experiment number 276

When renegade scientist and evil genius Jumba Jookiba created the illegal genetic experiment designated 626, he planned to unleash this fearsome abomination on an unsuspecting galaxy. Little did he know that an Earth girl named Lilo would tame the creature she called Stitch. But no matter, for Jumba had created hundreds of other experiments which would soon wreak havok on an unsuspecting Earth. Fortunately, Lilo and Stitch are still on the trail of the wayward experiments, attempting to turn them from evil and give their lives purpose beyond mindless destruction.

Below is a list of Stitch's known "cousins," along with their powers and abilities. Some are deadly, some simply annoying, but every one needs a home.

NOTE: The information presented here is taken directly from the TV series. Additions, deletions, and amendments to this list can be found in the Disney Studios' Archives and related websites. Chalk up the differences, if you like, to Jumba's memory and record-keeping methods. After all, he's not the most stable being in the galaxy.

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Lilo & Stitch: The Series #222

Lilo & Stitch: The Series » Lilo & Stitch: The Series #222 - Remmy: Experiment 276 released by Disney on April 14, 2006.

Summary Short summary describing this episode.

Remmy: Experiment 276 last edited by cloudguy on 01/30/22 02:29AM View full history

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Experiment 276 - Remmy

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Great Update 👍

From what is actually my least favorite experiment

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11: Solubility and Borax (Experiment)

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• Santa Monica College

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• To learn about the solubility and solubility equilibria.
• To see how equilibrium constants change with temperature.
• To determine the values of \(\Delta H^o\), \(\Delta S^o\), and \(\Delta G^o\) for a reaction.

Have you ever wondered how certain mineral deposits form in the ground? Or why you can dissolve more sugar in hot water than in cold? It turns out these processes are related by a concept known as the chemical solubility-product equilibrium constant , or \(K_{sp}\).

Let’s consider a very familiar example of solubility. When table sugar, \(\ce{C12H22O11(s)}\), is added to water it dissolves until the solution becomes saturated. At this point the water contains as much sugar as it can hold and any additional sugar added falls to the bottom of the glass. If we write the chemical equation for the dissolution of sugar in water as,

\[\ce{C12H22O11(s) <=>[+ H_2O] C12H22O11(aq)} \label{1}\]

then the amount of sugar that can be dissolved at a particular temperature is given by the equilibrium constant expression,

\[ K_{sp} = [\ce{C12H22O11}] \label{2}\]

Here \([\ce{C12H22O11}]\) is the molar concentration of the dissolved sugar, and the subscript sp on the equilibrium constant stands for solubility product. The value of \(K_{sp}\) for a particular compound can be used to determine how much of that compound can be dissolved in solution. Notice that we do not include the concentration of the solid reactant in the \(K_{sp}\) expression because the concentration of a pure solid is essentially constant, and so we set it equal to unity.

What would happen to our saturated sugar solution if we were to evaporated off some of the water? In this case the concentration of the dissolved sugar, \(\ce{[C12H22O11]}\), would increase. However, because the solution is already saturated, this means that the concentration of dissolved sugar would now exceed the value of \(K_{sp}\) (or that \(Q_{sp} > K_{sp}\)). To return the system to equilibrium some of the dissolved sugar must precipitate out of the solution. Notice that this shift of the equilibrium position of Equation \ref{1} to the left in response to the increase in \(\ce{C12H22O11(aq)}\) concentration is also in accordance with Le Chatelier’s principle.

Mineral deposits such as sulfur, borax, and calcium carbonate (limestone) found around lakes beds form in a similar way. If due to drought, the diversion of water sources, or other reasons the influx of water to a lake diminishes, then the water level in the lake will begin to drop. This results in an increase in the concentration of dissolved solids in the lake water. As the concentration of dissolved minerals pass their solubility product equilibrium values, they begin to precipitate out. This typically results in the formation of bands of mineral deposits around the lake. If you travel through Death Valley and parts of the southwest United States you can see, "dry lakes," where colorful rings of mineral deposits such as sulfur and borax surround once-filled lake beds.

For ionic species the value of \(K_{sp}\) depends of the concentration of each of the ions present in solution. Consider the dissolution reaction of magnesium hydroxide, \(\ce{Mg(OH)2}\) ( s ), described by,

\[\ce{ Mg(OH)2 (s) <=> Mg^{2+} (aq) + 2 OH^{-} (aq)} \label{3}\]

where \(s\) is the solubility of \(\ce{ Mg(OH)2}\) ( s ) in moles per liter and \(2s\) is the solubility of \(\ce{OH^{-}}\) ( aq ).

The solubility product expression depends on the concentrations of both the \(\ce{Mg^{2+}}\) ( aq ) and the \(\ce{OH^{-}}\) ( aq ) ions formed and is given by:

\[K_{sp}= [\ce{Mg^{2+}}][\ce{OH^{-}}]^{2} = (s)(2s)^{2} = 4s^{3} \label{4}\]

To determine the value of \(K_{sp}\) for a saturated \(\ce{Mg(OH)2}\) solution, you can titrate an aliquot of the solution using a strong acid to measure the concentration of \(ce{OH^{-}}\) ( aq ). You can then determine the value of \(s\) from the relationship \(2s = [\ce{OH^{-}}]\) in Equation \ref{3}. Finally, you can substitute this value into Equation \ref{4} to obtain the value of \(K_{sp}\) for magnesium hydroxide at the observed temperature. Thus, one method of determining the value of \(K_{sp}\) is to titrate one of the species present in solution at equilibrium. In this experiment you will use a similar method to measure the value of \(K_{sp}\) at various temperatures for the dissolution of borax.

Borax is a boron-containing mineral that occurs in nature as a white powder composed of soft highly soluble crystals. Borax was first discovered by silk-road travelers in dry lake beds in Tibet and later found in the California and Nevada deserts of America. It is the active ingredient in some soaps and detergents, a flux used in soldering, an anti-fungal agent, and is even used as a texturizer in some prepared foods. It is also used in the manufacture of the thermoresistant borosilicate glass commonly marketed as, "Pyrex." You may be most familiar with borax because of the laundry additive brand, “20 Mule Team Borax.”

The empirical formula of the natural form of the mineral borax is \(\ce{Na2B4O7*10H2O}\) and its IUPAC name is sodium tetraborate decahydrate, but here we shall simply refer to it by its common name, “borax.” The structure of the tetraborate anion, \(\ce{B4O5(OH)4^{2-}}\), is shown in Figure 1. From this figure you can see that a more structurally accurate description of the borax molecule is given by the chemical formula, \(\ce{Na2B4O5(OH)4*8H2O}\), which is the one we shall use here.

Figure 1: The Tetraborate Anion, \(\ce{B4O5(OH)4^{2-}}\)

The chemical equation for the dissolution of borax may be written as:

\[\underbrace{\ce{Na2[B4O5(OH)4]*8H2O (s)}}_{\text{Borax}} \ce{ <=> 2 Na^{+} (aq) + } \underbrace{\ce{B4O5(OH)4^{2-} (aq)}}_{\text{Tetraborate Anion}} \ce{+ 8 H2O(l) } \label{5}\]

The tetraborate anion formed in solution is a weak base and reacts with water according to:

\[\ce{B4O5(OH)4^{2-} (aq) + 5 H2O(aq) <=> 4 H3BO3 (aq)+2 OH^{-}(aq)} \label{6}\]

The products of the reaction are boric acid, \(\ce{H3BO3}\) ( aq ), a weak acid, and the hydroxide ion, \(\ce{OH^{-}}\) ( aq ), a strong base.

We can determine the concentration of the tetraborate anion in solution (and thus the amount of dissolved borax) in the following way: First, we collect a sample of the saturated solution at equilibrium, being careful only to collect the liquid portion and not any of the solid borax in equilibrium with it. Next we titrate the hydroxide ions using a strong acid, say \(\ce{HCl}\) ( aq ). As the hydroxide ions are removed from solution by the acid, more tetraborate anions are converted into boric acid and hydroxide ions, in accordance with Le Chatelier’s principle. That is, as \(\ce{OH^{-}}\) ions are removed from solution by the strong acid, the reaction described by Equation \ref{6} will shift right. We can represent the overall reaction between the tetraborate anions and the hydronium ions formed from the strong acid, \(\ce{H3O^{+}}\) ( aq ), that occurs during the titration as:

\[\ce{B4O5(OH)4^{2-} (aq) + 2 H3O^{+}(aq)+H2O (l) <=> 4 H3BO3 (aq)} \label{7}\]

The reaction described by Equation \ref{7} will continue until we reach the equivalence point of the titration. At this point all of the tetraborate anions have been converted to boric acid and all of the \(\ce{OH^{-}}\) ions in the solution have been neutralized. Because all of the base has been neutralized the addition of more strong acid causes the pH of the solution to shifts sharply from basic to acidic. The presence of an acid-base indicator allows us to observe this shift and will serve to signal the endpoint of our titration. The indicator we will use for this titration is bromocresol green, which changes color from blue to yellow at a pH of 4.8 upon the addition of acid.

From the amount of acid required to reach the equivalence point, we can determine the concentration of tetraborate anion present in the sample of our saturated borax solution. From this concentration we can determine the value of \(K_{sp}\) for borax using a method analogous to that described previously for the dissolution of \(\ce{Mg(OH)2}\) ( s ). This is the method you will use in this experiment to determine the value of \(K_{sp}\) for borax.

