sample was selected from. Thus researchers must use sample statistics to draw conclusions about the corresponding values in the population. These corresponding values in the population are called  parameters . Imagine, for example, that a researcher measures the number of depressive symptoms exhibited by each of 50 clinically depressed adults and computes the mean number of symptoms. The researcher probably wants to use this sample statistic (the mean number of symptoms for the sample) to draw conclusions about the corresponding population parameter (the mean number of symptoms for clinically depressed adults).

Unfortunately, sample statistics are not perfect estimates of their corresponding population parameters. This is because there is a certain amount of random variability in any statistic from sample to sample. The mean number of depressive symptoms might be 8.73 in one sample of clinically depressed adults, 6.45 in a second sample, and 9.44 in a third—even though these samples are selected randomly from the same population. Similarly, the correlation (Pearson’s  r ) between two variables might be +.24 in one sample, −.04 in a second sample, and +.15 in a third—again, even though these samples are selected randomly from the same population. This random variability in a statistic from sample to sample is called  sampling error . (Note that the term error  here refers to random variability and does not imply that anyone has made a mistake. No one “commits a sampling error.”)

One implication of this is that when there is a statistical relationship in a sample, it is not always clear that there is a statistical relationship in the population. A small difference between two group means in a sample might indicate that there is a small difference between the two group means in the population. But it could also be that there is no difference between the means in the population and that the difference in the sample is just a matter of sampling error. Similarly, a Pearson’s  r  value of −.29 in a sample might mean that there is a negative relationship in the population. But it could also be that there is no relationship in the population and that the relationship in the sample is just a matter of sampling error.

In fact, any statistical relationship in a sample can be interpreted in two ways:

• There is a relationship in the population, and the relationship in the sample reflects this.
• There is no relationship in the population, and the relationship in the sample reflects only sampling error.

The purpose of null hypothesis testing is simply to help researchers decide between these two interpretations.

The Logic of Null Hypothesis Testing

Null hypothesis testing  is a formal approach to deciding between two interpretations of a statistical relationship in a sample. One interpretation is called the   null hypothesis  (often symbolized  H 0  and read as “H-naught”). This is the idea that there is no relationship in the population and that the relationship in the sample reflects only sampling error. Informally, the null hypothesis is that the sample relationship “occurred by chance.” The other interpretation is called the  alternative hypothesis  (often symbolized as  H 1 ). This is the idea that there is a relationship in the population and that the relationship in the sample reflects this relationship in the population.

Again, every statistical relationship in a sample can be interpreted in either of these two ways: It might have occurred by chance, or it might reflect a relationship in the population. So researchers need a way to decide between them. Although there are many specific null hypothesis testing techniques, they are all based on the same general logic. The steps are as follows:

• Assume for the moment that the null hypothesis is true. There is no relationship between the variables in the population.
• Determine how likely the sample relationship would be if the null hypothesis were true.
• If the sample relationship would be extremely unlikely, then reject the null hypothesis  in favour of the alternative hypothesis. If it would not be extremely unlikely, then  retain the null hypothesis .

Following this logic, we can begin to understand why Mehl and his colleagues concluded that there is no difference in talkativeness between women and men in the population. In essence, they asked the following question: “If there were no difference in the population, how likely is it that we would find a small difference of  d  = 0.06 in our sample?” Their answer to this question was that this sample relationship would be fairly likely if the null hypothesis were true. Therefore, they retained the null hypothesis—concluding that there is no evidence of a sex difference in the population. We can also see why Kanner and his colleagues concluded that there is a correlation between hassles and symptoms in the population. They asked, “If the null hypothesis were true, how likely is it that we would find a strong correlation of +.60 in our sample?” Their answer to this question was that this sample relationship would be fairly unlikely if the null hypothesis were true. Therefore, they rejected the null hypothesis in favour of the alternative hypothesis—concluding that there is a positive correlation between these variables in the population.

A crucial step in null hypothesis testing is finding the likelihood of the sample result if the null hypothesis were true. This probability is called the  p value . A low  p  value means that the sample result would be unlikely if the null hypothesis were true and leads to the rejection of the null hypothesis. A high  p  value means that the sample result would be likely if the null hypothesis were true and leads to the retention of the null hypothesis. But how low must the  p  value be before the sample result is considered unlikely enough to reject the null hypothesis? In null hypothesis testing, this criterion is called  α (alpha)  and is almost always set to .05. If there is less than a 5% chance of a result as extreme as the sample result if the null hypothesis were true, then the null hypothesis is rejected. When this happens, the result is said to be  statistically significant . If there is greater than a 5% chance of a result as extreme as the sample result when the null hypothesis is true, then the null hypothesis is retained. This does not necessarily mean that the researcher accepts the null hypothesis as true—only that there is not currently enough evidence to conclude that it is true. Researchers often use the expression “fail to reject the null hypothesis” rather than “retain the null hypothesis,” but they never use the expression “accept the null hypothesis.”

The Misunderstood  p  Value

The  p  value is one of the most misunderstood quantities in psychological research (Cohen, 1994) [1] . Even professional researchers misinterpret it, and it is not unusual for such misinterpretations to appear in statistics textbooks!

The most common misinterpretation is that the  p  value is the probability that the null hypothesis is true—that the sample result occurred by chance. For example, a misguided researcher might say that because the  p  value is .02, there is only a 2% chance that the result is due to chance and a 98% chance that it reflects a real relationship in the population. But this is incorrect . The  p  value is really the probability of a result at least as extreme as the sample result  if  the null hypothesis  were  true. So a  p  value of .02 means that if the null hypothesis were true, a sample result this extreme would occur only 2% of the time.

You can avoid this misunderstanding by remembering that the  p  value is not the probability that any particular  hypothesis  is true or false. Instead, it is the probability of obtaining the  sample result  if the null hypothesis were true.

Role of Sample Size and Relationship Strength

Recall that null hypothesis testing involves answering the question, “If the null hypothesis were true, what is the probability of a sample result as extreme as this one?” In other words, “What is the  p  value?” It can be helpful to see that the answer to this question depends on just two considerations: the strength of the relationship and the size of the sample. Specifically, the stronger the sample relationship and the larger the sample, the less likely the result would be if the null hypothesis were true. That is, the lower the  p  value. This should make sense. Imagine a study in which a sample of 500 women is compared with a sample of 500 men in terms of some psychological characteristic, and Cohen’s  d  is a strong 0.50. If there were really no sex difference in the population, then a result this strong based on such a large sample should seem highly unlikely. Now imagine a similar study in which a sample of three women is compared with a sample of three men, and Cohen’s  d  is a weak 0.10. If there were no sex difference in the population, then a relationship this weak based on such a small sample should seem likely. And this is precisely why the null hypothesis would be rejected in the first example and retained in the second.

Of course, sometimes the result can be weak and the sample large, or the result can be strong and the sample small. In these cases, the two considerations trade off against each other so that a weak result can be statistically significant if the sample is large enough and a strong relationship can be statistically significant even if the sample is small. Table 13.1 shows roughly how relationship strength and sample size combine to determine whether a sample result is statistically significant. The columns of the table represent the three levels of relationship strength: weak, medium, and strong. The rows represent four sample sizes that can be considered small, medium, large, and extra large in the context of psychological research. Thus each cell in the table represents a combination of relationship strength and sample size. If a cell contains the word  Yes , then this combination would be statistically significant for both Cohen’s  d  and Pearson’s  r . If it contains the word  No , then it would not be statistically significant for either. There is one cell where the decision for  d  and  r  would be different and another where it might be different depending on some additional considerations, which are discussed in Section 13.2 “Some Basic Null Hypothesis Tests”

Although Table 13.1 provides only a rough guideline, it shows very clearly that weak relationships based on medium or small samples are never statistically significant and that strong relationships based on medium or larger samples are always statistically significant. If you keep this lesson in mind, you will often know whether a result is statistically significant based on the descriptive statistics alone. It is extremely useful to be able to develop this kind of intuitive judgment. One reason is that it allows you to develop expectations about how your formal null hypothesis tests are going to come out, which in turn allows you to detect problems in your analyses. For example, if your sample relationship is strong and your sample is medium, then you would expect to reject the null hypothesis. If for some reason your formal null hypothesis test indicates otherwise, then you need to double-check your computations and interpretations. A second reason is that the ability to make this kind of intuitive judgment is an indication that you understand the basic logic of this approach in addition to being able to do the computations.

Statistical Significance Versus Practical Significance

Table 13.1 illustrates another extremely important point. A statistically significant result is not necessarily a strong one. Even a very weak result can be statistically significant if it is based on a large enough sample. This is closely related to Janet Shibley Hyde’s argument about sex differences (Hyde, 2007) [2] . The differences between women and men in mathematical problem solving and leadership ability are statistically significant. But the word  significant  can cause people to interpret these differences as strong and important—perhaps even important enough to influence the college courses they take or even who they vote for. As we have seen, however, these statistically significant differences are actually quite weak—perhaps even “trivial.”

This is why it is important to distinguish between the  statistical  significance of a result and the  practical  significance of that result.  Practical significance refers to the importance or usefulness of the result in some real-world context. Many sex differences are statistically significant—and may even be interesting for purely scientific reasons—but they are not practically significant. In clinical practice, this same concept is often referred to as “clinical significance.” For example, a study on a new treatment for social phobia might show that it produces a statistically significant positive effect. Yet this effect still might not be strong enough to justify the time, effort, and other costs of putting it into practice—especially if easier and cheaper treatments that work almost as well already exist. Although statistically significant, this result would be said to lack practical or clinical significance.

Key Takeaways

• Null hypothesis testing is a formal approach to deciding whether a statistical relationship in a sample reflects a real relationship in the population or is just due to chance.
• The logic of null hypothesis testing involves assuming that the null hypothesis is true, finding how likely the sample result would be if this assumption were correct, and then making a decision. If the sample result would be unlikely if the null hypothesis were true, then it is rejected in favour of the alternative hypothesis. If it would not be unlikely, then the null hypothesis is retained.
• The probability of obtaining the sample result if the null hypothesis were true (the  p  value) is based on two considerations: relationship strength and sample size. Reasonable judgments about whether a sample relationship is statistically significant can often be made by quickly considering these two factors.
• Statistical significance is not the same as relationship strength or importance. Even weak relationships can be statistically significant if the sample size is large enough. It is important to consider relationship strength and the practical significance of a result in addition to its statistical significance.
• Discussion: Imagine a study showing that people who eat more broccoli tend to be happier. Explain for someone who knows nothing about statistics why the researchers would conduct a null hypothesis test.
• The correlation between two variables is  r  = −.78 based on a sample size of 137.
• The mean score on a psychological characteristic for women is 25 ( SD  = 5) and the mean score for men is 24 ( SD  = 5). There were 12 women and 10 men in this study.
• In a memory experiment, the mean number of items recalled by the 40 participants in Condition A was 0.50 standard deviations greater than the mean number recalled by the 40 participants in Condition B.
• In another memory experiment, the mean scores for participants in Condition A and Condition B came out exactly the same!
• A student finds a correlation of  r  = .04 between the number of units the students in his research methods class are taking and the students’ level of stress.

Long Descriptions

“Null Hypothesis” long description: A comic depicting a man and a woman talking in the foreground. In the background is a child working at a desk. The man says to the woman, “I can’t believe schools are still teaching kids about the null hypothesis. I remember reading a big study that conclusively disproved it years ago.” [Return to “Null Hypothesis”]

“Conditional Risk” long description: A comic depicting two hikers beside a tree during a thunderstorm. A bolt of lightning goes “crack” in the dark sky as thunder booms. One of the hikers says, “Whoa! We should get inside!” The other hiker says, “It’s okay! Lightning only kills about 45 Americans a year, so the chances of dying are only one in 7,000,000. Let’s go on!” The comic’s caption says, “The annual death rate among people who know that statistic is one in six.” [Return to “Conditional Risk”]

Media Attributions

• Null Hypothesis by XKCD  CC BY-NC (Attribution NonCommercial)
• Conditional Risk by XKCD  CC BY-NC (Attribution NonCommercial)
• Cohen, J. (1994). The world is round: p < .05. American Psychologist, 49 , 997–1003. ↵
• Hyde, J. S. (2007). New directions in the study of gender similarities and differences. Current Directions in Psychological Science, 16 , 259–263. ↵

Values in a population that correspond to variables measured in a study.

The random variability in a statistic from sample to sample.

A formal approach to deciding between two interpretations of a statistical relationship in a sample.

The idea that there is no relationship in the population and that the relationship in the sample reflects only sampling error.

The idea that there is a relationship in the population and that the relationship in the sample reflects this relationship in the population.

When the relationship found in the sample would be extremely unlikely, the idea that the relationship occurred “by chance” is rejected.

When the relationship found in the sample is likely to have occurred by chance, the null hypothesis is not rejected.

The probability that, if the null hypothesis were true, the result found in the sample would occur.

How low the p value must be before the sample result is considered unlikely in null hypothesis testing.

When there is less than a 5% chance of a result as extreme as the sample result occurring and the null hypothesis is rejected.

Research Methods in Psychology - 2nd Canadian Edition Copyright © 2015 by Paul C. Price, Rajiv Jhangiani, & I-Chant A. Chiang is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License , except where otherwise noted.

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• Null and Alternative Hypotheses | Definitions & Examples

Null & Alternative Hypotheses | Definitions, Templates & Examples

Published on May 6, 2022 by Shaun Turney . Revised on June 22, 2023.

The null and alternative hypotheses are two competing claims that researchers weigh evidence for and against using a statistical test :

• Null hypothesis ( H 0 ): There’s no effect in the population .
• Alternative hypothesis ( H a or H 1 ) : There’s an effect in the population.

Table of contents

Answering your research question with hypotheses, what is a null hypothesis, what is an alternative hypothesis, similarities and differences between null and alternative hypotheses, how to write null and alternative hypotheses, other interesting articles, frequently asked questions.

The null and alternative hypotheses offer competing answers to your research question . When the research question asks “Does the independent variable affect the dependent variable?”:

• The null hypothesis ( H 0 ) answers “No, there’s no effect in the population.”
• The alternative hypothesis ( H a ) answers “Yes, there is an effect in the population.”

The null and alternative are always claims about the population. That’s because the goal of hypothesis testing is to make inferences about a population based on a sample . Often, we infer whether there’s an effect in the population by looking at differences between groups or relationships between variables in the sample. It’s critical for your research to write strong hypotheses .

You can use a statistical test to decide whether the evidence favors the null or alternative hypothesis. Each type of statistical test comes with a specific way of phrasing the null and alternative hypothesis. However, the hypotheses can also be phrased in a general way that applies to any test.

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The null hypothesis is the claim that there’s no effect in the population.

If the sample provides enough evidence against the claim that there’s no effect in the population ( p ≤ α), then we can reject the null hypothesis . Otherwise, we fail to reject the null hypothesis.

Although “fail to reject” may sound awkward, it’s the only wording that statisticians accept . Be careful not to say you “prove” or “accept” the null hypothesis.

Null hypotheses often include phrases such as “no effect,” “no difference,” or “no relationship.” When written in mathematical terms, they always include an equality (usually =, but sometimes ≥ or ≤).

You can never know with complete certainty whether there is an effect in the population. Some percentage of the time, your inference about the population will be incorrect. When you incorrectly reject the null hypothesis, it’s called a type I error . When you incorrectly fail to reject it, it’s a type II error.

Examples of null hypotheses

The table below gives examples of research questions and null hypotheses. There’s always more than one way to answer a research question, but these null hypotheses can help you get started.

*Note that some researchers prefer to always write the null hypothesis in terms of “no effect” and “=”. It would be fine to say that daily meditation has no effect on the incidence of depression and p 1 = p 2 .

The alternative hypothesis ( H a ) is the other answer to your research question . It claims that there’s an effect in the population.

Often, your alternative hypothesis is the same as your research hypothesis. In other words, it’s the claim that you expect or hope will be true.

The alternative hypothesis is the complement to the null hypothesis. Null and alternative hypotheses are exhaustive, meaning that together they cover every possible outcome. They are also mutually exclusive, meaning that only one can be true at a time.

Alternative hypotheses often include phrases such as “an effect,” “a difference,” or “a relationship.” When alternative hypotheses are written in mathematical terms, they always include an inequality (usually ≠, but sometimes < or >). As with null hypotheses, there are many acceptable ways to phrase an alternative hypothesis.

Examples of alternative hypotheses

The table below gives examples of research questions and alternative hypotheses to help you get started with formulating your own.

Null and alternative hypotheses are similar in some ways:

• They’re both answers to the research question.
• They both make claims about the population.
• They’re both evaluated by statistical tests.

However, there are important differences between the two types of hypotheses, summarized in the following table.

To help you write your hypotheses, you can use the template sentences below. If you know which statistical test you’re going to use, you can use the test-specific template sentences. Otherwise, you can use the general template sentences.

General template sentences

The only thing you need to know to use these general template sentences are your dependent and independent variables. To write your research question, null hypothesis, and alternative hypothesis, fill in the following sentences with your variables:

Does independent variable affect dependent variable ?

• Null hypothesis ( H 0 ): Independent variable does not affect dependent variable.
• Alternative hypothesis ( H a ): Independent variable affects dependent variable.

Test-specific template sentences

Once you know the statistical test you’ll be using, you can write your hypotheses in a more precise and mathematical way specific to the test you chose. The table below provides template sentences for common statistical tests.

Note: The template sentences above assume that you’re performing one-tailed tests . One-tailed tests are appropriate for most studies.

If you want to know more about statistics , methodology , or research bias , make sure to check out some of our other articles with explanations and examples.

• Normal distribution
• Descriptive statistics
• Measures of central tendency
• Correlation coefficient

Methodology

• Cluster sampling
• Stratified sampling
• Types of interviews
• Cohort study
• Thematic analysis

Research bias

• Implicit bias
• Cognitive bias
• Survivorship bias
• Availability heuristic
• Nonresponse bias
• Regression to the mean

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

Null and alternative hypotheses are used in statistical hypothesis testing . The null hypothesis of a test always predicts no effect or no relationship between variables, while the alternative hypothesis states your research prediction of an effect or relationship.

The null hypothesis is often abbreviated as H 0 . When the null hypothesis is written using mathematical symbols, it always includes an equality symbol (usually =, but sometimes ≥ or ≤).

The alternative hypothesis is often abbreviated as H a or H 1 . When the alternative hypothesis is written using mathematical symbols, it always includes an inequality symbol (usually ≠, but sometimes < or >).

A research hypothesis is your proposed answer to your research question. The research hypothesis usually includes an explanation (“ x affects y because …”).

A statistical hypothesis, on the other hand, is a mathematical statement about a population parameter. Statistical hypotheses always come in pairs: the null and alternative hypotheses . In a well-designed study , the statistical hypotheses correspond logically to the research hypothesis.

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Introduction to Hypothesis Testing (Psychology)

Contents Toggle Main Menu 1 What is a Hypothesis test? 2 The Null and Alternative Hypotheses 3 The Structure of a Hypothesis Test 3.1 Summary of Steps for a Hypothesis Test 4 P -Values 5 Parametric and Non-Parametric Hypothesis Tests 6 One and two tailed tests 7 Type I and Type II Errors 8 See Also 9 Worksheets

What is a Hypothesis test?

A statistical hypothesis is an unproven statement which can be tested. A hypothesis test is used to test whether this statement is true.

The Null and Alternative Hypotheses

• The null hypothesis $H_0$, is where you assume that the observations are statistically independent i.e. no difference in the populations you are testing. If the null hypothesis is true, it suggests that any changes witnessed in an experiment are because of random chance and not because of changes made to variables in the experiment. For example, serotonin levels have no effect on ability to cope with stress. See also Null and alternative hypotheses .
• The alternative hypothesis $H_1$, is a theory that the observations are related (not independent) in some way. We only adopt the alternative hypothesis if we have rejected the null hypothesis. For example, serotonin levels affect a person's ability to cope with stress. You do not necessarily have to specify in what way they are related but can do (see one and two tailed tests for more information).

The Structure of a Hypothesis Test

• The first step of a hypothesis test is to state the null hypothesis $H_0$ and the alternative hypothesis $H_1$ . The null hypothesis is the statement or claim being made (which we are trying to disprove) and the alternative hypothesis is the hypothesis that we are trying to prove and which is accepted if we have sufficient evidence to reject the null hypothesis.

For example, consider a person in court who is charged with murder. The jury needs to decide whether the person in innocent (the null hypothesis) or guilty (the alternative hypothesis). As usual, we assume the person is innocent unless the jury can provide sufficient evidence that the person is guilty. Similarly, we assume that $H_0$ is true unless we can provide sufficient evidence that it is false and that $H_1$ is true, in which case we reject $H_0$ and accept $H_1$.

To decide if we have sufficient evidence against the null hypothesis to reject it (in favour of the alternative hypothesis), we must first decide upon a significance level . The significance level is the probability of rejecting the null hypothesis when it the null hypothesis is true and is denoted by $\alpha$. The $5\%$ significance level is a common choice for statistical test.

The next step is to collect data and calculate the test statistic and associated $p$-value using the data. Assuming that the null hypothesis is true, the $p$-value is the probability of obtaining a sample statistic equal to or more extreme than the observed test statistic.

Next we must compare the $p$-value with the chosen significance level. If $p \lt \alpha$ then we reject $H_0$ and accept $H_1$. The lower $p$, the more evidence we have against $H_0$ and so the more confidence we can have that $H_0$ is false. If $p \geq \alpha$ then we do not have sufficient evidence to reject the $H_0$ and so must accept it.

Alternatively, we can compare our test statistic with the appropriate critical value for the chosen significance level. We can look up critical values in distribution tables (see worked examples below). If our test statistic is:

• positive and greater than the critical value, then we have sufficient evidence to reject the null hypothesis and accept the alternative hypothesis.
• positive and lower than or equal to the critical value, we must accept the null hypothesis.
• negative and lower than the critical value, then we have sufficient evidence to reject the null hypothesis and accept the alternative hypothesis.
• negative and greater than or equal to the critical value, we must accept the null hypothesis.

For either method:

Significant difference found: Reject the null hypothesis No significant difference found: Accept the null hypothesis

Finally, we must interpret our results and come to a conclusion. Returning to the example of the person in court, if the result of our hypothesis test indicated that we should accept $H_1$ and reject $H_0$, our conclusion would be that the jury should declare the person guilty of murder.

Summary of Steps for a Hypothesis Test

• Specify the null and the alternative hypothesis
• Decide upon the significance level.
• Comparing the $p$-value to the significance level $\alpha$, or
• Comparing the test statistic to the critical value.
• Interpret your results and draw a conclusion

P -Values"> P -Values

The $p$ -value is the probability of the test statistic (e.g. t -value or Chi-Square value) occurring given the null hypothesis is true. Since it is a probability, the $p$-value is a number between $0$ and $1$.

• Typically $p \leq 0.05$ shows that there is strong evidence for $H_1$ so we can accept it and reject $H_0$. Any $p$-value less than $0.05$ is significant and $p$-values less than $0.01$ are very significant .
• Typically $ p > 0.05$ shows that there is poor evidence for $H_1$ so we reject it and accept $H_0$.
• The smaller the $p$-value the more evidence there is supporting the hypothesis.
• The rule for accepting and rejecting the hypothesis is:

\begin{align} \text {Significant difference found} &= \textbf{Reject}\text{ the null hypothesis}\\ \text {No Significant difference found} &= \textbf{Accept}\text{ the null hypothesis}\\ \end{align}

• Note : The significance level is not always $0.05$. It can differ depending on the application and is often subjective (different people will have different opinions on what values are appropriate). For example, if lives are at stake then the $p$-value must be very small for safety reasons.
• See $P$-values for further detail on this topic.

Parametric and Non-Parametric Hypothesis Tests

There are parametric and non-parametric hypothesis tests.

• A parametric hypothesis assumes that the data follows a Normal probability distribution (with equal variances if we are working with more than one set of data) . A parametric hypothesis test is a statement about the parameters of this distribution (typically the mean). This can be seen in more detail in the Parametric Hypotheses Tests section .
• A non-parametric test assumes that the data does not follow any distribution and usually bases its calculations on the median . Note that although we assume the data does not follow a particular distribution it may do anyway. This can be seen in more detail in the Non-Parametric Hypotheses Tests section .

One and two tailed tests

Whether a test is One-tailed or Two-tailed is appropriate depends upon the alternative hypothesis $H_1$.

• One-tailed tests are used when the alternative hypothesis states that the parameter of interest is either bigger or smaller than the value stated in the null hypothesis. For example, the null hypothesis might state that the average weight of chocolate bars produced by a chocolate factory in Slough is 35g (as is printed on the wrapper), while the alternative hypothesis might state that the average weight of the chocolate bars is in fact lower than 35g.
• Two-tailed tests are used when the hypothesis states that the parameter of interest differs from the null hypothesis but does not specify in which direction. In the above example, a Two-tailed alternative hypothesis would be that the average weight of the chocolate bars is not equal to 35g.

Type I and Type II Errors

• A Type I error is made if we reject the null hypothesis when it is true (so should have been accepted). Returning to the example of the person in court, a Type I error would be made if the jury declared the person guilty when they are in fact innocent. The probability of making a Type I error is equal to the significance level $\alpha$.
• A Type II error is made if we accept the null hypothesis when it is false i.e. we should have rejected the null hypothesis and accepted the alternative hypothesis. This would occur if the jury declared the person innocent when they are in fact guilty.

For more information about the topics covered here see hypothesis testing .

• Introduction to hypothesis testing
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Null Hypothesis Definition and Examples

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In a scientific experiment, the null hypothesis is the proposition that there is no effect or no relationship between phenomena or populations. If the null hypothesis is true, any observed difference in phenomena or populations would be due to sampling error (random chance) or experimental error. The null hypothesis is useful because it can be tested and found to be false, which then implies that there is a relationship between the observed data. It may be easier to think of it as a nullifiable hypothesis or one that the researcher seeks to nullify. The null hypothesis is also known as the H 0, or no-difference hypothesis.

The alternate hypothesis, H A or H 1 , proposes that observations are influenced by a non-random factor. In an experiment, the alternate hypothesis suggests that the experimental or independent variable has an effect on the dependent variable .

How to State a Null Hypothesis

There are two ways to state a null hypothesis. One is to state it as a declarative sentence, and the other is to present it as a mathematical statement.

For example, say a researcher suspects that exercise is correlated to weight loss, assuming diet remains unchanged. The average length of time to achieve a certain amount of weight loss is six weeks when a person works out five times a week. The researcher wants to test whether weight loss takes longer to occur if the number of workouts is reduced to three times a week.

The first step to writing the null hypothesis is to find the (alternate) hypothesis. In a word problem like this, you're looking for what you expect to be the outcome of the experiment. In this case, the hypothesis is "I expect weight loss to take longer than six weeks."

This can be written mathematically as: H 1 : μ > 6

In this example, μ is the average.

Now, the null hypothesis is what you expect if this hypothesis does not happen. In this case, if weight loss isn't achieved in greater than six weeks, then it must occur at a time equal to or less than six weeks. This can be written mathematically as:

H 0 : μ ≤ 6

The other way to state the null hypothesis is to make no assumption about the outcome of the experiment. In this case, the null hypothesis is simply that the treatment or change will have no effect on the outcome of the experiment. For this example, it would be that reducing the number of workouts would not affect the time needed to achieve weight loss:

H 0 : μ = 6

Null Hypothesis Examples

"Hyperactivity is unrelated to eating sugar " is an example of a null hypothesis. If the hypothesis is tested and found to be false, using statistics, then a connection between hyperactivity and sugar ingestion may be indicated. A significance test is the most common statistical test used to establish confidence in a null hypothesis.

Another example of a null hypothesis is "Plant growth rate is unaffected by the presence of cadmium in the soil ." A researcher could test the hypothesis by measuring the growth rate of plants grown in a medium lacking cadmium, compared with the growth rate of plants grown in mediums containing different amounts of cadmium. Disproving the null hypothesis would set the groundwork for further research into the effects of different concentrations of the element in soil.

Why Test a Null Hypothesis?

You may be wondering why you would want to test a hypothesis just to find it false. Why not just test an alternate hypothesis and find it true? The short answer is that it is part of the scientific method. In science, propositions are not explicitly "proven." Rather, science uses math to determine the probability that a statement is true or false. It turns out it's much easier to disprove a hypothesis than to positively prove one. Also, while the null hypothesis may be simply stated, there's a good chance the alternate hypothesis is incorrect.

For example, if your null hypothesis is that plant growth is unaffected by duration of sunlight, you could state the alternate hypothesis in several different ways. Some of these statements might be incorrect. You could say plants are harmed by more than 12 hours of sunlight or that plants need at least three hours of sunlight, etc. There are clear exceptions to those alternate hypotheses, so if you test the wrong plants, you could reach the wrong conclusion. The null hypothesis is a general statement that can be used to develop an alternate hypothesis, which may or may not be correct.

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Inferential Statistics

Learning Objectives

• Explain the purpose of null hypothesis testing, including the role of sampling error.
• Describe the basic logic of null hypothesis testing.
• Describe the role of relationship strength and sample size in determining statistical significance and make reasonable judgments about statistical significance based on these two factors.

 The Purpose of Null Hypothesis Testing

As we have seen, psychological research typically involves measuring one or more variables in a sample and computing descriptive summary data (e.g., means, correlation coefficients) for those variables. These descriptive data for the sample are called statistics .  In general, however, the researcher’s goal is not to draw conclusions about that sample but to draw conclusions about the population that the sample was selected from. Thus researchers must use sample statistics to draw conclusions about the corresponding values in the population. These corresponding values in the population are called parameters . Imagine, for example, that a researcher measures the number of depressive symptoms exhibited by each of 50 adults with clinical depression and computes the mean number of symptoms. The researcher probably wants to use this sample statistic (the mean number of symptoms for the sample) to draw conclusions about the corresponding population parameter (the mean number of symptoms for adults with clinical depression).

Unfortunately, sample statistics are not perfect estimates of their corresponding population parameters. This is because there is a certain amount of random variability in any statistic from sample to sample. The mean number of depressive symptoms might be 8.73 in one sample of adults with clinical depression, 6.45 in a second sample, and 9.44 in a third—even though these samples are selected randomly from the same population. Similarly, the correlation (Pearson’s  r ) between two variables might be +.24 in one sample, −.04 in a second sample, and +.15 in a third—again, even though these samples are selected randomly from the same population. This random variability in a statistic from sample to sample is called  sampling error . (Note that the term error  here refers to random variability and does not imply that anyone has made a mistake. No one “commits a sampling error.”)

One implication of this is that when there is a statistical relationship in a sample, it is not always clear that there is a statistical relationship in the population. A small difference between two group means in a sample might indicate that there is a small difference between the two group means in the population. But it could also be that there is no difference between the means in the population and that the difference in the sample is just a matter of sampling error. Similarly, a Pearson’s  r  value of −.29 in a sample might mean that there is a negative relationship in the population. But it could also be that there is no relationship in the population and that the relationship in the sample is just a matter of sampling error.

In fact, any statistical relationship in a sample can be interpreted in two ways:

• There is a relationship in the population, and the relationship in the sample reflects this.
• There is no relationship in the population, and the relationship in the sample reflects only sampling error.

The purpose of null hypothesis testing is simply to help researchers decide between these two interpretations.

The Logic of Null Hypothesis Testing

Null hypothesis testing (often called null hypothesis significance testing or NHST) is a formal approach to deciding between two interpretations of a statistical relationship in a sample. One interpretation is called the   null hypothesis  (often symbolized  H 0 and read as “H-zero”). This is the idea that there is no relationship in the population and that the relationship in the sample reflects only sampling error. Informally, the null hypothesis is that the sample relationship “occurred by chance.” The other interpretation is called the alternative hypothesis  (often symbolized as  H 1 ). This is the idea that there is a relationship in the population and that the relationship in the sample reflects this relationship in the population.

Again, every statistical relationship in a sample can be interpreted in either of these two ways: It might have occurred by chance, or it might reflect a relationship in the population. So researchers need a way to decide between them. Although there are many specific null hypothesis testing techniques, they are all based on the same general logic. The steps are as follows:

• Assume for the moment that the null hypothesis is true. There is no relationship between the variables in the population.
• Determine how likely the sample relationship would be if the null hypothesis were true.
• If the sample relationship would be extremely unlikely, then reject the null hypothesis  in favor of the alternative hypothesis. If it would not be extremely unlikely, then  retain the null hypothesis .

Following this logic, we can begin to understand why Mehl and his colleagues concluded that there is no difference in talkativeness between women and men in the population. In essence, they asked the following question: “If there were no difference in the population, how likely is it that we would find a small difference of  d  = 0.06 in our sample?” Their answer to this question was that this sample relationship would be fairly likely if the null hypothesis were true. Therefore, they retained the null hypothesis—concluding that there is no evidence of a sex difference in the population. We can also see why Kanner and his colleagues concluded that there is a correlation between hassles and symptoms in the population. They asked, “If the null hypothesis were true, how likely is it that we would find a strong correlation of +.60 in our sample?” Their answer to this question was that this sample relationship would be fairly unlikely if the null hypothesis were true. Therefore, they rejected the null hypothesis in favor of the alternative hypothesis—concluding that there is a positive correlation between these variables in the population.

A crucial step in null hypothesis testing is finding the probability of the sample result or a more extreme result if the null hypothesis were true (Lakens, 2017). [1] This probability is called the p value . A low  p value means that the sample or more extreme result would be unlikely if the null hypothesis were true and leads to the rejection of the null hypothesis. A p value that is not low means that the sample or more extreme result would be likely if the null hypothesis were true and leads to the retention of the null hypothesis. But how low must the p value criterion be before the sample result is considered unlikely enough to reject the null hypothesis? In null hypothesis testing, this criterion is called α (alpha) and is almost always set to .05. If there is a 5% chance or less of a result at least as extreme as the sample result if the null hypothesis were true, then the null hypothesis is rejected. When this happens, the result is said to be statistically significant . If there is greater than a 5% chance of a result as extreme as the sample result when the null hypothesis is true, then the null hypothesis is retained. This does not necessarily mean that the researcher accepts the null hypothesis as true—only that there is not currently enough evidence to reject it. Researchers often use the expression “fail to reject the null hypothesis” rather than “retain the null hypothesis,” but they never use the expression “accept the null hypothesis.”

The Misunderstood  p  Value

The  p  value is one of the most misunderstood quantities in psychological research (Cohen, 1994) [2] . Even professional researchers misinterpret it, and it is not unusual for such misinterpretations to appear in statistics textbooks!

The most common misinterpretation is that the  p  value is the probability that the null hypothesis is true—that the sample result occurred by chance. For example, a misguided researcher might say that because the  p  value is .02, there is only a 2% chance that the result is due to chance and a 98% chance that it reflects a real relationship in the population. But this is incorrect . The  p  value is really the probability of a result at least as extreme as the sample result  if  the null hypothesis  were  true. So a  p  value of .02 means that if the null hypothesis were true, a sample result this extreme would occur only 2% of the time.

You can avoid this misunderstanding by remembering that the  p  value is not the probability that any particular  hypothesis  is true or false. Instead, it is the probability of obtaining the  sample result  if the null hypothesis were true.

Role of Sample Size and Relationship Strength

Recall that null hypothesis testing involves answering the question, “If the null hypothesis were true, what is the probability of a sample result as extreme as this one?” In other words, “What is the  p  value?” It can be helpful to see that the answer to this question depends on just two considerations: the strength of the relationship and the size of the sample. Specifically, the stronger the sample relationship and the larger the sample, the less likely the result would be if the null hypothesis were true. That is, the lower the  p  value. This should make sense. Imagine a study in which a sample of 500 women is compared with a sample of 500 men in terms of some psychological characteristic, and Cohen’s  d  is a strong 0.50. If there were really no sex difference in the population, then a result this strong based on such a large sample should seem highly unlikely. Now imagine a similar study in which a sample of three women is compared with a sample of three men, and Cohen’s  d  is a weak 0.10. If there were no sex difference in the population, then a relationship this weak based on such a small sample should seem likely. And this is precisely why the null hypothesis would be rejected in the first example and retained in the second.

Of course, sometimes the result can be weak and the sample large, or the result can be strong and the sample small. In these cases, the two considerations trade off against each other so that a weak result can be statistically significant if the sample is large enough and a strong relationship can be statistically significant even if the sample is small. Table 13.1 shows roughly how relationship strength and sample size combine to determine whether a sample result is statistically significant. The columns of the table represent the three levels of relationship strength: weak, medium, and strong. The rows represent four sample sizes that can be considered small, medium, large, and extra large in the context of psychological research. Thus each cell in the table represents a combination of relationship strength and sample size. If a cell contains the word  Yes , then this combination would be statistically significant for both Cohen’s  d  and Pearson’s  r . If it contains the word  No , then it would not be statistically significant for either. There is one cell where the decision for  d  and  r  would be different and another where it might be different depending on some additional considerations, which are discussed in Section 13.2 “Some Basic Null Hypothesis Tests”

Although Table 13.1 provides only a rough guideline, it shows very clearly that weak relationships based on medium or small samples are never statistically significant and that strong relationships based on medium or larger samples are always statistically significant. If you keep this lesson in mind, you will often know whether a result is statistically significant based on the descriptive statistics alone. It is extremely useful to be able to develop this kind of intuitive judgment. One reason is that it allows you to develop expectations about how your formal null hypothesis tests are going to come out, which in turn allows you to detect problems in your analyses. For example, if your sample relationship is strong and your sample is medium, then you would expect to reject the null hypothesis. If for some reason your formal null hypothesis test indicates otherwise, then you need to double-check your computations and interpretations. A second reason is that the ability to make this kind of intuitive judgment is an indication that you understand the basic logic of this approach in addition to being able to do the computations.

Statistical Significance Versus Practical Significance

Table 13.1 illustrates another extremely important point. A statistically significant result is not necessarily a strong one. Even a very weak result can be statistically significant if it is based on a large enough sample. This is closely related to Janet Shibley Hyde’s argument about sex differences (Hyde, 2007) [3] . The differences between women and men in mathematical problem solving and leadership ability are statistically significant. But the word  significant  can cause people to interpret these differences as strong and important—perhaps even important enough to influence the college courses they take or even who they vote for. As we have seen, however, these statistically significant differences are actually quite weak—perhaps even “trivial.”

This is why it is important to distinguish between the  statistical  significance of a result and the  practical  significance of that result.  Practical significance refers to the importance or usefulness of the result in some real-world context. Many sex differences are statistically significant—and may even be interesting for purely scientific reasons—but they are not practically significant. In clinical practice, this same concept is often referred to as “clinical significance.” For example, a study on a new treatment for social phobia might show that it produces a statistically significant positive effect. Yet this effect still might not be strong enough to justify the time, effort, and other costs of putting it into practice—especially if easier and cheaper treatments that work almost as well already exist. Although statistically significant, this result would be said to lack practical or clinical significance.

Image Description

“Null Hypothesis” long description:  A comic depicting a man and a woman talking in the foreground. In the background is a child working at a desk. The man says to the woman, “I can’t believe schools are still teaching kids about the null hypothesis. I remember reading a big study that conclusively disproved it  years  ago.”  [Return to “Null Hypothesis”]

“Conditional Risk” long description:  A comic depicting two hikers beside a tree during a thunderstorm. A bolt of lightning goes “crack” in the dark sky as thunder booms. One of the hikers says, “Whoa! We should get inside!” The other hiker says, “It’s okay! Lightning only kills about 45 Americans a year, so the chances of dying are only one in 7,000,000. Let’s go on!” The comic’s caption says, “The annual death rate among people who know that statistic is one in six.”  [Return to “Conditional Risk”]

Media Attributions

• Null Hypothesis  by XKCD  CC BY-NC (Attribution NonCommercial)
• Conditional Risk  by XKCD  CC BY-NC (Attribution NonCommercial)
• Lakens, D. (2017, December 25). About p -values: Understanding common misconceptions. [Blog post] Retrieved from https://correlaid.org/en/blog/understand-p-values/ ↵
• Cohen, J. (1994). The world is round: p < .05. American Psychologist, 49 , 997–1003. ↵
• Hyde, J. S. (2007). New directions in the study of gender similarities and differences. Current Directions in Psychological Science, 16 , 259–263. ↵

Descriptive data that involves measuring one or more variables in a sample and computing descriptive summary data (e.g., means, correlation coefficients) for those variables.

Corresponding values in the population.

The random variability in a statistic from sample to sample.

A formal approach to deciding between two interpretations of a statistical relationship in a sample.

The idea that there is no relationship in the population and that the relationship in the sample reflects only sampling error (often symbolized H0 and read as “H-zero”).

An alternative to the null hypothesis (often symbolized as H1), this hypothesis proposes that there is a relationship in the population and that the relationship in the sample reflects this relationship in the population.

A decision made by researchers using null hypothesis testing which occurs when the sample relationship would be extremely unlikely.

A decision made by researchers in null hypothesis testing which occurs when the sample relationship would not be extremely unlikely.

The probability of obtaining the sample result or a more extreme result if the null hypothesis were true.

The criterion that shows how low a p-value should be before the sample result is considered unlikely enough to reject the null hypothesis (Usually set to .05).

An effect that is unlikely due to random chance and therefore likely represents a real effect in the population.

Refers to the importance or usefulness of the result in some real-world context.

Research Methods in Psychology Copyright © 2019 by Rajiv S. Jhangiani, I-Chant A. Chiang, Carrie Cuttler, & Dana C. Leighton is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License , except where otherwise noted.

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How to Write a Great Hypothesis

Hypothesis Definition, Format, Examples, and Tips

Verywell / Alex Dos Diaz

• The Scientific Method

Hypothesis Format

Falsifiability of a hypothesis.

• Operationalization

Hypothesis Types

Hypotheses examples.

• Collecting Data

A hypothesis is a tentative statement about the relationship between two or more variables. It is a specific, testable prediction about what you expect to happen in a study. It is a preliminary answer to your question that helps guide the research process.

Consider a study designed to examine the relationship between sleep deprivation and test performance. The hypothesis might be: "This study is designed to assess the hypothesis that sleep-deprived people will perform worse on a test than individuals who are not sleep-deprived."

At a Glance

A hypothesis is crucial to scientific research because it offers a clear direction for what the researchers are looking to find. This allows them to design experiments to test their predictions and add to our scientific knowledge about the world. This article explores how a hypothesis is used in psychology research, how to write a good hypothesis, and the different types of hypotheses you might use.

The Hypothesis in the Scientific Method

In the scientific method , whether it involves research in psychology, biology, or some other area, a hypothesis represents what the researchers think will happen in an experiment. The scientific method involves the following steps:

• Forming a question
• Performing background research
• Creating a hypothesis
• Designing an experiment
• Collecting data
• Analyzing the results
• Drawing conclusions
• Communicating the results

The hypothesis is a prediction, but it involves more than a guess. Most of the time, the hypothesis begins with a question which is then explored through background research. At this point, researchers then begin to develop a testable hypothesis.

Unless you are creating an exploratory study, your hypothesis should always explain what you  expect  to happen.

In a study exploring the effects of a particular drug, the hypothesis might be that researchers expect the drug to have some type of effect on the symptoms of a specific illness. In psychology, the hypothesis might focus on how a certain aspect of the environment might influence a particular behavior.

Remember, a hypothesis does not have to be correct. While the hypothesis predicts what the researchers expect to see, the goal of the research is to determine whether this guess is right or wrong. When conducting an experiment, researchers might explore numerous factors to determine which ones might contribute to the ultimate outcome.

In many cases, researchers may find that the results of an experiment  do not  support the original hypothesis. When writing up these results, the researchers might suggest other options that should be explored in future studies.

In many cases, researchers might draw a hypothesis from a specific theory or build on previous research. For example, prior research has shown that stress can impact the immune system. So a researcher might hypothesize: "People with high-stress levels will be more likely to contract a common cold after being exposed to the virus than people who have low-stress levels."

In other instances, researchers might look at commonly held beliefs or folk wisdom. "Birds of a feather flock together" is one example of folk adage that a psychologist might try to investigate. The researcher might pose a specific hypothesis that "People tend to select romantic partners who are similar to them in interests and educational level."