In this experiment you will also observe how the solubility of borax varies with temperature. You know, for example, that sugar dissolves more readily and to a greater extent in warm water than in cold. You may also be aware that other substances dissolve less readily in hot water than in cold. For example, calcium carbonate, \(\ce{CaCO3}\) ( s ), a major component of boiler scale is less soluble in warm water than in cold, and so tends to precipitate out as a white crust on the walls of hot-water heaters, cookware, and teakettles. In this experiment you will study the dependence of \(K_{sp}\) for borax on temperature. You will then use your \(K_{sp}\) and temperature data to determine the values of \( \Delta H^{o}_{rxn}\), \( \Delta S^{o}_{rxn}\), and \( \Delta G^{o}_{rxn}\) for the dissolution reaction described by Equation \ref{5}.

We can equate the thermodynamic expressions:

\[\Delta G^{o}_{rxn}=\Delta H^{o}_{rxn}-T \Delta S^{o}_{rxn} \label{8}\]

\[\Delta G^{o}_{rxn}=-RT \ln K \label{9}\]

\[\ln K=-\frac{\Delta H^{o}_{rxn}}{R} \left(\dfrac{1}{T}\right) + \frac{\Delta S^{o}_{rxn}}{R} \label{10}\]

Equation \ref{10} is one form of the van 't Hoff equation that describes the temperature dependence of the thermodynamic equilibrium constant , \(K\), on temperature. The value of the thermodynamic equilibrium constant, \(K\), can be related to \(K_{sp}\) by,

\[K = \frac{K_{sp}}{Q^{o}_{sp}} \label{11}\]

where \(Q^{o}_{sp}\) is the standard reaction solubility product quotient, which has a numerical value of unity and units that are the same as those as \(K_{sp}\). Thus, \(K\) in this experiment is the numeric but unitless value of \(K_{sp}\).

Notice that Equation \ref{10} is linear and of the form \(y=mx+b\), where \(y= \ln K\), \(m=\Delta H^{o}_{rxn}/R\), \(x = 1/T\), and \(b = \Delta S^{o}_{rxn}/R\). Therefore, a plot of \( \ln K\) versus \(1/T\) should yield a straight from which the values of \( \Delta H^{o}_{rxn}\) and \( \Delta S^{o}_{rxn}\) for the reaction described by Equation \ref{5} can be obtained. We can then use Equation \ref{8} to determine the value of \( \Delta G^{o}_{rxn}\) for the reaction at 25°C.

These calculations assume that the dissolution reaction described by Equation \ref{5} is the only one occurring in solution. In fact, the actual dissolution process for most substances is almost always more involved than that idealized by their \(K_{sp}\) equilibrium expression. Nevertheless, from the data we obtain we will be able to make a wide variety of predictions about the solubility of borax and will gain a better understanding of the chemistry of dry lakes and the dissolution of minerals and other chemicals in solution.

Materials and Equipment

You will need the following additional items for this experiment: three 50-mL burets and buret stands 5.00-mL pipet, pipet bulb, bucket of ice, 0.5-M standardized \(\ce{HCl}\) solution (should be in the laboratory room), fine-tipped permanent black marker (one can be shared by the entire lab room)

General Safety

Students must wear safety goggles at all times. Use caution when working with the 0.5 M hydrochloric acid solution; be sure to rinse any area of skin that comes into contact with this solution well.

WASTE DISPOSAL: All solutions used in this lab must be disposed of in the proper chemical waste container.

CLEAN-UP: Borax crystals are difficult to remove from glassware and the bench tops once dry. Be certain to rinse all glassware that comes into contact with the borax thoroughly using warm water at the end of the experiment. Also be certain to wipe down any spills of the borax solution that occur using several rinses of warm water. Your lab instructor may chose to deduct points from any group leaving a “white crust” behind on their bench top!

Experimental Set-up and Procedure:

This experiment requires teams of at least 3 students

Note on Cleaning Glassware: Do NOT use soap to wash glassware for this experiment or you will be titrating the soap residue as well as the borax (soap is also a base). You should rinse all glassware several times with deionized water before beginning this experiment. You should clean and rinse three 250-mL Erlenmeyer flasks, an 800-mL beaker, two 400-mL beakers, a 150-mL beaker, a small test tube, two thermometers, a glass stirring-rod, the three 50-mL burets, and the 5.00-mL pipet before beginning.

A Note about the Order of Trials for this Experiment: It is critical that the trials in this experiment be performed in order from cold to hot , and not the reverse. This is because borax solutions tend to supersaturate on cooling, and so running the experiment from hot to cold may result in significant errors in your results.

Trial 1, 10°C:

• Rinse an 800-mL beaker with deionized water and then with about 5-mL of the 0.5 M standardized \(\ce{HCl}\) solution. Fill this beaker with about 500 mL of the standardized \(\ce{HCl}\) solution. Record the exact concentration of the standardized 0.5 M \(\ce{HCl}\) solution (as given on the reagent label) on your data sheet. Label this beaker, “standardized HCl.” You will use this as your standardized \(\ce{HCl}\) solution throughout the experiment. Should you require additional standardized \(\ce{HCl}\) solution, be sure to check that the concentration is the same as the one in your beaker. If it is different you will need to rinse and refill your buret with this new solution and repeat any titrations underway making note of the new concentration for your calculations.
• Rinse your three 50-mL burets using about 5-mL of the 0.5 M standardized \(\ce{HCl}\) solution from your beaker and then fill each buret with this solution. Be certain that there are no small air bubbles in the buret tips and that the tips are pressed in firmly and do not leak. Clamp each buret to a separate buret stand, making sure that they are vertical.
• Using the 5.00-mL pipet dispense exactly 5.00 mL of deionized water into a clean dry small test tube. The water should come to about halfway up the test tube. Using a permanent marker, mark this test tube exactly at the water line. Do this carefully as this tube will become your measuring standard for the entire lab! Now empty the calibrated small test tube and set it aside.
• Weigh approximately 10 grams of solid borax and place it into a 150-mL beaker. Fill this beaker to about the 75-mL mark with deionized water. The mass and volume used here need only be approximate.
• Using your glass stirring-rod, stir the borax solution you have made and place it into an ice- water bath. Insert your thermometer into the solution. Continue to stir the solution gently every minute or so as it cools. Allow the solution to cool to about 10°C.
• It is important that the borax solution be saturated in order to achieve equilibrium between the solid and dissolved phases. If you can see solid borax crystals at the bottom of the beaker you are at equilibrium; if not you should add some additional solid borax until you can see white crystals at the bottom of the solution. Later as you heat the solution you may need to add additional solid borax to achieve this condition.
• While your solution is cooling, set up a heating apparatus using a ring stand, a wire mesh, and a Bunsen burner. Light the burner and set a half-filled 400-mL beaker of deionized water on the apparatus. Label this beaker “hot water rinse.” Keep the temperature of this water between about 60°C to 80°C. You will need this hot deionized water throughout the experiment. Add more deionized water to this beaker as needed.
• When the saturated borax solution in the 150-mL beaker reaches about 10°C, stop stirring the solution and allow the borax crystals in the solution to settle. This takes about 2 minutes. After the solid has settled record the exact temperature of the solution to the nearest tenth of a degree on your data sheet.
• Grip the calibrated small test tube with the 5.0-mL mark that you prepared earlier with your wire test-tube holder. Carefully measure out exactly 5.0 mL of the 10°C saturated borax solution using the calibrated small test tube. Be certain to pour only the clear liquid from the top of the solution into your small test tube and not any of the solid crystals from the bottom. Quickly pour this solution from the calibrated small test tube into a clean rinsed 250-mL Erlenmeyer flask. Label this flask, “Sample 1.”
• Using your beaker tongs to grasp the hot beaker of deionized water, fill the calibrated small test tube to the brim with hot deionized water to dissolve any borax crystals that formed on the inside the tube and pour this into the 250-mL Erlenmeyer flask labeled “Sample 1.” Repeat this rinse once more to be certain that all the borax from the calibrated small test tube has been transferred to the 250-mL Erlenmeyer flask.
• Next measure out a second 5.0-mL sample of the 10°C saturated borax solution (checking that the temperature is still the same) using the calibrated small test tube and pour this into a second clean rinsed 250-mL Erlenmeyer flask. Label this flask, “Sample 2.” Again fill the small test tube to the brim with deionized water from the hot water bath twice to rinse the small test tube. Pour these two rinses into this 250-mL Erlenmeyer flask as well.
• Repeat this procedure with the third 250-mL Erlenmeyer flask, labeling it, “Sample 3,” so that you now have three 250-mL Erlenmeyer flasks, each containing a 5.0-mL sample of the 10°C saturated borax solution and some warm deionized water.
• Bring the volume of water in each of the three 250-mL Erlenmeyer flasks up to about the 50-mL mark by adding room temperature deionized water. These amounts do not need to be exact because adding more or less deionized water will not change the number of borax molecules in each flask.
• Add four drops of bromocresol green indicator to each of the three 250-mL Erlenmeyer flasks. The solutions in each should turn a light blue color. You are now ready to begin the first titration.
• Working as a team you should now titrate the contents of each of the three 250-mL Erlenmeyer flasks. Because of the number of titrations required for this experiment, you should perform this step in parallel with three team members simultaneously performing the three titrations. The titration endpoint occurs when one drop of the standardized acid changes the color of the solution from light blue to yellow. Be certain to record both the initial and final volumes of 0.5 M standardized \(\ce{HCl}\) solution required for each titration to the correct number of significant figures on your data sheet. It is suggested that you double- check all buret readings with other members of your team to be sure they are accurate.
• Rinse out the three 250-mL Erlenmeyer flasks with deionized water and refill the three burets with your 0.5 M standardized \(\ce{HCl}\) solution.