Elements of a Good Hypothesis

So how do you write a good hypothesis? When trying to come up with a hypothesis for your research or experiments, ask yourself the following questions:

• Is your hypothesis based on your research on a topic?
• Can your hypothesis be tested?
• Does your hypothesis include independent and dependent variables?

Before you come up with a specific hypothesis, spend some time doing background research. Once you have completed a literature review, start thinking about potential questions you still have. Pay attention to the discussion section in the  journal articles you read . Many authors will suggest questions that still need to be explored.

How to Formulate a Good Hypothesis

To form a hypothesis, you should take these steps:

• Collect as many observations about a topic or problem as you can.
• Evaluate these observations and look for possible causes of the problem.
• Create a list of possible explanations that you might want to explore.
• After you have developed some possible hypotheses, think of ways that you could confirm or disprove each hypothesis through experimentation. This is known as falsifiability.

In the scientific method ,  falsifiability is an important part of any valid hypothesis. In order to test a claim scientifically, it must be possible that the claim could be proven false.

Students sometimes confuse the idea of falsifiability with the idea that it means that something is false, which is not the case. What falsifiability means is that  if  something was false, then it is possible to demonstrate that it is false.

One of the hallmarks of pseudoscience is that it makes claims that cannot be refuted or proven false.

The Importance of Operational Definitions

A variable is a factor or element that can be changed and manipulated in ways that are observable and measurable. However, the researcher must also define how the variable will be manipulated and measured in the study.

Operational definitions are specific definitions for all relevant factors in a study. This process helps make vague or ambiguous concepts detailed and measurable.

For example, a researcher might operationally define the variable " test anxiety " as the results of a self-report measure of anxiety experienced during an exam. A "study habits" variable might be defined by the amount of studying that actually occurs as measured by time.

These precise descriptions are important because many things can be measured in various ways. Clearly defining these variables and how they are measured helps ensure that other researchers can replicate your results.

Replicability

One of the basic principles of any type of scientific research is that the results must be replicable.

Replication means repeating an experiment in the same way to produce the same results. By clearly detailing the specifics of how the variables were measured and manipulated, other researchers can better understand the results and repeat the study if needed.

Some variables are more difficult than others to define. For example, how would you operationally define a variable such as aggression ? For obvious ethical reasons, researchers cannot create a situation in which a person behaves aggressively toward others.

To measure this variable, the researcher must devise a measurement that assesses aggressive behavior without harming others. The researcher might utilize a simulated task to measure aggressiveness in this situation.

Hypothesis Checklist

• Does your hypothesis focus on something that you can actually test?
• Does your hypothesis include both an independent and dependent variable?
• Can you manipulate the variables?
• Can your hypothesis be tested without violating ethical standards?

The hypothesis you use will depend on what you are investigating and hoping to find. Some of the main types of hypotheses that you might use include:

• Simple hypothesis : This type of hypothesis suggests there is a relationship between one independent variable and one dependent variable.
• Complex hypothesis : This type suggests a relationship between three or more variables, such as two independent and dependent variables.
• Null hypothesis : This hypothesis suggests no relationship exists between two or more variables.
• Alternative hypothesis : This hypothesis states the opposite of the null hypothesis.
• Statistical hypothesis : This hypothesis uses statistical analysis to evaluate a representative population sample and then generalizes the findings to the larger group.
• Logical hypothesis : This hypothesis assumes a relationship between variables without collecting data or evidence.

A hypothesis often follows a basic format of "If {this happens} then {this will happen}." One way to structure your hypothesis is to describe what will happen to the  dependent variable  if you change the  independent variable .

The basic format might be: "If {these changes are made to a certain independent variable}, then we will observe {a change in a specific dependent variable}."

A few examples of simple hypotheses:

• "Students who eat breakfast will perform better on a math exam than students who do not eat breakfast."
• "Students who experience test anxiety before an English exam will get lower scores than students who do not experience test anxiety."​
• "Motorists who talk on the phone while driving will be more likely to make errors on a driving course than those who do not talk on the phone."
• "Children who receive a new reading intervention will have higher reading scores than students who do not receive the intervention."

Examples of a complex hypothesis include:

• "People with high-sugar diets and sedentary activity levels are more likely to develop depression."
• "Younger people who are regularly exposed to green, outdoor areas have better subjective well-being than older adults who have limited exposure to green spaces."

Examples of a null hypothesis include:

• "There is no difference in anxiety levels between people who take St. John's wort supplements and those who do not."
• "There is no difference in scores on a memory recall task between children and adults."
• "There is no difference in aggression levels between children who play first-person shooter games and those who do not."

Examples of an alternative hypothesis:

• "People who take St. John's wort supplements will have less anxiety than those who do not."
• "Adults will perform better on a memory task than children."
• "Children who play first-person shooter games will show higher levels of aggression than children who do not." 

Collecting Data on Your Hypothesis

Once a researcher has formed a testable hypothesis, the next step is to select a research design and start collecting data. The research method depends largely on exactly what they are studying. There are two basic types of research methods: descriptive research and experimental research.

Descriptive Research Methods

Descriptive research such as  case studies ,  naturalistic observations , and surveys are often used when  conducting an experiment is difficult or impossible. These methods are best used to describe different aspects of a behavior or psychological phenomenon.

Once a researcher has collected data using descriptive methods, a  correlational study  can examine how the variables are related. This research method might be used to investigate a hypothesis that is difficult to test experimentally.

Experimental Research Methods

Experimental methods  are used to demonstrate causal relationships between variables. In an experiment, the researcher systematically manipulates a variable of interest (known as the independent variable) and measures the effect on another variable (known as the dependent variable).

Unlike correlational studies, which can only be used to determine if there is a relationship between two variables, experimental methods can be used to determine the actual nature of the relationship—whether changes in one variable actually  cause  another to change.

The hypothesis is a critical part of any scientific exploration. It represents what researchers expect to find in a study or experiment. In situations where the hypothesis is unsupported by the research, the research still has value. Such research helps us better understand how different aspects of the natural world relate to one another. It also helps us develop new hypotheses that can then be tested in the future.

Thompson WH, Skau S. On the scope of scientific hypotheses .  R Soc Open Sci . 2023;10(8):230607. doi:10.1098/rsos.230607

Taran S, Adhikari NKJ, Fan E. Falsifiability in medicine: what clinicians can learn from Karl Popper [published correction appears in Intensive Care Med. 2021 Jun 17;:].  Intensive Care Med . 2021;47(9):1054-1056. doi:10.1007/s00134-021-06432-z

Eyler AA. Research Methods for Public Health . 1st ed. Springer Publishing Company; 2020. doi:10.1891/9780826182067.0004

Nosek BA, Errington TM. What is replication ?  PLoS Biol . 2020;18(3):e3000691. doi:10.1371/journal.pbio.3000691

Aggarwal R, Ranganathan P. Study designs: Part 2 - Descriptive studies .  Perspect Clin Res . 2019;10(1):34-36. doi:10.4103/picr.PICR_154_18

Nevid J. Psychology: Concepts and Applications. Wadworth, 2013.

By Kendra Cherry, MSEd Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

Want to create or adapt books like this? Learn more about how Pressbooks supports open publishing practices.

Inferential Statistics

58 Some Basic Null Hypothesis Tests

Learning objectives.

• Conduct and interpret one-sample, dependent-samples, and independent-samples  t-  tests.
• Interpret the results of one-way, repeated measures, and factorial ANOVAs.
• Conduct and interpret null hypothesis tests of Pearson’s  r .

In this section, we look at several common null hypothesis testing procedures. The emphasis here is on providing enough information to allow you to conduct and interpret the most basic versions. In most cases, the online statistical analysis tools mentioned in Chapter 12 will handle the computations—as will programs such as Microsoft Excel and SPSS.

The  t- Test

As we have seen throughout this book, many studies in psychology focus on the difference between two means. The most common null hypothesis test for this type of statistical relationship is the  t- test . In this section, we look at three types of  t  tests that are used for slightly different research designs: the one-sample  t- test, the dependent-samples  t-  test, and the independent-samples  t- test. You may have already taken a course in statistics, but we will refresh your statistical

One-Sample  t- Test

The  one-sample  t- test  is used to compare a sample mean ( M ) with a hypothetical population mean (μ 0 ) that provides some interesting standard of comparison. The null hypothesis is that the mean for the population (µ) is equal to the hypothetical population mean: μ = μ 0 . The alternative hypothesis is that the mean for the population is different from the hypothetical population mean: μ ≠ μ 0 . To decide between these two hypotheses, we need to find the probability of obtaining the sample mean (or one more extreme) if the null hypothesis were true. But finding this  p  value requires first computing a test statistic called  t . (A test statistic  is a statistic that is computed only to help find the  p  value.) The formula for  t  is as follows:

[latex]t=\dfrac{{M -µ{_0}}}{\left(\dfrac{SD}{\sqrt N}\right)}[/latex]

Again, M  is the sample mean and µ 0  is the hypothetical population mean of interest.  SD  is the sample standard deviation and  N  is the sample size.

The reason the  t  statistic (or any test statistic) is useful is that we know how it is distributed when the null hypothesis is true. As shown in Figure 13.1, this distribution is unimodal and symmetrical, and it has a mean of 0. Its precise shape depends on a statistical concept called the degrees of freedom, which for a one-sample  t -test is  N  − 1. (There are 24 degrees of freedom for the distribution shown in Figure 13.1.) The important point is that knowing this distribution makes it possible to find the  p value for any  t  score. Consider, for example, a  t  score of 1.50 based on a sample of 25. The probability of a  t  score at least this extreme is given by the proportion of  t  scores in the distribution that are at least this extreme. For now, let us define  extreme  as being far from zero in either direction. Thus the  p  value is the proportion of  t  scores that are 1.50 or above  or  that are −1.50 or below—a value that turns out to be .14.

Fortunately, we do not have to deal directly with the distribution of  t  scores. If we were to enter our sample data and hypothetical mean of interest into one of the online statistical tools in Chapter 12 or into a program like SPSS (Excel does not have a one-sample  t- test function), the output would include both the  t  score and the  p  value. At this point, the rest of the procedure is simple. If  p  is equal to or less than .05, we reject the null hypothesis and conclude that the population mean differs from the hypothetical mean of interest. If  p  is greater than .05, we retain the null hypothesis and conclude that there is not enough evidence to say that the population mean differs from the hypothetical mean of interest. (Again, technically, we conclude only that we do not have enough evidence to conclude that it  does  differ.)

If we were to compute the  t  score by hand, we could use a table like Table 13.2 to make the decision. This table does not provide actual  p  values. Instead, it provides the  critical values  of  t  for different degrees of freedom ( df)  when α is .05. For now, let us focus on the two-tailed critical values in the last column of the table. Each of these values should be interpreted as a pair of values: one positive and one negative. For example, the two-tailed critical values when there are 24 degrees of freedom are 2.064 and −2.064. These are represented by the red vertical lines in Figure 13.1. The idea is that any  t  score below the lower critical value (the left-hand red line in Figure 13.1) is in the lowest 2.5% of the distribution, while any  t  score above the upper critical value (the right-hand red line) is in the highest 2.5% of the distribution. Therefore any  t  score beyond the critical value in  either  direction is in the most extreme 5% of  t  scores when the null hypothesis is true and has a  p  value less than .05. Thus if the  t  score we compute is beyond the critical value in either direction, then we reject the null hypothesis. If the  t  score we compute is between the upper and lower critical values, then we retain the null hypothesis.

Thus far, we have considered what is called a  two-tailed test , where we reject the null hypothesis if the  t  score for the sample is extreme in either direction. This test makes sense when we believe that the sample mean might differ from the hypothetical population mean but we do not have good reason to expect the difference to go in a particular direction. But it is also possible to do a  one-tailed test , where we reject the null hypothesis only if the  t  score for the sample is extreme in one direction that we specify before collecting the data. This test makes sense when we have good reason to expect the sample mean will differ from the hypothetical population mean in a particular direction.

Here is how it works. Each one-tailed critical value in Table 13.2 can again be interpreted as a pair of values: one positive and one negative. A  t  score below the lower critical value is in the lowest 5% of the distribution, and a  t  score above the upper critical value is in the highest 5% of the distribution. For 24 degrees of freedom, these values are −1.711 and 1.711. (These are represented by the green vertical lines in Figure 13.1.) However, for a one-tailed test, we must decide before collecting data whether we expect the sample mean to be lower than the hypothetical population mean, in which case we would use only the lower critical value, or we expect the sample mean to be greater than the hypothetical population mean, in which case we would use only the upper critical value. Notice that we still reject the null hypothesis when the  t  score for our sample is in the most extreme 5% of the t scores we would expect if the null hypothesis were true—so α remains at .05. We have simply redefined  extreme  to refer only to one tail of the distribution. The advantage of the one-tailed test is that critical values are less extreme. If the sample mean differs from the hypothetical population mean in the expected direction, then we have a better chance of rejecting the null hypothesis. The disadvantage is that if the sample mean differs from the hypothetical population mean in the unexpected direction, then there is no chance at all of rejecting the null hypothesis.

Example One-Sample  t – Test

Imagine that a health psychologist is interested in the accuracy of university students’ estimates of the number of calories in a chocolate chip cookie. He shows the cookie to a sample of 10 students and asks each one to estimate the number of calories in it. Because the actual number of calories in the cookie is 250, this is the hypothetical population mean of interest (µ 0 ). The null hypothesis is that the mean estimate for the population (μ) is 250. Because he has no real sense of whether the students will underestimate or overestimate the number of calories, he decides to do a two-tailed test. Now imagine further that the participants’ actual estimates are as follows:

250, 280, 200, 150, 175, 200, 200, 220, 180, 250.

The mean estimate for the sample ( M ) is 212.00 calories and the standard deviation ( SD ) is 39.17. The health psychologist can now compute the  t  score for his sample:

[latex]t=\dfrac{{212-250}}{\left(\dfrac{39.17}{\sqrt10}\right)}=-3.07[/latex]

If he enters the data into one of the online analysis tools or uses SPSS, it would also tell him that the two-tailed p  value for this  t  score (with 10 − 1 = 9 degrees of freedom) is .013. Because this is less than .05, the health psychologist would reject the null hypothesis and conclude that university students tend to underestimate the number of calories in a chocolate chip cookie. If he computes the  t  score by hand, he could look at Table 13.2 and see that the critical value of  t  for a two-tailed test with 9 degrees of freedom is ±2.262. The fact that his  t  score was more extreme than this critical value would tell him that his  p  value is less than .05 and that he should reject the null hypothesis. Using APA style, these results would be reported as follows:  t (9) = -3.07,  p  = .01. Note that the  t  and  p  are italicized, the degrees of freedom appear in brackets with no decimal remainder, and the values of  t  and  p  are rounded to two decimal places.

Finally, if this researcher had gone into this study with good reason to expect that university students underestimate the number of calories, then he could have done a one-tailed test instead of a two-tailed test. The only thing this decision would change is the critical value, which would be −1.833. This slightly less extreme value would make it a bit easier to reject the null hypothesis. However, if it turned out that university students overestimate the number of calories—no matter how much they overestimate it—the researcher would not have been able to reject the null hypothesis.

The Dependent-Samples  t – Test

The  dependent-samples  t -test  (sometimes called the paired-samples  t- test) is used to compare two means for the same sample tested at two different times or under two different conditions. This comparison is appropriate for pretest-posttest designs or within-subjects experiments. The null hypothesis is that the means at the two times or under the two conditions are the same in the population. The alternative hypothesis is that they are not the same. This test can also be one-tailed if the researcher has good reason to expect the difference goes in a particular direction.

It helps to think of the dependent-samples  t- test as a special case of the one-sample  t- test. However, the first step in the dependent-samples  t- test is to reduce the two scores for each participant to a single  difference score  by taking the difference between them. At this point, the dependent-samples  t- test becomes a one-sample  t- test on the difference scores. The hypothetical population mean (µ 0 ) of interest is 0 because this is what the mean difference score would be if there were no difference on average between the two times or two conditions. We can now think of the null hypothesis as being that the mean difference score in the population is 0 (µ 0  = 0) and the alternative hypothesis as being that the mean difference score in the population is not 0 (µ 0  ≠ 0).

Example Dependent-Samples  t – Test

Imagine that the health psychologist now knows that people tend to underestimate the number of calories in junk food and has developed a short training program to improve their estimates. To test the effectiveness of this program, he conducts a pretest-posttest study in which 10 participants estimate the number of calories in a chocolate chip cookie before the training program and then again afterward. Because he expects the program to increase the participants’ estimates, he decides to do a one-tailed test. Now imagine further that the pretest estimates are

230, 250, 280, 175, 150, 200, 180, 210, 220, 190

and that the posttest estimates (for the same participants in the same order) are

250, 260, 250, 200, 160, 200, 200, 180, 230, 240.

The difference scores, then, are as follows:

20, 10, −30, 25, 10, 0, 20, −30, 10, 50.

Note that it does not matter whether the first set of scores is subtracted from the second or the second from the first as long as it is done the same way for all participants. In this example, it makes sense to subtract the pretest estimates from the posttest estimates so that positive difference scores mean that the estimates went up after the training and negative difference scores mean the estimates went down.

The mean of the difference scores is 8.50 with a standard deviation of 27.27. The health psychologist can now compute the  t  score for his sample as follows:

[latex]t=\dfrac{{8.5-0}}{\left(\dfrac{27.27}{\sqrt10}\right)}=1.11[/latex]

If he enters the data into one of the online analysis tools or uses Excel or SPSS, it would tell him that the one-tailed  p  value for this  t  score (again with 10 − 1 = 9 degrees of freedom) is .148. Because this is greater than .05, he would retain the null hypothesis and conclude that the training program does not significantly increase people’s calorie estimates. If he were to compute the  t  score by hand, he could look at Table 13.2 and see that the critical value of  t for a one-tailed test with 9 degrees of freedom is 1.833. (It is positive this time because he was expecting a positive mean difference score.) The fact that his  t score was less extreme than this critical value would tell him that his  p  value is greater than .05 and that he should fail to reject the null hypothesis.

The Independent-Samples  t- Test

The  independent-samples  t- test  is used to compare the means of two separate samples ( M 1  and  M 2 ). The two samples might have been tested under different conditions in a between-subjects experiment, or they could be pre-existing groups in a cross-sectional design (e.g., women and men, extraverts and introverts). The null hypothesis is that the means of the two populations are the same: µ 1  = µ 2 . The alternative hypothesis is that they are not the same: µ 1  ≠ µ 2 . Again, the test can be one-tailed if the researcher has good reason to expect the difference goes in a particular direction.

The  t  statistic here is a bit more complicated because it must take into account two sample means, two standard deviations, and two sample sizes. The formula is as follows:

[latex]t=\dfrac{{M{_1}-M{_2}}}{\sqrt{\dfrac{SD{^2}{_1}}{n{_1}}+\dfrac{SD{^2}{_2}}{n{_2}}}}[/latex]

Notice that this formula includes squared standard deviations (the variances) that appear inside the square root symbol. Also, lowercase  n 1  and  n 2  refer to the sample sizes in the two groups or condition (as opposed to capital  N , which generally refers to the total sample size). The only additional thing to know here is that there are  N  − 2 degrees of freedom for the independent-samples  t-  test.

Example Independent-Samples  t – Test

Now the health psychologist wants to compare the calorie estimates of people who regularly eat junk food with the estimates of people who rarely eat junk food. He believes the difference could come out in either direction so he decides to conduct a two-tailed test. He collects data from a sample of eight participants who eat junk food regularly and seven participants who rarely eat junk food. The data are as follows:

Junk food eaters: 180, 220, 150, 85, 200, 170, 150, 190

Non–junk food eaters: 200, 240, 190, 175, 200, 300, 240

The mean for the non-junk food eaters is 220.71 with a standard deviation of 41.23. The mean for the junk food eaters is 168.12 with a standard deviation of 42.66. He can now compute his  t  score as follows:

[latex]t=\dfrac{{220.71-168.12}}{\sqrt{\dfrac{41.23{^2}}{8}+\dfrac{42.66{^2}}{7}}}= 2.42[/latex]

If he enters the data into one of the online analysis tools or uses Excel or SPSS, it would tell him that the two-tailed  p  value for this  t  score (with 15 − 2 = 13 degrees of freedom) is .015. Because this p value is less than .05, the health psychologist would reject the null hypothesis and conclude that people who eat junk food regularly make lower calorie estimates than people who eat it rarely. If he were to compute the  t  score by hand, he could look at Table 13.2 and see that the critical value of  t  for a two-tailed test with 13 degrees of freedom is ±2.160. The fact that his  t  score was more extreme than this critical value would tell him that his  p  value is less than .05 and that he should reject the null hypothesis.

The Analysis of Variance

T -tests are used to compare two means (a sample mean with a population mean, the means of two conditions or two groups). When there are more than two groups or condition means to be compared, the most common null hypothesis test is the  analysis of variance (ANOVA) . In this section, we look primarily at the  one-way ANOVA , which is used for between-subjects designs with a single independent variable. We then briefly consider some other versions of the ANOVA that are used for within-subjects and factorial research designs.

One-Way ANOVA

The one-way ANOVA is used to compare the means of more than two samples ( M 1 ,  M 2 … M G ) in a between-subjects design. The null hypothesis is that all the means are equal in the population: µ 1 = µ 2  =…= µ G . The alternative hypothesis is that not all the means in the population are equal.

The test statistic for the ANOVA is called  F . It is a ratio of two estimates of the population variance based on the sample data. One estimate of the population variance is called the  mean squares between groups (MS B )  and is based on the differences among the sample means. The other is called the mean squares within groups (MS W )  and is based on the differences among the scores within each group. The  F  statistic is the ratio of the  MS B  to the  MS W and can, therefore, be expressed as follows:

F = MS B / MS W

Again, the reason that  F  is useful is that we know how it is distributed when the null hypothesis is true. As shown in Figure 13.2, this distribution is unimodal and positively skewed with values that cluster around 1. The precise shape of the distribution depends on both the number of groups and the sample size, and there are degrees of freedom values associated with each of these. The between-groups degrees of freedom is the number of groups minus one:  df B  = ( G  − 1). The within-groups degrees of freedom is the total sample size minus the number of groups:  df W  =  N  −  G . Again, knowing the distribution of  F when the null hypothesis is true allows us to find the  p  value.

The online tools in Chapter 12 and statistical software such as Excel and SPSS will compute  F  and find the  p  value. If  p  is equal to or less than .05, then we reject the null hypothesis and conclude that there are differences among the group means in the population. If  p  is greater than .05, then we retain the null hypothesis and conclude that there is not enough evidence to say that there are differences. In the unlikely event that we would compute  F  by hand, we can use a table of critical values like Table 13.3 “Table of Critical Values of ” to make the decision. The idea is that any  F  ratio greater than the critical value has a  p value of less than .05. Thus if the  F  ratio we compute is beyond the critical value, then we reject the null hypothesis. If the F ratio we compute is less than the critical value, then we retain the null hypothesis.

Example One-Way ANOVA

Imagine that the health psychologist wants to compare the calorie estimates of psychology majors, nutrition majors, and professional dieticians. He collects the following data:

Psych majors: 200, 180, 220, 160, 150, 200, 190, 200

Nutrition majors: 190, 220, 200, 230, 160, 150, 200, 210, 195

Dieticians: 220, 250, 240, 275, 250, 230, 200, 240

The means are 187.50 ( SD  = 23.14), 195.00 ( SD  = 27.77), and 238.13 ( SD  = 22.35), respectively. So it appears that dieticians made substantially more accurate estimates on average. The researcher would almost certainly enter these data into a program such as Excel or SPSS, which would compute  F  for him or her and find the  p  value. Table 13.4 shows the output of the one-way ANOVA function in Excel for these data. This table is referred to as an ANOVA table. It shows that  MS B  is 5,971.88,  MS W  is 602.23, and their ratio,  F , is 9.92. The  p  value is .0009. Because this value is below .05, the researcher would reject the null hypothesis and conclude that the mean calorie estimates for the three groups are not the same in the population. Notice that the ANOVA table also includes the “sum of squares” ( SS ) for between groups and for within groups. These values are computed on the way to finding  MS B  and MS W  but are not typically reported by the researcher. Finally, if the researcher were to compute the  F  ratio by hand, he could look at Table 13.3 and see that the critical value of  F  with 2 and 21 degrees of freedom is 3.467 (the same value in Table 13.4 under  F crit ). The fact that his  F  score was more extreme than this critical value would tell him that his  p  value is less than .05 and that he should reject the null hypothesis.

ANOVA Elaborations

Post hoc comparisons.

When we reject the null hypothesis in a one-way ANOVA, we conclude that the group means are not all the same in the population. But this can indicate different things. With three groups, it can indicate that all three means are significantly different from each other. Or it can indicate that one of the means is significantly different from the other two, but the other two are not significantly different from each other. It could be, for example, that the mean calorie estimates of psychology majors, nutrition majors, and dieticians are all significantly different from each other. Or it could be that the mean for dieticians is significantly different from the means for psychology and nutrition majors, but the means for psychology and nutrition majors are not significantly different from each other. For this reason, statistically significant one-way ANOVA results are typically followed up with a series of  post hoc comparisons  of selected pairs of group means to determine which are different from which others.

One approach to post hoc comparisons would be to conduct a series of independent-samples  t- tests comparing each group mean to each of the other group means. But there is a problem with this approach. In general, if we conduct a  t -test when the null hypothesis is true, we have a 5% chance of mistakenly rejecting the null hypothesis (see Section 13.3 “Additional Considerations” for more on such Type I errors). If we conduct several  t- tests when the null hypothesis is true, the chance of mistakenly rejecting  at least one null hypothesis increases with each test we conduct. Thus researchers do not usually make post hoc comparisons using standard  t- tests because there is too great a chance that they will mistakenly reject at least one null hypothesis. Instead, they use one of several modified  t -test procedures—among them the Bonferonni procedure, Fisher’s least significant difference (LSD) test, and Tukey’s honestly significant difference (HSD) test. The details of these approaches are beyond the scope of this book, but it is important to understand their purpose. It is to keep the risk of mistakenly rejecting a true null hypothesis to an acceptable level (close to 5%).

Repeated-Measures ANOVA

Recall that the one-way ANOVA is appropriate for between-subjects designs in which the means being compared come from separate groups of participants. It is not appropriate for within-subjects designs in which the means being compared come from the same participants tested under different conditions or at different times. This requires a slightly different approach, called the repeated-measures ANOVA . The basics of the repeated-measures ANOVA are the same as for the one-way ANOVA. The main difference is that measuring the dependent variable multiple times for each participant allows for a more refined measure of  MS W . Imagine, for example, that the dependent variable in a study is a measure of reaction time. Some participants will be faster or slower than others because of stable individual differences in their nervous systems, muscles, and other factors. In a between-subjects design, these stable individual differences would simply add to the variability within the groups and increase the value of  MS W (which would, in turn, decrease the value of F). In a within-subjects design, however, these stable individual differences can be measured and subtracted from the value of  MS W . This lower value of  MS W  means a higher value of  F  and a more sensitive test.

Factorial ANOVA

When more than one independent variable is included in a factorial design, the appropriate approach is the  factorial ANOVA . Again, the basics of the factorial ANOVA are the same as for the one-way and repeated-measures ANOVAs. The main difference is that it produces an  F  ratio and  p  value for each main effect and for each interaction. Returning to our calorie estimation example, imagine that the health psychologist tests the effect of participant major (psychology vs. nutrition) and food type (cookie vs. hamburger) in a factorial design. A factorial ANOVA would produce separate  F  ratios and  p values for the main effect of major, the main effect of food type, and the interaction between major and food. Appropriate modifications must be made depending on whether the design is between-subjects, within-subjects, or mixed.

Testing Correlation Coefficients

For relationships between quantitative variables, where Pearson’s  r (the correlation coefficient)   is used to describe the strength of those relationships, the appropriate null hypothesis test is a test of the correlation coefficient. The basic logic is exactly the same as for other null hypothesis tests. In this case, the null hypothesis is that there is no relationship in the population. We can use the Greek lowercase rho (ρ) to represent the relevant parameter: ρ = 0. The alternative hypothesis is that there is a relationship in the population: ρ ≠ 0. As with the  t-  test, this test can be two-tailed if the researcher has no expectation about the direction of the relationship or one-tailed if the researcher expects the relationship to go in a particular direction.

It is possible to use the correlation coefficient for the sample to compute a  t  score with  N  − 2 degrees of freedom and then to proceed as for a  t- test. However, because of the way it is computed, the correlation coefficient can also be treated as its own test statistic. The online statistical tools and statistical software such as Excel and SPSS generally compute the correlation coefficient and provide the  p  value associated with that value. As always, if the  p  value is equal to or less than .05, we reject the null hypothesis and conclude that there is a relationship between the variables in the population. If the  p  value is greater than .05, we retain the null hypothesis and conclude that there is not enough evidence to say there is a relationship in the population. If we compute the correlation coefficient by hand, we can use a table like Table 13.5, which shows the critical values of  r  for various samples sizes when α is .05. A sample value of the correlation coefficient that is more extreme than the critical value is statistically significant.

Example Test of a Correlation Coefficient

Imagine that the health psychologist is interested in the correlation between people’s calorie estimates and their weight. She has no expectation about the direction of the relationship, so she decides to conduct a two-tailed test. She computes the correlation coefficient for a sample of 22 university students and finds that Pearson’s  r  is −.21. The statistical software she uses tells her that the  p  value is .348. It is greater than .05, so she retains the null hypothesis and concludes that there is no relationship between people’s calorie estimates and their weight. If she were to compute the correlation coefficient by hand, she could look at Table 13.5 and see that the critical value for 22 − 2 = 20 degrees of freedom is .444. The fact that the correlation coefficient for her sample is less extreme than this critical value tells her that the  p  value is greater than .05 and that she should retain the null hypothesis.

A test that involves looking at the difference between two means.

Used to compare a sample mean (M) with a hypothetical population mean (μ0) that provides some interesting standard of comparison.

A statistic (e.g., F , t , etc.) that is computed to compare against what is expected in the null hypothesis, and thus helps find the p value.

The absolute value that a test statistic (e.g., F , t , etc.) must exceed to be considered statistically significant.

Where we reject the null hypothesis if the test statistic for the sample is extreme in either direction (+/-).

Where we reject the null hypothesis only if the  t  score for the sample is extreme in one direction that we specify before collecting the data.

Used to compare two means for the same sample tested at two different times or under two different conditions (sometimes called the paired-samples  t -test).

A method to reduce pairs of scores (e.g., pre- and post-test) to a single score by calculating the difference between them.

Used to compare the means of two separate samples (M1 and M2).

A statistical test used when there are more than two groups or condition means to be compared.

Used for between-subjects designs with a single independent variable.

An estimate of the population variance and is based on the differences among the sample means.

An estimate of the population variance and is based on the differences among the scores within each group.

An unplanned (not hypothesized) test of which pairs of group mean scores are different from which others.

Compares the means from the same participants tested under different conditions or at different times in which the dependent variable is measured multiple times for each participant.

A statistical method to detect differences in the means between conditions when there are two or more independent variables in a factorial design. It allows the detection of main effects and interaction effects.

Research Methods in Psychology Copyright © 2019 by Rajiv S. Jhangiani, I-Chant A. Chiang, Carrie Cuttler, & Dana C. Leighton is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License , except where otherwise noted.

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13.1 Understanding Null Hypothesis Testing

Learning objectives.

• Explain the purpose of null hypothesis testing, including the role of sampling error.
• Describe the basic logic of null hypothesis testing.
• Describe the role of relationship strength and sample size in determining statistical significance and make reasonable judgments about statistical significance based on these two factors.

  The Purpose of Null Hypothesis Testing

As we have seen, psychological research typically involves measuring one or more variables in a sample and computing descriptive statistics for that sample. In general, however, the researcher’s goal is not to draw conclusions about that sample but to draw conclusions about the population that the sample was selected from. Thus researchers must use sample statistics to draw conclusions about the corresponding values in the population. These corresponding values in the population are called  parameters . Imagine, for example, that a researcher measures the number of depressive symptoms exhibited by each of 50 adults with clinical depression and computes the mean number of symptoms. The researcher probably wants to use this sample statistic (the mean number of symptoms for the sample) to draw conclusions about the corresponding population parameter (the mean number of symptoms for adults with clinical depression).

Unfortunately, sample statistics are not perfect estimates of their corresponding population parameters. This is because there is a certain amount of random variability in any statistic from sample to sample. The mean number of depressive symptoms might be 8.73 in one sample of adults with clinical depression, 6.45 in a second sample, and 9.44 in a third—even though these samples are selected randomly from the same population. Similarly, the correlation (Pearson’s  r ) between two variables might be +.24 in one sample, −.04 in a second sample, and +.15 in a third—again, even though these samples are selected randomly from the same population. This random variability in a statistic from sample to sample is called  sampling error . (Note that the term error  here refers to random variability and does not imply that anyone has made a mistake. No one “commits a sampling error.”)

One implication of this is that when there is a statistical relationship in a sample, it is not always clear that there is a statistical relationship in the population. A small difference between two group means in a sample might indicate that there is a small difference between the two group means in the population. But it could also be that there is no difference between the means in the population and that the difference in the sample is just a matter of sampling error. Similarly, a Pearson’s  r  value of −.29 in a sample might mean that there is a negative relationship in the population. But it could also be that there is no relationship in the population and that the relationship in the sample is just a matter of sampling error.

In fact, any statistical relationship in a sample can be interpreted in two ways:

• There is a relationship in the population, and the relationship in the sample reflects this.
• There is no relationship in the population, and the relationship in the sample reflects only sampling error.

The purpose of null hypothesis testing is simply to help researchers decide between these two interpretations.

The Logic of Null Hypothesis Testing

Null hypothesis testing  is a formal approach to deciding between two interpretations of a statistical relationship in a sample. One interpretation is called the  null hypothesis  (often symbolized  H 0  and read as “H-naught”). This is the idea that there is no relationship in the population and that the relationship in the sample reflects only sampling error. Informally, the null hypothesis is that the sample relationship “occurred by chance.” The other interpretation is called the  alternative hypothesis  (often symbolized as  H 1 ). This is the idea that there is a relationship in the population and that the relationship in the sample reflects this relationship in the population.

Again, every statistical relationship in a sample can be interpreted in either of these two ways: It might have occurred by chance, or it might reflect a relationship in the population. So researchers need a way to decide between them. Although there are many specific null hypothesis testing techniques, they are all based on the same general logic. The steps are as follows:

• Assume for the moment that the null hypothesis is true. There is no relationship between the variables in the population.
• Determine how likely the sample relationship would be if the null hypothesis were true.
• If the sample relationship would be extremely unlikely, then reject the null hypothesis  in favor of the alternative hypothesis. If it would not be extremely unlikely, then  retain the null hypothesis .

Following this logic, we can begin to understand why Mehl and his colleagues concluded that there is no difference in talkativeness between women and men in the population. In essence, they asked the following question: “If there were no difference in the population, how likely is it that we would find a small difference of  d  = 0.06 in our sample?” Their answer to this question was that this sample relationship would be fairly likely if the null hypothesis were true. Therefore, they retained the null hypothesis—concluding that there is no evidence of a sex difference in the population. We can also see why Kanner and his colleagues concluded that there is a correlation between hassles and symptoms in the population. They asked, “If the null hypothesis were true, how likely is it that we would find a strong correlation of +.60 in our sample?” Their answer to this question was that this sample relationship would be fairly unlikely if the null hypothesis were true. Therefore, they rejected the null hypothesis in favor of the alternative hypothesis—concluding that there is a positive correlation between these variables in the population.

A crucial step in null hypothesis testing is finding the likelihood of the sample result if the null hypothesis were true. This probability is called the  p value . A low  p  value means that the sample result would be unlikely if the null hypothesis were true and leads to the rejection of the null hypothesis. A p  value that is not low means that the sample result would be likely if the null hypothesis were true and leads to the retention of the null hypothesis. But how low must the  p  value be before the sample result is considered unlikely enough to reject the null hypothesis? In null hypothesis testing, this criterion is called  α (alpha)  and is almost always set to .05. If there is a 5% chance or less of a result as extreme as the sample result if the null hypothesis were true, then the null hypothesis is rejected. When this happens, the result is said to be  statistically significant . If there is greater than a 5% chance of a result as extreme as the sample result when the null hypothesis is true, then the null hypothesis is retained. This does not necessarily mean that the researcher accepts the null hypothesis as true—only that there is not currently enough evidence to reject it. Researchers often use the expression “fail to reject the null hypothesis” rather than “retain the null hypothesis,” but they never use the expression “accept the null hypothesis.”

The Misunderstood  p  Value

The  p  value is one of the most misunderstood quantities in psychological research (Cohen, 1994) [1] . Even professional researchers misinterpret it, and it is not unusual for such misinterpretations to appear in statistics textbooks!

The most common misinterpretation is that the  p  value is the probability that the null hypothesis is true—that the sample result occurred by chance. For example, a misguided researcher might say that because the  p  value is .02, there is only a 2% chance that the result is due to chance and a 98% chance that it reflects a real relationship in the population. But this is incorrect . The  p  value is really the probability of a result at least as extreme as the sample result  if  the null hypothesis  were  true. So a  p  value of .02 means that if the null hypothesis were true, a sample result this extreme would occur only 2% of the time.

You can avoid this misunderstanding by remembering that the  p  value is not the probability that any particular  hypothesis  is true or false. Instead, it is the probability of obtaining the  sample result  if the null hypothesis were true.

“Null Hypothesis” retrieved from http://imgs.xkcd.com/comics/null_hypothesis.png (CC-BY-NC 2.5)

Role of Sample Size and Relationship Strength

Recall that null hypothesis testing involves answering the question, “If the null hypothesis were true, what is the probability of a sample result as extreme as this one?” In other words, “What is the  p  value?” It can be helpful to see that the answer to this question depends on just two considerations: the strength of the relationship and the size of the sample. Specifically, the stronger the sample relationship and the larger the sample, the less likely the result would be if the null hypothesis were true. That is, the lower the  p  value. This should make sense. Imagine a study in which a sample of 500 women is compared with a sample of 500 men in terms of some psychological characteristic, and Cohen’s  d  is a strong 0.50. If there were really no sex difference in the population, then a result this strong based on such a large sample should seem highly unlikely. Now imagine a similar study in which a sample of three women is compared with a sample of three men, and Cohen’s  d  is a weak 0.10. If there were no sex difference in the population, then a relationship this weak based on such a small sample should seem likely. And this is precisely why the null hypothesis would be rejected in the first example and retained in the second.

Of course, sometimes the result can be weak and the sample large, or the result can be strong and the sample small. In these cases, the two considerations trade off against each other so that a weak result can be statistically significant if the sample is large enough and a strong relationship can be statistically significant even if the sample is small. Table 13.1 shows roughly how relationship strength and sample size combine to determine whether a sample result is statistically significant. The columns of the table represent the three levels of relationship strength: weak, medium, and strong. The rows represent four sample sizes that can be considered small, medium, large, and extra large in the context of psychological research. Thus each cell in the table represents a combination of relationship strength and sample size. If a cell contains the word  Yes , then this combination would be statistically significant for both Cohen’s  d  and Pearson’s  r . If it contains the word  No , then it would not be statistically significant for either. There is one cell where the decision for  d  and  r  would be different and another where it might be different depending on some additional considerations, which are discussed in Section 13.2 “Some Basic Null Hypothesis Tests”

Although Table 13.1 provides only a rough guideline, it shows very clearly that weak relationships based on medium or small samples are never statistically significant and that strong relationships based on medium or larger samples are always statistically significant. If you keep this lesson in mind, you will often know whether a result is statistically significant based on the descriptive statistics alone. It is extremely useful to be able to develop this kind of intuitive judgment. One reason is that it allows you to develop expectations about how your formal null hypothesis tests are going to come out, which in turn allows you to detect problems in your analyses. For example, if your sample relationship is strong and your sample is medium, then you would expect to reject the null hypothesis. If for some reason your formal null hypothesis test indicates otherwise, then you need to double-check your computations and interpretations. A second reason is that the ability to make this kind of intuitive judgment is an indication that you understand the basic logic of this approach in addition to being able to do the computations.

Statistical Significance Versus Practical Significance

Table 13.1 illustrates another extremely important point. A statistically significant result is not necessarily a strong one. Even a very weak result can be statistically significant if it is based on a large enough sample. This is closely related to Janet Shibley Hyde’s argument about sex differences (Hyde, 2007) [2] . The differences between women and men in mathematical problem solving and leadership ability are statistically significant. But the word  significant  can cause people to interpret these differences as strong and important—perhaps even important enough to influence the college courses they take or even who they vote for. As we have seen, however, these statistically significant differences are actually quite weak—perhaps even “trivial.”

This is why it is important to distinguish between the  statistical  significance of a result and the  practical  significance of that result.  Practical significance refers to the importance or usefulness of the result in some real-world context. Many sex differences are statistically significant—and may even be interesting for purely scientific reasons—but they are not practically significant. In clinical practice, this same concept is often referred to as “clinical significance.” For example, a study on a new treatment for social phobia might show that it produces a statistically significant positive effect. Yet this effect still might not be strong enough to justify the time, effort, and other costs of putting it into practice—especially if easier and cheaper treatments that work almost as well already exist. Although statistically significant, this result would be said to lack practical or clinical significance.

“Conditional Risk” retrieved from http://imgs.xkcd.com/comics/conditional_risk.png (CC-BY-NC 2.5)

Key Takeaways

• Null hypothesis testing is a formal approach to deciding whether a statistical relationship in a sample reflects a real relationship in the population or is just due to chance.
• The logic of null hypothesis testing involves assuming that the null hypothesis is true, finding how likely the sample result would be if this assumption were correct, and then making a decision. If the sample result would be unlikely if the null hypothesis were true, then it is rejected in favor of the alternative hypothesis. If it would not be unlikely, then the null hypothesis is retained.
• The probability of obtaining the sample result if the null hypothesis were true (the  p  value) is based on two considerations: relationship strength and sample size. Reasonable judgments about whether a sample relationship is statistically significant can often be made by quickly considering these two factors.
• Statistical significance is not the same as relationship strength or importance. Even weak relationships can be statistically significant if the sample size is large enough. It is important to consider relationship strength and the practical significance of a result in addition to its statistical significance.
• Discussion: Imagine a study showing that people who eat more broccoli tend to be happier. Explain for someone who knows nothing about statistics why the researchers would conduct a null hypothesis test.
• The correlation between two variables is  r  = −.78 based on a sample size of 137.
• The mean score on a psychological characteristic for women is 25 ( SD  = 5) and the mean score for men is 24 ( SD  = 5). There were 12 women and 10 men in this study.
• In a memory experiment, the mean number of items recalled by the 40 participants in Condition A was 0.50 standard deviations greater than the mean number recalled by the 40 participants in Condition B.
• In another memory experiment, the mean scores for participants in Condition A and Condition B came out exactly the same!
• A student finds a correlation of  r  = .04 between the number of units the students in his research methods class are taking and the students’ level of stress.
• Cohen, J. (1994). The world is round: p < .05. American Psychologist, 49 , 997–1003. ↵
• Hyde, J. S. (2007). New directions in the study of gender similarities and differences. Current Directions in Psychological Science, 16 , 259–263. ↵

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Null hypothesis significance testing: a short tutorial

Cyril pernet.

1 Centre for Clinical Brain Sciences (CCBS), Neuroimaging Sciences, The University of Edinburgh, Edinburgh, UK

Version Changes

Revised. amendments from version 2.

This v3 includes minor changes that reflect the 3rd reviewers' comments - in particular the theoretical vs. practical difference between Fisher and Neyman-Pearson. Additional information and reference is also included regarding the interpretation of p-value for low powered studies.

Peer Review Summary

Although thoroughly criticized, null hypothesis significance testing (NHST) remains the statistical method of choice used to provide evidence for an effect, in biological, biomedical and social sciences. In this short tutorial, I first summarize the concepts behind the method, distinguishing test of significance (Fisher) and test of acceptance (Newman-Pearson) and point to common interpretation errors regarding the p-value. I then present the related concepts of confidence intervals and again point to common interpretation errors. Finally, I discuss what should be reported in which context. The goal is to clarify concepts to avoid interpretation errors and propose reporting practices.