Trial 2, 30°C:

• Prepare a second hot-water bath using another 400-mL beaker and another burner. This will not be used for a rinse, so you may use tap water to fill this second bath. Place the 150- mL beaker containing the borax solution into this hot-water. You may need to clamp the 150-mL beaker in place to keep it from turning over in the bath. Allow the borax solution in the 150-mL beaker to warm to about 30°C. (Do not use the hot deionized water bath for this or you may contaminate the deionized rinse water with borax from the outside of the 150- mL beaker). Be certain there are still visible crystals at the bottom of the 150-mL beaker so that you know the borax solution is in equilibrium with the solid phase and thus still saturated. If necessary, add more solid borax to the solution so that white crystals are visible at the bottom of the beaker at all times.
• Be careful not to overheat the borax solution . If you allow the borax to heat past the desired temperature and then cool back down, you may get supersaturation of the borax. This could result in significant errors in your experimental results. Should you overheat the solution, either collect your data at higher temperatures, or discard the borax solution in the proper waste container and prepare a fresh one, being careful not to exceed the temperature desired the second time. (The later trials for this experiment do not need to be at exactly 30°C, 50°C, and 70°C, so long as they are somewhere around these values).
• When the saturated borax solution reaches about 30°C, stop stirring the solution and allow the borax crystals in the solution to settle as before. Then record the exact temperature of the solution to the nearest tenth of a degree on your data sheet.
• Using your calibrated small test tube, carefully measure out three 5.0-mL samples of the approximately 30°C borax solution into each of the three clean rinsed 250-mL Erlenmeyer flasks rinsing the calibrated small test tube twice with the hot deionized water following each measurement as before. Remember to collect these rinses in the corresponding Erlenmeyer flask as well. Once again be certain no solid borax from the bottom of the 150-mL beaker is transferred to the calibrated small test tube during this process.
• Again bring the volumes in each of the three 250-mL Erlenmeyer flasks to 50 mL using room temperature deionized water. Add four drops of bromocresol green indicator to each solution and titrate each as before. Record all your results on your data sheet.
• At the end of this step rinse out the three 250-mL Erlenmeyer flasks with deionized water and refill the three burets with your 0.5 M standardized \(\ce{HCl}\) solution.

Trials 3 and 4; 50°C and 70°C:

• Using the hot water bath, heat the borax solution to about 50°C for Trial 3 and then to 70°C for Trial 4, collecting three 5.0-mL samples and performing three indicator titrations at each temperature as before.
• You may need to add additional borax to the solution in the 150-mL beaker as you heat it to maintain equilibrium. Be certain that for each trial you can see crystals of solid borax at the bottom of the solution in the 150-mL beaker. Also be sure to allow the solution to settle for at least 2 minutes before collecting your 5.0-mL samples. Again, be careful not to overheat the borax solution between trials.

Dispose of all solutions in the proper chemical waste container. Wet a paper towel with some of the hot deionized water and use this to wipe down your bench top. You can also use this water to help rinse any borax out of your glassware. Be sure all these rinses end up in the proper waste container as well.

Return the equipment you borrowed to its proper location in the laboratory room or to the chemistry stockroom. Finally, be certain that there is no white film of borax remaining on your bench top before leaving the room.

Lab Report: Minerals, Dry Lakes, and Borax

Name: ____________________________ Lab Partner: ________________________

Date: ________________________ Lab Section: __________________

Experimental Data

Molarity of standardized 0.5 M \(\ce{HCl}\) solution (from label on bottle): __________________

10°C Titration Data: Measured Temperature (°C): _________________

• Average \([\ce{B4O5(OH)4^{2-}}]\): __________________
• Measured Temperature (K): __________________
• Show a sample calculation below for each of the calculated entries in your table for Sample 1: Do not forget to consider the stoichiometery of the acid-base titration reaction in your calculations; it is not 1:1.

Record your data and calculations for your other temperature trials in the three tables below. There is no need to show calculations for the entries in these three tables.

30°C Titration Data: Measured Temperature (°C): __________________

• Average \([\ce{B4O5(OH)4^{2-}}]\): _________________

50°C Titration Data: Measured Temperature (°C): __________________

• Average \([\ce{B4O5(OH)4^{2-}}]\):_________________

70°C Titration Data: Measured Temperature (°C): __________________

Data Analysis

Complete the data summary table below:

• Show a sample calculation below illustrating how you obtained the value of \(K_{sp}\) for the 10°C trail. Do not forget to consider the reaction stoichiometery given by Equation \ref{5}.

Use Excel to create a graph of \(\ln K\) versus \(1/T\) for your data. Your graph should have an appropriate title and labeled axes with an appropriate scale. Using the Excel trendline function, add a best-fit line to your plotted data and have Excel display the equation of this line and its R2 value on your graph. Submit this graph with your report.

• Use this graph to determine the values of \(\Delta H^{o}_{rxn}\) and \(\Delta S^{o}_{rxn}\) for the reaction described by Equation \ref{5}. Be certain to include the proper units for each! Show your calculations:
• \(\Delta H^{o}_{rxn}\) (and units):________________________
• \(\Delta S^{o}_{rxn}\) (and units):________________________
• Use the values of \(\Delta H^{o}_{rxn}\) and \(\Delta S^{o}_{rxn}\) you obtained to determine the value of \(\Delta G^{o}_{rxn}\) 25°C for the reaction described by Equation \ref{5}. Show your calculations below:
• \(\Delta G^{o}_{rxn}\) (and units):________________________
• Does the solubility of borax increase or decrease with increasing temperature? Use both your physical observations and your \(K_{sp}\) measurements to justify your response.
• Does your value of \(\Delta H^{o}_{rxn}\) suggest that the reaction for the dissolution of borax described by Equation \ref{5} is exothermic or endothermic? Explain why this result is consistent with your answer to Question 1 above.
• The value of \(\Delta G^{o}_{rxn}\) that you determined for the dissolution of borax at 25°C should be positive (if not you should check your work).Given that \(\Delta G^{o}_{rxn} > 0\),how do you explain that some of the borax actually dissolved at 25°C?
• A geologist removes a sample of water from a lake in Nevada where the water level has been slowly dropping over time. The sample is found to be 0.02 M in borax. The lake is circular in shape and is currently 500 meters in diameter and 50 meters deep. Use your experimental data to predict the level of the lake when borax will begin to precipitate on the shore if the mean temperature of the lake water is 15°C. Assume that the lake is equally deep at all points.

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1.6: Scientific Experiments

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• Page ID 16716

• Suzanne Wakim & Mandeep Grewal
• Butte College

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Seeing Spots

The spots on this child's tongue are an early sign of vitamin C deficiency , which is also called scurvy. This disorder, which may be fatal, is uncommon today because foods high in vitamin C are readily available. They include tomatoes, peppers, and citrus fruits such as oranges, lemons, and limes. However, scurvy was a well-known problem on navy ships in the 1700s. It was said that scurvy caused more deaths in the British fleet than French and Spanish arms. At that time, the cause of scurvy was unknown and vitamins had not yet been discovered. Anecdotal evidence suggested that eating citrus fruits might cure scurvy. However, no one knew for certain until 1747, when a Scottish naval physician named John Lind did an experiment to test the idea. Lind's experiment was one of the first clinical experiments in the history of medicine.

What Is an Experiment?

An experiment is a special type of scientific investigation that is performed under controlled conditions. Like all investigations, an experiment generates evidence to test a hypothesis. But unlike some other types of investigations, an experiment involves manipulating some factors in a system in order to see how it affects the outcome. Ideally, experiments also involve controlling as many other factors as possible in order to isolate the cause of the experimental results.

An experiment generally tests how one particular variable is affected by some other specific variable. The affected variable is called the dependent variable  or outcome variable. The variable that affects the dependent variable is called the independent variable. It is also called the manipulated variable because this is the variable that is manipulated by the researcher. Any other variables ( control variable ) that might also affect the dependent variable are held constant, so the effects of the independent variable alone are measured.

Lind's Scurvy Experiment

Lind began his scurvy experiment onboard a British ship after it had been at sea for two months and sailors had started showing signs of scurvy. He chose a group of 12 sailors with scurvy and divided the group into 6 pairs. All 12 sailors received the same diet, but each pair also received a different daily supplement to the diet (Table \(\PageIndex{1}\)).

Lind's experiment ended after just five days when the fresh citrus fruits ran out for pair 5. However, the two sailors in this pair had already fully recovered or greatly improved. The sailors in pair 1 (receiving the quart of cider) also showed some improvement, but sailors in the other pairs showed none.

Can you identify the independent and dependent variables in Lind's experiment? The independent variable is the daily supplement received by the pairs. The dependent variable is the improvement/no improvement in scurvy symptoms. Lind's results supported the citrus fruit cure for scurvy, and it was soon adopted by the British navy with good results. However, the fact that scurvy is caused by a vitamin C deficiency was not discovered until almost 200 years later.