The Null Hypothesis Significance Testing framework

NHST is a method of statistical inference by which an experimental factor is tested against a hypothesis of no effect or no relationship based on a given observation. The method is a combination of the concepts of significance testing developed by Fisher in 1925 and of acceptance based on critical rejection regions developed by Neyman & Pearson in 1928 . In the following I am first presenting each approach, highlighting the key differences and common misconceptions that result from their combination into the NHST framework (for a more mathematical comparison, along with the Bayesian method, see Christensen, 2005 ). I next present the related concept of confidence intervals. I finish by discussing practical aspects in using NHST and reporting practice.

Fisher, significance testing, and the p-value

The method developed by ( Fisher, 1934 ; Fisher, 1955 ; Fisher, 1959 ) allows to compute the probability of observing a result at least as extreme as a test statistic (e.g. t value), assuming the null hypothesis of no effect is true. This probability or p-value reflects (1) the conditional probability of achieving the observed outcome or larger: p(Obs≥t|H0), and (2) is therefore a cumulative probability rather than a point estimate. It is equal to the area under the null probability distribution curve from the observed test statistic to the tail of the null distribution ( Turkheimer et al. , 2004 ). The approach proposed is of ‘proof by contradiction’ ( Christensen, 2005 ), we pose the null model and test if data conform to it.

In practice, it is recommended to set a level of significance (a theoretical p-value) that acts as a reference point to identify significant results, that is to identify results that differ from the null-hypothesis of no effect. Fisher recommended using p=0.05 to judge whether an effect is significant or not as it is roughly two standard deviations away from the mean for the normal distribution ( Fisher, 1934 page 45: ‘The value for which p=.05, or 1 in 20, is 1.96 or nearly 2; it is convenient to take this point as a limit in judging whether a deviation is to be considered significant or not’). A key aspect of Fishers’ theory is that only the null-hypothesis is tested, and therefore p-values are meant to be used in a graded manner to decide whether the evidence is worth additional investigation and/or replication ( Fisher, 1971 page 13: ‘it is open to the experimenter to be more or less exacting in respect of the smallness of the probability he would require […]’ and ‘no isolated experiment, however significant in itself, can suffice for the experimental demonstration of any natural phenomenon’). How small the level of significance is, is thus left to researchers.

What is not a p-value? Common mistakes

The p-value is not an indication of the strength or magnitude of an effect . Any interpretation of the p-value in relation to the effect under study (strength, reliability, probability) is wrong, since p-values are conditioned on H0. In addition, while p-values are randomly distributed (if all the assumptions of the test are met) when there is no effect, their distribution depends of both the population effect size and the number of participants, making impossible to infer strength of effect from them.

Similarly, 1-p is not the probability to replicate an effect . Often, a small value of p is considered to mean a strong likelihood of getting the same results on another try, but again this cannot be obtained because the p-value is not informative on the effect itself ( Miller, 2009 ). Because the p-value depends on the number of subjects, it can only be used in high powered studies to interpret results. In low powered studies (typically small number of subjects), the p-value has a large variance across repeated samples, making it unreliable to estimate replication ( Halsey et al. , 2015 ).

A (small) p-value is not an indication favouring a given hypothesis . Because a low p-value only indicates a misfit of the null hypothesis to the data, it cannot be taken as evidence in favour of a specific alternative hypothesis more than any other possible alternatives such as measurement error and selection bias ( Gelman, 2013 ). Some authors have even argued that the more (a priori) implausible the alternative hypothesis, the greater the chance that a finding is a false alarm ( Krzywinski & Altman, 2013 ; Nuzzo, 2014 ).

The p-value is not the probability of the null hypothesis p(H0), of being true, ( Krzywinski & Altman, 2013 ). This common misconception arises from a confusion between the probability of an observation given the null p(Obs≥t|H0) and the probability of the null given an observation p(H0|Obs≥t) that is then taken as an indication for p(H0) (see Nickerson, 2000 ).

Neyman-Pearson, hypothesis testing, and the α-value

Neyman & Pearson (1933) proposed a framework of statistical inference for applied decision making and quality control. In such framework, two hypotheses are proposed: the null hypothesis of no effect and the alternative hypothesis of an effect, along with a control of the long run probabilities of making errors. The first key concept in this approach, is the establishment of an alternative hypothesis along with an a priori effect size. This differs markedly from Fisher who proposed a general approach for scientific inference conditioned on the null hypothesis only. The second key concept is the control of error rates . Neyman & Pearson (1928) introduced the notion of critical intervals, therefore dichotomizing the space of possible observations into correct vs. incorrect zones. This dichotomization allows distinguishing correct results (rejecting H0 when there is an effect and not rejecting H0 when there is no effect) from errors (rejecting H0 when there is no effect, the type I error, and not rejecting H0 when there is an effect, the type II error). In this context, alpha is the probability of committing a Type I error in the long run. Alternatively, Beta is the probability of committing a Type II error in the long run.

The (theoretical) difference in terms of hypothesis testing between Fisher and Neyman-Pearson is illustrated on Figure 1 . In the 1 st case, we choose a level of significance for observed data of 5%, and compute the p-value. If the p-value is below the level of significance, it is used to reject H0. In the 2 nd case, we set a critical interval based on the a priori effect size and error rates. If an observed statistic value is below and above the critical values (the bounds of the confidence region), it is deemed significantly different from H0. In the NHST framework, the level of significance is (in practice) assimilated to the alpha level, which appears as a simple decision rule: if the p-value is less or equal to alpha, the null is rejected. It is however a common mistake to assimilate these two concepts. The level of significance set for a given sample is not the same as the frequency of acceptance alpha found on repeated sampling because alpha (a point estimate) is meant to reflect the long run probability whilst the p-value (a cumulative estimate) reflects the current probability ( Fisher, 1955 ; Hubbard & Bayarri, 2003 ).

The figure was prepared with G-power for a one-sided one-sample t-test, with a sample size of 32 subjects, an effect size of 0.45, and error rates alpha=0.049 and beta=0.80. In Fisher’s procedure, only the nil-hypothesis is posed, and the observed p-value is compared to an a priori level of significance. If the observed p-value is below this level (here p=0.05), one rejects H0. In Neyman-Pearson’s procedure, the null and alternative hypotheses are specified along with an a priori level of acceptance. If the observed statistical value is outside the critical region (here [-∞ +1.69]), one rejects H0.

Acceptance or rejection of H0?

The acceptance level α can also be viewed as the maximum probability that a test statistic falls into the rejection region when the null hypothesis is true ( Johnson, 2013 ). Therefore, one can only reject the null hypothesis if the test statistics falls into the critical region(s), or fail to reject this hypothesis. In the latter case, all we can say is that no significant effect was observed, but one cannot conclude that the null hypothesis is true. This is another common mistake in using NHST: there is a profound difference between accepting the null hypothesis and simply failing to reject it ( Killeen, 2005 ). By failing to reject, we simply continue to assume that H0 is true, which implies that one cannot argue against a theory from a non-significant result (absence of evidence is not evidence of absence). To accept the null hypothesis, tests of equivalence ( Walker & Nowacki, 2011 ) or Bayesian approaches ( Dienes, 2014 ; Kruschke, 2011 ) must be used.

Confidence intervals

Confidence intervals (CI) are builds that fail to cover the true value at a rate of alpha, the Type I error rate ( Morey & Rouder, 2011 ) and therefore indicate if observed values can be rejected by a (two tailed) test with a given alpha. CI have been advocated as alternatives to p-values because (i) they allow judging the statistical significance and (ii) provide estimates of effect size. Assuming the CI (a)symmetry and width are correct (but see Wilcox, 2012 ), they also give some indication about the likelihood that a similar value can be observed in future studies. For future studies of the same sample size, 95% CI give about 83% chance of replication success ( Cumming & Maillardet, 2006 ). If sample sizes however differ between studies, CI do not however warranty any a priori coverage.

Although CI provide more information, they are not less subject to interpretation errors (see Savalei & Dunn, 2015 for a review). The most common mistake is to interpret CI as the probability that a parameter (e.g. the population mean) will fall in that interval X% of the time. The correct interpretation is that, for repeated measurements with the same sample sizes, taken from the same population, X% of times the CI obtained will contain the true parameter value ( Tan & Tan, 2010 ). The alpha value has the same interpretation as testing against H0, i.e. we accept that 1-alpha CI are wrong in alpha percent of the times in the long run. This implies that CI do not allow to make strong statements about the parameter of interest (e.g. the mean difference) or about H1 ( Hoekstra et al. , 2014 ). To make a statement about the probability of a parameter of interest (e.g. the probability of the mean), Bayesian intervals must be used.

The (correct) use of NHST

NHST has always been criticized, and yet is still used every day in scientific reports ( Nickerson, 2000 ). One question to ask oneself is what is the goal of a scientific experiment at hand? If the goal is to establish a discrepancy with the null hypothesis and/or establish a pattern of order, because both requires ruling out equivalence, then NHST is a good tool ( Frick, 1996 ; Walker & Nowacki, 2011 ). If the goal is to test the presence of an effect and/or establish some quantitative values related to an effect, then NHST is not the method of choice since testing is conditioned on H0.

While a Bayesian analysis is suited to estimate that the probability that a hypothesis is correct, like NHST, it does not prove a theory on itself, but adds its plausibility ( Lindley, 2000 ). No matter what testing procedure is used and how strong results are, ( Fisher, 1959 p13) reminds us that ‘ […] no isolated experiment, however significant in itself, can suffice for the experimental demonstration of any natural phenomenon'. Similarly, the recent statement of the American Statistical Association ( Wasserstein & Lazar, 2016 ) makes it clear that conclusions should be based on the researchers understanding of the problem in context, along with all summary data and tests, and that no single value (being p-values, Bayesian factor or else) can be used support or invalidate a theory.

What to report and how?

Considering that quantitative reports will always have more information content than binary (significant or not) reports, we can always argue that raw and/or normalized effect size, confidence intervals, or Bayes factor must be reported. Reporting everything can however hinder the communication of the main result(s), and we should aim at giving only the information needed, at least in the core of a manuscript. Here I propose to adopt optimal reporting in the result section to keep the message clear, but have detailed supplementary material. When the hypothesis is about the presence/absence or order of an effect, and providing that a study has sufficient power, NHST is appropriate and it is sufficient to report in the text the actual p-value since it conveys the information needed to rule out equivalence. When the hypothesis and/or the discussion involve some quantitative value, and because p-values do not inform on the effect, it is essential to report on effect sizes ( Lakens, 2013 ), preferably accompanied with confidence or credible intervals. The reasoning is simply that one cannot predict and/or discuss quantities without accounting for variability. For the reader to understand and fully appreciate the results, nothing else is needed.

Because science progress is obtained by cumulating evidence ( Rosenthal, 1991 ), scientists should also consider the secondary use of the data. With today’s electronic articles, there are no reasons for not including all of derived data: mean, standard deviations, effect size, CI, Bayes factor should always be included as supplementary tables (or even better also share raw data). It is also essential to report the context in which tests were performed – that is to report all of the tests performed (all t, F, p values) because of the increase type one error rate due to selective reporting (multiple comparisons and p-hacking problems - Ioannidis, 2005 ). Providing all of this information allows (i) other researchers to directly and effectively compare their results in quantitative terms (replication of effects beyond significance, Open Science Collaboration, 2015 ), (ii) to compute power to future studies ( Lakens & Evers, 2014 ), and (iii) to aggregate results for meta-analyses whilst minimizing publication bias ( van Assen et al. , 2014 ).

[version 3; referees: 1 approved

Funding Statement

The author(s) declared that no grants were involved in supporting this work.

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Referee response for version 3

Dorothy vera margaret bishop.

1 Department of Experimental Psychology, University of Oxford, Oxford, UK

I can see from the history of this paper that the author has already been very responsive to reviewer comments, and that the process of revising has now been quite protracted.

That makes me reluctant to suggest much more, but I do see potential here for making the paper more impactful. So my overall view is that, once a few typos are fixed (see below), this could be published as is, but I think there is an issue with the potential readership and that further revision could overcome this.

I suspect my take on this is rather different from other reviewers, as I do not regard myself as a statistics expert, though I am on the more quantitative end of the continuum of psychologists and I try to keep up to date. I think I am quite close to the target readership , insofar as I am someone who was taught about statistics ages ago and uses stats a lot, but never got adequate training in the kinds of topic covered by this paper. The fact that I am aware of controversies around the interpretation of confidence intervals etc is simply because I follow some discussions of this on social media. I am therefore very interested to have a clear account of these issues.

This paper contains helpful information for someone in this position, but it is not always clear, and I felt the relevance of some of the content was uncertain. So here are some recommendations:

• As one previous reviewer noted, it’s questionable that there is a need for a tutorial introduction, and the limited length of this article does not lend itself to a full explanation. So it might be better to just focus on explaining as clearly as possible the problems people have had in interpreting key concepts. I think a title that made it clear this was the content would be more appealing than the current one.
• P 3, col 1, para 3, last sentence. Although statisticians always emphasise the arbitrary nature of p < .05, we all know that in practice authors who use other values are likely to have their analyses queried. I wondered whether it would be useful here to note that in some disciplines different cutoffs are traditional, e.g. particle physics. Or you could cite David Colquhoun’s paper in which he recommends using p < .001 ( http://rsos.royalsocietypublishing.org/content/1/3/140216) - just to be clear that the traditional p < .05 has been challenged.

What I can’t work out is how you would explain the alpha from Neyman-Pearson in the same way (though I can see from Figure 1 that with N-P you could test an alternative hypothesis, such as the idea that the coin would be heads 75% of the time).

‘By failing to reject, we simply continue to assume that H0 is true, which implies that one cannot….’ have ‘In failing to reject, we do not assume that H0 is true; one cannot argue against a theory from a non-significant result.’

I felt most readers would be interested to read about tests of equivalence and Bayesian approaches, but many would be unfamiliar with these and might like to see an example of how they work in practice – if space permitted.

• Confidence intervals: I simply could not understand the first sentence – I wondered what was meant by ‘builds’ here. I understand about difficulties in comparing CI across studies when sample sizes differ, but I did not find the last sentence on p 4 easy to understand.
• P 5: The sentence starting: ‘The alpha value has the same interpretation’ was also hard to understand, especially the term ‘1-alpha CI’. Here too I felt some concrete illustration might be helpful to the reader. And again, I also found the reference to Bayesian intervals tantalising – I think many readers won’t know how to compute these and something like a figure comparing a traditional CI with a Bayesian interval and giving a source for those who want to read on would be very helpful. The reference to ‘credible intervals’ in the penultimate paragraph is very unclear and needs a supporting reference – most readers will not be familiar with this concept.

P 3, col 1, para 2, line 2; “allows us to compute”

P 3, col 2, para 2, ‘probability of replicating’

P 3, col 2, para 2, line 4 ‘informative about’

P 3, col 2, para 4, line 2 delete ‘of’

P 3, col 2, para 5, line 9 – ‘conditioned’ is either wrong or too technical here: would ‘based’ be acceptable as alternative wording

P 3, col 2, para 5, line 13 ‘This dichotomisation allows one to distinguish’

P 3, col 2, para 5, last sentence, delete ‘Alternatively’.

P 3, col 2, last para line 2 ‘first’

P 4, col 2, para 2, last sentence is hard to understand; not sure if this is better: ‘If sample sizes differ between studies, the distribution of CIs cannot be specified a priori’

P 5, col 1, para 2, ‘a pattern of order’ – I did not understand what was meant by this

P 5, col 1, para 2, last sentence unclear: possible rewording: “If the goal is to test the size of an effect then NHST is not the method of choice, since testing can only reject the null hypothesis.’ (??)

P 5, col 1, para 3, line 1 delete ‘that’

P 5, col 1, para 3, line 3 ‘on’ -> ‘by’

P 5, col 2, para 1, line 4 , rather than ‘Here I propose to adopt’ I suggest ‘I recommend adopting’

P 5, col 2, para 1, line 13 ‘with’ -> ‘by’

P 5, col 2, para 1 – recommend deleting last sentence

P 5, col 2, para 2, line 2 ‘consider’ -> ‘anticipate’

P 5, col 2, para 2, delete ‘should always be included’

P 5, col 2, para 2, ‘type one’ -> ‘Type I’

I have read this submission. I believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard, however I have significant reservations, as outlined above.

The University of Edinburgh, UK

I wondered about changing the focus slightly and modifying the title to reflect this to say something like: Null hypothesis significance testing: a guide to commonly misunderstood concepts and recommendations for good practice

Thank you for the suggestion – you indeed saw the intention behind the ‘tutorial’ style of the paper.

• P 3, col 1, para 3, last sentence. Although statisticians always emphasise the arbitrary nature of p < .05, we all know that in practice authors who use other values are likely to have their analyses queried. I wondered whether it would be useful here to note that in some disciplines different cutoffs are traditional, e.g. particle physics. Or you could cite David Colquhoun’s paper in which he recommends using p < .001 ( http://rsos.royalsocietypublishing.org/content/1/3/140216)  - just to be clear that the traditional p < .05 has been challenged.

I have added a sentence on this citing Colquhoun 2014 and the new Benjamin 2017 on using .005.

I agree that this point is always hard to appreciate, especially because it seems like in practice it makes little difference. I added a paragraph but using reaction times rather than a coin toss – thanks for the suggestion.

Added an example based on new table 1, following figure 1 – giving CI, equivalence tests and Bayes Factor (with refs to easy to use tools)

Changed builds to constructs (this simply means they are something we build) and added that the implication that probability coverage is not warranty when sample size change, is that we cannot compare CI.

I changed ‘ i.e. we accept that 1-alpha CI are wrong in alpha percent of the times in the long run’ to ‘, ‘e.g. a 95% CI is wrong in 5% of the times in the long run (i.e. if we repeat the experiment many times).’ – for Bayesian intervals I simply re-cited Morey & Rouder, 2011.

It is not the CI cannot be specified, it’s that the interval is not predictive of anything anymore! I changed it to ‘If sample sizes, however, differ between studies, there is no warranty that a CI from one study will be true at the rate alpha in a different study, which implies that CI cannot be compared across studies at this is rarely the same sample sizes’

I added (i.e. establish that A > B) – we test that conditions are ordered, but without further specification of the probability of that effect nor its size

Yes it works – thx

P 5, col 2, para 2, ‘type one’ -> ‘Type I’ 

Typos fixed, and suggestions accepted – thanks for that.

Stephen J. Senn

1 Luxembourg Institute of Health, Strassen, L-1445, Luxembourg

The revisions are OK for me, and I have changed my status to Approved.

I have read this submission. I believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard.

Referee response for version 2

On the whole I think that this article is reasonable, my main reservation being that I have my doubts on whether the literature needs yet another tutorial on this subject.

A further reservation I have is that the author, following others, stresses what in my mind is a relatively unimportant distinction between the Fisherian and Neyman-Pearson (NP) approaches. The distinction stressed by many is that the NP approach leads to a dichotomy accept/reject based on probabilities established in advance, whereas the Fisherian approach uses tail area probabilities calculated from the observed statistic. I see this as being unimportant and not even true. Unless one considers that the person carrying out a hypothesis test (original tester) is mandated to come to a conclusion on behalf of all scientific posterity, then one must accept that any remote scientist can come to his or her conclusion depending on the personal type I error favoured. To operate the results of an NP test carried out by the original tester, the remote scientist then needs to know the p-value. The type I error rate is then compared to this to come to a personal accept or reject decision (1). In fact Lehmann (2), who was an important developer of and proponent of the NP system, describes exactly this approach as being good practice. (See Testing Statistical Hypotheses, 2nd edition P70). Thus using tail-area probabilities calculated from the observed statistics does not constitute an operational difference between the two systems.

A more important distinction between the Fisherian and NP systems is that the former does not use alternative hypotheses(3). Fisher's opinion was that the null hypothesis was more primitive than the test statistic but that the test statistic was more primitive than the alternative hypothesis. Thus, alternative hypotheses could not be used to justify choice of test statistic. Only experience could do that.

Further distinctions between the NP and Fisherian approach are to do with conditioning and whether a null hypothesis can ever be accepted.

I have one minor quibble about terminology. As far as I can see, the author uses the usual term 'null hypothesis' and the eccentric term 'nil hypothesis' interchangeably. It would be simpler if the latter were abandoned.

Referee response for version 1

Marcel alm van assen.

1 Department of Methodology and Statistics, Tilburgh University, Tilburg, Netherlands

Null hypothesis significance testing (NHST) is a difficult topic, with misunderstandings arising easily. Many texts, including basic statistics books, deal with the topic, and attempt to explain it to students and anyone else interested. I would refer to a good basic text book, for a detailed explanation of NHST, or to a specialized article when wishing an explaining the background of NHST. So, what is the added value of a new text on NHST? In any case, the added value should be described at the start of this text. Moreover, the topic is so delicate and difficult that errors, misinterpretations, and disagreements are easy. I attempted to show this by giving comments to many sentences in the text.

Abstract: “null hypothesis significance testing is the statistical method of choice in biological, biomedical and social sciences to investigate if an effect is likely”. No, NHST is the method to test the hypothesis of no effect.

Intro: “Null hypothesis significance testing (NHST) is a method of statistical inference by which an observation is tested against a hypothesis of no effect or no relationship.” What is an ‘observation’? NHST is difficult to describe in one sentence, particularly here. I would skip this sentence entirely, here.

Section on Fisher; also explain the one-tailed test.

Section on Fisher; p(Obs|H0) does not reflect the verbal definition (the ‘or more extreme’ part).

Section on Fisher; use a reference and citation to Fisher’s interpretation of the p-value

Section on Fisher; “This was however only intended to be used as an indication that there is something in the data that deserves further investigation. The reason for this is that only H0 is tested whilst the effect under study is not itself being investigated.” First sentence, can you give a reference? Many people say a lot about Fisher’s intentions, but the good man is dead and cannot reply… Second sentence is a bit awkward, because the effect is investigated in a way, by testing the H0.

Section on p-value; Layout and structure can be improved greatly, by first again stating what the p-value is, and then statement by statement, what it is not, using separate lines for each statement. Consider adding that the p-value is randomly distributed under H0 (if all the assumptions of the test are met), and that under H1 the p-value is a function of population effect size and N; the larger each is, the smaller the p-value generally is.

Skip the sentence “If there is no effect, we should replicate the absence of effect with a probability equal to 1-p”. Not insightful, and you did not discuss the concept ‘replicate’ (and do not need to).

Skip the sentence “The total probability of false positives can also be obtained by aggregating results ( Ioannidis, 2005 ).” Not strongly related to p-values, and introduces unnecessary concepts ‘false positives’ (perhaps later useful) and ‘aggregation’.

Consider deleting; “If there is an effect however, the probability to replicate is a function of the (unknown) population effect size with no good way to know this from a single experiment ( Killeen, 2005 ).”

The following sentence; “ Finally, a (small) p-value  is not an indication favouring a hypothesis . A low p-value indicates a misfit of the null hypothesis to the data and cannot be taken as evidence in favour of a specific alternative hypothesis more than any other possible alternatives such as measurement error and selection bias ( Gelman, 2013 ).” is surely not mainstream thinking about NHST; I would surely delete that sentence. In NHST, a p-value is used for testing the H0. Why did you not yet discuss significance level? Yes, before discussing what is not a p-value, I would explain NHST (i.e., what it is and how it is used). 

Also the next sentence “The more (a priori) implausible the alternative hypothesis, the greater the chance that a finding is a false alarm ( Krzywinski & Altman, 2013 ;  Nuzzo, 2014 ).“ is not fully clear to me. This is a Bayesian statement. In NHST, no likelihoods are attributed to hypotheses; the reasoning is “IF H0 is true, then…”.

Last sentence: “As  Nickerson (2000)  puts it ‘theory corroboration requires the testing of multiple predictions because the chance of getting statistically significant results for the wrong reasons in any given case is high’.” What is relation of this sentence to the contents of this section, precisely?

Next section: “For instance, we can estimate that the probability of a given F value to be in the critical interval [+2 +∞] is less than 5%” This depends on the degrees of freedom.

“When there is no effect (H0 is true), the erroneous rejection of H0 is known as type I error and is equal to the p-value.” Strange sentence. The Type I error is the probability of erroneously rejecting the H0 (so, when it is true). The p-value is … well, you explained it before; it surely does not equal the Type I error.

Consider adding a figure explaining the distinction between Fisher’s logic and that of Neyman and Pearson.

“When the test statistics falls outside the critical region(s)” What is outside?

“There is a profound difference between accepting the null hypothesis and simply failing to reject it ( Killeen, 2005 )” I agree with you, but perhaps you may add that some statisticians simply define “accept H0’” as obtaining a p-value larger than the significance level. Did you already discuss the significance level, and it’s mostly used values?

“To accept or reject equally the null hypothesis, Bayesian approaches ( Dienes, 2014 ;  Kruschke, 2011 ) or confidence intervals must be used.” Is ‘reject equally’ appropriate English? Also using Cis, one cannot accept the H0.

Do you start discussing alpha only in the context of Cis?

“CI also indicates the precision of the estimate of effect size, but unless using a percentile bootstrap approach, they require assumptions about distributions which can lead to serious biases in particular regarding the symmetry and width of the intervals ( Wilcox, 2012 ).” Too difficult, using new concepts. Consider deleting.

“Assuming the CI (a)symmetry and width are correct, this gives some indication about the likelihood that a similar value can be observed in future studies, with 95% CI giving about 83% chance of replication success ( Lakens & Evers, 2014 ).” This statement is, in general, completely false. It very much depends on the sample sizes of both studies. If the replication study has a much, much, much larger N, then the probability that the original CI will contain the effect size of the replication approaches (1-alpha)*100%. If the original study has a much, much, much larger N, then the probability that the original Ci will contain the effect size of the replication study approaches 0%.

“Finally, contrary to p-values, CI can be used to accept H0. Typically, if a CI includes 0, we cannot reject H0. If a critical null region is specified rather than a single point estimate, for instance [-2 +2] and the CI is included within the critical null region, then H0 can be accepted. Importantly, the critical region must be specified a priori and cannot be determined from the data themselves.” No. H0 cannot be accepted with Cis.

“The (posterior) probability of an effect can however not be obtained using a frequentist framework.” Frequentist framework? You did not discuss that, yet.

“X% of times the CI obtained will contain the same parameter value”. The same? True, you mean?

“e.g. X% of the times the CI contains the same mean” I do not understand; which mean?

“The alpha value has the same interpretation as when using H0, i.e. we accept that 1-alpha CI are wrong in alpha percent of the times. “ What do you mean, CI are wrong? Consider rephrasing.

“To make a statement about the probability of a parameter of interest, likelihood intervals (maximum likelihood) and credibility intervals (Bayes) are better suited.” ML gives the likelihood of the data given the parameter, not the other way around.

“Many of the disagreements are not on the method itself but on its use.” Bayesians may disagree.

“If the goal is to establish the likelihood of an effect and/or establish a pattern of order, because both requires ruling out equivalence, then NHST is a good tool ( Frick, 1996 )” NHST does not provide evidence on the likelihood of an effect.

“If the goal is to establish some quantitative values, then NHST is not the method of choice.” P-values are also quantitative… this is not a precise sentence. And NHST may be used in combination with effect size estimation (this is even recommended by, e.g., the American Psychological Association (APA)).

“Because results are conditioned on H0, NHST cannot be used to establish beliefs.” It can reinforce some beliefs, e.g., if H0 or any other hypothesis, is true.

“To estimate the probability of a hypothesis, a Bayesian analysis is a better alternative.” It is the only alternative?

“Note however that even when a specific quantitative prediction from a hypothesis is shown to be true (typically testing H1 using Bayes), it does not prove the hypothesis itself, it only adds to its plausibility.” How can we show something is true?

I do not agree on the contents of the last section on ‘minimal reporting’. I prefer ‘optimal reporting’ instead, i.e., the reporting the information that is essential to the interpretation of the result, to any ready, which may have other goals than the writer of the article. This reporting includes, for sure, an estimate of effect size, and preferably a confidence interval, which is in line with recommendations of the APA.

I have read this submission. I believe that I have an appropriate level of expertise to state that I do not consider it to be of an acceptable scientific standard, for reasons outlined above.

The idea of this short review was to point to common interpretation errors (stressing again and again that we are under H0) being in using p-values or CI, and also proposing reporting practices to avoid bias. This is now stated at the end of abstract.

Regarding text books, it is clear that many fail to clearly distinguish Fisher/Pearson/NHST, see Glinet et al (2012) J. Exp Education 71, 83-92. If you have 1 or 2 in mind that you know to be good, I’m happy to include them.

I agree – yet people use it to investigate (not test) if an effect is likely. The issue here is wording. What about adding this distinction at the end of the sentence?: ‘null hypothesis significance testing is the statistical method of choice in biological, biomedical and social sciences used to investigate if an effect is likely, even though it actually tests for the hypothesis of no effect’.

I think a definition is needed, as it offers a starting point. What about the following: ‘NHST is a method of statistical inference by which an experimental factor is tested against a hypothesis of no effect or no relationship based on a given observation’

The section on Fisher has been modified (more or less) as suggested: (1) avoiding talking about one or two tailed tests (2) updating for p(Obs≥t|H0) and (3) referring to Fisher more explicitly (ie pages from articles and book) ; I cannot tell his intentions but these quotes leave little space to alternative interpretations.

The reasoning here is as you state yourself, part 1: ‘a p-value is used for testing the H0; and part 2: ‘no likelihoods are attributed to hypotheses’ it follows we cannot favour a hypothesis. It might seems contentious but this is the case that all we can is to reject the null – how could we favour a specific alternative hypothesis from there? This is explored further down the manuscript (and I now point to that) – note that we do not need to be Bayesian to favour a specific H1, all I’m saying is this cannot be attained with a p-value.

The point was to emphasise that a p value is not there to tell us a given H1 is true and can only be achieved through multiple predictions and experiments. I deleted it for clarity.

This sentence has been removed

Indeed, you are right and I have modified the text accordingly. When there is no effect (H0 is true), the erroneous rejection of H0 is known as type 1 error. Importantly, the type 1 error rate, or alpha value is determined a priori. It is a common mistake but the level of significance (for a given sample) is not the same as the frequency of acceptance alpha found on repeated sampling (Fisher, 1955).

A figure is now presented – with levels of acceptance, critical region, level of significance and p-value.

I should have clarified further here – as I was having in mind tests of equivalence. To clarify, I simply states now: ‘To accept the null hypothesis, tests of equivalence or Bayesian approaches must be used.’

It is now presented in the paragraph before.

Yes, you are right, I completely overlooked this problem. The corrected sentence (with more accurate ref) is now “Assuming the CI (a)symmetry and width are correct, this gives some indication about the likelihood that a similar value can be observed in future studies. For future studies of the same sample size, 95% CI giving about 83% chance of replication success (Cumming and Mallardet, 2006). If sample sizes differ between studies, CI do not however warranty any a priori coverage”.

Again, I had in mind equivalence testing, but in both cases you are right we can only reject and I therefore removed that sentence.

Yes, p-values must be interpreted in context with effect size, but this is not what people do. The point here is to be pragmatic, does and don’t. The sentence was changed.

Not for testing, but for probability, I am not aware of anything else.

Cumulative evidence is, in my opinion, the only way to show it. Even in hard science like physics multiple experiments. In the recent CERN study on finding Higgs bosons, 2 different and complementary experiments ran in parallel – and the cumulative evidence was taken as a proof of the true existence of Higgs bosons.

Daniel Lakens

1 School of Innovation Sciences, Eindhoven University of Technology, Eindhoven, Netherlands

I appreciate the author's attempt to write a short tutorial on NHST. Many people don't know how to use it, so attempts to educate people are always worthwhile. However, I don't think the current article reaches it's aim. For one, I think it might be practically impossible to explain a lot in such an ultra short paper - every section would require more than 2 pages to explain, and there are many sections. Furthermore, there are some excellent overviews, which, although more extensive, are also much clearer (e.g., Nickerson, 2000 ). Finally, I found many statements to be unclear, and perhaps even incorrect (noted below). Because there is nothing worse than creating more confusion on such a topic, I have extremely high standards before I think such a short primer should be indexed. I note some examples of unclear or incorrect statements below. I'm sorry I can't make a more positive recommendation.

“investigate if an effect is likely” – ambiguous statement. I think you mean, whether the observed DATA is probable, assuming there is no effect?

The Fisher (1959) reference is not correct – Fischer developed his method much earlier.

“This p-value thus reflects the conditional probability of achieving the observed outcome or larger, p(Obs|H0)” – please add 'assuming the null-hypothesis is true'.

“p(Obs|H0)” – explain this notation for novices.

“Following Fisher, the smaller the p-value, the greater the likelihood that the null hypothesis is false.”  This is wrong, and any statement about this needs to be much more precise. I would suggest direct quotes.

“there is something in the data that deserves further investigation” –unclear sentence.

“The reason for this” – unclear what ‘this’ refers to.

“ not the probability of the null hypothesis of being true, p(H0)” – second of can be removed?

“Any interpretation of the p-value in relation to the effect under study (strength, reliability, probability) is indeed

wrong, since the p-value is conditioned on H0”  - incorrect. A big problem is that it depends on the sample size, and that the probability of a theory depends on the prior.

“If there is no effect, we should replicate the absence of effect with a probability equal to 1-p.” I don’t understand this, but I think it is incorrect.

“The total probability of false positives can also be obtained by aggregating results (Ioannidis, 2005).” Unclear, and probably incorrect.

“By failing to reject, we simply continue to assume that H0 is true, which implies that one cannot, from a nonsignificant result, argue against a theory” – according to which theory? From a NP perspective, you can ACT as if the theory is false.

“(Lakens & Evers, 2014”) – we are not the original source, which should be cited instead.

“ Typically, if a CI includes 0, we cannot reject H0.”  - when would this not be the case? This assumes a CI of 1-alpha.

“If a critical null region is specified rather than a single point estimate, for instance [-2 +2] and the CI is included within the critical null region, then H0 can be accepted.” – you mean practically, or formally? I’m pretty sure only the former.

The section on ‘The (correct) use of NHST’ seems to conclude only Bayesian statistics should be used. I don’t really agree.

“ we can always argue that effect size, power, etc. must be reported.” – which power? Post-hoc power? Surely not? Other types are unknown. So what do you mean?

The recommendation on what to report remains vague, and it is unclear why what should be reported.

This sentence was changed, following as well the other reviewer, to ‘null hypothesis significance testing is the statistical method of choice in biological, biomedical and social sciences to investigate if an effect is likely, even though it actually tests whether the observed data are probable, assuming there is no effect’

Changed, refers to Fisher 1925

I changed a little the sentence structure, which should make explicit that this is the condition probability.

This has been changed to ‘[…] to decide whether the evidence is worth additional investigation and/or replication (Fisher, 1971 p13)’

my mistake – the sentence structure is now ‘ not the probability of the null hypothesis p(H0), of being true,’ ; hope this makes more sense (and this way refers back to p(Obs>t|H0)

Fair enough – my point was to stress the fact that p value and effect size or H1 have very little in common, but yes that the part in common has to do with sample size. I left the conditioning on H0 but also point out the dependency on sample size.

The whole paragraph was changed to reflect a more philosophical take on scientific induction/reasoning. I hope this is clearer.

Changed to refer to equivalence testing

I rewrote this, as to show frequentist analysis can be used  - I’m trying to sell Bayes more than any other approach.

I’m arguing we should report it all, that’s why there is no exhausting list – I can if needed.

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13.1 Understanding Null Hypothesis Testing

Learning objectives.

• Explain the purpose of null hypothesis testing, including the role of sampling error.
• Describe the basic logic of null hypothesis testing.
• Describe the role of relationship strength and sample size in determining statistical significance and make reasonable judgments about statistical significance based on these two factors.

The Purpose of Null Hypothesis Testing

As we have seen, psychological research typically involves measuring one or more variables for a sample and computing descriptive statistics for that sample. In general, however, the researcher’s goal is not to draw conclusions about that sample but to draw conclusions about the population that the sample was selected from. Thus researchers must use sample statistics to draw conclusions about the corresponding values in the population. These corresponding values in the population are called parameters . Imagine, for example, that a researcher measures the number of depressive symptoms exhibited by each of 50 clinically depressed adults and computes the mean number of symptoms. The researcher probably wants to use this sample statistic (the mean number of symptoms for the sample) to draw conclusions about the corresponding population parameter (the mean number of symptoms for clinically depressed adults).

Unfortunately, sample statistics are not perfect estimates of their corresponding population parameters. This is because there is a certain amount of random variability in any statistic from sample to sample. The mean number of depressive symptoms might be 8.73 in one sample of clinically depressed adults, 6.45 in a second sample, and 9.44 in a third—even though these samples are selected randomly from the same population. Similarly, the correlation (Pearson’s r ) between two variables might be +.24 in one sample, −.04 in a second sample, and +.15 in a third—again, even though these samples are selected randomly from the same population. This random variability in a statistic from sample to sample is called sampling error . (Note that the term error here refers to random variability and does not imply that anyone has made a mistake. No one “commits a sampling error.”)

One implication of this is that when there is a statistical relationship in a sample, it is not always clear that there is a statistical relationship in the population. A small difference between two group means in a sample might indicate that there is a small difference between the two group means in the population. But it could also be that there is no difference between the means in the population and that the difference in the sample is just a matter of sampling error. Similarly, a Pearson’s r value of −.29 in a sample might mean that there is a negative relationship in the population. But it could also be that there is no relationship in the population and that the relationship in the sample is just a matter of sampling error.

In fact, any statistical relationship in a sample can be interpreted in two ways:

• There is a relationship in the population, and the relationship in the sample reflects this.
• There is no relationship in the population, and the relationship in the sample reflects only sampling error.

The purpose of null hypothesis testing is simply to help researchers decide between these two interpretations.

The Logic of Null Hypothesis Testing

Null hypothesis testing is a formal approach to deciding between two interpretations of a statistical relationship in a sample. One interpretation is called the null hypothesis (often symbolized H 0 and read as “H-naught”). This is the idea that there is no relationship in the population and that the relationship in the sample reflects only sampling error. Informally, the null hypothesis is that the sample relationship “occurred by chance.” The other interpretation is called the alternative hypothesis (often symbolized as H 1 ). This is the idea that there is a relationship in the population and that the relationship in the sample reflects this relationship in the population.

Again, every statistical relationship in a sample can be interpreted in either of these two ways: It might have occurred by chance, or it might reflect a relationship in the population. So researchers need a way to decide between them. Although there are many specific null hypothesis testing techniques, they are all based on the same general logic. The steps are as follows:

• Assume for the moment that the null hypothesis is true. There is no relationship between the variables in the population.
• Determine how likely the sample relationship would be if the null hypothesis were true.
• If the sample relationship would be extremely unlikely, then reject the null hypothesis in favor of the alternative hypothesis. If it would not be extremely unlikely, then retain the null hypothesis .

Following this logic, we can begin to understand why Mehl and his colleagues concluded that there is no difference in talkativeness between women and men in the population. In essence, they asked the following question: “If there were no difference in the population, how likely is it that we would find a small difference of d = 0.06 in our sample?” Their answer to this question was that this sample relationship would be fairly likely if the null hypothesis were true. Therefore, they retained the null hypothesis—concluding that there is no evidence of a sex difference in the population. We can also see why Kanner and his colleagues concluded that there is a correlation between hassles and symptoms in the population. They asked, “If the null hypothesis were true, how likely is it that we would find a strong correlation of +.60 in our sample?” Their answer to this question was that this sample relationship would be fairly unlikely if the null hypothesis were true. Therefore, they rejected the null hypothesis in favor of the alternative hypothesis—concluding that there is a positive correlation between these variables in the population.

A crucial step in null hypothesis testing is finding the likelihood of the sample result if the null hypothesis were true. This probability is called the p value . A low p value means that the sample result would be unlikely if the null hypothesis were true and leads to the rejection of the null hypothesis. A high p value means that the sample result would be likely if the null hypothesis were true and leads to the retention of the null hypothesis. But how low must the p value be before the sample result is considered unlikely enough to reject the null hypothesis? In null hypothesis testing, this criterion is called α (alpha) and is almost always set to .05. If there is less than a 5% chance of a result as extreme as the sample result if the null hypothesis were true, then the null hypothesis is rejected. When this happens, the result is said to be statistically significant . If there is greater than a 5% chance of a result as extreme as the sample result when the null hypothesis is true, then the null hypothesis is retained. This does not necessarily mean that the researcher accepts the null hypothesis as true—only that there is not currently enough evidence to conclude that it is true. Researchers often use the expression “fail to reject the null hypothesis” rather than “retain the null hypothesis,” but they never use the expression “accept the null hypothesis.”

The Misunderstood p Value

The p value is one of the most misunderstood quantities in psychological research (Cohen, 1994). Even professional researchers misinterpret it, and it is not unusual for such misinterpretations to appear in statistics textbooks!

The most common misinterpretation is that the p value is the probability that the null hypothesis is true—that the sample result occurred by chance. For example, a misguided researcher might say that because the p value is .02, there is only a 2% chance that the result is due to chance and a 98% chance that it reflects a real relationship in the population. But this is incorrect . The p value is really the probability of a result at least as extreme as the sample result if the null hypothesis were true. So a p value of .02 means that if the null hypothesis were true, a sample result this extreme would occur only 2% of the time.

You can avoid this misunderstanding by remembering that the p value is not the probability that any particular hypothesis is true or false. Instead, it is the probability of obtaining the sample result if the null hypothesis were true.

Role of Sample Size and Relationship Strength

Recall that null hypothesis testing involves answering the question, “If the null hypothesis were true, what is the probability of a sample result as extreme as this one?” In other words, “What is the p value?” It can be helpful to see that the answer to this question depends on just two considerations: the strength of the relationship and the size of the sample. Specifically, the stronger the sample relationship and the larger the sample, the less likely the result would be if the null hypothesis were true. That is, the lower the p value. This should make sense. Imagine a study in which a sample of 500 women is compared with a sample of 500 men in terms of some psychological characteristic, and Cohen’s d is a strong 0.50. If there were really no sex difference in the population, then a result this strong based on such a large sample should seem highly unlikely. Now imagine a similar study in which a sample of three women is compared with a sample of three men, and Cohen’s d is a weak 0.10. If there were no sex difference in the population, then a relationship this weak based on such a small sample should seem likely. And this is precisely why the null hypothesis would be rejected in the first example and retained in the second.

Of course, sometimes the result can be weak and the sample large, or the result can be strong and the sample small. In these cases, the two considerations trade off against each other so that a weak result can be statistically significant if the sample is large enough and a strong relationship can be statistically significant even if the sample is small. Table 13.1 “How Relationship Strength and Sample Size Combine to Determine Whether a Result Is Statistically Significant” shows roughly how relationship strength and sample size combine to determine whether a sample result is statistically significant. The columns of the table represent the three levels of relationship strength: weak, medium, and strong. The rows represent four sample sizes that can be considered small, medium, large, and extra large in the context of psychological research. Thus each cell in the table represents a combination of relationship strength and sample size. If a cell contains the word Yes , then this combination would be statistically significant for both Cohen’s d and Pearson’s r . If it contains the word No , then it would not be statistically significant for either. There is one cell where the decision for d and r would be different and another where it might be different depending on some additional considerations, which are discussed in Section 13.2 “Some Basic Null Hypothesis Tests”

Table 13.1 How Relationship Strength and Sample Size Combine to Determine Whether a Result Is Statistically Significant

Although Table 13.1 “How Relationship Strength and Sample Size Combine to Determine Whether a Result Is Statistically Significant” provides only a rough guideline, it shows very clearly that weak relationships based on medium or small samples are never statistically significant and that strong relationships based on medium or larger samples are always statistically significant. If you keep this in mind, you will often know whether a result is statistically significant based on the descriptive statistics alone. It is extremely useful to be able to develop this kind of intuitive judgment. One reason is that it allows you to develop expectations about how your formal null hypothesis tests are going to come out, which in turn allows you to detect problems in your analyses. For example, if your sample relationship is strong and your sample is medium, then you would expect to reject the null hypothesis. If for some reason your formal null hypothesis test indicates otherwise, then you need to double-check your computations and interpretations. A second reason is that the ability to make this kind of intuitive judgment is an indication that you understand the basic logic of this approach in addition to being able to do the computations.