Lind's scurvy experiment included just 12 subjects. This is a very small sample by modern scientific standards. The sample in an experiment or other investigation consists of the individuals or events that are actually studied. It rarely includes the entire population because doing so would likely be impractical or even impossible.

There are two types of errors that may occur by studying a sample instead of the entire population: chance error and bias.

• A chance error occurs if the sample is too small. The smaller the sample is, the greater the chance that it does not fairly represent the whole population. Chance error is mitigated by using a larger sample.
• Bias occurs if the sample is not selected randomly with respect to a variable in the study. This problem is mitigated by taking care to choose a randomized sample.

A reliable experiment must be designed to minimize both of these potential sources of error. You can see how the sources of error were addressed in another landmark experiment: Jonas Salk's famous 1953 trial of his newly developed polio vaccine. Salk's massive experiment has been called the "greatest public health experiment in history."

Salk's Polio Vaccine Experiment

Imagine a nationwide epidemic of a contagious flu-like illness that attacks mainly children and often causes paralysis. That's exactly what happened in the U.S. during the first half of the 20th century. Starting in the early 1900s, there were repeated cycles of polio epidemics, and each seemed to be stronger than the one before. Many children ended up on life support in so-called "iron lungs" (see photo below) because their breathing muscles were paralyzed by the disease.

Polio is caused by a virus, and there is still no cure for this potentially devastating illness. Fortunately, it can now be prevented with vaccines. The first polio vaccine was discovered by Jonas Salk in 1952. After testing the vaccine on himself and his family members to assess its safety, Salk undertook a nationwide experiment to test the effectiveness of the vaccine using more than a million schoolchildren as subjects. It's hard to imagine a nationwide trial of an experimental vaccine using children as "guinea pigs." It would never happen today. However, in 1953, polio struck such fear in the hearts of parents that they accepted Salk's word that the vaccine was safe and gladly permitted their children to participate in the study.

Salk's experiment was very well designed. First, it included two very large, random samples of children — 600,000 in the treatment group, called the experimental group , and 600,000 in the untreated group, called the control group . Using very large and randomized samples reduced the potential for chance error and bias in the experiment. Children in the experimental group were injected with the experimental polio vaccine. Children in the control group were injected with a harmless saline (saltwater) solution. The saline injection was a placebo. A placebo is a "fake" treatment that actually has no effect on health. It is included in trials of vaccines and other medical treatments, so subjects will not know in which group (control or experimental) they have been placed. The use of a placebo helps researchers control for the placebo effect . This is a psychologically-based reaction to a treatment that occurs just because the subject is treated, even if the treatment has no real effect.

Experiments in which a placebo is used are generally blind experiments because the subjects are "blind" to their experimental group. This helps prevent bias in the experiment. Often, even the researchers do not know which subjects are in each group. This type of experiment is called a double-blind experiment because both subjects and researchers are "blind" to which subjects are in each group. Salk's vaccine trial was a double-blind experiment, and double-blind experiments are now considered the gold standard of clinical trials of vaccines, therapeutic drugs, and other medical treatments.

Salk's polio vaccine proved to be highly successful. Analysis of data from his study revealed that the vaccine was 80 to 90 percent effective in preventing polio. Almost overnight, Salk was hailed as a national hero. He appeared on the cover of Time magazine and was invited to the White House. Within a few years, millions of children had received the polio vaccine. By 1961, the incidence of polio in the U.S. had been reduced by 96 percent.

Limits on Experimentation

Well-done experiments are generally the most rigorous and reliable scientific investigations. However, their hallmark feature of manipulating variables to test outcomes is not possible, practical, or ethical in all investigations. As a result, many ideas cannot be tested through experimentation. For example, experiments cannot be used to test ideas about what our ancestors ate millions of years ago or how long-term cigarette smoking contributes to lung cancer. In the case of our ancestors, it is impossible to study them directly. Researchers must rely instead on indirect evidence, such as detailed observations of their fossilized teeth. In the case of smoking, it is unethical to expose human subjects to harmful cigarette smoke. Instead, researchers may use large observational studies of people who are already smokers, with nonsmokers as controls, to look for correlations between smoking habits and lung cancer.

Feature: Human Biology in the News

Lind undertook his experiment to test the effects of citrus fruits on scurvy at a time when seamen were dying by the thousands from this nutritional disease as he explored the world. Today's explorers are astronauts in space, and their nutrition is also crucial to the success of their missions. However, maintaining good nutrition in astronauts in space can be challenging. One problem is that astronauts tend to eat less while in space. Not only are they very busy on their missions, but they may also get tired of the space food rations. The environment of space is another problem. Factors such as microgravity and higher radiation exposure can have major effects on human health and require nutritional adjustments to help counteract them. A novel way of studying astronaut nutrition and health is provided by identical twin astronauts Scott and Mark Kelly (Figure \(\PageIndex{3}\)).

The Kellys are the first identical twin astronauts, but twin studies are nothing new. Scientists have used identical (homozygotic) twins as research subjects for many decades. Identical twins have the same genes, so any differences between them generally can be attributed to environmental influences rather than genetic causes. Mark Kelly spent almost a full year on the International Space Station (ISS) between 2015 and 2016, while his twin, Scott Kelly, stayed on the ground, serving as a control in the experiment. You may have noticed a lot of media coverage of Mark Kelly's return to Earth in March 2016 because his continuous sojourn in space was the longest of any American astronaut at that time. NASA is learning a great deal about the effects of long-term space travel on the human body by measuring and comparing nutritional indicators and other health data in the twins.

• How do experiments differ from other types of scientific investigations?
• Identify the independent and dependent variables in Salk's nationwide polio vaccine trial.
• Compare and contrast chance error and bias in sampling. How can each type of error be minimized?
• What is the placebo effect? Explain how Salk's experimental design controlled for it.
• Fill in the blanks. The _____________ variable is manipulated to see the effects on the ___________ variable.
• True or False. In studies of identical twins, the independent variable is their genetics.
• True or False. Experiments cannot be done on humans.
• True or False. Larger sample sizes are generally better than smaller ones in scientific experiments.
• Why do you think it was important that the sailors’ diets were all kept the same, other than the daily supplement?
• Can you think of some factors other than diet that could have potentially been different between the sailors that might have affected the outcome of the experiment?
• Why do you think the sailors who drank cider had some improvement in their scurvy symptoms?
• Explain why double-blind experiments are considered to be more rigorous than regular blind experiments.
• Why are studies using identical twins so useful?
• Do you think it is necessary to include a placebo (such as an injection with saline in a drug testing experiment) in experiments that use animals? Why or why not?

Explore More

Watch this entertaining TED talk, in which biochemist, Kary Mullis, talks about the experiment as the basis of modern science.

Check out this video to learn more about conducting scientific experiments:

Attributions

• Scorbutic tongue by CDC, public domain via Wikimedia Commons
• Iron lung ward by Food and Drug Administration, public domain via Wikimedia Commons
• Mark and Scott Kelly by NASA/Robert Markowitz, public domain via Wikimedia Commons
• Text adapted from Human Biology by CK-12 licensed CC BY-NC 3.0

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Characters / Lilo & Stitch – Experiments

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Lilo & Stitch franchise character index Lilo Pelekai and Stitch (Experiment 626) | Major Characters | Minor Characters | Stitch! anime | Stitch & Ai Experiments : 0-Series | 1-Series | 2-Series | 3-Series | 4-Series | 5-Series | 6-Series