Statistical Significance Versus Practical Significance

Table 13.1 “How Relationship Strength and Sample Size Combine to Determine Whether a Result Is Statistically Significant” illustrates another extremely important point. A statistically significant result is not necessarily a strong one. Even a very weak result can be statistically significant if it is based on a large enough sample. This is closely related to Janet Shibley Hyde’s argument about sex differences (Hyde, 2007). The differences between women and men in mathematical problem solving and leadership ability are statistically significant. But the word significant can cause people to interpret these differences as strong and important—perhaps even important enough to influence the college courses they take or even who they vote for. As we have seen, however, these statistically significant differences are actually quite weak—perhaps even “trivial.”

This is why it is important to distinguish between the statistical significance of a result and the practical significance of that result. Practical significance refers to the importance or usefulness of the result in some real-world context. Many sex differences are statistically significant—and may even be interesting for purely scientific reasons—but they are not practically significant. In clinical practice, this same concept is often referred to as “clinical significance.” For example, a study on a new treatment for social phobia might show that it produces a statistically significant positive effect. Yet this effect still might not be strong enough to justify the time, effort, and other costs of putting it into practice—especially if easier and cheaper treatments that work almost as well already exist. Although statistically significant, this result would be said to lack practical or clinical significance.

Key Takeaways

• Null hypothesis testing is a formal approach to deciding whether a statistical relationship in a sample reflects a real relationship in the population or is just due to chance.
• The logic of null hypothesis testing involves assuming that the null hypothesis is true, finding how likely the sample result would be if this assumption were correct, and then making a decision. If the sample result would be unlikely if the null hypothesis were true, then it is rejected in favor of the alternative hypothesis. If it would not be unlikely, then the null hypothesis is retained.
• The probability of obtaining the sample result if the null hypothesis were true (the p value) is based on two considerations: relationship strength and sample size. Reasonable judgments about whether a sample relationship is statistically significant can often be made by quickly considering these two factors.
• Statistical significance is not the same as relationship strength or importance. Even weak relationships can be statistically significant if the sample size is large enough. It is important to consider relationship strength and the practical significance of a result in addition to its statistical significance.
• Discussion: Imagine a study showing that people who eat more broccoli tend to be happier. Explain for someone who knows nothing about statistics why the researchers would conduct a null hypothesis test.

Practice: Use Table 13.1 “How Relationship Strength and Sample Size Combine to Determine Whether a Result Is Statistically Significant” to decide whether each of the following results is statistically significant.

• The correlation between two variables is r = −.78 based on a sample size of 137.
• The mean score on a psychological characteristic for women is 25 ( SD = 5) and the mean score for men is 24 ( SD = 5). There were 12 women and 10 men in this study.
• In a memory experiment, the mean number of items recalled by the 40 participants in Condition A was 0.50 standard deviations greater than the mean number recalled by the 40 participants in Condition B.
• In another memory experiment, the mean scores for participants in Condition A and Condition B came out exactly the same!
• A student finds a correlation of r = .04 between the number of units the students in his research methods class are taking and the students’ level of stress.

Cohen, J. (1994). The world is round: p < .05. American Psychologist, 49 , 997–1003.

Hyde, J. S. (2007). New directions in the study of gender similarities and differences. Current Directions in Psychological Science , 16 , 259–263.

Research Methods in Psychology Copyright © 2016 by University of Minnesota is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License , except where otherwise noted.

NULL HYPOTHESIS

the statement postulating an experiment will find no variations between the control and experimental states, which is, no union between variants. Statistical tests are rendered to experimental outcomes in effort to disprove or refute the previously established significance level .

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In this post

Before carrying out their research, most psychologists will make a prediction about what will happen. This is known as a hypothesis, which is a statement regarding what the psychologist believes will or should happen at the end of the study. For example, a psychologist may predict that children who listen to music whilst revising will do better in their exams than those children who do not.

There are two types of hypotheses, which are null hypotheses and alternative hypotheses, both of which we will look at now in more detail.

What is a null hypothesis?

A null hypothesis predicts that there will be no pattern or trend in results. In other words, it predicts no difference and no correlation . (A correlation is a relationship between two or more things.)

Before starting their research, psychologists usually have both a null and an alternative hypothesis and their aim is to find out which one is correct. Once they have identified which one is correct they will reject the other, as this one will not be supported by their research findings.

What is an alternative hypothesis?

Unlike a null hypothesis, an alternative hypothesis predicts that there will be a difference or a correlation between two or more things. In other words, an alternative hypothesis predicts some kind of pattern or trend in results. Have a look at the following alternative hypotheses, which are based around the core studies within this course:

• Participants will be able to accurately recall more information at the start and end of a list than in the middle
• Children whose efforts are praised are more likely to grow up with a growth mindset than those who are praised personally
• Children are more likely to behave aggressively when they witnessed an aggressive adult role model
• Children under the age of eight are more likely to be egocentric than those who are over the age of eight.

It will help you in the exam, if you are asked to write some form of hypothesis, if you begin a null hypothesis with “there will be no…” and an alternative hypothesis with “there will be a…”. There are usually two marks available for writing a hypothesis correctly. One mark will be for knowing whether it is predicting a different or a correlation or not and the other mark will be for stating the rest of the hypothesis, i.e. the variables, which must be done in a clear and accurate way.

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Understanding Null Hypothesis Testing

Rajiv S. Jhangiani; I-Chant A. Chiang; Carrie Cuttler; and Dana C. Leighton

Learning Objectives

• Explain the purpose of null hypothesis testing, including the role of sampling error.
• Describe the basic logic of null hypothesis testing.
• Describe the role of relationship strength and sample size in determining statistical significance and make reasonable judgments about statistical significance based on these two factors.

 The Purpose of Null Hypothesis Testing

As we have seen, psychological research typically involves measuring one or more variables in a sample and computing descriptive summary data (e.g., means, correlation coefficients) for those variables. These descriptive data for the sample are called statistics .  In general, however, the researcher’s goal is not to draw conclusions about that sample but to draw conclusions about the population that the sample was selected from. Thus researchers must use sample statistics to draw conclusions about the corresponding values in the population. These corresponding values in the population are called parameters . Imagine, for example, that a researcher measures the number of depressive symptoms exhibited by each of 50 adults with clinical depression and computes the mean number of symptoms. The researcher probably wants to use this sample statistic (the mean number of symptoms for the sample) to draw conclusions about the corresponding population parameter (the mean number of symptoms for adults with clinical depression).

Unfortunately, sample statistics are not perfect estimates of their corresponding population parameters. This is because there is a certain amount of random variability in any statistic from sample to sample. The mean number of depressive symptoms might be 8.73 in one sample of adults with clinical depression, 6.45 in a second sample, and 9.44 in a third—even though these samples are selected randomly from the same population. Similarly, the correlation (Pearson’s  r ) between two variables might be +.24 in one sample, −.04 in a second sample, and +.15 in a third—again, even though these samples are selected randomly from the same population. This random variability in a statistic from sample to sample is called  sampling error . (Note that the term error  here refers to random variability and does not imply that anyone has made a mistake. No one “commits a sampling error.”)

One implication of this is that when there is a statistical relationship in a sample, it is not always clear that there is a statistical relationship in the population. A small difference between two group means in a sample might indicate that there is a small difference between the two group means in the population. But it could also be that there is no difference between the means in the population and that the difference in the sample is just a matter of sampling error. Similarly, a Pearson’s  r  value of −.29 in a sample might mean that there is a negative relationship in the population. But it could also be that there is no relationship in the population and that the relationship in the sample is just a matter of sampling error.

In fact, any statistical relationship in a sample can be interpreted in two ways:

• There is a relationship in the population, and the relationship in the sample reflects this.
• There is no relationship in the population, and the relationship in the sample reflects only sampling error.

The purpose of null hypothesis testing is simply to help researchers decide between these two interpretations.

The Logic of Null Hypothesis Testing

Null hypothesis testing (often called null hypothesis significance testing or NHST) is a formal approach to deciding between two interpretations of a statistical relationship in a sample. One interpretation is called the   null hypothesis  (often symbolized  H 0 and read as “H-zero”). This is the idea that there is no relationship in the population and that the relationship in the sample reflects only sampling error. Informally, the null hypothesis is that the sample relationship “occurred by chance.” The other interpretation is called the alternative hypothesis  (often symbolized as  H 1 ). This is the idea that there is a relationship in the population and that the relationship in the sample reflects this relationship in the population.

Again, every statistical relationship in a sample can be interpreted in either of these two ways: It might have occurred by chance, or it might reflect a relationship in the population. So researchers need a way to decide between them. Although there are many specific null hypothesis testing techniques, they are all based on the same general logic. The steps are as follows:

• Assume for the moment that the null hypothesis is true. There is no relationship between the variables in the population.
• Determine how likely the sample relationship would be if the null hypothesis were true.
• If the sample relationship would be extremely unlikely, then reject the null hypothesis  in favor of the alternative hypothesis. If it would not be extremely unlikely, then  retain the null hypothesis .

Following this logic, we can begin to understand why Mehl and his colleagues concluded that there is no difference in talkativeness between women and men in the population. In essence, they asked the following question: “If there were no difference in the population, how likely is it that we would find a small difference of  d  = 0.06 in our sample?” Their answer to this question was that this sample relationship would be fairly likely if the null hypothesis were true. Therefore, they retained the null hypothesis—concluding that there is no evidence of a sex difference in the population. We can also see why Kanner and his colleagues concluded that there is a correlation between hassles and symptoms in the population. They asked, “If the null hypothesis were true, how likely is it that we would find a strong correlation of +.60 in our sample?” Their answer to this question was that this sample relationship would be fairly unlikely if the null hypothesis were true. Therefore, they rejected the null hypothesis in favor of the alternative hypothesis—concluding that there is a positive correlation between these variables in the population.

A crucial step in null hypothesis testing is finding the probability of the sample result or a more extreme result if the null hypothesis were true (Lakens, 2017). [1] This probability is called the p value . A low  p value means that the sample or more extreme result would be unlikely if the null hypothesis were true and leads to the rejection of the null hypothesis. A p value that is not low means that the sample or more extreme result would be likely if the null hypothesis were true and leads to the retention of the null hypothesis. But how low must the p value criterion be before the sample result is considered unlikely enough to reject the null hypothesis? In null hypothesis testing, this criterion is called α (alpha) and is almost always set to .05. If there is a 5% chance or less of a result at least as extreme as the sample result if the null hypothesis were true, then the null hypothesis is rejected. When this happens, the result is said to be statistically significant . If there is greater than a 5% chance of a result as extreme as the sample result when the null hypothesis is true, then the null hypothesis is retained. This does not necessarily mean that the researcher accepts the null hypothesis as true—only that there is not currently enough evidence to reject it. Researchers often use the expression “fail to reject the null hypothesis” rather than “retain the null hypothesis,” but they never use the expression “accept the null hypothesis.”

The Misunderstood  p  Value

The  p  value is one of the most misunderstood quantities in psychological research (Cohen, 1994) [2] . Even professional researchers misinterpret it, and it is not unusual for such misinterpretations to appear in statistics textbooks!

The most common misinterpretation is that the  p  value is the probability that the null hypothesis is true—that the sample result occurred by chance. For example, a misguided researcher might say that because the  p  value is .02, there is only a 2% chance that the result is due to chance and a 98% chance that it reflects a real relationship in the population. But this is incorrect . The  p  value is really the probability of a result at least as extreme as the sample result  if  the null hypothesis  were  true. So a  p  value of .02 means that if the null hypothesis were true, a sample result this extreme would occur only 2% of the time.

You can avoid this misunderstanding by remembering that the  p  value is not the probability that any particular  hypothesis  is true or false. Instead, it is the probability of obtaining the  sample result  if the null hypothesis were true.

Role of Sample Size and Relationship Strength

Recall that null hypothesis testing involves answering the question, “If the null hypothesis were true, what is the probability of a sample result as extreme as this one?” In other words, “What is the  p  value?” It can be helpful to see that the answer to this question depends on just two considerations: the strength of the relationship and the size of the sample. Specifically, the stronger the sample relationship and the larger the sample, the less likely the result would be if the null hypothesis were true. That is, the lower the  p  value. This should make sense. Imagine a study in which a sample of 500 women is compared with a sample of 500 men in terms of some psychological characteristic, and Cohen’s  d  is a strong 0.50. If there were really no sex difference in the population, then a result this strong based on such a large sample should seem highly unlikely. Now imagine a similar study in which a sample of three women is compared with a sample of three men, and Cohen’s  d  is a weak 0.10. If there were no sex difference in the population, then a relationship this weak based on such a small sample should seem likely. And this is precisely why the null hypothesis would be rejected in the first example and retained in the second.

Of course, sometimes the result can be weak and the sample large, or the result can be strong and the sample small. In these cases, the two considerations trade off against each other so that a weak result can be statistically significant if the sample is large enough and a strong relationship can be statistically significant even if the sample is small. Table 13.1 shows roughly how relationship strength and sample size combine to determine whether a sample result is statistically significant. The columns of the table represent the three levels of relationship strength: weak, medium, and strong. The rows represent four sample sizes that can be considered small, medium, large, and extra large in the context of psychological research. Thus each cell in the table represents a combination of relationship strength and sample size. If a cell contains the word  Yes , then this combination would be statistically significant for both Cohen’s  d  and Pearson’s  r . If it contains the word  No , then it would not be statistically significant for either. There is one cell where the decision for  d  and  r  would be different and another where it might be different depending on some additional considerations, which are discussed in Section 13.2 “Some Basic Null Hypothesis Tests”

Although Table 13.1 provides only a rough guideline, it shows very clearly that weak relationships based on medium or small samples are never statistically significant and that strong relationships based on medium or larger samples are always statistically significant. If you keep this lesson in mind, you will often know whether a result is statistically significant based on the descriptive statistics alone. It is extremely useful to be able to develop this kind of intuitive judgment. One reason is that it allows you to develop expectations about how your formal null hypothesis tests are going to come out, which in turn allows you to detect problems in your analyses. For example, if your sample relationship is strong and your sample is medium, then you would expect to reject the null hypothesis. If for some reason your formal null hypothesis test indicates otherwise, then you need to double-check your computations and interpretations. A second reason is that the ability to make this kind of intuitive judgment is an indication that you understand the basic logic of this approach in addition to being able to do the computations.

Statistical Significance Versus Practical Significance

Table 13.1 illustrates another extremely important point. A statistically significant result is not necessarily a strong one. Even a very weak result can be statistically significant if it is based on a large enough sample. This is closely related to Janet Shibley Hyde’s argument about sex differences (Hyde, 2007) [3] . The differences between women and men in mathematical problem solving and leadership ability are statistically significant. But the word  significant  can cause people to interpret these differences as strong and important—perhaps even important enough to influence the college courses they take or even who they vote for. As we have seen, however, these statistically significant differences are actually quite weak—perhaps even “trivial.”

This is why it is important to distinguish between the  statistical  significance of a result and the  practical  significance of that result.  Practical significance refers to the importance or usefulness of the result in some real-world context. Many sex differences are statistically significant—and may even be interesting for purely scientific reasons—but they are not practically significant. In clinical practice, this same concept is often referred to as “clinical significance.” For example, a study on a new treatment for social phobia might show that it produces a statistically significant positive effect. Yet this effect still might not be strong enough to justify the time, effort, and other costs of putting it into practice—especially if easier and cheaper treatments that work almost as well already exist. Although statistically significant, this result would be said to lack practical or clinical significance.

Image Description

“Null Hypothesis” long description:  A comic depicting a man and a woman talking in the foreground. In the background is a child working at a desk. The man says to the woman, “I can’t believe schools are still teaching kids about the null hypothesis. I remember reading a big study that conclusively disproved it  years  ago.”  [Return to “Null Hypothesis”]

“Conditional Risk” long description:  A comic depicting two hikers beside a tree during a thunderstorm. A bolt of lightning goes “crack” in the dark sky as thunder booms. One of the hikers says, “Whoa! We should get inside!” The other hiker says, “It’s okay! Lightning only kills about 45 Americans a year, so the chances of dying are only one in 7,000,000. Let’s go on!” The comic’s caption says, “The annual death rate among people who know that statistic is one in six.”  [Return to “Conditional Risk”]

Media Attributions

• Null Hypothesis  by XKCD  CC BY-NC (Attribution NonCommercial)
• Conditional Risk  by XKCD  CC BY-NC (Attribution NonCommercial)
• Lakens, D. (2017, December 25). About p -values: Understanding common misconceptions. [Blog post] Retrieved from https://correlaid.org/en/blog/understand-p-values/ ↵
• Cohen, J. (1994). The world is round: p < .05. American Psychologist, 49 , 997–1003. ↵
• Hyde, J. S. (2007). New directions in the study of gender similarities and differences. Current Directions in Psychological Science, 16 , 259–263. ↵

Descriptive data that involves measuring one or more variables in a sample and computing descriptive summary data (e.g., means, correlation coefficients) for those variables.

Corresponding values in the population.

The random variability in a statistic from sample to sample.

A formal approach to deciding between two interpretations of a statistical relationship in a sample.

The idea that there is no relationship in the population and that the relationship in the sample reflects only sampling error (often symbolized H0 and read as “H-zero”).

An alternative to the null hypothesis (often symbolized as H1), this hypothesis proposes that there is a relationship in the population and that the relationship in the sample reflects this relationship in the population.

A decision made by researchers using null hypothesis testing which occurs when the sample relationship would be extremely unlikely.

A decision made by researchers in null hypothesis testing which occurs when the sample relationship would not be extremely unlikely.

The probability of obtaining the sample result or a more extreme result if the null hypothesis were true.

The criterion that shows how low a p-value should be before the sample result is considered unlikely enough to reject the null hypothesis (Usually set to .05).

An effect that is unlikely due to random chance and therefore likely represents a real effect in the population.

Refers to the importance or usefulness of the result in some real-world context.

Understanding Null Hypothesis Testing Copyright © by Rajiv S. Jhangiani; I-Chant A. Chiang; Carrie Cuttler; and Dana C. Leighton is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License , except where otherwise noted.

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Unexpected expression and function of fcεri in immortalized breast cancer cells: a cautionary null study.

1. Introduction

2. materials and methods, 2.1. cell lines, 2.2. antibodies, 2.3. chloroacetate esterase staining (cae), 2.4. immunofluorescence, 2.5. flow cytometry, 2.5.1. fcεri flow cytometry, 2.5.2. ca 2+ flux assay, 2.6. polymerase chain reaction (pcr), 2.6.1. rna extraction, 2.6.2. cdna synthesis, 2.6.4. gel electrophoresis, 2.7. immunoblotting, 2.8. il-6 elisa, 2.9. r-2 genomic data search, 3.1. cae staining, 3.2. fluorescence, fcεri in 4t1 tumors in vivo, 3.3. anti-fcεriα expression in vitro, 3.4. ca 2+ flux assay, 3.6. il-6 elisa, 3.7. r2 genomics, 4. discussion, 5. conclusions, author contributions, institutional review board statement, informed consent statement, data availability statement, acknowledgments, conflicts of interest.

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Ashbaugh, A.M.; Lyons, D.O.; Keyser, C.M.; Pullen, N.A. Unexpected Expression and Function of FcεRI in Immortalized Breast Cancer Cells: A Cautionary Null Study. Cells 2024 , 13 , 1399. https://doi.org/10.3390/cells13161399

Ashbaugh AM, Lyons DO, Keyser CM, Pullen NA. Unexpected Expression and Function of FcεRI in Immortalized Breast Cancer Cells: A Cautionary Null Study. Cells . 2024; 13(16):1399. https://doi.org/10.3390/cells13161399

Ashbaugh, Alexandria M., David O. Lyons, Carianna M. Keyser, and Nicholas A. Pullen. 2024. "Unexpected Expression and Function of FcεRI in Immortalized Breast Cancer Cells: A Cautionary Null Study" Cells 13, no. 16: 1399. https://doi.org/10.3390/cells13161399

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Chapter 13: Inferential Statistics

13.2 some basic null hypothesis tests, learning objectives.

• Conduct and interpret one-sample, dependent-samples, and independent-samples t tests.
• Interpret the results of one-way, repeated measures, and factorial ANOVAs.
• Conduct and interpret null hypothesis tests of Pearson’s r .

In this section, we look at several common null hypothesis testing procedures. The emphasis here is on providing enough information to allow you to conduct and interpret the most basic versions. In most cases, the online statistical analysis tools mentioned in Chapter 12 “Descriptive Statistics” will handle the computations—as will programs such as Microsoft Excel and SPSS.

As we have seen throughout this book, many studies in psychology focus on the difference between two means. The most common null hypothesis test for this type of statistical relationship is the t test . In this section, we look at three types of t tests that are used for slightly different research designs: the one-sample t test, the dependent-samples t test, and the independent-samples t test.

One-Sample t Test

The one-sample t test is used to compare a sample mean ( M ) with a hypothetical population mean (μ 0 ) that provides some interesting standard of comparison. The null hypothesis is that the mean for the population (µ) is equal to the hypothetical population mean: μ = μ 0 . The alternative hypothesis is that the mean for the population is different from the hypothetical population mean: μ ≠ μ 0 . To decide between these two hypotheses, we need to find the probability of obtaining the sample mean (or one more extreme) if the null hypothesis were true. But finding this p value requires first computing a test statistic called t . (A test statistic is a statistic that is computed only to help find the p value.) The formula for t is as follows:

\[ t = \frac{M – \mu_{0}}{( \frac{SD}{ \sqrt{N}})} \]

Again, M is the sample mean and µ 0 is the hypothetical population mean of interest. SD is the sample standard deviation and N is the sample size.

The reason the t statistic (or any test statistic) is useful is that we know how it is distributed when the null hypothesis is true. As shown in Figure 13.1 “Distribution of “ , this distribution is unimodal and symmetrical, and it has a mean of 0. Its precise shape depends on a statistical concept called the degrees of freedom, which for a one-sample t test is N − 1. (There are 24 degrees of freedom for the distribution shown in Figure 13.1 “Distribution of “ .) The important point is that knowing this distribution makes it possible to find the p value for any t score. Consider, for example, a t score of +1.50 based on a sample of 25. The probability of a t score at least this extreme is given by the proportion of t scores in the distribution that are at least this extreme. For now, let us define extreme as being far from zero in either direction. Thus the p value is the proportion of t scores that are +1.50 or above or that are −1.50 or below—a value that turns out to be .14.

Figure 13.1 Distribution of t Scores (With 24 Degrees of Freedom) When the Null Hypothesis Is True

The red vertical lines represent the two-tailed critical values, and the green vertical lines the one-tailed critical values when α = .05.

Fortunately, we do not have to deal directly with the distribution of t scores. If we were to enter our sample data and hypothetical mean of interest into one of the online statistical tools in Chapter 12 “Descriptive Statistics” or into a program like SPSS (Excel does not have a one-sample t test function), the output would include both the t score and the p value. At this point, the rest of the procedure is simple. If p is less than .05, we reject the null hypothesis and conclude that the population mean differs from the hypothetical mean of interest. If p is greater than .05, we retain the null hypothesis and conclude that there is not enough evidence to say that the population mean differs from the hypothetical mean of interest. (Again, technically, we conclude only that we do not have enough evidence to conclude that it does differ.)

If we were to compute the t score by hand, we could use a table like Table 13.2 “Table of Critical Values of “ to make the decision. This table does not provide actual p values. Instead, it provides the critical values of t for different degrees of freedom ( df) when α is .05. For now, let us focus on the two-tailed critical values in the last column of the table. Each of these values should be interpreted as a pair of values: one positive and one negative. For example, the two-tailed critical values when there are 24 degrees of freedom are +2.064 and −2.064. These are represented by the red vertical lines in Figure 13.1 “Distribution of “ . The idea is that any t score below the lower critical value (the left-hand red line in Figure 13.1 “Distribution of “ ) is in the lowest 2.5% of the distribution, while any t score above the upper critical value (the right-hand red line) is in the highest 2.5% of the distribution. This means that any t score beyond the critical value in either direction is in the most extreme 5% of t scores when the null hypothesis is true and therefore has a p value less than .05. Thus if the t score we compute is beyond the critical value in either direction, then we reject the null hypothesis. If the t score we compute is between the upper and lower critical values, then we retain the null hypothesis.

Table 13.2 Table of Critical Values of t When α = .05

Thus far, we have considered what is called a two-tailed test , where we reject the null hypothesis if the t score for the sample is extreme in either direction. This makes sense when we believe that the sample mean might differ from the hypothetical population mean but we do not have good reason to expect the difference to go in a particular direction. But it is also possible to do a one-tailed test , where we reject the null hypothesis only if the t score for the sample is extreme in one direction that we specify before collecting the data. This makes sense when we have good reason to expect the sample mean will differ from the hypothetical population mean in a particular direction.

Here is how it works. Each one-tailed critical value in Table 13.2 “Table of Critical Values of “ can again be interpreted as a pair of values: one positive and one negative. A t score below the lower critical value is in the lowest 5% of the distribution, and a t score above the upper critical value is in the highest 5% of the distribution. For 24 degrees of freedom, these values are −1.711 and +1.711. (These are represented by the green vertical lines in Figure 13.1 “Distribution of “ .) However, for a one-tailed test, we must decide before collecting data whether we expect the sample mean to be lower than the hypothetical population mean, in which case we would use only the lower critical value, or we expect the sample mean to be greater than the hypothetical population mean, in which case we would use only the upper critical value. Notice that we still reject the null hypothesis when the t score for our sample is in the most extreme 5% of the t scores we would expect if the null hypothesis were true—so α remains at .05. We have simply redefined extreme to refer only to one tail of the distribution. The advantage of the one-tailed test is that critical values are less extreme. If the sample mean differs from the hypothetical population mean in the expected direction, then we have a better chance of rejecting the null hypothesis. The disadvantage is that if the sample mean differs from the hypothetical population mean in the unexpected direction, then there is no chance at all of rejecting the null hypothesis.

Example One-Sample t Test

Imagine that a health psychologist is interested in the accuracy of college students’ estimates of the number of calories in a chocolate chip cookie. He shows the cookie to a sample of 10 students and asks each one to estimate the number of calories in it. Because the actual number of calories in the cookie is 250, this is the hypothetical population mean of interest (µ 0 ). The null hypothesis is that the mean estimate for the population (μ) is 250. Because he has no real sense of whether the students will underestimate or overestimate the number of calories, he decides to do a two-tailed test. Now imagine further that the participants’ actual estimates are as follows:

250, 280, 200, 150, 175, 200, 200, 220, 180, 250.

The mean estimate for the sample ( M ) is 212.00 calories and the standard deviation ( SD ) is 39.17. The health psychologist can now compute the t score for his sample:

\[ t = \frac{212 – 250}{ ( \frac{39.17}{ \sqrt{10}} ) } = -3.07 \]

If he enters the data into one of the online analysis tools or uses SPSS, it would also tell him that the two-tailed p value for this t score (with 10 − 1 = 9 degrees of freedom) is .013. Because this is less than .05, the health psychologist would reject the null hypothesis and conclude that college students tend to underestimate the number of calories in a chocolate chip cookie. If he computes the t score by hand, he could look at Table 13.2 “Table of Critical Values of “ and see that the critical value of t for a two-tailed test with 9 degrees of freedom is ±2.262. The fact that his t score was more extreme than this critical value would tell him that his p value is less than .05 and that he should reject the null hypothesis.

Finally, if this researcher had gone into this study with good reason to expect that college students underestimate the number of calories, then he could have done a one-tailed test instead of a two-tailed test. The only thing this would change is the critical value, which would be −1.833. This slightly less extreme value would make it a bit easier to reject the null hypothesis. However, if it turned out that college students overestimate the number of calories—no matter how much they overestimate it—the researcher would not have been able to reject the null hypothesis.

The Dependent-Samples t Test

The dependent-samples t test (sometimes called the paired-samples t test) is used to compare two means for the same sample tested at two different times or under two different conditions. This makes it appropriate for pretest-posttest designs or within-subjects experiments. The null hypothesis is that the means at the two times or under the two conditions are the same in the population. The alternative hypothesis is that they are not the same. This test can also be one-tailed if the researcher has good reason to expect the difference goes in a particular direction.

It helps to think of the dependent-samples t test as a special case of the one-sample t test. However, the first step in the dependent-samples t test is to reduce the two scores for each participant to a single difference score by taking the difference between them. At this point, the dependent-samples t test becomes a one-sample t test on the difference scores. The hypothetical population mean (µ 0 ) of interest is 0 because this is what the mean difference score would be if there were no difference on average between the two times or two conditions. We can now think of the null hypothesis as being that the mean difference score in the population is 0 (µ 0 = 0) and the alternative hypothesis as being that the mean difference score in the population is not 0 (µ 0 ≠ 0).

Example Dependent-Samples t Test

Imagine that the health psychologist now knows that people tend to underestimate the number of calories in junk food and has developed a short training program to improve their estimates. To test the effectiveness of this program, he conducts a pretest-posttest study in which 10 participants estimate the number of calories in a chocolate chip cookie before the training program and then again afterward. Because he expects the program to increase the participants’ estimates, he decides to do a one-tailed test. Now imagine further that the pretest estimates are

230, 250, 280, 175, 150, 200, 180, 210, 220, 190

and that the posttest estimates (for the same participants in the same order) are

250, 260, 250, 200, 160, 200, 200, 180, 230, 240.

The difference scores, then, are as follows:

+20, +10, −30, +25, +10, 0, +20, −30, +10, +50.

Note that it does not matter whether the first set of scores is subtracted from the second or the second from the first as long as it is done the same way for all participants. In this example, it makes sense to subtract the pretest estimates from the posttest estimates so that positive difference scores mean that the estimates went up after the training and negative difference scores mean the estimates went down.

The mean of the difference scores is 8.50 with a standard deviation of 27.27. The health psychologist can now compute the t score for his sample as follows:

\[ t = \frac{8.5 – 0}{( \frac{27.27}{ \sqrt{10}})} = 1.11 \]

If he enters the data into one of the online analysis tools or uses Excel or SPSS, it would tell him that the one-tailed p value for this t score (again with 10 − 1 = 9 degrees of freedom) is .148. Because this is greater than .05, he would retain the null hypothesis and conclude that the training program does not increase people’s calorie estimates. If he were to compute the t score by hand, he could look at Table 13.2 “Table of Critical Values of “ and see that the critical value of t for a one-tailed test with 9 degrees of freedom is +1.833. (It is positive this time because he was expecting a positive mean difference score.) The fact that his t score was less extreme than this critical value would tell him that his p value is greater than .05 and that he should fail to reject the null hypothesis.

The Independent-Samples t Test

The independent-samples t test is used to compare the means of two separate samples ( M 1 and M 2 ). The two samples might have been tested under different conditions in a between-subjects experiment, or they could be preexisting groups in a correlational design (e.g., women and men, extroverts and introverts). The null hypothesis is that the means of the two populations are the same: µ 1 = µ 2 . The alternative hypothesis is that they are not the same: µ 1 ≠ µ 2 . Again, the test can be one-tailed if the researcher has good reason to expect the difference goes in a particular direction.

The t statistic here is a bit more complicated because it must take into account two sample means, two standard deviations, and two sample sizes. The formula is as follows:

\[ t = \frac{ M_{1} – M_{2} }{ \sqrt{ \frac{ SD_{1}^{2}}{n_{1}} + \frac{ SD_{2}^{2}}{n_{2}}}} \]

Notice that this formula includes squared standard deviations (the variances) that appear inside the square root symbol. Also, lowercase n 1 and n 2 refer to the sample sizes in the two groups or condition (as opposed to capital N , which generally refers to the total sample size). The only additional thing to know here is that there are N − 2 degrees of freedom for the independent-samples t test.

Example Independent-Samples t Test

Now the health psychologist wants to compare the calorie estimates of people who regularly eat junk food with the estimates of people who rarely eat junk food. He believes the difference could come out in either direction so he decides to conduct a two-tailed test. He collects data from a sample of eight participants who eat junk food regularly and seven participants who rarely eat junk food. The data are as follows:

Junk food eaters: 180, 220, 150, 85, 200, 170, 150, 190

Non–junk food eaters: 200, 240, 190, 175, 200, 300, 240

The mean for the junk food eaters is 220.71 with a standard deviation of 41.23. The mean for the non–junk food eaters is 168.12 with a standard deviation of 42.66. He can now compute his t score as follows:

\[ t = \frac{ 220.71 – 168.12}{ \sqrt{ \frac{41.23^{2}}{8} + \frac{42.66^{2}}{7}}} = 2.42 \]

If he enters the data into one of the online analysis tools or uses Excel or SPSS, it would tell him that the two-tailed p value for this t score (with 15 − 2 = 13 degrees of freedom) is .015. Because this is less than .05, the health psychologist would reject the null hypothesis and conclude that people who eat junk food regularly make lower calorie estimates than people who eat it rarely. If he were to compute the t score by hand, he could look at Table 13.2 “Table of Critical Values of “ and see that the critical value of t for a two-tailed test with 13 degrees of freedom is ±2.160. The fact that his t score was more extreme than this critical value would tell him that his p value is less than .05 and that he should fail to retain the null hypothesis.

The Analysis of Variance

When there are more than two groups or condition means to be compared, the most common null hypothesis test is the analysis of variance (ANOVA) . In this section, we look primarily at the one-way ANOVA , which is used for between-subjects designs with a single independent variable. We then briefly consider some other versions of the ANOVA that are used for within-subjects and factorial research designs.

One-Way ANOVA

The one-way ANOVA is used to compare the means of more than two samples ( M1 , M 2 … M G ) in a between-subjects design. The null hypothesis is that all the means are equal in the population: µ 1 = µ 2 =…= µ G . The alternative hypothesis is that not all the means in the population are equal.

The test statistic for the ANOVA is called F . It is a ratio of two estimates of the population variance based on the sample data. One estimate of the population variance is called the mean squares between groups ( MS B ) and is based on the differences among the sample means. The other is called the mean squares within groups ( MS W ) and is based on the differences among the scores within each group. The F statistic is the ratio of the MS B to the MS W and can therefore be expressed as follows:

Again, the reason that F is useful is that we know how it is distributed when the null hypothesis is true. As shown in Figure 13.2 “Distribution of the “ , this distribution is unimodal and positively skewed with values that cluster around 1. The precise shape of the distribution depends on both the number of groups and the sample size, and there is a degrees of freedom value associated with each of these. The between-groups degrees of freedom is the number of groups minus one: df B = ( G − 1). The within-groups degrees of freedom is the total sample size minus the number of groups: df W = N − G . Again, knowing the distribution of F when the null hypothesis is true allows us to find the p value.

Figure 13.2 Distribution of the F Ratio With 2 and 37 Degrees of Freedom When the Null Hypothesis Is True

The red vertical line represents the critical value when α is .05.

The online tools in Chapter 12 “Descriptive Statistics” and statistical software such as Excel and SPSS will compute F and find the p value. If p is less than .05, then we reject the null hypothesis and conclude that there are differences among the group means in the population. If p is greater than .05, then we retain the null hypothesis and conclude that there is not enough evidence to say that there are differences. In the unlikely event that we would compute F by hand, we can use a table of critical values like Table 13.3 “Table of Critical Values of “ to make the decision. The idea is that any F ratio greater than the critical value has a p value of less than .05. Thus if the F ratio we compute is beyond the critical value, then we reject the null hypothesis. If the F ratio we compute is less than the critical value, then we retain the null hypothesis.

Table 13.3 Table of Critical Values of F When α = .05

Example One-Way ANOVA

Imagine that the health psychologist wants to compare the calorie estimates of psychology majors, nutrition majors, and professional dieticians. He collects the following data:

Psych majors: 200, 180, 220, 160, 150, 200, 190, 200 Nutrition majors: 190, 220, 200, 230, 160, 150, 200, 210, 195 Dieticians: 220, 250, 240, 275, 250, 230, 200, 240

The means are 187.50 ( SD = 23.14), 195.00 ( SD = 27.77), and 238.13 ( SD = 22.35), respectively. So it appears that dieticians made substantially more accurate estimates on average. The researcher would almost certainly enter these data into a program such as Excel or SPSS, which would compute F for him and find the p value. Table 13.4 “Typical One-Way ANOVA Output From Excel” shows the output of the one-way ANOVA function in Excel for these data. This is referred to as an ANOVA table. It shows that MS B is 5,971.88, MS W is 602.23, and their ratio, F , is 9.92. The p value is .0009. Because this is below .05, the researcher would reject the null hypothesis and conclude that the mean calorie estimates for the three groups are not the same in the population. Notice that the ANOVA table also includes the “sum of squares” ( SS ) for between groups and for within groups. These values are computed on the way to finding MS B and MS W but are not typically reported by the researcher. Finally, if the researcher were to compute the F ratio by hand, he could look at Table 13.3 “Table of Critical Values of “ and see that the critical value of F with 2 and 21 degrees of freedom is 3.467 (the same value in Table 13.4 “Typical One-Way ANOVA Output From Excel” under F crit ). The fact that his t score was more extreme than this critical value would tell him that his p value is less than .05 and that he should reject the null hypothesis.

Table 13.4 Typical One-Way ANOVA Output From Excel

ANOVA Elaborations

Post hoc comparisons.

When we reject the null hypothesis in a one-way ANOVA, we conclude that the group means are not all the same in the population. But this can indicate different things. With three groups, it can indicate that all three means are significantly different from each other. Or it can indicate that one of the means is significantly different from the other two, but the other two are not significantly different from each other. It could be, for example, that the mean calorie estimates of psychology majors, nutrition majors, and dieticians are all significantly different from each other. Or it could be that the mean for dieticians is significantly different from the means for psychology and nutrition majors, but the means for psychology and nutrition majors are not significantly different from each other. For this reason, statistically significant one-way ANOVA results are typically followed up with a series of post hoc comparisons of selected pairs of group means to determine which are different from which others.

One approach to post hoc comparisons would be to conduct a series of independent-samples t tests comparing each group mean to each of the other group means. But there is a problem with this approach. In general, if we conduct a t test when the null hypothesis is true, we have a 5% chance of mistakenly rejecting the null hypothesis (see Section 13.3 “Additional Considerations” for more on such Type I errors). If we conduct several t tests when the null hypothesis is true, the chance of mistakenly rejecting at least one null hypothesis increases with each test we conduct. Thus researchers do not usually make post hoc comparisons using standard t tests because there is too great a chance that they will mistakenly reject at least one null hypothesis. Instead, they use one of several modified t test procedures—among them the Bonferonni procedure, Fisher’s least significant difference (LSD) test, and Tukey’s honestly significant difference (HSD) test. The details of these approaches are beyond the scope of this book, but it is important to understand their purpose. It is to keep the risk of mistakenly rejecting a true null hypothesis to an acceptable level (close to 5%).

Repeated-Measures ANOVA

Recall that the one-way ANOVA is appropriate for between-subjects designs in which the means being compared come from separate groups of participants. It is not appropriate for within-subjects designs in which the means being compared come from the same participants tested under different conditions or at different times. This requires a slightly different approach, called the repeated-measures ANOVA . The basics of the repeated-measures ANOVA are the same as for the one-way ANOVA. The main difference is that measuring the dependent variable multiple times for each participant allows for a more refined measure of MS W . Imagine, for example, that the dependent variable in a study is a measure of reaction time. Some participants will be faster or slower than others because of stable individual differences in their nervous systems, muscles, and other factors. In a between-subjects design, these stable individual differences would simply add to the variability within the groups and increase the value of MS W . In a within-subjects design, however, these stable individual differences can be measured and subtracted from the value of MS W . This lower value of MS W means a higher value of F and a more sensitive test.

Factorial ANOVA

When more than one independent variable is included in a factorial design, the appropriate approach is the factorial ANOVA . Again, the basics of the factorial ANOVA are the same as for the one-way and repeated-measures ANOVAs. The main difference is that it produces an F ratio and p value for each main effect and for each interaction. Returning to our calorie estimation example, imagine that the health psychologist tests the effect of participant major (psychology vs. nutrition) and food type (cookie vs. hamburger) in a factorial design. A factorial ANOVA would produce separate F ratios and p values for the main effect of major, the main effect of food type, and the interaction between major and food. Appropriate modifications must be made depending on whether the design is between subjects, within subjects, or mixed.

Testing Pearson’s r

For relationships between quantitative variables, where Pearson’s r is used to describe the strength of those relationships, the appropriate null hypothesis test is a test of Pearson’s r . The basic logic is exactly the same as for other null hypothesis tests. In this case, the null hypothesis is that there is no relationship in the population. We can use the Greek lowercase rho (ρ) to represent the relevant parameter: ρ = 0. The alternative hypothesis is that there is a relationship in the population: ρ ≠ 0. As with the t test, this test can be two-tailed if the researcher has no expectation about the direction of the relationship or one-tailed if the researcher expects the relationship to go in a particular direction.

It is possible to use Pearson’s r for the sample to compute a t score with N − 2 degrees of freedom and then to proceed as for a t test. However, because of the way it is computed, Pearson’s r can also be treated as its own test statistic. The online statistical tools and statistical software such as Excel and SPSS generally compute Pearson’s r and provide the p value associated with that value of Pearson’s r . As always, if the p value is less than .05, we reject the null hypothesis and conclude that there is a relationship between the variables in the population. If the p value is greater than .05, we retain the null hypothesis and conclude that there is not enough evidence to say there is a relationship in the population. If we compute Pearson’s r by hand, we can use a table like Table 13.5 “Table of Critical Values of Pearson’s “ , which shows the critical values of r for various samples sizes when α is .05. A sample value of Pearson’s r that is more extreme than the critical value is statistically significant.

Table 13.5 Table of Critical Values of Pearson’s r When α = .05

Example Test of Pearson’s r

Imagine that the health psychologist is interested in the correlation between people’s calorie estimates and their weight. He has no expectation about the direction of the relationship, so he decides to conduct a two-tailed test. He computes the correlation for a sample of 22 college students and finds that Pearson’s r is −.21. The statistical software he uses tells him that the p value is .348. It is greater than .05, so he retains the null hypothesis and concludes that there is no relationship between people’s calorie estimates and their weight. If he were to compute Pearson’s r by hand, he could look at Table 13.5 “Table of Critical Values of Pearson’s “ and see that the critical value for 22 − 2 = 20 degrees of freedom is .444. The fact that Pearson’s r for the sample is less extreme than this critical value tells him that the p value is greater than .05 and that he should retain the null hypothesis.

Key Takeaways

• To compare two means, the most common null hypothesis test is the t test. The one-sample t test is used for comparing one sample mean with a hypothetical population mean of interest, the dependent-samples t test is used to compare two means in a within-subjects design, and the independent-samples t test is used to compare two means in a between-subjects design.
• To compare more than two means, the most common null hypothesis test is the analysis of variance (ANOVA). The one-way ANOVA is used for between-subjects designs with one independent variable, the repeated-measures ANOVA is used for within-subjects designs, and the factorial ANOVA is used for factorial designs.
• A null hypothesis test of Pearson’s r is used to compare a sample value of Pearson’s r with a hypothetical population value of 0.
• Practice: Use one of the online tools, Excel, or SPSS to reproduce the one-sample t test, dependent-samples t test, independent-samples t test, and one-way ANOVA for the four sets of calorie estimation data presented in this section.
• Practice: A sample of 25 college students rated their friendliness on a scale of 1 ( Much Lower Than Average ) to 7 ( Much Higher Than Average ). Their mean rating was 5.30 with a standard deviation of 1.50. Conduct a one-sample t test comparing their mean rating with a hypothetical mean rating of 4 ( Average ). The question is whether college students have a tendency to rate themselves as friendlier than average.
• Practice: Decide whether each of the following Pearson’s r values is statistically significant for both a one-tailed and a two-tailed test. (a) The correlation between height and IQ is +.13 in a sample of 35. (b) For a sample of 88 college students, the correlation between how disgusted they felt and the harshness of their moral judgments was +.23. (c) The correlation between the number of daily hassles and positive mood is −.43 for a sample of 30 middle-aged adults.
• Research Methods in Psychology. Provided by : University of Minnesota Libraries Publishing. Located at : http://open.lib.umn.edu/psychologyresearchmethods . License : CC BY-NC-SA: Attribution-NonCommercial-ShareAlike

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