Jumba's experiments / Stitch's "cousins" in general

• The Ace : Ace (262); 627 is an evil version.
• Acrophobic Bird : Well, "Acrophobic Alien Pteranodon ". Slugger (608) is only ever seen flying once or twice in The Series , though the anime Stitch! shows him flying a little more often.
• A Head at Each End : Swapper (355).
• Aliens Speaking English : The experiments known to speak English fluently or proficiently (that have officially appeared in any Lilo & Stitch media) are Gigi (007), note  As she spoke in Leroy & Stitch Slick (020), Hertz Donut (022), note  Only appeared in the Stitch! anime Forehead (044), Squeak (110), Bragg/Flute (145), note  Only appeared in the Stitch! anime as well Bonnie (149), Clyde (150), Nosy (199), Ace (262), note  Only in the anime's English dub Wormhole (272), note  Also only appeared in the anime Remmy (276), note  Also only in the anime's English dub Spooky (300), note  Mainly provided he replicates someone else as part of a fear, such as appearing as Cobra Bubbles to Nani Heckler (322), Elastico (345), note  Implied in his debut in The Series as he responds to and says "cousin"; confirmed in the anime Manners (358), Phantasmo (375), note  Based on his possession of the Macki Macaw animatronic Chopsuey (621), note  Only appeared in the Stitch: Experiment 626 video game Angel (624), note  However, she does not speak English in The Series or Disney Magic Kingdoms Reuben (625), Stitch (626) , 627, note  Only in the anime, since in his original appearance, all he could say was "evil" and Leroy (629). In addition, Frenchfry (062) can speak fluent French while Carmen (123)—in the anime, at least—can speak fluent Spanish (even though her namesake spoke Portuguese).
• All There in the Manual : Averted; the credits for Leroy & Stitch lists the names of all of them , although they made some mistakes in that list . Granted, a good deal of them don’t appear and all we have to go by is their name.
• Ambiguous Robots : Tippy, an experiment from Comic Zone: Lilo & Stitch without any official number, seems to be this.
• Living Toys : At one point, he possesses Lilo's doll, Scrump.
• Haunted Technology : As a benevolent example of this trope, his episode ends with him possessing a restaurant's broken animatronic and finding his one true place entertaining customers.
• Attack Reflector : Slugger (608), who's designed to deflect projectiles with his tail.
• Artificial Family Member : OK, extended family members, but all of them count towards this. note  Although Stitch, being a title protagonist, gets this trope applied to him more than all the others. Jumba initially just created them to do various, usually "evil" tasks, but over the course of the franchise (mainly the films and first TV series), they all become "cousins" to one another as they join the Pelekai ʻohana .
• Badass Family : Considering that they are a family of "cousins" who all happen to be illegal genetic creations with unique powers. Leroy & Stitch , as well as a few episodes of The Series and the anime, show how badass they are when they work together towards a common goal.
• Batman Can Breathe in Space : The Series episode "The Asteroid" shows that the experiments can survive the vacuum of space.
• Barbershop Quartets Are Funny : Experiment 044 (Forehead) is designed to annoy people with his off-key barbershop quartet singing.
• Big Damn Heroes : Several of the experiments get these after they are turned good. Sparky gets several in Stitch! The Movie .
• Bioweapon Beast : Many experiments were bio-engineered for the purpose of combat, most notably the 6-Series.
• Canon Foreigner : The experiments from Comic Zone: Lilo & Stitch . The only officially canonized one is Coco (052), and the other ones with known names and/or numbers are Slobber (347), Hisee (531), and Tippy .
• Chained Heat : Link (251), who binds two or more incompatible people together with his goop, which dissolves in Earth mud.
• Counting Sheep : Drowsy (360) is a reference to this trope, being a sheep-like experiment who is designed to put people into a deep sleep by bleating.
• Clone Degeneration : Dupe (344), whose ability divides the target's power between them and their clones.
• Crippling Overspecialization : A common weakness for Stitch's cousins; they're usually made for one purpose and one purpose only, making it impossible for them to put their talents to use for anything else. For example, Slugger (608) can play baseball because of his power to deflect incoming projectiles. However, he cannot play basketball for the exact same reason.
• Cyborg : Cyber (000), who actually debuted in the Stitch! anime, thus leaving his status in the canon dubious. He didn't start out like this, but after Jumba ditched him due to his violent psychological instability, he somehow acquired a cybernetic eye with a drill for an ear, replaced his arms for cybernetic ones with different weapons for each, and replaced his two legs with three-bladed spider-like legs.
• Death Ray : Heat (609), who can shoot a heat ray from his forehead.
• Digitized Hacker : Glitch (223), who is designed to possess electrical equipment and turn them against their owners.
• Drill Mole : Digger (529), who looks more like a meerkat than a mole.
• Early-Installment Weirdness : In-Universe example: Shrink (001) was apparently named by either Jumba or Hämsterviel well before the events of the franchise, as shown by a newspaper with the headline, "Idiot Scientist Actually Creates Something!"
• An Ice Person : Slushy (523), who can cause snowstorms in places by blowing ice at the highest peak.
• Blow You Away : Blowhard (533) and Phoon (540). The former's designed to immobilize people by blowing them like kites while the latter's designed to cause windstorms.
• Dishing Out Dirt : Richter (513), who causes earthquakes by slapping his tail on the ground.
• Green Thumb : Sprout (509), which is a plantlike experiment.
• Magma Man : Yang (502), who can shoot lava from his humps.
• Making a Splash : Yin (501) and Cannonball (520). The former is designed to spray water, though she needs to get it from outside sources, while the latter causes tsunamis when he splashes into any body of water.
• Metal Muncher : Tank (586), who grows bigger with every piece of metal he eats.
• Playing with Fire : Splodyhead (619) and Melty (228). While both of them shoot/breathe fire, the latter is designed to melt non-organic material with it.
• Shock and Awe : Sparky (221), who can cause blackouts by overloading power sources with his electricity.
• Energy Weapon : Zap (603), who is a living laser beam .
• Epic Flail : Thresher (544), who can flail his tentacles around to destroy things.
• Babyfier is a fairy version of Mew with a baby theme and the cuteness turned up a notch, down to sharing the index number 151. Even its whole concept draws a parallel with Mew's representation of an animal fetus.
• Also Spike (319), who looks almost exactly like Togemogumon .
• Fictionary : The experiments' native language is called Tantalog, which is supposedly a cross between native Hawaiian, Chinese, and "Chezcreekian", the last one supposedly being an obscure language spoken by a minority of people in the rural parts of Chesterfield in North Derbyshire, England. That said, some of the phrases in Tantalog (at least based on fan-made dictionaries) seem to take inspiration from other languages including French, Arabic, and Hebrew, and are incongruent with some of the Tantalog spoken in the official media (which make liberal use of "ah" and "ee" sounds). (In actuality, Tantalog was The Series ' s creators' attempt to turn Stitch's gibberish into a somewhat consistent language.)
• Flat Characters : An overwhelming majority of the experiments, sadly. Some of them do get some semblances of a personality, but they're not fleshed out enough.
• Forced Sleep : Experiment 360 (Drowsy) is designed to make people fall into eternal sleep by just bleating at them.
• Foreshadowing : Their existence was first implied by Jumba lying to the United Galactic Federation all the way back in the prologue of the original film itself.
• "Freaky Friday" Flip : Swapper (355), who can do this to two or more people by staring at them, and only he can switch them back.
• The Ghost : Even though Jumba made over 600 experiments, we only see 125 of them note  with confirmed names, numbers, and functions; this number does not include the Chinese experiments in Stitch & Ai throughout all the films, the first two shows, and a few spin-off media; the rest of Stitch's "cousins" remain unseen to this day.
• Giant Enemy Crab : Shortstuff (297), although it's due to a growth ray.
• Gone Horribly Right : In the Stitch! anime, Jumba designed Experiment Zero, also known as Cyber, to be the ultimate killer monster, and he succeeded—a little too well, in fact, because the experiment proved impossible to control. Thus, Jumba was forced to imprison him on an icy planet far away.
• Gone Horribly Wrong : A few of the experiments' destructive qualities weren't intentional, but instead a result of something going wrong with their powers—Skip for example was meant to be a Mundane Utility to help Jumba reheat his leftovers by making time skip ahead ten minutes. Somehow, Skip ended up making ten years go by instead.
• Gotta Catch 'Em All : Their role in The Series is for Lilo and Stitch to find them, rehabilitate them, name them, and give them a purpose beyond evil or mischievous intents.
• Three of Forehead (044)'s heads can sing well. If his fourth head could also sing, then he would be a one-experiment barbershop quartet.
• Carmen (123) is a natural player of the maracas.
• PJ (133) can play the trumpet. The end of Stitch and the Planet of Sand shows that Squeak (110), Ace (262), and Nosy (199) can also play the trumpet.
• Bragg/Flute (145) is a very talented flutist and harmonica player.
• Sample (258) is excellent at maintaining rhythm by providing backbeats from the samples of sound he records, which makes him perfect for the band he's in.
• Elastico (345) can play the trombone.
• Swapper (355) can play the keyboard.
• Sprout (509) can play the drums.
• Richter (513), Kixx (601), and a green experiment believed to be Choppers (441) can play the bongos, with the first and third of them being able to play the instrument with their tails.
• Thresher (544) can play the marimba.
• Although Yaarp (613) does not play an instrument, he is able to perform with the horn on his head.
• Finally, all three of the main trio of experiments are highly capable musicians: Angel (624) has her natural singing ability, Reuben (625) can play the saxophone, and Stitch (626) is known for his guitar and ukulele talents.
• Honest John's Dealership : Slick (020), whose main function is to talk people into buying things.
• Hollywood Tone-Deaf : Forehead (044). Just read this quote below. Forehead's first three heads: We're evil 'cause we sing...! Forehead's fourth head: ANNOYINGLY OFF- KEEEY ! Stitch: (pulling down his ears) Sheesh!
• Hoist by His Own Petard : Snafu (120) whose main function is to sabotage enemy plans. This makes him frustratingly difficult to capture, with Jumba stating that the only way to do so is by accident .
• Finder has it nearly as bad; he was numbered 458 in his debut episode , which would have made him the only 4-Series experiment to be the experiment of focus for an episode. The Japanese encyclopedia also uses 458 for him. However, "Skip" and Leroy & Stitch both number him as 158, negating the 4-Series from having a major representative experiment.
• Instant Mass: Just Add Water! : Experiments deactivated into pod form are reactivated by coming in contact with water.
• Instant People: Just Add Water! : Likewise, especially since they are sapient beings.
• Involuntary Dance : Carmen (123) and the anime version of Sample (258).
• Lazy Bum : Lax (285)'s main function is to turn people (and machines) into this. His powers don't work on Gretchen , however, as she considers work to be relaxing.
• LEGO Genetics : All of the experiments were genetically engineered by Jumba, who took the DNA of several alien creatures and put them together to create super-powerful creatures of chaos, destruction, and mischief. Some of the creatures used to make Stitch in particular were shown in The Origin of Stitch short film.
• Literal Genie : Wishy-Washy (267) grants wishes literally, but only as long as he still has power left as indicated on his belly marking.
• Living MacGuffins : They are the primary focus of Lilo & Stitch: The Series , where the titular duo has to hunt them down and make them good.
• Living Props : Reformed experiments seen in The Series are usually just there to support the main ʻohana for various means. This goes double for some like Backhoe (040), whom we only ever see in this context, with no information on how they were previously caught and tamed.
• Love Potion : Hunkahunka (323), though his ability's more akin to Cupid's Arrow .
• MacGuffins : Especially as pods.
• Make a Wish : Wishy-Washy (267), although he grants wishes very literally.
• Meaningful Name : Every single experiment is named after their primary function.
• Me's a Crowd : Dupe (344), though the strength among the clones he makes is divided among them.
• Monster of the Week : In The Series , each episode focuses on tracking down and dealing with a new and unique member of this lot. The anime also has this trope but to a lesser degree and mainly during its second and third seasons.
• Monstrous Germs : Poxy (222) is an amoeba with limbs and a face that infects people’s bodies to give them humiliating illnesses, such as smelly feet, purple pimples, and uncontrollable burping.
• Muck Monster : Ploot (505) is a variation, he's not an actual blob of pollution, but he instead absorbs trash to grow larger and can flood cities by transforming his collected garbage into black sludge. There’s also Fudgy (054), who's made of chocolate.
• Mundane Utility : Lilo tries to find a place where an experiment can use its ability for good. In fact, the 0-Series was created for testing and/or household purposes, though some of them went horribly right ; Frenchfry (062) for example, was meant to be Jumba's personal chef. The thing ended up trying to eat him .
• Nightmare Weaver : Remmy (276) is designed to give people nightmares and trap them inside them if they wake up while he's still in their minds.
• 627 and 628; the former was because he could not be rehabilitated and the latter was because it was never activated, so Lilo and Stitch never dealt with it.
• Leroy was an inversion in his debut appearance by having a name but not a number. This ended in June 2020 when a Stitch & the Samurai side story established him as being Experiment 629.
• Non-Malicious Monster : Some experiments seem to be less "evil" and more acting on their primary functions. Indeed, some of them are friendly from the get-go, with the only obstacle to their reformation being that their functions are meant for evil purposes. Others are so non-malicious that they find their "one true place" all on their own (Elastico and Gigi being good examples).
• Olympus Mons : The Nigh-Invulnerable 627, the apocalypse-inducing 611, and Stitch himself. The 6-series is generally created with this trope in mind.
• Our Dragons Are Different : Melty (228).
• Person of Mass Destruction : El Fin (611). His primary function is to be the ultimate super weapon.
• Planimal : Stamen (103), whose ears are shaped like flowers that distract bees from pollinating real ones.
• Plant Aliens : Sprout (509).
• Pokémon Speak : Gotchu (031), Woops (600), and—in the anime—Checkers (029); Felix (010) and 627 are actually just One-Word Vocabulary , since the only words they could say ("Dirty!" and "Evil!", respectively) are not their names. note  However, Felix did say a couple other words in the anime episode "Stitch vs. Hämsterviel, Pt. I", and 627's vocabulary was expanded in his anime episode.
• Prehistoric Animal Analogue : Some of the experiments have traits of dinosaurs; Richter (513) resembles an Ankylosaurus and Slugger (608) resembles a Pteranodon , for example.
• Psycho Prototype : Cyber (000) from the Stitch! anime is this to, well, Stitch, as the first experiment Jumba created and designed to essentially be a living weapon, not dissimilar to Stitch's original purpose of destroying everything he touches. He even had all of Stitch's powers in his original form. However, Jumba was unable to control Cyber and had to imprison him.
• Reality Warper : Shoe (113) controls luck, and Launch (607) is said to be able to warp the fabric of time and space, eventually causing the entire universe to collapse upon itself.
• Ridiculously Cute Critter : Several experiments qualify as this; Mr. Stenchy (254), in particular, was specifically designed to be this so he can draw people in with his cuteness before releasing his stench.
• Rubber Man : Elastico (345) can stretch his limbs and contort himself as if he doesn't have bones.
• Because of the massive number of experiments that have not appeared, there are lots of fanfics about Lilo and Stitch capturing fan-made experiments. Even then, some fans like to make new experiments that are numbered after Stitch, 627, and Leroy.
• Interestingly, they were invoking this trope long before the series was even around. Even in the immediate aftermath of the first movie, the implication of 625 then-off-screen experiments meant 625 blank character slates and 625 stories to be told. Fanfic writers eagerly took up the challenge.
• Scary Scorpions : Plasmoid (617) resembles one, and he can shoot plasma balls from his tail.
• Secret Project Refugee Family : All of them were created in a lab by a mad scientist for destructive purposes, and are now (mostly) stranded on Earth. They don't all live under the same roof, but Stitch considers them all to be his ʻohana .
• Sequel Non-Entity : Not a single experiment from the first two shows' continuities appear in Stitch & Ai .
• Shrink Ray : Shrink (001) is designed to, well, shrink things, although he can also enlarge things.
• Topper's number (025) is a reference to the date of Christmas Day (December 25).
• Carmen (123) is based on and named after Carmen Miranda .
• Hunkahunka (323) is named after a line in "Burning Love" by Lilo's idol Elvis Presley .
• Experiment 452 (who never appeared in any work) is named Bob .
• Houdini (604) is obviously named after the famous escape artist .
• Stitch/626 (the original, ultimate, and successful culmination of Jumba's research and experimentation).
• The Flawed Prototype versions of Stitch: Cyber/000 (Jumba's original Bioweapon Beast Living Weapon that Went Horribly Right ), Woops/600 (all of Stitch's genetics and powers, but is afflicted with abhorrent klutziness), Chopsuey/621 (all of Stitch's genetics and powers, but not as skilled or competent), and Reuben/625 (all of Stitch's genetics and powers, plus better language capability, but also lazy, cowardly, and gluttonous).
• The Evil Counterpart versions of Stitch: 627 ( Superior Successor to Stitch with many more powers and completely evil) and Leroy/629 (a straight-up Evil Twin clone of Stitch)
• Others who are similar to Stitch physically, mentally, and powerwise, particularly among the 6-series: Sparky/221 (similar body type with an extra set of retractable arms), Kixx/601 (similar four-armed body), Yaarp/613 (similar four-armed body), Splodyhead/619 (similar body type, four legs instead of arms), and Angel/624 (a Distaff Counterpart of Stitch's line).
• Toilet Humor : The name of the unseen Experiment 444, Pooperson.
• The Transmogrifier : Morpholomew (316) can transform targets into other living organisms as long as he has photographic memory of the form he's transforming them into.
• Tuckerization : Jesstifer (354), named after The Series writer and executive producer Jess Winfield , who previously used the name as his online identity, particularly on TV Tome.
• Turned Against Their Masters : Implied with Felix and Frenchfry, who were originally supposed to be Jumba's personal cleaner and chef respectively, but due to errors in their programming, ended up trying to kill him.
• Voice Changeling : Spooky (300) changes his voice whenever he transforms.

• You Are Number 6 : Technically all of them to Jumba, which plays a role in why Lilo went on to name them all. 627 and 628 were never named, however; the former due to being made to be impossible to rehabilitate, and the latter due to neither Lilo nor Stitch having ever dealt with it.

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• Lilo & Stitch – Minor Characters
• Characters/Lilo & Stitch
• Lilo & Stitch – Experiments: 0-Series
• Characters/Disney Animated Canon

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276 Angel Number meaning

Numerology meaning of angel number 276, meaning of seeing angel number 276, is angel number 276 a manifestation number, spirituality meaning of angel number 276, love and relationships meaning of angel number 276, money and career meaning of angel number 276, twin flame meanings of angel number 276, biblical meaning of angel number 276, negative meaning of angel number 276, summary of angel number 276, search for an angel number, join our newsletter.

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Experiment 276

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Designation #: 276

Danger Level: Low

• 1 Appearance
• 2 Mechanics
• 5 Additional Notes

Appearance [ ]

Experiment 276 itself does not have an appearance, but the entities that inhabit Experiment 276 all have their own designs.

Experiment 276-A: Walter

• Experiment 276-A is very similar looking to Baldi, except it wears black pants, and has extremely wide empty eye sockets, with a single human eye inside the right socket.

Experiment 276-B: B is Real

• Experiment 276-B resembles It's a Bully, but with black hair, and a large hole in the area where its eyes should be. It has two small human-like eyes, and a black bar over its mouth that seems to resemble a wide open mouth. Experiment 276-B also has three arms, and lacks legs.

Experiment 276-C: Principal Apparition

• Experiment 276-C takes a resemblance to Principal of the Thing's upper half, and appears to be extremely large. Its features are slightly obscured by static, and its facial features can barely be made out due to high contrast.

Experiment 276-D: The Gramophone

• Experiment 267-D resembles an altered version of Playtime that wears black clothes. Instead of a head, a gramophone takes its place.

Mechanics [ ]

While in the Science Lab, the player can come across many copies of Experiment 276 in a cardboard box. The game can be picked up like a normal item, and used on any computer by right clicking. If the player uses Experiment 276, they will immediately be sent to Experiment 276's main menu. The game's title screen is a white screen with green text that is both bolded and italicized (the text reads "BALDI'S BASICS"). There are also two buttons on the bottom of the screen ("PLAY" and "EXIT" respectively). If the player clicks the play button, they will be sent to a schoolhouse-like building that resembles a much more pixelated version of the main schoolhouse. Experiment 276's map layout is extremely vast, with extremely long hallways and segmented rooms.

The gameplay of Experiment 276 is simple, collect 7 notebooks while answering questions from various subjects, and then leave the schoolhouse. However, doing so is very difficult, as there is a lot of danger that may stand in the player's way.

• Experiment 276-A will replace the in-game version of Baldi at some point during gameplay, and becomes much more aggressive the longer the player is playing the game. Experiment 276-A acts very similarly to Baldi, but has the ability to teleport to random rooms, and causes the player to experience hallucinations when he is near. Being caught by Experiment 276-A results in a game over.
• Experiment 276-B will replace the in-game version of It's a Bully at some point during gameplay. It is first encountered when the player enters what seems to be an "indoor forest". The walls are painted to resemble a forest, and green carpeting resembling grass will cover the floor. At the very end of the room is a well that is filled with water. If the player approaches the well, they will be able to see the reflection of Experiment 276-B behind them. Upon turning around, the player will be met with a dark void, with what appears to be Experiment 276-B standing far away from them. From here, the player can freely move around and approach Experiment 276-B. If they get relatively close to Experiment 276-B, the floor will disappear beneath the player, and they will be dropped into a vast ocean. Upon being dropped into the ocean, a message will appear on the player's screen that reads "LET'S SEE HOW YOU LIKE IT." After this message stays on screen for a few seconds, the player will be sent back to the game's title screen.
• Experiment 276-C will replace the in-game version of The Principal of the Thing during gameplay. It has a chance of appearing when the player enters any set of yellow double doors. When entering the room ,the player will see a long hallway that extends into the darkness. If the player attempts to exit the room, the wall attached to the door will extend as the player gets closer to it, making it impossible for the player to leave. Once this happens, Experiment 276-C will appear at the opposite end of the hallway, rapidly approaching the player as a droning sound will play. The sound will get louder as Experiment 276-C approaches, until it eventually gets unbearably loud. Once being touched by Experiment 276-C, the player will be sent back to the game's title screen.
• Unlike the previous anomalous characters present within Experiment 276, Experiment 276-D does not cause the player to die if they encounter it. Experiment 276-D takes the form of Playtime, and will act very similar to her. Unlike Playtime, the player cannot interact with it when touched, instead, Experiment 276-D acts as a noise distraction. Loud music interwoven with crying will emit from it at all times. Experiment 276-D will chase the player if it gets close to them. After a couple seconds of chasing the player, Experiment 276-D will get bored and leave.

If the player somehow manages to collect all 7 notebooks and escape the schoolhouse without getting caught, the player will receive 10,000 You Can Think Points.

Gallery [ ]

Additional Notes [ ]

• Experiment 276 and its characters are based on the " Every Copy of Super Mario 64 is Personalized " trend that circulated in 2020.
• 1 Experiment 341
• 2 Experiment 053
• 3 Hallucination 044

Angel number 276

The 276 angel number meaning - A complete guide to this powerful number. Discover what you can do to manifest its blessings in your life.

The angel number 276: What does it mean?

The angel number 276 represents our innate ability to tap into our higher selves for guidance.

If you see this sequence of numbers, it’s a message to follow your instincts as you embark on a new chapter of your life.

The vibration of the number 6 is one of caregiving and family unity. In this time of transition, this pattern of numbers represents a message of love and support from your angels.

The appearance of angel number 276 often coincides with the appearance of other angel numbers. The angel number 222 is an example of one of these.

The number 222 is a potent one that represents the potential for renewal and reinvention. Seeing this number is a message from the angels that you are making the right decisions and that good fortune is on the way.

I don’t get 777’s message. The 777 angel number is potent, ushering in fresh starts, divine direction, and personal fortitude.

How to use the stars to get what you deserve

93% of people don’t know this truth about their zodiac sign. You are not alone in feeling disconnected from your zodiac sign.

But don’t worry. Your life path can be unlocked by knowing this one single truth. I’ve got the perfect map. A secret I am willing to share with you. A proven way to unlock love and happiness for you.

A plan so enlightening. A personal blueprint towards success and happiness. Advanced yet simple. It starts with discovering the single truth about your zodiac sign. Find out more.

Angel number 276 and love: What does it mean?

Angel number 276 is a reminder to be yourself and let go of what no longer serves you.

Your guardian angels want you to know they may use those close to you to help you see how you limit yourself.

It’s possible to have this experience by “mirroring” or having other people’s actions trigger an inward epiphany.

Suggestions might be just as effective. Listen carefully to the advice of your friends and family.

Remember that angels would never intentionally harm you by saying something they knew would hurt you.

Angel number 276 conveys that although transitions might be unsettling, staying in the same place is much worse.

You have the ability to make the required adjustments in your life, and your angels want to remind you of that.

Potentially toxic friendships and romantic partnerships should be severed. Your angels will put you in touch with the correct people as soon as you take the necessary steps!

Angel number 276 is a message to be authentic to your principles in all of your relationships.

If your partner’s beliefs are at odds with your own, it may be time to end the relationship. Now is the moment to invest in the relationships that help you shine brightest and feel most confident.

It’s crucial to invest in the bonds of friendship and love that help you flourish. Cut ties with people who are poisonous and no longer helpful to you.

Relationships that help you flourish and feel good about yourself should be a priority right now.

Keep in mind that it’s fine to take your time in locating a connection that aligns with your ideals.

Angel number 276 and Twin Flame reunion

The appearance of angel number 276 in your life is a strong indicator that you are going to be reunited with your twin flame.

When you meet your twin flame, you may experience an instant connection since they are the other half of your soul.

Keep an open mind and an optimistic outlook when you meet your twin flame; otherwise, the relationship may be intense but short-lived.

Feel the love and adoration that is shared between you, and just go with the flow. If you allow it, this might become one of the most significant partnerships of your life.

Discover the secret method of how the ultra-wealthy find their soulmate

There is one man who China’s rich and famous trust when it comes to finding their soulmate.

His name is Mr. Wang. He grew up as a child prodigy, having psychic visions that caused him to black out. His mystical match-making abilities are sought out by China’s rich and famous! Thousands of people have found their true soulmate thanks to Master Wang’s gift.

After countless requests, Mr. Wang is finally offering his rare match-making gift to the world. Answer just a few simple questions, and Mr. Wang will draw you a picture of your soulmate. Find out what your soulmate looks like.

Angel number 276: Discover the meaning of Twin Flame separation

Angel number 276 may be a message from the universe that the time has not yet come for you to be separated from your twin flame.

This is a reminder that you are not alone on your spiritual path, even if your trip is challenging.

It’s a good indicator if you can have an open mind and pursue your goals. To prevent a twin flame breakup, one must be willing to explore the potential of romantic relationships with others.

Don’t let setbacks and anxiety keep you from reaching out to the person who could turn out to be your soulmate.

Always keep in mind that you have people who care about you and want the best for you. You can count on the assistance of countless angels and spiritual advisors.

With their support, you’ll have the confidence to pursue your goals and find lasting love. You can get the love you deserve if you just keep believing and adopt the appropriate mentality.

Angel number 276 - How to interpret its spiritual meaning

The throat chakra, also known as the fifth chakra, sits at the area of the throat and is the seat of one’s ability to communicate and express oneself.

This chakra facilitates spiritual communication by linking us to our ideal selves through our thoughts and words.

When this chakra is in harmony, we are not inhibited from being who we truly are and can listen to the guidance of our inner voice.

The world can change for the better because people like us aren’t afraid to speak up and be heard.

Located in the space between the eyes, the sixth chakra, sometimes known as the “third eye chakra,” controls our innate psychic talents, imagination, and intuition.

It’s the key that unlocks the door to knowledge and insight, allowing us to take in the big picture.

With a healthy third eye chakra, we may rely on our own wisdom and creativity to guide our choices. The universe’s innate intelligence can be tapped into for direction.

The crown chakra, also known as the seventh chakra, is the point of contact between human beings and the divine.

It aids us in connecting with our highest selves and discovering our life’s true calling. The ability to feel one with the cosmos and all that depends on the health of our head chakra.

Why you keep seeing angel number 276: The angels have a message for you

Angel number 276 appears in your life to reassure you that the angels are guiding you on your spiritual path.

This is evidence that your efforts are bearing fruit and that the angels are cheering you on. Don’t give up hope; this is a period of renewal and improvement.

The angels are with you, so keep doing what you’re doing. The angels are with you while you go through a period of profound spiritual development.

They want you to know that you’re on the correct track and that they’re here to back you up with this phone number.

Astonishing fact about the number 276

Highway 276 is a well-traveled road in the United States, running through parts of North Carolina, South Carolina, and Texas.

In the field of astronomy, 276 Adelheid is a large main belt asteroid. It was discovered by Johann Palisa in 1888 and is named after a female personal name from a calendar of names, tying the number 276 to the exploration of our solar system.

The number 276 in the Bible

According to 2 Corinthians 7:6, Paul expressed his extreme anguish while awaiting Titus’s return from his visit to the Corinthians. There were a number of things that caused Paul concern and anxiety.

One example is the harsh scolding letter he had Titus deliver to the Corinthians concerning his previous, unpleasant visit with them. He had commanded them to reprove a leader who was sinning.

Ashish Gupta

Ashish Gupta is an angel number expert who has been helping people to connect with their guardian angels for over 20 years. He is a firm believer in the power of angels and their …

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Angel Number 276: Meaning In Spiritual Growth, Numerology, And Changes Into Your Life

In the mystical universe of numerology, angel numbers act as messengers, whispering sacred secrets and guiding us on our life’s path.

Among these celestial symbols, guardian angel number 276 emerges as a beacon of spiritual growth, change, and enlightenment. It’s a number that holds deep spiritual significance, inviting us to unlock its mysteries and discover how it influences our lives.

Join me as we explore the magical essence of 276, unveiling its power to transform our spiritual journeys and worldly experiences.

Angel Number 276 Meaning And Symbolism

Angel number 276 is a blend of energies, each number bringing its unique vibe. The number 2 evokes balance, harmony, and fulfillment of your life mission. Number 7 resonates with spiritual awakening, wisdom, and psychic abilities, nudging you towards a path of enlightenment.

Lastly, number 6 represents love, family, and domestic bliss, grounding you in values and emotional depth. Together, 276 encourages you to blend these aspects, enriching your spiritual and earthly journeys. It asks you to trust the universe, maintain balance, and nurture both your soul and relationships with others.

The presence of angels indicates their desire to communicate with you and shed light on various aspects of your life. They want you to know that seeing the number 276 is not mere coincidence; it’s a message carrying significant power. 276 serves as a reminder, particularly regarding matters of the heart and the love life.

Delving into the meaning of angel number 276, it encourages balance and harmony across all areas of your life, symbolizing the potential for positive changes and opportunities that may come your way. Each number within the sequence contributes to a broader message from your angels, emphasizing the importance of maintaining equilibrium and remaining receptive to their guidance and support. 

Number 2 Meaning

Number 2 whispers of balance, partnership, and purpose. It’s like a dance of life, guiding you to harmonize your dreams with your duties.

Imagine two hands holding, symbolizing support and unity. This number encourages you to find your path, connecting deeply with others, and to feel the rhythm of your soul’s mission in the tapestry of the universe.

Number 7 Meaning

Number 7 sings with mystery and magic. It speaks of deep spiritual journeys, seeking truths hidden in shadows.

This number is a call to open your heart to learning, to understand the unseen forces shaping your destiny. It’s about growth in wisdom and connecting with a deeper, mystical reality.

Number 6 Meaning

Number 6 holds the warmth of home and love. It’s about caring for those close to us, creating a safe, loving environment.

This number teaches us the beauty of responsibility and the joy found in loving others. It ties our spiritual quest back to the earth, grounding us in family and love.

The 276 Angel Number Meaning Spiritually

The 276 angel number is a cosmic message, blending energies of growth, harmony, and love. It whispers to you to balance life’s spiritual and physical sides. Think of it as a guide on your life’s path, encouraging you to learn, to connect with others, and to nurture your soul and relationships.

This mysterious number invites you to trust in the universe, to find joy in love and learning, and to seek harmony. It’s a call to awaken to the magic of life, blending wisdom, support, and care into a powerful force for growth and happiness.

Seeing the number 276 is akin to receiving a message from the spiritual realm, indicating that changes in your life are on the horizon. Angel number 276 carries with it the wisdom to trust your intuition, encouraging you to recognize and embrace the love and support that surrounds you. The ubiquity of 276 everywhere serves as a powerful reminder to stay attuned to the guidance of your angels.

It is a call to have faith and trust in the divine plan, emphasizing the spiritual meaning inherent in each aspect of your journey. Additionally, 276 can be seen as a reminder to serve others and unlock your full potential, fostering positive changes in your life. This number serves as a message from the angels, urging you to remain steadfast and open to the transformative possibilities that lie ahead. 

Angel Number 276 Numerology Meaning

In numerology, the energy of each number in 276 adds uniquely to its message. The number 2 encourages partnership and harmony, 7 draws us to the mystical, urging us to seek deeper truths, and 6 brings in nurturing love, tying us to the earthly joys of home and family.

Together, in 276, the angels want to form a symphony of spiritual growth, fostering connections and nurturing our inner selves. It’s an angelic nudge to find balance, cherish deep bonds, and revel in both the seen and unseen wonders of our lives. This combination is a beacon for personal enlightenment and heartfelt relationships.

In every step of the way, the guidance of your angels is apparent, urging you to recognize the significance of acts of service in your life. Angel number 276 wants you to know that it is a powerful message, a combination of energies symbolized by the number 2, which signifies cooperation and balance. The repeated appearance of angel number 276 is not coincidental; it is a sign that angels are actively supporting you in your endeavors.

Use this number as a tool to manifest your desires, understanding that by aligning your actions with the guidance provided, your needs will be met, and you can achieve the goals you set for yourself. The angels encourage you to remain open to the transformative potential of this powerful number, embracing its influence as you navigate through life’s journey. 

Keep Seeing The 276 Angel Number In Twin Flame Relationship

When you keep seeing the angel number 276 in a twin flame relationship, it hints at a profound connection. This special pairing is about growing together and finding harmony.

The number 276 encourages you to learn from each other, deepen your bond, and help each other on your spiritual paths.

It’s like finding a mirror that reflects your soul, offering love and learning. This message says your relationship has a deeper purpose, to bring out the best in each other, reminding us of the joy and beauty in shared growth and support.

Biblical Meaning Of Seeing The Angel Number 276

In the Bible, numbers hold profound meanings, weaving into the fabric of divine messages. The angel number 276, though not explicitly outlined, reflects biblical teachings through its digits.

The number 2 symbolizes union and peace, often associated with the coming together of entities for a greater purpose. The number 7 is deemed holy, representing completion and spiritual awakening, as seen in the creation story.

Lastly, 6 speaks to human imperfection and the need for God’s care, akin to the sixth day when humans were created. Together, they suggest a path of spiritual fulfillment, unity, and divine love.

Meaning And Significance In Love And Relationship

In love and relationships, seeing angel number 276 is like receiving a secret message from the universe. It whispers to us about nurturing our connections, bringing us closer to our soulmates or reinforcing the bonds we share.

This enigmatic number guides us to cherish and deepen our relationships, encouraging us to express our true feelings and to understand each other on a spiritual level. It’s as if the stars themselves are aligning, urging us to foster love that’s not just deep, but also spiritual and healing, offering us a glimpse into the mystical side of love.

The number 276 is a message that holds significant power as a particular number that carries profound significance when it appears in your life. Your guardian angels express their love through the manifestation of this number, which is made up of the energies symbolized by angel number 276. The significance of angel number 276 is widespread, as it appears everywhere, serving as a sign from the spiritual realm.

This number acts as a powerful reminder of the energy of the number 6, which is associated with harmony and balance. Furthermore, number 276 is a powerful reminder that happiness and love are attainable, bringing positive changes into your life. Embrace the message of angel number 276 as it guides you towards a path of fulfillment and contentment.

Meaning Of 276 Angel Number In Money And Career

In the realm of money and career, the angel number 276 is a reminder that carries a mystical whisper of prosperity and growth. It suggests that your financial and professional paths are under the watchful eye of the universe, guiding you toward abundance.

By blending the spiritual insight of 7 with the harmonious energy of 2, and the earthliness of 6, it signals a period of balance. Opportunities for wealth are merging with personal values and hard work. This number encourages you to trust in your talents and the cosmic plan, ensuring that diligence and faith will lead to success and fulfillment.

Meaning Of The 276 Angel Number In Changes Into Your Life

The 276 angel number heralds significant changes, inviting you to trust the path unfolding before you.

It’s a cosmic signal that change, though often daunting, is a necessary step toward reaching your true potential and destiny. This number advises us to welcome these changes with an open heart, for they are aligned with our highest good.

By accepting and adapting, we allow ourselves to grow and evolve, guided by the stars and the unseen forces that wish us well. It’s a reminder that new beginnings are on the horizon, leading to fulfilling and enriched lives.

What does seeing angel number 276 repeatedly signify?

Seeing the 276 angel number frequently is a sign from the cosmos that you are on the right path. It symbolizes harmony, spiritual growth, and potential prosperity in your life. This frequent sighting urges you to maintain faith in the universe and in your abilities.

How does angel number 276 influence personal relationships?

In the realm of personal relationships, angel number 276 signifies a period of emotional healing and growth. It encourages building relationships based on spiritual understanding and mutual support, ensuring a deeper, more meaningful connection.

Can angel number 276 have a particular significance in dreams?

Yes, encountering angel number 276 in dreams can be a powerful message from the spiritual realm. It indicates that guidance and messages are being sent to you during your most receptive state of mind. This number in dreams asks you to heed the insights and intuitions that emerge in your waking life.

In the whispers of the universe, angel number 276 emerges as a beacon of hope and balance. It reminds us to cherish our spiritual path, fostering growth and deeper connections in our relationships.

This mystical number, appearing in dreams or daily encounters, guides us towards a harmonious blend of material and spiritual realms. By listening to its message, we align with the cosmic rhythm, inviting peace, gratitude, and prosperity into our lives.

Trust in the subtle energies of 276 to illuminate your path, guiding you towards a future brimming with spiritual fulfillment and universal love.

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